• Title/Summary/Keyword: Genetically modified Food

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Assessing weediness of herbicide tolerant genetically modified soybean

  • Ko, Eun Mi;Kim, Do Young;Kim, Hye Jin;Chung, Young Soo;Kim, Chang-Gi
    • Korean Journal of Agricultural Science
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    • v.43 no.4
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    • pp.560-566
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    • 2016
  • Imports of genetically modified (GM) soybeans (Glycine max) for food or feed consumption in Korea have been increasing. Although the cultivation of GM soybeans has not yet been allowed in Korea, the number of field tests for GM soybeans has also been rising. This study was conducted to investigate whether herbicide tolerant GM soybean can survive and persist in uncultivated environments when they escape from transportation routes or from isolated fields. Seeds of GM and non-GM soybeans and wild soybeans (Glycine soja) were buried in 2 and 15 cm soil depths and their viability was examined after 1, 2, 6, and 10 months. GM and non-GM soybean seeds completely lost their viability within six months of burial, whereas seeds of wild soybean maintained their viability during the study period. Seeds of soybean and wild soybeans that were sown on the soil surface germinated and grew to vegetative cotyledon stage. Seedlings of GM and non-GM soybean did not compete well with weeds, including Cerastium glomeratum, Alopecurus aequalis var. amurensis, Capsella bursa-pastoris, Conyza canadensis, Stellaria aquatica, and Erigeron annuus. Also, GM soybean did not survive through winter. However, wild soybeans competed well with the weeds and became dominant in August. Herbicide tolerant GM soybean is unlikely to persist under uncultivated environments and to become weeds.

Perceptions of Opinion Leaders on Environmental Health Hazards and their Management Policies in Korea -Focusing on the Genetically Modified Organisms and Endocrine Disruptors (여론 주도 집단의 환경보건 위해물질에 대한 인식도와 그 관리 정책에 관한 연구 -유전자재조합식품과 내분비계 장애물질을 중심으로)

  • Ahn, Jong-Ju;Paik, Nam-Won
    • Journal of Environmental Health Sciences
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    • v.31 no.5 s.86
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    • pp.431-443
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    • 2005
  • This study was performed to investigate the perceptions of the opinion leaders, such as government officials, researchers, NGO workers, and journalists on the risks of endocrine disrupters (EDs) and genetically modified organisms (GMOs) as well as the related policies on these two hazards or potential hazards. The opinion leaders generally considered the EDs as the most serious hazard among twenty-one environmental health hazards in Korea, and agreed that the EDs would continuously be the most serious hazard. On overall average, the GMOs were ranked the 11th among the twenty-one health hazards. Further investigation indicated that the GMOs were variously ranked by the group of respondents: they were ranked the 2nd by the NGO workers, the 7th by the journalists, the 9th by the researchers and the 11th by the government officials. In general, the respondents considered the dioxin as a hazard with the highest risk while the GMOs were considered less hazardous. The opinion leaders considered that although the risks of the GMOs and EDs were not fully verified, the risks should be controlled through the legislation. The EDs and GMOs should be separately regulated for the time being, while the EDs should put under more strengthened regulation. It is recommended that a web-site containing the information on the EDs and the GMOs be prepared for the journalists. In addition, a training program in relation to the EDs and the GMOs needs to be organized by the Korean Press Foundation and the Korea Food and Drug Administration to educate the journalists. A committee consisting of government officials, scientists, and NGO workers needs to be established, and it should provide framework of future policies and public relations programs.

Allergenicity assessment of novel proteins expressed in genetically modified organisms (유전자변형생물체의 알레르기성 평가와 이해)

  • Lee, Sang-Gu;Oh, Seon-Woo;Park, Soo-Yun;Park, Hyoun-Min;Kim, Eun-Ha;Jin, So-Ra;Ryu, Tae-Hun
    • Journal of Plant Biotechnology
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    • v.48 no.4
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    • pp.201-206
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    • 2021
  • To ensure the safety of developing or importing genetically modified organisms (GMOs), Korea has enacted the "LMO Act." Accordingly, the safety of using GMOs as food or feed is evaluated in accordance with the concept of "substantial equivalence" proposed by OECD. The allergenicity of GMOs is assessed as a part of their safety evaluation. The methods of allergenicity assessment have been discussed by various international organizations, such as the OECD, FAO, and WHO. The main methods used for the allergenicity assessment of proteins newly expressed in GMOs include assessment of the physicochemical stability of these proteins, evaluation of their amino acid homology with existing allergenic proteins, and serum screening. In this study, we describe guidelines and related studies for the allergenicity assessment of GM crops.

