• Journal of Korean Society of Forest Science
• /
• v.97 no.2
• /
• pp.135-139
• /
• 2008

Seed production and germination could be influenced by some factors. The picking time and climate factors are regarded as the elements to obtain sound seeds. We have observed the seed productivity and germination of seeds from trees of Chamaecyparis obtusa selected in a clonal seed orchard. Depending on picking time, various shapes from liquid material, something jellied to the fully matured one were observed. Germination aspects varied throughout the test days. After 20days of seeding in a glass petri-dish, germinal apparatuses appeared from the all seeds which had been picked from after at the end of August. The highest germination rate of about 30% was observed from the seeds picked from $20^{th}$ of September and $10^{th}$ of October. Seed production was about two times higher in 2005 than in 2006 and the average germination rate was also higher in 2005. We have also analyzed the effects of climatic factors about two consecutive years on seed productivity. Among the climatic factors, monthly sum of temperature and of precipitation were the main factors for maturation of Chamaecyparis obtusa seeds.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.44 no.4
• /
• pp.316-320
• /
• 1999

Nine accessions of red rices collected from different regions were tested for germination characteristics in relation to seed maturity and several storage conditions after harvest. No red rice seeds germinated at five days after anthesis (DAA). Wanjuaengmi (long-grain red rice) seeds at 10DAA germinated up to 30% of total samples, producing only abnormal seedlings, whereas 10D-AA-seeds of normal cultivar showed only 3.3% germinability. Some red rice seeds absorbed more water than cultivated varieties, and showed less decrease in germination rate than cultivated varieties when seeds were exposed at -1, -5 or -1$0^{\circ}C$ for up to 60 hours after soaking. Red rice accessions maintained more than 95% germination when stored. indoor for 120 days. Two short-grain red rices showed about 50% germination when overwintered in the field, while other long-grain red rices and cultivated rices germinated less than 10%. Germination of seeds overwintered in clay loam soil was lower than that in loam soil, and seeds on surface germinated less than those in 1~7cm depth.

• Korean Journal of Plant Tissue Culture
• /
• v.27 no.2
• /
• pp.137-142
• /
• 2000

Calli were induced from the explants of infloresence, petiole and leaf blade of Cnidium officinale on MS medium with 2.4-D, while embryogenic callus was induced only from inflorescence explants. Somatic embryos of 78 per explant were formed during subculture of the explants on medium without 2.4-D after culture on medium with 2 mg/L 2.4-D. Cotyledonary variation, cup-shaped cotyledon of 49% and other abnormal cotyledons of 13.5 % was observed on the somatic embryos. However this variation could be overcomed by the addition of activated charcoal onto culture medium. Somatic embryos at cotyledonary stage germinated on MS basal medium but the germination rate was very poor, blow 50 %. Somatic embryos on the medium with activated charcoal showed improved germinaton.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.44 no.1
• /
• pp.74-77
• /
• 1999

The object of this study is to determine the effect of priming on the germination ability and seedling emergence of aged soybean seeds in lab and field conditions. Artificial or natural procedure for seed aging was applied in this study. One seed lot was artificially aged for 3 to 5 days at 42$^{\circ}C$ with high relative humidity (nearly RH 100%), and the other one was stored at room temperature for 17 months. Aged seeds were osmoconditioned in -1.1 MPa polyethylene glycol 8000 (PEG) solution for 3 days at $25^{\circ}C$ and air-dried. When Danyeobkong was aged for 4 days average germination was 61.5%, however, this improved to 98.5% after the priming treatment. Improvement of seed germination by priming the aged seed was consistent with large seed sized Jangyeobkong cultivar, indicating that the priming was effective in enhancing seed germinability regardless of seed size. Priming aged seeds also resulted in good stand establishment in the field trials. Germination of aged seeds of Danyeobkong without priming was 17.0%, whereas that of primed ones was 66.4%.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.42 no.5
• /
• pp.571-578
• /
• 1997

The development of new approaches for wheat grain hardness assessment may impact the grain industry in marketing, milling and breeding. This experiment was to develop a new method for fast identification between softness and hardness, and for maintaining germinability of seed after measurement in wheat. Results from the comparisons of accuracy and significance between image analysis and conventional methods(NIRS and textrometer) were summarized. Data obatined from image analysis for grain hardness did not show any difference from those of the conventional methods. The protein content analyzed by micro-Kjeldahl method was significantly correlated with the grain hardness measured by image analysis, textrometer, and NIRS. The analysis for wheat grain hardness using image analysis may be used as an alternative method to the conventional methods. This method also takes the seeds after analysis can be utilized as breeding materials in early generations.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.17 no.4
• /
• pp.329-344
• /
• 1997

To improve germination and growth of forages of hill pasture seeds of several forages were osmotically primed with polyethylene glycol (PEG) under different PEG concentrations, treatment periods, and temperatures. Seeds were coated with several materials, and tested for germination. The best primed or coated seeds in germination tests were surface sow on hilly area. The results obtained are summarized as follows; 1. Osmotic priming with PEG accelerated germination of forages and reduced time taken for germination. 2. Germinability was best when the seeds of Tama ryegrass or orchardgrass were treated for 6-9 days at 30g PEG1100 ml water, red clover or white clover for 3, or 9 days in 20g PEG1100 ml water at $10^{\circ}C$. 3. Priming seeds of Tama ryegrass or orchardgrass in 30% PEG solution for 6 days, and seeds of red clover or white clover in 20% PEG solution for 6 days at $15^{\circ}C$ were most effective in germination, similar to priming at $10^{\circ}C$ . 4. Priming seeds of Tama ryegrass at 15C, and orchardgrass, red clover, or white clover at $10^{\circ}C$ were effective in germination than priming at other temperatures. 5. Osmotic priming with PEG accelerated germination of forages compared to coated seeds in Petri-dishes, while coated seeds germinated more slowly, but showed better emergence and superior growth to those of primed or intact seeds in the field. 6. Priming seeds increased yield slightly, and coating seeds significantly increased its yield on hilly area.

