• 한국자원식물학회지
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• 제17권2호
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• pp.75-81
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• 2004

본 연구는 산림 수목 종자를 대상으로 다양한 초저온 동결 조건을 적용하고, 초저온 저장 후에 나타나는 종자의 발아 특성 및 유묘의 생장 특성 조사하기 위해 수행되었다. 유리화 과정을 기초로 한 초저온 저장은 다릅나무 종자의 발아율을 크게 감소시켰으나, 초저온 저장 전 동결보호제 처리와 초저온 저장 후의 빠른 해빙은 종자의 발아율 감소를 완화시켰다. 초저온 저장 전 동결보호제 노출 시간은 종자의 발아율에 영향을 주었다. 즉 동결보호제 노출시간이 길어짐에 따라 종자의 발아율은 감소하였다. 그러나 초저온 저장 기간은 종자의 발아율에 영향을 주지 않았다. 또한 동결보호제의 노출 시간과 초저온 저장 기간은 유묘의 생장 특성에 영향을 주지 않았다. 따라서 수목 종자의 초저온 저장은 조직의 손상이나 변형이 없이 장기간 저장할 수 있는 현지 외 보존 기술로 적절하다고 판단된다. 그러나 산림 종자의 보다 효율적인 초저온 저장을 위해서는 각 수종의 종자 특성에 맞는 동결보호제 및 처리 기술이 개발되어야 할 것으로 판단된다.

• 원예과학기술지
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• 제34권3호
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• pp.363-371
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• 2016

Tabulis [Astilboides tabularis (Hemsl.) Engl.] has the potential for becoming a useful horticultural crop. This study was conducted to classify the seed dormancy types and to identify the germination conditions that improve the horticultural usefulness of this plant. We found that A. tabularis (Hemsl.) Engl. produced undifferentiated embryos and had a low germination rate of under 50%. In addition, water submersion led to moisture absorption. Therefore, A. tabularis seeds were designated as morphologically dormant. The germination rate was highest (86.3%) at $30^{\circ}C$ under light conditions. Higher temperatures generally led to an acceleration of the germination process, regardless of light condition. To improve the germination rate, seeds were submerged in various concentrations of growth regulators, such as $GA_3$ and kinetin, as well as minerals such as $KNO_3$ and KCl. An analysis of germination characteristics at $30^{\circ}C$ under light conditions revealed that treatment with 200 and $500mg{\cdot}L^{-1}GA_3$ led to an excellent germination rate of 97.0%. Treatment with $100mg{\cdot}L^{-1}GA_3$ led to a better germination rate than the control as well. However, treatment with kinetin, $KNO_3$, and KCl had no effect on germination, regardless of concentration.

• 한국환경농학회지
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• 제17권2호
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• pp.156-159
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• 1998

작물 초기생육에 대한 저선량 방사선의 효과를 보고자 벼, 콩, 들깨 종자에 저선량 $^{\gamma}$선을 조사하여 발아율과 초기 생육을 관찰하였다. 저선량 $^{\gamma}$선에 의한 작물의 생육초기 효과는 한정되었으나 작물과 생육특성에 따라 적정 선량은 다르게 나타났다. 벼종자의 발아율은 2Gy조사구에서, 유묘초장과 생체중은 0.5Gy에서 가장 높았으며,대두종자의 발아율과 유모생육은 4Gy에서 가장 양호하였다. 들깨종자의 저선량 $^{\gamma}$선 조사에 의해 생육이 다소 불량하였으나 발아율은 2Gy에서, 유묘초장과 생체중은 1Gy에서 증가효과를 나타내었다.

