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Form and Embryonic Characteristics of Pedicularis hallaisanensis Seeds As Endangered Wild Species II-Class Using Host Plants (숙주식물을 활용한 멸종위기야생식물II급 한라송이풀 종자의 형태 및 발아특성)

  • Kim, Lim-Kyu;Park, Eun-Hee;Gang, GeunHye;Hwang, Boo-Yeong;Jung, Hyun-Jin;Kim, Min-Yong;Park, Jeong-geun;Park, Sam-Bong;Kim, Bong-Gyu;Choo, Gab-Chul
    • Journal of Korean Society of Forest Science
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    • v.108 no.3
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    • pp.290-295
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    • 2019
  • This study was carried out to investigate the possibility of establishing a reproductive system for the seed of Pedicularis hallaisanensis, which is in the endangered wild species II class in Korea. The seed of P. hallaisanensis is egg-shaped, and the seed coat is dark brown. The embryo was identified as a dwarf type by the seed section. The seed length was $0.47{\pm}0.07mm$, width $0.16{\pm}0.006mm$, and thickness $0.12{\pm}0.01mm$. The weight of one seed was $0.0003{\pm}0.0001mg$, and 1000 seeds weighed $4.59{\pm}0.02mg$. The degree of seed viability was 75.33% by the tetrazolium (TZ) assay. The highest germination rate of P. hallaisanensis seed was 71% after 4 weeks of storage at $4^{\circ}C$. However, the germination rate tended to decrease gradually over a longer storage period. The germination rates after 6 or 8 weeks of storage at $4^{\circ}C$ were 64% and 60%, respectively. We used two host plants, Artemisia princeps and Dendranthema zawadskii, to determine the effect of host plants on P. hallaisanensis seed germination. The germination of P. hallaisanensis mixed with A. princeps or D. zawadskii started at 53.5 and 62.5 days after sowing, respectively. We did not find any germination 164 days postsowing with both host plants. When A. princeps and D. zawadskii were used as host plants for P. hallaisanensis seed germination, P. hallaisanensis seed germination rates were 45.5% and 19.5%, respectively. The average time to germination was 70.2 days for A. princeps, and 46.8 days for D. zawadskii.