• Korean Journal of Plant Tissue Culture
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• v.21 no.5
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• pp.303-308
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• 1994

Shoot apical meristem dome explants from cassava plants (Ghanaian local variety) produced somatic embryos at a frequency of 32% when cultured on MS medium supplemented with 2 mg/L 2,4-D. Somatic embryo segments formed secondary embryos at frequencies of up to 93% when cultured on medium containing 1 mg/L 2,4-D for 2 to 3 weeks. Since the somatic embryos were not capable of converting into plantlets, adventitious shoot were induced from the sliced embryo segments by culturing them on medium containing 0.1 to 5 mg/L BA. After 8 weeks of culture, numerous shoots formed on the segments at frequencies up to 100%. The shoots were rooted and successfully transplanted to potting soil.