• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.172-172
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• 2017

Pollen germination and its' viability in bellflower hybrid system are of great importance. The present study was conducted to investigate the several factors underlying the pollen germination of Platycodon grandiflorum and obtain the basic data for effective artificial pollination for the production of sound specifies. The pollens of Platycodon grandiflorum started germination from one hour after planting, and the germination was actively progressed as time dependent manner. For lighting conditions, the germination of pollens under the light was faster by more than twice than that without the light. Furthermore, the germination was better in the high temperature rather than in the low temperature. The germination rate was higher in the $30^{\circ}C$. For the carbon source, the germination rate was better at the concentration of 15% regardless of the kinds. In particular, the highest value was observed with glucose. The germination rate was decreased substantially as the increasing with the higher pH. The dynamic germination of pollens was observed at the pH 5. With respect to the growth regulator, the higher concentration of NAA induced the higher the germination rate. $GA_3$ showed a good germination rate in $0.05mg{\cdot}L^{-1}$. Meanwhile, for kinetin, lower concentration increased the germination rate, unlike NAA. The higher concentrations of boric acid degraded the germination rate, and the addition of boric acid of $10mg{\cdot}L^{-1}$ demonstrated higher germination rate than the addition of other growth regulators. Notably, the addition of asparaginic acid exhibited the similar results in all test sectors regardless of concentration, whereas a little higher result was observed in the high concentration sector. Taken together, the results concluded that the boric acid was considered as one of the essential minerals that played an important role on the germination of pollens.

• Journal of Ecology and Environment
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• v.45 no.4
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• pp.174-181
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• 2021

Background: Euphorbia jolkinii Boiss. is a perennial species native to Jeju Island and the southern coastal area of Korea. Particularly on Jeju Island, the yellow flowers of E. jolkinii Boiss. have a high ornamental value because of their contrast with basalt. This study was conducted to investigate the effects of different temperatures (5, 15, 20, and 25 ℃) and gibberellic acid (GA₃) concentrations (0, 10, 100, or 1000 mg/L) on seed dormancy and germination of E. jolkinii. In addition, we classified the seed dormancy type and compared types with those of other species in the same genus. Results: The number of seeds with viable embryos and endosperms was approximately 66%. The final germination percentages at 5, 15, 20, and 25 ℃ were 51.7%, 83.5%, 2.6%, and 0.0%, respectively. In GA₃ concentration experiments, the final germination percentages of 0, 10, 100, and 1000 mg/L were 83.5%, 91.7%, 79.1%, and 83.4%, respectively, at 15 ℃ conditions, and 0.0%, 6.9%, 13.2%, and 27.3%, respectively, at 25 ℃. Conclusions: Germination improved at temperatures of 15 ℃ or lower. Furthermore, GA₃ treatment effectively reduced germination times. Thus, the seeds of E. jolkinni were classified as having non-deep physiological dormancy.

• Korean Journal of Medicinal Crop Science
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• v.1 no.1
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• pp.3-9
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• 1993

This experiment was conducted to study germination characteristics and the decrease cause of germination percent in Angelica gigas Nakai seed. The emergence percent of winter sowing was higher than that of spring sowing as 66.6% and 41.1%, respectively, and the first emergence date was also earlier in winter sowing. The seed germination speed, percent and coefficent showed the highest value at $20^{\circ}C$ of incubation temperature, but lower value at $10^{\circ}C\;and\;30^{\circ}C$. The water uptake speed was increased along with increasing water temperature. The weight of imbibed seed at germination was 3.4times higher based on the weight of intact dry seed and 2.3times on removal of seed coat. In terms of length of seed was large, the germination percent was higher. The germination percent of brown colored seeds showed higher value than that of green colored seeds. The prolonged storage period decreased germination percent. When A.gigas seeds stored at room temperatue for 2years, the seeds were lost their viability. The biological inhibition effect of methanol, water and ether extract on the germination and growth of A.gigas and lettuce seed showed the highest value in the methanol extract, followed by water extract and the least in ether extract.

