• Title/Summary/Keyword: Geum-nam Kim

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Draft Genome Sequences of a Unique t324-ST541-V Methicillin-Resistant Staphylococcus aureus Strain from a Pig

  • Moon, Dong Chan;Kim, Byung-Yong;Nam, Hyang-Mi;Jang, Geum-Chan;Jung, Suk-Chan;Lee, Hee-Soo;Park, Yong-Ho;Lim, Suk-Kyung
    • Journal of Microbiology and Biotechnology
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    • v.26 no.4
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    • pp.799-805
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    • 2016
  • Methicillin-resistant Staphylococcus aureus (MRSA), the major causative agent of nosocomial infection, has also been reported from non-human sources. A sequence type (ST) 541 MRSA isolate designated K12PJN53 was isolated from a healthy pig in 2012. The genome of K12PJN53 consists of 44 contiguous sequences (contigs), totalling 2,880,108 bases with 32.88% GC content. Among the annotated contigs, 14, 17, and 18 contained genes related to antimicrobial resistance, adherence, and toxin genes, respectively. The genomic distance of strain K12PJN53 was close to the ST398 strains. This is the first report of the draft genome sequence of a novel livestock-associated MRSA ST541 strain.

${\ll}$영추(靈樞).근결(根結)${\gg}$ 에 대한 연구(硏究)

  • Geum Gyeong-Su;Jeong Heon-Yeong;Kim Nam-Su;Jang Jong-Yeol
    • Journal of Korean Medical classics
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    • v.13 no.1
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    • pp.74-101
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    • 2000
  • Geungyul(根結) means roots and nodes of meridians. If human body gets damaged by energy which each seasons has, the meridians get some troubles. Healing for symptom, meridians consist of root, stay, follow, inpouring and node of acupuncture points should be in harmony. 1. This chapter explain roots and node parts of each meridian, acupuncture points name and root, stay, follow, inpouring of acupuncture points which in arm and leg of three Yin and Yang. 2. The opening, closing, axis function, the symptom, and the needling of both meridians of Yin and Yang are explained. 3. The method that predicts the rise-and-fall of visceral energy and the time of death by using an order of pulse and intermittent pulse is explained. 4. It is explained that since the physical condition of the rich and the poor are different, the method using a needling for each person to be healed, such as the deep-and-shallow and the quick-and-slow, are different. 5. It is explained that the excess and the deficiency in the energy of shape should be considered when a acupuncture is given.

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The Characteristic of Formation CoSi2/Si Thin Film by the RF-Sputtering Method (RF-Sputtering법에 의한 CoSi2/Si 박막 형성에 관한 특성)

  • Cho, Geum-Bae;Lee, Kang-Yoen;Choi, Youn-Ok;Kim, Nam-Oh;Jeong, Byeong-Ho
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.59 no.7
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    • pp.1255-1258
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    • 2010
  • In this paper, the $CoSi_2$ thin films with thicknesses of about $5{\mu}m$ were deposited on n-type silicon (111) substrates by RF magnetron sputtering method using a $CoSi_2$ target (99.99%). The flow rate of argon of 50 sccm, substrate temperature of $100^{\circ}C$, RF power of 60 watts, deposition time of 30 minutes, and the vacuum of $1\times10^{-6}$ Torr. The annealing treatments of the $CoSi_2$ thin film were performed from 500, 700 and $900^{\circ}C$ for 1h in air ambient by an electric furnace. In order to investigate the $CoSi_2$ thin film X-ray diffraction patterns were measured using the X-ray diffractometer (XRD). The structure of the thin films were investigated by using scanning the electron microscope (SEM) were used for review. The surface morphology of the thin films was measured with a atomic force microscopy (AFM). Temperature dependence of sheet resistivity and property of Hall effect was measured in the $CoSi_2$ thin film.