A study of Liability for LMO and Biomedicine (LMO에 대한 손해배상책임 - 바이오신약을 중심으로)

  • Moon, Sang-Hyuk
    • The Korean Society of Law and Medicine
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    • v.12 no.1
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    • pp.43-67
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    • 2011
  • Humankind history is faced with one gigantic turning point due to development of Living genetically Modified Organisms. Food production by means of LMO is on the acceleration in an effort to solve the shortage of food problems. Food is also used as alternative energy source. Use of LMO product is not only limited to food and energy, but is actively utilized in various fields of medicines. This paper is first to check out the state of biomedicine developed and associated problems from industries that use LMO, after which we made an attempt on legislative approach to find out means of relief, through examples of such laws legislated for the sufferer from the adverse effect of the biomedicine. As for the liable subject to bear the responsibility for compensatory damage in a way of relieving the victim owing to adverse effect of biomedicine, those who manufactured and sold biomedicine and who are related to the damage to the victim due to the accident and medical doctors and pharmacists who prescribe and administer the medicine in question have been looked into. Accidents involving medicines and medical supplies could take place without reason for imputation on part of the liable subjects or fault of the victim, in which case the victim can't receive damage compensation from any of both parties. When such accidents happened turn out to be no fault accidents, introduction of damage relief measures might have to be reviewed against side effects of medicine and medical supplies as no fault compensation in order for actual relief to be possible. Talking about technicality of legislation, we can suggest a method of strengthening the accountability of manufacturer for stereotypical agenda on biomedicines by newly legislating special regulation with an issue that resists claim on risks associated with the development of medicine and incorporating the same into Manufactured Product Liability Law. After all, when an accident happens associated with biomedicine, the damage will be done to the consumer. And the consumer will be exposed to fatal danger even without the time to cope with potential risks associated with medicine and medical supplies they take. Therefore, it is necessary to protect the potential victim by having the manufacturer of biomedicines bear the liability of medical risks.

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Production and Characterization of Monoclonal Antibodies Specific to PAT Protein Expressed in Genetically Modified Herbicide-Resistance Maize (제초제 내성 유전자 변형 옥수수 중 PAT단백질에 특이한 단크론성 항체의 생산과 특성 확인)

  • Kim, Sol-A;Lee, Jeong-Eun;Shim, Won-Bo;Kang, Sung-Jo;Chung, Duck-Hwa
    • Journal of Food Hygiene and Safety
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    • v.33 no.3
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    • pp.193-199
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    • 2018
  • In this study, PAT protein of genetically modified maize was prepared from the recombinant E. coli strain BL21 (DE3), and mice were immunized with the recombinant PAT protein. After cell fusion and cloning, two hybridoma cells (PATmAb-7 and PATmAb-12) were chosen since the monoclonal antibodies (Mabs) produced by them were confirmed to be specific to PAT protein in the indirect enzyme-linked immunsorbent assay (ELISA) and western blot tests. There were no cross-reactions of either Mabs to other GM proteins or to the extracts of non-GM maize. The ELISA based on the PATmAb-7 can sensitively detect 0.3 ng/g PAT protein in corn. These results indicate that the developed Mabs can be used as bio-receptors in the development of immunosensors and biosensors for the rapid and simple detection of GM corn adulterated in foods.

Quantitation of CP4 5-Enolpyruvylshikimate-3-Phosphate Synthase in Soybean by Two-Dimensional Gel Electrophoresis

  • KIM YEON-HEE;CHOI SEUNG JUN;LEE HYUN-AH;MOON TAE WHA
    • Journal of Microbiology and Biotechnology
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    • v.16 no.1
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    • pp.25-31
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    • 2006
  • Changes of CP4 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) in the glyphosate-tolerant Roundup Ready soybean were examined using purified CP4 EPSPS produced in cloned Escherichia coli as a control. CP4 EPSPS in genetically modified soybean was detected by twodimensional gel electrophoresis (2-DE) and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and electrospray ionization tandem mass spectrometry (ESI-MS/MS) with databases. CP4 EPSPS in soybean products was resolved on 2-DE by first isoelectric focusing (IEF) based on its characteristic pI of 5.1, followed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) based on its molecular mass of 47.5 kDa. We quantified various percentages of soybean CP4 EPSPS. The quantitative analysis was performed using a 2D software program on artificial gels with spots varying in Gaussian volumes. These results suggested that 2-DE image analysis could be used for quantitative detection of GM soybean, unlike Western blotting.