• Journal of Forest and Environmental Science
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• v.29 no.4
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• pp.282-291
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• 2013

Rapid loss in viability of neem (Azadirachta indica A. Juss.) seed is a major problem. Present effort was undertaken for developing a set pattern for assessing of viability and vigour in seed of various mother tree age of neem (Age I-06 years, Age II-15 years, Age III-25 years and Age IV->30 years old). Various viability test viz. triphenyle tetrazolium chloride test, electrical conductivity, excised embryo test, and germination test have been performed on seeds obtained from mother tree age classes. Inconsistency was observed with the TTC and EC test in germination of seed in laboratory as well as nursery. While various vigour tests viz. cold test, chemical stress test (methanol stress test), and accelerated ageing test alongwith ageing index, germination test (G%, MGT and GV) and various seedling growth parameters like seedling length (cm), number of leaves, collar diameter (cm), total biomass (g) alongwith mathematical indices i.e. vigour index, sturdiness quotient, volume index, quality index, root shoot ratio in nursery as well have been taken for study and showed better consistency. On the basis present study results of various viability and vigour test indicated that mother tree age class II performed better in comparison to others and it can be recommended for seed collection. Further it is also recommended that viability of neem seed may be assessed using various laboratory tests like excise embryo test and germination test (G%, MGT and GV) and vigour test may be taken preferably by cold germination test, chemical (methanol) stress test, accelerated ageing test in laboratory and germination alongwith various seedling growth parameters seedling length (cm), number of leaves, collar diameter (cm), total biomass (g) alongwith mathematical indices like Vigour Index, Sturdiness quotient, Volume Index, Quality index, root shoot ratio in nursery as discussed in this study.

• Korean Journal of Plant Resources
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• v.22 no.6
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• pp.528-534
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• 2009

The study of germination and seedling growth characteristics of Neem under different soil and environment conditions was undertaken. The seed germination started 8 days after sowing in commercial bed soil, whereas, delayed germination was observed in sandy-loam (15 days) and sandy (19 days) soil. The highest germination (73.33%) was observed in commercial bed soil in green house, whereas, the lowest germination was observed in sandy soil (16.67%) and sandy-loam soil (8.33%). The seeds in the open field (sandy soil) also showed poor (10%) germination. The mean number of germination seed/day (GD) and seed germination vigor rate (GV) both were highest in the commercial bed soil with 0.733% and 16.67% respectively in the green house, whereas sandy and sandy-loam soil in green house and open field (sandy soil) all showed much lower GD and GV values. The seedling characteristics of nursery revealed that the seedling grown in the growth chamber in commercial bed soil was significantly higher in all the parameters comparing to others grown in green house and open field. The growth was nearly 7 fold in the chamber compared to that of the green house nursery observed in three months old seedlings. Likewise, HPLC analysis revealed that the green house grown seedling contain higher quantity of pigments compare to the chamber grown seedlings. Among the soils used the commercial soil alone or in combination with sandy and sandy-loam soil in the ratio of 2:1:1 respectively with the temperature of $27{\pm}2^{\circ}C$ showed better for Neem nursery preparation.

• Korean Journal of Plant Resources
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• v.29 no.2
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• pp.235-240
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• 2016

Factors affecting germination of seeds in the forms of various environment and chemical compounds. The present study was aim to produced effective seed propagation method of Astilbe koreana (Kom.) Nakai which had expected high value for the cut flower, ornamental and pharmaceutical material. Seed width and length ranged 0.62, 2.22 ㎜, respectively, and weight of thousand seeds was 40.5 ㎎. As result of imbibition test, showed moisture content of fresh seed (2.57%) increased rapidly by water-soaking treatment under 24 hours, recording to maximum value of 29.8%, and expansion of the seed coat was observed. Seed germination was the best at 15℃ and light conditions (40.8%) among temperature and light conditions treated. Percent germination of seeds was improved under the low (15, 20℃) than high temperature (25, 30℃). In addition, the seed was not germinated at dark condition regardless of temperature. Seeds of A. koreana thus seemed that it had low temperature germinability conditions. To improve germination rate, seeds were submerged in various concentrations of growth regulators such as GA₃ and kinetin, and minerals as KNO₃ and KCl. As a results, KNO₃ treatment, regardless of concentrations, promoted germination compared to control. Especially, percent of germination (77.8%), germination energy (96.1%), mean germination time (11.3 days) and T₅₀ (6.5 days) were effectively improved by treatment of KNO₃ 20 mM.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.54 no.3
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• pp.270-278
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• 2009