• 한국환경과학회지
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• 제30권1호
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• pp.77-85
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• 2021

This study was carried out to establish optimal conditions for breaking dormancy of Agastache rugosa O. Kuntze seeds. A series of experiments according to seed maturity and treatment with plant growth regulators were performed to improve germination percentage and synchronize germination of the seeds. In addition, it was conducted to test whether the useful effect of seed treatment before sowing leads to healthy seedling and early vigorous growth. The average seed size was 1.85 mm (length) × 0.82 mm(width). The seed size was much smaller than other vegetable seeds. Seeds colorappeared dark brown, the shape of the seeds was oval and the weight of 1,000seeds was 352.8 mg. The optimum germination temperature was 22℃. Light exposure during germination did not affect germination promotion, suggesting that A. rugosa seeds are a kind of dark germinating seeds. Seed dormancy lasted for 40 days after harvesting, and GA3 treatment of dormant seeds could break dormancy. There were significant differences in germination percentage and rate according to the maturity of seeds. The germination percentage of mature seeds was 10 - 18% higher than that of immature seeds, and germination rate was 2 days faster. GA3 treatment during growth regulator treatment improved germinability, but BAP or ethephone treatment did not. The optimal growth regulator concentration of for germination was the combination treatment of 100 mM GA3 ＋ 100 mM BAP.

• The Korean Journal of Ecology
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• 제12권4호
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• pp.219-236
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• 1989

The effect of salinity on the seed germination and seedling growth of Pinus densiflora for. erecta Uyeki were studied under the controlled conditions in the growth chamber. The seeds were sorted into three classes in weight such as large ($15.49\pm$1.63mg), medium($10.61\pm$1.38mg), and small ($6.57\pm$1.33mg) to determine the role of seed weight in germination and seedling growth of the pine. Polymorphic seeds of the pine were germinated an salinity range of 0 to 1.5% NaCl under various temperature ($10^{\circ}$ $-25^{\circ}$ with $5^{\circ}$C interval of constant temperature, and $10^{\circ}$ $-20^{\circ}$C, $15^{\circ}$ $-25^{\circ}$C of alternating temperature) in order to determine their germinability and seeding growth. In control plot, there was little difference of germination percentage among the seed weight classes, but in saline plot, the larger seeds generally had a higher percentage and rate of germination. There occurred synergistic interaction between salinity and temperature in the germination and the increase of temperature enhanced germination of seeds at the same salinity level. Alternating temperature regimes of $15^{\circ}$-$25^{\circ}$C yielded maximum germination and no germination was occurred at $10^{\circ}C$. The germination at alternating temperature showed higher germination percentage than at constant temperature. The percentage and of germination decreased drastically with increased salinity level to 1.00%; no germination was occurred at 1.50% salinity level. The growth fo sddelings from larger seeds was better than that of smaller seeds at the same salinity and temperature. The hypocotyl and radicle were more sensitive than cotyledon to the increased salinity stress.

• 한국작물학회지
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• 제66권3호
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• pp.183-189
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• 2021

볍씨 종자의 최아정도 및 최아 손상정도에 따른 출현율 및 초기생육변화를 분석한 결과는 다음과 같다. 1. 볍씨 최아 길이가 길수록 출현율은 다소 감소하였으며 생육온도가 18℃ 이상으로 높을수록 차이가 큰 것으로 나타났다. 2. 최아 길이가 길수록 초장신장 속도가 감소하였으며 생육온도가 21℃ 이상으로 높을 시 최아길이 3 mm에서 초장이 크게 감소하였다. 3. 최아길이에 따른 엽수변화는 없었으나 줄기 두께 및 줄기 건물중, 뿌리 건물중은 최아길이가 길수록 유의하게 감소하였다. 4. 최아 손상정도가 증가할수록 출현율은 유의하게 감소하였으며 생육온도가 높을수록 감소폭은 증가하으며 엽수변화는 없었으나 줄기 두께 및 줄기 건물중, 뿌리 건물중은 최아 손상도가 길수록 유의하게 감소하였다. 최아 길이 및 종자 손상정도별 뿌리 건물중과 줄기 건물 중 변화는 유의한 정의 상관관계를 보였다.

• 한국토양환경학회지
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• 제3권3호
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• pp.63-74
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• 1998

본 연구는 홍수퇴적토에 대한 토양 특성 분석과 75일 동안의 발아/생장 실험을 통하여 식재 토양으로서의 적용 가능성 여부를 관찰하고자 한다. 또한 각 시료의 입도 분포, 유기물 함량 등이 다르므로 원퇴적토와 탄천 모래의 적절한 혼합(2 : 1, 3 : 1)을 통하여 식물의 발아/생장 최적화를 비교하고자 한다. 결과를 살펴보면, 팬지의 경우 조성비(탄천모래 : 원퇴적토) 3: 1에서 발아/생장이 가장 잘 나타났고 금잔화의 경우 실트와 점토를 충분히 함유하는 원퇴적토에서 발아/생장이 가장 잘 나타남을 알 수 있다. 결론적으로 홍수퇴적토는 식물에 따라 적절한 배합이 이루어지면 식재 토양으로서의 가치가 높음을 알 수 있다.

