• Title/Summary/Keyword: Glucanex

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Effect of $Glucanex^{(R)}$ 200G on Yeast Cell Wall (효모의 세포벽에 대한 $Glucanex^{(R)}$ 200G의 영향)

  • 김광석;장정은;윤현식
    • KSBB Journal
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    • v.19 no.4
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    • pp.288-290
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    • 2004
  • The cell wall of fifteen yeast strains were treated with Glucanex$^{(R)}$ 200G that contained mainly ${\beta}$1,3-glucanase and some ${\beta}$1,6-glucanase. In our previous study it was found that the yeasts that are more resistant to Glucanex$^{(R)}$ 200G treatment contained more ${\beta}$-glucan than the yeasts that are less resistant to Glucanex$^{(R)}$200G treatment. By measuring the resistance of cell wall to Glucanex$^{(R)}$ 200G, the relative content of ${\beta}$-glucan in yeast cell wall could be estimated. The resistance of cell wall to Glucanex$^{(R)}$ 200G was measured by counting viable cell number after reaction with and without Glucanex$^{(R)}$200G. The resistance of fifteen yeast strains to Glucanex$^{(R)}$ 200G were presented.ere presented.

Protoplast Preparation and Regeneration from Young Hyphae of the Citrinin Producing Fungus Monascus ruber

  • Norlha, Tenzin;Lee, In-Hyung
    • Food Science and Biotechnology
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    • v.14 no.4
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    • pp.543-546
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    • 2005
  • Optimized conditions for protoplast preparation and regeneration from young hyphae of Monascus ruber were established. Heat shock treatment of spores gave rapid and synchronized germination. Spores collected from cultures grown for 7-8 days at $30^{\circ}C$ were germinated until over 70% germ tubes reached to 3-5 spore length. Enzymatic digestion of young hyphae was optimal with 50 mg/mL Glucanex in 0.1 M sodium citrate buffer containing 0.8 M mannitol as an osmotic stabilizer. Regeneration rate was around 10% when 0.8 M sorbitol was used as an osmotic stabilizer in regeneration medium. These conditions will be applied in genetic study of M. ruber that produces citrinin at high level and thus is good model strain for molecular genetic dissection of citrinin biosynthesis.

Selection of High Efficient Enzyme for Protoplasts Isolation from Mushrooms (버섯류의 원형질체 나출을 위한 고효율 효소 선발)

  • Kim, Jong-Kun;Kim, Jin-Hee;Kong, Won-Sik;Kang, Hee-Wan
    • The Korean Journal of Mycology
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    • v.38 no.1
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    • pp.21-24
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    • 2010
  • This study was carried out to select cell wall degrading enzymes for maximizing protoplast yield from Basidiomycetes. The protoplasts were released from spore suspension, mycelia cultured on cellophane membrane, and homogenized mycelia of Flammulina velutipes using commercial cell wall degrading enzymes. The highest yield of protoplasts was obtained from the homogenized mycelia treated with the enzyme combination of $Glucanex^R$ 200G and cellulase onozuka R-10. The protocol was also available for Pleurotus ostreatus, P. eryngii, and Hypsizygus marmoreus.