To understand the seed characteristics among cultivated types of Perilla crop and their weedy types in Korea and Japan, we studied the variation of 69 accessions by examining weight of 100 seeds, hardness of seed, germination percent and germination energy. The survey of the weight of 100 seeds and hardness of seed, clarified as follows; cultivated type of var. frutescens showed a range of 0.177 to 0.402 g with weight of 100 seeds, and most accessions of cultivated type of var. frutescens have soft seeds, except for several accessions, which have hard seeds, whereas weedy type of var. frutescens showed a range of 0.045 to 0.172 g with weight of 100 seeds, and has only hard seeds. While cultivated type of var. crispa showed a range of 0.054 to 0.101 g with weight of 100 seeds, and has only hard seeds. The weedy type of var. crispa showed a range of 0.059 to 0.135 g with weight of 100 seeds, and has only hard seeds. According to the result of germination tests, although the most accessions of cultivated and weedy types of var. frutescens and cultivated and weedy types of var. crispa showed below 50% germination rate at the first germination test, but some accessions of cultivated type of var. frutescens showed above 50% germination. While, in the second or third germination tests, most accessions of cultivated types of Perilla crop and their weedy types showed above 50% germination rates, except for several accessions. As a result, in this study, the average of germination percent and germination energy among accessions of cultivated types of Perilla crop and their weedy types showed respectively the highest value at the third germination test, but showed the lowest value at the first germination test. In addition, the germination percent and germination energy showed much higher in the room temperature condition than in $28^{\circ}C$ constant-temperature condition. Although our results may be required much clearly survey in the further study, this current results will be help for our understanding the variation of seed characteristics among cultivated types of Perilla crop and their weedy types in Korea and Japan.

• The Korean Journal of Ecology
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• v.13 no.2
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• pp.75-82
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• 1990

Toxic effert of water extract from leaves of Pinus rigida and some phenolic compounds on the seeds germination of Raphanus sativus var. hortensis for. acanthiformis Makino has been studied. There was little difference of germination percentage among the pH value of leaf extract (pH3~9). The germination percentage drastically with increased concentration level of leaf extract at about 60 percent. Seeds gemination of Raphanus sativus var. hortensis for. acanthiformis Makino inhibited severely by caffeic acid, ferulic acid and p-coumaric acid at M, but the germination percentage was higher than that of the control group in vanillic acid. In electrophoresis, there was no differences at earlier seedling stage of protein band between allelochemical treated and non-treated group, but in late stage, two protein band near 58kd and 27kd did not appeared in the toxic affected group. In case of caffeic acid treatment, two protein band near 58kd and 27kd did not found at late stage too.

• Korean Journal of Plant Resources
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• v.19 no.4
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• pp.480-487
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• 2006

This study was conducted to improve the germination percent and germination speed by seed priming using polyethylene glycol (PEG) 8,000 solution on Acer mono seed because it has low and irregular germination characteristics. Seed priming was carried out under 0, -0.25, -0.5, -1.0 and -2.0 MPa of water potential at $20^{\circ}C$ for 3 days. Primed seeds showed higher percent germination, germination speed, mean germination time (MGT) and germination performance index (GPI) than unprimed seeds. Especially, primed seed with -2.0 MPa of water potential had the highest germination properties. And primed seeds had a lower dormancy than the unprimed seeds. Relative growth rate and T/R ratio of seedling from primed seeds were measured to survey the effect of seed priming on the seedling vigor as well as seed germination. The seedlings from primed seeds at -2.0 MPa of water potential showed the highest relative growth rates of height and root collar diameter, however, the lowest value in T/R ratio. This study suggested that seeds priming at -2.0 MPa of water potential under $20^{\circ}C$ for 3 days enhanced not only the percent germination, germination speed, MGT and GPI but also the vigor and growth of seedlings in Acer mono.

• Journal of Practical Agriculture & Fisheries Research
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• v.23 no.2
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• pp.51-61
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• 2021

As a result of examining the germination rate between ginseng varieties, Jagyongjong varieties had the highest germination rate, and Yeonpung. had the lowest germination rate. In the ginseng seed germination rate experiment, the highest germination rate and growth condition were shown in artificial soil conditions of the ratio of Peatmoss 6.5: Pearlite 2: Masato 1.5. Good soil conditions require adequate soil moisture forces during the incubation period. The cultivation of ginseng medicinal crops requires optimal soil breathability, soil pH, and soil stabilization, which are important for root breathing. Microbial activity in the soil has a great influence on the growth of ginseng. The optimum pH of the soil for ginseng cultivation is 5.0-5.5 As a result of the experiment, the soil remained in an appropriate range after a month. In general, when the EC concentration value of the soil for ginseng cultivation is 0.2 mS/cm or more, growth deteriorates, and when the EC concentration value is 0.5 mS/cm or more, concentration obstacles such as root decay occur. As a result of the analysis, the higher the concentration value of EC, the more likely it is to interfere with ginseng growth.