Food Purchasing and Quality Management Practices in School Food Service (학교급식 식재료 구매관리 및 품질관리 실태조사)

  • Kim, Gyeong-A;Gwak, Dong-Gyeong;Lee, Gyeong-Eun
    • Journal of the Korean Dietetic Association
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    • v.12 no.4
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    • pp.329-341
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    • 2006
  • The purposes of the study were to examine food purchasing management practices and to assess dietitians' awareness on food quality standards, use of food purchasing guidelines(food quality guidelines, supplier selection criteria), and organizational collaboration related to purchasing at school food service. A total of 400 dietitians working at elementary schools in Kyunggi Province were surveyed and 247 responses were returned. Excluding responses with significant missing data, 240 responses(60%) were used for data analysis. Compared to the previous reports, competitive bidding and shorter contract periods were preferred for purchasing food supplies. A dietitian, school staff, and food service staff participated in receiving and inspection together at 58.4% of the schools. An average score of the dietitians' awareness on the food quality standards was 3.28 based on a 5-point scale(1:strongly disagree, 5:strongly agree). Their awareness scores for the quality standards related to the genetically modified organism foods, organic foods, and pesticide residues were the lowest. The awareness scores increased significantly with their age(p<.001). In general, the food purchasing guidelines were used well at the schools ; the usage levels of the food quality guidelines and supplier selection criteria were significantly different by length of operation(p<.05) and dietitians' age(p<.05), respectively. The dietitians' perception score of the organizational collaboration related to purchasing was rated 3.46 and differed significantly by dietitians' educational background(p<.05). For improving food procurement management, standardized food quality and purchasing criteria need to be developed. As the demands on high quality food supplies increase for school food service, the dietitians should improve their knowledges on the food quality standards and implement effective and creative purchasing methods.

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Codex Guideline for Organically Grown Food and its Implementation of Organic Crop and Animal Production in Korea (Codex 유기식품규격 내용과 한국 유기경종과 축산의 적응 실천)

  • 손상목
    • Korean Journal of Organic Agriculture
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    • v.8 no.3
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    • pp.17-34
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    • 2000
  • This paper is aim to report the core aspects of Codex guideline for organically grown food which is finalized by FAO/WHO Codex Alimentarius Commission on May 2000 in 28th session of the Codex committee on food labelling. The chapter of animal production had discussed for a long time before it was finalized in Ottawa/canada as well as use of GMO(Genetically modified organism), manure from factory farming, animal welfare, and fodder inputs consisting of at least 85% for ruminants and 80% for non-ruminants. As the guideline for Codex set out the several things which is very difficult for Asian country, Organic farmer in Korea should pay an attention to maintain/increase the fertility and biological activity by cultivation of legumes, green manures or deep-rooting plants in an appropriate multi-annual rotation programme, and incorporation in the soil of organic material from holding producing in accordance with the guidelines. Pest, diseases and weeds should be controlled by choice of appropriate varieties, appropriate rotation, mechanical cultivation, diversified ecosystems, flame weeding, animal weeding and steam sterilization. The use of plant growth hormone, GMO and manure from industrial management system are not allowed, and closed recycling system, rotation, resistant seeds again pest and disease should be practiced in organic farming. But these are not unfortunately practiced in the country. In the conclusion it was strongly suggested to enact the Basic Standard for Korean organic agriculture which contains the core principles of Codex guidelines, and to try the importation of the most advanced theory, skills and technology from leading country in organic farming.

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Study about gene flow and stability assessment in GM rice (Oryza sativa L.)

  • Kim, Jun-Hoi;Kim, Kyung-Min
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.136-136
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    • 2017
  • In agriculture, the rice is the one of important things. Many farmers and scientists have long tried to increase the yield of rice. So many technologies have been developed these days. One type of technology has given rise to a host of concerns and questions, namely Genetically Modified Organisms (GMOs). The increasing cultivation of GM crops has raised a wide range of concerns with respect to food safety, environmental effects and socio-economic issues and now commercially planted on about 100 million hectares in some 22 developed and developing countries. The scientific evidence concerning the environmental and health impacts of GMOs is still emerging, but so far there is no conclusive information on the definitive negative impacts of GMOs on health or the environment. Nevertheless, public perceptions about GMOs in food and agriculture are divided with a tendency toward avoiding GM food and products in many developed and developing countries. Also Korea is one of that country and is not allow the GMOs now. So I studied whether these GMOs are actually dangerous for environment and whether there are differences in cultivar characteristics such as germination test with TTC tetrazolium, germination test in frozen soil and gene-flow test with glufosinate and strip-bar test. With these experiments, we evaluated the agricultural safety of GM rice and to identify and assess environmental risks.

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Current status on the development of detection methods for genetically modified plants (유전자변형식물의 검정기술 개발 현황)

  • Kim, Jae-Hwan;Kim, Young-Rok;Kim, Hae-Yeong
    • Journal of Plant Biotechnology
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    • v.38 no.2
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    • pp.143-150
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    • 2011
  • Since the first commercial GM plant, the FlavrSavr tomato, authorized in 1994, more than 140 GM plants were authorized for marketing globally. For the authorization and labelling of GM plants, the detection methods for genes introduced and proteins expressed in GM plants were developed qualitatively and quantitatively. This review presented the detection methods, conventional PCR, multiplex PCR and real-time PCR, for soybean, maize, canola and cotton as the dominant GM plants. Also, microarray assay and nanotechnology as new approaches for detection methods for GM plants were investigated.