• 한국약용작물학회지
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• 제22권6호
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• pp.435-441
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• 2014

This study was conducted to investigate the quality of seeds, the germination rates and the days required for germination, to examine the patterns of protein expressions during the germination and to improve the techniques of managing and storing seeds and viability of the seeds of Lithospermum erythrorhizon Sieb. et Zucc. After collecting and harvesting seeds, they were classified to white and brown colors of seed coat through testing their seed size, weight, and quality. The germination rates, the days required for germination, and the protein expressions were examined with different colors of seed coats, storing temperatures and durations by treating the different plant growth regulators and primings. One hundred seed weight of white color was heavier about 1.17 g than those of brown one about 0.81 g. The germination rates in white color of seed coat was higher, 3.05 ~ 5.75%, than those in brown one. Its rates were decreased with getting longer in storage durations. There was no big differences on germination rates between storage temperatures. The plant growth regulator of $GA_3$ and Kinetin was affected to improve the seed germination. $GA_3$ increased the seed germination clearly at 25 ppm level, while kinetin increased it gradually from 25 to 100 ppm levels. In germination by seed primings, PEG6000 made higher germination rate with increasing their levels, whereas $KNO_3$ increased the germination until 100 mM level and then decreased it with 200 mM unlike PEG6000. The protein expressed during the seed germination were appeared more and clearer bands in the seed after germination, especially 20 ~ 30 kDa, compared to those in the seed before germination. These results showing more and clearer bands were positively related to the germination rates which were different by seed colors, storage temperatures and durations, and plant growth regulators and primings.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2003년도 심포지엄
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• pp.38-42
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• 2003

This experiment was carried out the investigation of proper temperature of germination and growth responses of turf grass to different seedling ages. The results were as follows; 1. It was indicated that the proper temperature of germination was 30$^{\circ}C$ of day and 15$^{\circ}C$ of night, and day length was 14/10 hrs. 2. It was observed that 50 days of seedling was excellent with growth components such as number of tiller, length of tillering, number of node and internode length.

• 한국약용작물학회지
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• 제23권1호
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• pp.13-19
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• 2015

This study was conducted to improve the postharvest storage techniques of managing and storing seeds, to test qualities and viabilities of the seeds and to examine the germination rate and the protein expression of Achyranthes japonica Nakai. The seeds collected from different areas of Je-Cheon and Gwang-Ju were stored with different temperatures and durations. Two plant growth regulators and two seed priming were treated to investigate their effect on the germination rates and the days required for germination. The weight of one hundred seed collected in Gwang-Ju was heavier than those in Je-Cheon. Seed length collected in Gwang-Ju was also longer about 5.12 mm than those in Je-Cheon about 4.90 mm and seed width was longer in Gwang-Ju than those in Je-Cheon. The rates of seed germination in two different collection areas were higher about 2.9 to 13.0% in Gwang-Ju compared to those in Je-Cheon. Comparing its rates with the storing temperatures and durations, they were not clearly different in between $4^{\circ}C$ and $25^{\circ}C$ and they also were gradually decreased with getting longer storing durations. The germination rates treated by plant growth regulators were higher with $GA_3$ than those with Kinetin. The highest seed germination rate was appeared at 50 ppm of $GA_3$. Comparing its rates with different seed priming, they were relatively higher with $KNO_3$ than those with PEG6000. In protein expression patterns between before the germinating and after the germinating of seeds, more and clear bands were appeared in the seed after the germination compared to those before the germination of seeds, especially 10 ~ 20 kDa. These results showing more and clear bands were more clearly appeared in Gwang-Ju compared to Je-Cheon. Comparing the protein expression with plant growth regulators and seed primings, $GA_3$ was better expression than those with Kinetin and $KNO_3$ was better than those with PEG6000. More and clear bands were closely related to the germination rates of seeds and more detailed studies would be required.