• Korean Journal of Plant Resources
• /
• v.21 no.3
• /
• pp.171-177
• /
• 2008

Variations in antioxidant capacity of leaves and characteristics of seed germination among five Hibiscus hamabo populations in Korea were evaluated. While the leaves of H. hamabo from Shincheon contained highest Na concentration, those from Hado and Ohzo does lowest. MDA content and SOD activity didn't show significant difference among populations, but total protein and anthocyanin content were significantly different among five populations. The filled seed rates of five populations were ranged from 92.0 to 98.7%, and the moisture contents of seeds from Shinyang and Soando were lower than that of R. syriacus (5.18%). The fresh weights of H. hamabo from Hado and Ohzo were the highest and those of seeds from Shinyang and Soando were the lowest. Significant variations among H. hamabo populations were detected for all seed germination characters: percentage of germination, average germination time and germination rate and uniformity. SOD activities of seeds from Onpyoung and Soando were the highest of five populations, and total protein concentrations of seeds in Ohzo and Shinyang populations were higher than those of other populations. H. hamabo is considered exposed to salt stress and all characteristics of seed germinations were inferior to other species.

• Journal of Forest and Environmental Science
• /
• v.36 no.4
• /
• pp.267-273
• /
• 2020

Diospyros mespiliformis is a highly valued and threatened tree species within the Sahelo-Sudanian zone of Africa, but its seed germination requirements under cultivation are not well researched. In a first experiment which aimed at determining germination response of seeds to dehydration, fresh seeds were dried at room temperature for 26 days during which their moisture content, their germinability, and their viability were monitored at two-day intervals. In the second experiment, 14 pre-germination treatments were tested for their effect on the germination of dried seeds. Results showed that fresh seeds had 52.7% moisture and achieved 97.7% germination. As seeds were dried, percentage germination gradually decreased with decreasing moisture content and reached 0% when moisture content had dropped to 18%. Meanwhile, seed viability remained at 100% over drying duration. Seeds that were not germinated after air dry also recorded 100% viability. The most effective treatment for inducing germination of dried seeds was scarification using 98% sulfuric acid for 30 min which resulted in 96.6% germination. This study reports for the first time in D. mespiliformis seeds a desiccation-induced dormancy which can be efficiently alleviated by acid scarification. This study provides useful information that will contribute to efficient management of D. mespiliformis seed resources for propagation.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.42 no.4
• /
• pp.435-445
• /
• 1997

The effects of temperature, prechilling, chemicals such as $GA_3$, IAA, kinetin and $KNO_3$ on the germinability of Foeniculum vulgare seed of medicinal plant were examined. In Foeniculum vulgare, the germination rate appeared to be around 50~60% or more in general, showing no differences in germination rate with different temperatures, promoting substances, physical or chemical treatments, and prechilling treatments. The observation of embryo under stereoscopic microscope for Foeniculum vulgare in Umbelliferae showed that seeds with or without embryo was almost the same in number. This result suggests that the lower rate of germination in this species is caused by embryolessness of seeds. The straight-shaped embryos as well as Y-shaped embryos were also observed. Foeniculum vulgare of medicinal plants in Umbelliferae were observed under scanning electron microscope, and did not show any opening problem near micropyle area. Final count should be made on 7th day of germination test.

• Journal of Life Science
• /
• v.12 no.6
• /
• pp.694-699
• /
• 2002

The present experiment was carried out to determine the effect of seed-coat softening by various chemicals, washing, chilling, and light treatment alone and in combination during desiccation of seeds on germination of gourd (Lagenaria siceraria Standl.) seeds. Potassium hydroxide, sodium hydroxide, acetone, acetonitrile, and acetamide were used as chemicals for the softening. Washing, prechilling, drying and light treatment of the softened seeds were done for a hour with tap water, for one or three weeks at 3$^{\circ}C$, 12 hr at 35$^{\circ}C$, and 14 hr a day at 3$0^{\circ}C$ with red light illumination or darkness, respectively. FR-yongjadaemok and FR-kunghap were used as test cultivars. Seed-coat softening with 10% acetone and acetonitrile for 1 hour enhanced and accelerated seed germination compared to the other chemicals and water imbibition. KOH treated for the softening was necessary to increase the germination rate of seeds for following chilling, but acetone did not influence their germinability regardless of chilling. Washing the softened seeds in tap water increased the germination rate in comparison with no-washing. During desiccation of the seeds sequentially treated with the softening, washing and chilling, red light treatment showed higher germination rate than dark one, suggesting that red light treatment are necessary during desiccation.