Se-loss-induced CIS Thin Films in RTA Process after Co-sputtering Using CuSe2 and InSe2 Targets

  • Kim, Nam-Hoon;Jun, Young-Kil;Cho, Geum-Bae
    • Journal of Electrical Engineering and Technology
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    • v.9 no.3
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    • pp.1009-1015
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    • 2014
  • Chalcopyrite $CuInSe_2$ (CIS) thin films were prepared without Se- / S-containing gas by co-sputtering using $CuSe_2$ and $InSe_2$ selenide-targets and rapid thermal annealing. The grain size increased to a maximum of 54.68 nm with a predominant (112) plane. The tetragonal distortion parameter ${\eta}$ decreased and the inter-planar spacing $d_{(112)}$ increased in the RTA-treated CIS thin films annealed at a $400^{\circ}C$, which indicates better crystal quality. The increased carrier concentration of RTA-treated p-type CIS thin films led to a decrease in resistivity due to an increase in Cu composition at annealing temperatures ${\geq}350^{\circ}C$. The optical band gap energy ($E_g$) of CIS thin films decreased to 1.127 eV in RTA-treated CIS thin films annealed at $400^{\circ}C$ due to the improved crystallinity, elevated carrier concentration and decreased In composition.

Flavonoids from the Leaves of Ailanthus altissima Swingle and their Antioxidant Activity

  • Lee, Min-Kyung;Kim, Su-Yeon;Park, Ji-Hae;Lee, Do-Gyeong;Lee, Dae-Young;Kim, Geum-Soog;Kim, Yong-Bum;Han, Dae-Seok;Lee, Chang-Ho;Baek, Nam-In
    • Journal of Applied Biological Chemistry
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    • v.56 no.4
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    • pp.213-217
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    • 2013
  • Phytochemical studies on the leaves of Ailanthus altissima (Simaroubaceae) have not been reported previously. Thus, the authors isolated and identified secondary metabolites from A. altissima. Dried and powdered leaves were extracted with 80% aqueous methanol, and the concentrated extract was successively partitioned with ethyl acetate, n-butanol, and water. Four flavonoids were isolated from the ethyl acetate fraction through repeated silica gel and octadecyl silica gel column chromatography. Spectroscopic data including NMR, MS, and IR allowed for identification of the chemical structures as quercetin (1), afzelin (2), quercitrin (3), and isoquercitrin (4). This is the first report of the isolation of these compounds from A. altissima. The four isolated flavonoids 1-4 as well as solvent fractions (ethyl acetate, n-butanol, and water), were evaluated for DPPH radical scavenging activity.

Evaluation on Extraction Conditions and HPLC Analysis Method for Ginsenosides in Panax ginseng (인삼의 추출조건 및 진세노사이드의 HPLC 분석법 평가)

  • Lee, Kyeong Hee;Lee, Dae Young;Lee, Seung Eun;Nam, Ki Yeul;Hwang, Gwang Bo;Kim, Hyung Don;Lee, Jae Won;Choi, Je Hun;Ahn, Young Sup;Kim, Seung Yu;Kim, Geum Soog
    • Korean Journal of Medicinal Crop Science
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    • v.24 no.1
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    • pp.47-54
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    • 2016
  • Background : A new extraction method-heated ultrasonic extraction was qualitatively and quantitatively analyzed for the extraction of major ginsenosides from ginseng extract; this new high-performance liquid chromatography (HPLC) method was compared with the official extraction method of Korean industrial standards and standard for health functional food. Methods and Results : Ginsenoside compounds were analyzed for 35 minutes by the new HPLC analysis method using a Halo$^{(R)}$ RP-Amide column. The new HPLC analysis method was validated by the measurement of intra-day and inter-day precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ) of each ginsenoside. The correlation coefficients (r2) for the calibration curves of the ginsenoside compounds were over 0.9997 in terms of linearity. The heated ultrasonic extraction method using ultrasonication for 30 minutes at $50^{\circ}C$ yielded higher amount of ginsenosides than the extraction method of the Korean industrial standards owing to the enhancement of extraction efficiency. Conclusions : Compared to the other extraction methods, the heated ultrasonic extraction method yielded a higher amount of ginsenoside Rb1 than Rg1 index compounds for the quality evaluation of ginseng roots.

Analysis and Prediction for Spatial Distribution of Functional Feeding Groups of Aquatic Insects in the Geum River (금강 수계 수서곤충 섭식기능군의 공간분포 분석 및 예측)

