• 한국응용곤충학회지
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• 제36권3호
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• pp.270-276
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• 1997

5.0% micro-capsuled fenitrothion, 2.0% hydramethylnon, 또는 0.8% chlorpyrifos가 주요 활성 물질인 독먹이 제제의 방제효과를 알아보기 위하여 바퀴, Bfattella germanica (L)가 번식하고 있는 15개의 식당에서 8주동안 야외실험을 실시한 결과는 다음과 같다. 바퀴 성충에 대한 방제효과는 mIcro-capsuled fenitrothion 제제 (평균 82.8%)가 chlorpyrifos 제제 (평균 68.4%)보다 유의성 있게 높았으나 hydramethylnon 제제 (평균 86.1%)와는 거의 같은 효과를 보였다. 또한, 바퀴 자충에 대한 방제효과는 3가지 독먹이 제제가 거의 같은 유효성(평균 74.9- 82.8%)을 나타내였다. 독먹이 소모량을 조사한 결과, 8주 동안에 서식 바퀴에 의한 micro-capsuled fenitrothion 제제의 소모량은 0.11 g으로, 평균 90% 이상 남아 있었으나, hydramethylnon 제제는 바퀴에 의한 소비량(O.72g)이 현격하게 많아서 잔여량이 평균 57%로 나타났다. 이러한 결과는 hydramethylnon의 살충 기작의 특성, 유인먹이의 재료, 서식 바퀴의 밀도와 관계가 있는 것으로 추정되었다.

• Entomological Research
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• 제48권6호
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• pp.459-465
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• 2018

The present study evaluated the efficacy of Maxforce gel baits containing 0.01% fipronil (Maxforce), 0.05% fipronil (Maxforce Magnum), 1.0% clothianidin (Maxforce Impact), and 0.6% indoxacarb (Avion gel bait; all from Bayer Environmental Science, Clayton, CA, USA) against reference and wild strains (Mobasher, Andisheh, Tamin-e Ejtemaei, and Maskoni) of German cockroach collected in 2016. Test design and bait placement were according to World Health Organization (WHO) procedures for efficacy achieving a 95% mortality rate. Although cockroaches fed on all gel baits, gel baits containing clothianidin and indoxacarb were eaten at higher rates than gel baits containing 0.01% and 0.05% fipronil. The cumulative mortality rates reached 100% for all cockroach strains at 32, 28, 28 and 40 h after eating baits containing 0.01% fipronil, 0.05% fipronil, clothianidin, and indoxacarb, respectively. Efficacy (a 95% mortality rate) after exposure (consumption) of gel baits containing 0.05% fipronil and clothianidin was seen after 28 h, compared with 32 h for 0.01% fipronil and 40 h for indoxacarb. In conclusion, commercial gel baits have improved and may be more effective than spray formulations against German cockroaches.

• 생명과학회지
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• 제5권2호
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• pp.63-69
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• 1995

Cockroach antigen have been known as a cause of allergic disease. German ockroach(Blattella germanica L.) was chosen because it has the highest distribution range and poulation density. To identify the common and specific antigens of adult and larval stage of german cockroach, we made monoclonal antibodies which were confirmen by SDS-PAGE and EITB. Anti-B. germanica antibody producing hybridomas were 24 among the total 960wells. Only 4 hybridomas did not have cross reaction to other species of cockroach and hluse dust mites(Dermatophagodies farinae and D. pteronyssius). SDS-PAGE revealed about 20 bands from 90Kd to 15Kd to 15Kd. ETB showed specific antigens a6 60, 72 and 82Kd which were experimented by the culture supernatant of 4 selected hybridomas. Especially 60Kd coincided with a band of immunized mouse sera.

• 생명과학회지
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• 제5권2호
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• pp.7-7
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• 1995

Cockroach antigen have been known as a cause of allergic disease. German ockroach(Blattella germanica L.) was chosen because it has the highest distribution range and poulation density. To identify the common and specific antigens of adult and larval stage of german cockroach, we made monoclonal antibodies which were confirmen by SDS-PAGE and EITB. Anti-B. germanica antibody producing hybridomas were 24 among the total 960wells. Only 4 hybridomas did not have cross reaction to other species of cockroach and hluse dust mites(Dermatophagodies farinae and D. pteronyssius). SDS-PAGE revealed about 20 bands from 90Kd to 15Kd to 15Kd. ETB showed specific antigens a6 60, 72 and 82Kd which were experimented by the culture supernatant of 4 selected hybridomas. Especially 60Kd coincided with a band of immunized mouse sera.

