• Title/Summary/Keyword: Ginseng root-ginsenoside-preparative and semi-preparative liquid chromatography-fraction-ation-isolation-identification

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Isolation of ginsenosides Rb1, Rb2, Rc Rd, Re, Rf and Rg1 from cinseng root by high performance liquid chromatography

  • Paik, Nam-Ho;Park, Man-Ki;Choi, Kang-Ju;Cho, Yung-Hyun
    • Archives of Pharmacal Research
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    • v.5 no.1
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    • pp.7-12
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    • 1982
  • Ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf and Rg1 were effectively isolated from ginseng root by preparative liquid chromatography (LC) on two PrepPAK-500/c18 cartridges in series and semipreparative LC on a .mu. Bondapak cabohydrate analysis column, a .mu.Bondapak C18 column or a .mu. Porasil column. The identities of the isolated ginsenosides were confirmed by analytical high-performance liquid chromatography (HPLC) and infrared spectrophotometry. By this method large scale isolation of pure ginsenosides was efficiently accomplished.

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