  • Kim, Ki-Dong;Park, Young-Jun;Nam, Sang-Ho
    • Journal of the Korean Association of Geographic Information Studies
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    • v.15 no.1
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    • pp.99-118
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    • 2012
  • The aim of this study is to define a correlation between spatial distribution characteristics of FFG(Functional Feeding Groups) of aquatic insects and related environmental factors in the Geum River based on the theory of RCC(River Continuum Concept). For that objective we had used SMRA(Stepwise Multiple Regression Analysis) method to analyze close relationship between the distribution of aquatic insects and the physical and chemical factors that may affect their inhabiting environment in the study area. And then, a probabilistic method named Frequency Ratio Model(FRM) and spatial analysis function of GIS were applied to produce a predictive distribution map of biota community considering their distribution characteristics according to the environmental factors as related variables. As a result of SMRA, the values of decision coefficient for factors of elevation, stream width, flow velocity, conductivity, temperature and percentage of sand showed higher than 0.5. Therefore these 6 environmental factors were considered as major factors that might affect the distribution characteristics of aquatic insects. Finally, we had calculated RMSE(Root Mean Square Error) between the predicted distribution map and prior survey database from other researches to verify the result of this study. The values of RMSE were calculated from 0.1892 to 0.4242 according to each FFG so we could find out a high reliability of this study. The results of this study might be used to develop a new estimation method for aquatic ecosystem with macro invertebrate community and also be used as preliminary data for conservation and restoration of stream habitats.

Outpatient Antibiotic Prescription by Pediatric and ENT Physicians in Ulsan City (울산 지역 소아청소년과 및 이비인후과에서의 항생제 처방 형태)

  • Kim, Sung-Chull;Park, Yong-Chul;Kim, Bo-Geum;Nam, Doo-Hyun
    • Korean Journal of Clinical Pharmacy
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    • v.20 no.2
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    • pp.145-150
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    • 2010
  • In order to investigate the antibiotic prescription pattern for upper respiratory infections (URI), the prescription sheets for outpatients from July 2008 to June 2009 were collected from 7 community pharmacies in Ulsan City, and the prescription pattern of Pediatric and ENT physicians was analyzed. The antibiotic prescription rates of Pediatric and ENT physicians were 63.8% and 61.7%, respectively. It was also observed that the oral antibiotic prescription was 95.6% in Pediatrics and 97.6% in ENT. The most favorable antibiotics by Pediatric physicians were penicillins (21.5%) penicillin-clavulanate (36.4%) and cephalosporins (16.5%), macrolides (11.6%), quinolones (3.5%), and nifuroxazide (3.5%). In case of ENT, the commonly prescribed antibiotics were also penicillin-clavulanate (47.6%), cephalosporins (31.6%), macrolides (11.9%) and sulfonamide (1.3%). The antibiotic combination rate was 7.6% in Peditrics and 1.9% in ENT, among antibiotic prescriptions. The combination of more than two oral antibiotics was examined as 66.8% in Pediatrics and 44.2% in ENT. The common oral antibiotic combination in Pediatrics was prescriptions of two ${\beta}$-lactam antibiotics (54.3%). Among them 83% was the combination of amoxicillin-clavulanate (7:1) and amoxicillin, which could be judged as antibiotic overuse. The next highly prescribed oral antibiotic combination was ${\beta}$-lactam/macrolide antibiotic combination probably for URI (11.3%) and ${\beta}$-lactam/nifuroxazide combination (10.0%) presumably for acute diarrhea. Comparatively the oral antibiotic combination prescribed by ENT physicians was negligible except one physician. In conclusion, the antibiotic over-prescription rate by antibiotic combination was much higher in Pediatrics than ENT, even though both clinical departments showed nealy the similar antibiotic prescription rates.

Perception on Abortion among Korean Men in their 20s and 30s (2,30대 성인남성의 낙태에 대한 인식)

  • Kim, Geum-Nam;Kim, Kye-Ha
    • The Journal of the Korea Contents Association
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    • v.17 no.3
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    • pp.285-296
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    • 2017
  • The study was conducted to investigate how Korean men in their 20s and 30s perceive abortion. A cross-sectional survey was conducted. One hundred forty participants were selected from male adults in G city and J area. The inclusion criteria were as follows: (1) men were between 20 to 39 years old; (2) were able to read and understand Korean; (3) understood the purpose of the study; and (4) agreed voluntarily to participate in this study. A structured questionnaires were used as a study instrument, and included questions regarding subjects' general characteristics and their perception of abortion. Data were collected from March to June 2016. Descriptive statistics and Fisher exact test were used for analysis. 67.1% of participants responded that 'people can have abortion under some circumstances'. The results found that 'education about contraception (68.6%)' was widely regarding among the subjects as the best way to prevent abortion. There were also significant differences in their responses depending on a subject's some characteristics. Our results suggest that it is needed to offer education program about abortion for young men.