• Parasites, Hosts and Diseases
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• 제47권1호
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• pp.31-36
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• 2009

Cockroaches have been recognized as a major cause of asthma. Bla g 4 is one of the most important German cockroach allergens. The aim of this study is to investigate IgE reactivity to the recombinant Bla g 4 (rBla g 4) in the sera of allergic patients and identify linear IgE binding epitope. For protein expression, full-length Bla g 4 (EF202172) was divided into 5 overlapping peptide fragments (E1: aa 1-100, E2: aa 34-77, E3: aa 74-117, E4: aa 114-156, and E5: aa 153-182). The full-length and 5 peptide fragments of Bla g 4 was generated by PCR and over-expressed in E. coli BL21 (DE3). The IgE binding reactivities of the full-length and peptide fragments were measured by ELISA using 32 serum samples of cockroach allergy. The sera of 8 patients (25%) reacted with rBla g 4. Four sera (100%) showed IgE-binding reactivity to full-length and peptide fragment 4, and 2 sera (50%) reacted with peptide fragment 2. One (20%) serum reacted with peptide fragment 3. The results of ELISA using overlapping recombinant fragments indicated that the epitope region was located at amino acid sequences 34-73 and 78-113, and major IgE epitope of Bla g 4 was located at amino acid sequences 118-152 of C-terminal. B-cell epitope analysis of German cockroach allergen Bla g 4 could contribute to the strategic development of more specific and potentially efficacious immunotherapy.

• 한국응용곤충학회:학술대회논문집
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• 한국응용곤충학회 2004년도 춘계 학술발표회
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• pp.181-181
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• 2004

• Animal cells and systems
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• 제8권Supplyment
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• pp.73-73
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• 2004

• 한국응용곤충학회지
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• 제33권2호
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• pp.74-80
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• 1994

바퀴에 대한 dichlorvos(DDVP) 약제로 11세대 누대 도태한 계통에 대한 저항성 유발정도, 교차저항성 및 효소활성을 조사한 결과는 반수치사농도 비교시 감수성에 비하여 8.58배 증가하였고 교차정항성은 chlorpyrifos, propowur, fenvalerate 와 permethrin 약제에서 각각 3.35, 4.09, 2.83 및 2.00배의 저항성을 보였으며 효소활성은 filter paper testdptjrka수성 대비 33%가 증가하였고 전기영동시험에서는 4개의 band인 esterase-1,2,4 및 7 band 가 확인되었다.

• The Korean Journal of Ecology
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• 제8권4호
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• pp.179-196
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• 1985

By using temperatures as a key variable, a simulation model was constructed to predict the size and developmental speed for the German cockroach population. The following three research steps were conducted to implement the individual simulation technique to represent the basic life system of the cockroach. First, informations on developmental periods and survival rates in each life stage were obtained through rearing experiments at five different temperatures. Secondly, biological parameters needed for modeling were obtained based on these rearing results. The logistic equation was applied to calculating the developmental speed, while the averages of survival rates were utilized as parameters determining population size. And thirdly, a basic life model was constratued in a stimulative framework in FORTRAN for predicting the populating development on the individual basis. For this purpose the biological characteristics, such as life stage, age in days, developmental speed, fecundity, etc., were assigned as an inherent attribute of the transactiion so that they could accompany each individual automatically all through the simulation. This gave the model flexibility and applicability in representing the isnect life system. The save memory space in computer programing, two files were utilized in translocating the individual informations each other as time proceeded. The developed model could be effectively used as a strategic tool in interpreting and managing the cockroach population. It was also suggested in this study that the individual simulation could efficiently serve as a basis to formulate a fundamental framework on which the advanced and complex life process could be built.

• Parasites, Hosts and Diseases
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• 제61권1호
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• pp.60-71
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• 2023

Cockroaches can cause allergic sensitization in humans via contact with their feces or frass. Antibiotics can affect concentration of major allergen and total bacteria production in German cockroaches (Blattella germanica). This study examined the ability of antibiotic-treated German cockroaches to induce allergic airway inflammation and the effect of antibiotics on their lipopolysaccharide and Bla g1, 2, and 5 expression levels. Specifically, we measured the ability of German cockroach extract (with or without prior antibiotic exposure) to induce allergic inflammation in human bronchial epithelial cells and a mouse model of asthma. Bacterial 16S rRNA and lipopolysaccharide levels were lower in ampicillin-treated cockroaches than in the control group. The Bla g1, Bla g2, and Bla g5 expression in ampicillin-treated cockroaches decreased at both the protein and RNA levels. In human bronchial epithelial cell lines BEAS-2B exposed to the ampicillin-treated extract, expression levels of interleukin-6 and interleukin-8 were lower than that in the control group. The total cell count and eosinophil count in bronchoalveolar lavage fluid was also lower in mice exposed to the ampicillin-treated extract than in those exposed to normal cockroach extract. Mouse lung histopathology showed reduced immune cell infiltration and mucus production in the ampicillin group. Our results showed that ampicillin treatment reduced the symbiont bacterial population and major allergen levels in German cockroaches, leading to reduced airway inflammation in mice. These results can facilitate the preparation of protein extracts for immunotherapy or diagnostics applications.