• Advances in concrete construction
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• v.11 no.1
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• pp.1-9
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• 2021

This article discusses the issue of optimizing controller design issues, in which the artificial intelligence (AI) evolutionary bat (EB) optimization algorithm is combined with the fuzzy controller in the practical application of the building. The controller of the system design includes different sub-parts such as system initial condition parameters, EB optimal algorithm, fuzzy controller, stability analysis and sensor actuator. The advantage of the design is that for continuous systems with polytypic uncertainties, the integrated H2/H∞ robust output strategy with modified criterion is derived by asymptotically adjusting design parameters. Numerical verification of the time domain and the frequency domain shows that the novel system design provides precise prediction and control of the structural displacement response, which is necessary for the active control structure in the fuzzy model. Due to genetic algorithm (GA), we use a hierarchical conditions of the Hurwitz matrix test technique and the limits of average performance, Hierarchical Fitness Function Structure (HFFS). The dynamic fuzzy controller proposed in this paper is used to find the optimal control force required for active nonlinear control of building structures. This method has achieved successful results in closed system design from the example.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.16 no.5
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• pp.1414-1430
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• 2022

Due to the Internet of Things popularity, many agricultural data are collected by sensors automatically. The abundance of agricultural data makes precise prediction of rice yield possible. Because the climate factors have an essential effect on the rice yield, we considered the climate factors in the prediction model. Accordingly, this paper proposes a machine learning model for rice yield prediction in Taiwan, including the genetic algorithm and support vector regression model. The dataset of this study includes the meteorological data from the Central Weather Bureau and rice yield of Taiwan from 2003 to 2019. The experimental results show the performance of the proposed model is nearly 30% better than MARS, RF, ANN, and SVR models. The most important climate factors affecting the rice yield are the total sunshine hours, the number of rainfall days, and the temperature.The proposed model also offers three advantages: (a) the proposed model can be used in different geographical regions with high prediction accuracies; (b) the proposed model has a high explanatory ability because it could select the important climate factors which affect rice yield; (c) the proposed model is more suitable for predicting rice yield because it provides higher reliability and stability for predicting. The proposed model can assist the government in making sustainable agricultural policies.

• Korean Journal of Food Science and Technology
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• v.35 no.2
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• pp.266-271
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• 2003

Insoluble polyphenol antioxidants, quercetin and catechin, were stabilized through the complexation with cyclodextrin to increase heat and pH stabilities. Comparison of inclusion complex formabilities of quercetin and catechin with ${\alpha}-,\;{\beta}-$, and ${\gamma}-CDs$ revealed ${\beta}-CD$ to be the most suitable result. Optimal molar mixing ratio of ${\beta}-CD$ and quercetin or catechin for inclusion complex formation was found to be 1 : 1. Inclusion complexation was confirmed using differential scanning calorimetry. Solubility of ${\beta}-CD-antioxidant$ inclusion complexes increased compared with native antioxidants, Stability against temperature and pH of ${\beta}-CD-antioxidant$ inclusion complex analyzed revealed antioxidant activities of ${\beta}-CD-quercetin$ and catechin inclusion complexes have higher stabilization compare to raw quercetin and catechin. Peroxide value of linoleic acid dissolved in water decreased substantionally after using ${\beta}-CD-quercetin$ inclusion complex. ${\beta}-CD-antioxidant$ inclusion complex can be used effectively as a fresh-food preservative.

• Journal of Life Science
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• v.22 no.9
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• pp.1173-1179
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• 2012

The importance of genetic stability and bio-safety in the environment has recently been recognized for many (genetically modified) GM plants. This study evaluated the GM safety of transgenic rice and its environmental variance. Data on agronomic characters and principal component were collected for vitamin A-enriched GM rice and four check cultivars in a large GM field trial during 2009-2011. The cultivation environment was a large GM field and a greenhouse. In this experiment, there was no significant difference between the agronomic characters of the GM rice and those of a donor plant, 'Nagdong'. In terms of grain characteristics, the appearance and physicochemical characteristics of the GM rice and those of the donor plant were similar. However, the grain of the GM rice developed a white core and a white belly when planted in the greenhouse. The type and distribution of dominant weed species were not different in the GM rice and the 'Nagdong'. In addition, gene flow was not detected in the dominant weed species based on PCR analysis.

• International Journal of Automotive Technology
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• v.4 no.4
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• pp.181-191
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• 2003

Since the nonlinearity and uncertainties which inherently exist in vehicle system need to be considered in active suspension control law design, this paper proposes a new control strategy for active vehicle suspension systems by using a combined control scheme, i.e., respectively using a genetic algorithm (GA) based self-tuning PID controller and a fuzzy logic controller in two loops. In the control scheme, the PID controller is used to minimize vehicle body vertical acceleration, the fuzzy logic controller is to minimize pitch acceleration and meanwhile to attenuate vehicle body vertical acceleration further by tuning weighting factors. In order to improve the adaptability to the changes of plant parameters, based on the defined objectives, a genetic algorithm is introduced to tune the parameters of PID controller, the scaling factors, the gain values and the membership functions of fuzzy logic controller on-line. Taking a four degree-of-freedom nonlinear vehicle model as example, the proposed control scheme is applied and the simulations are carried out in different road disturbance input conditions. Simulation results show that the present control scheme is very effective in reducing peak values of vehicle body accelerations, especially within the most sensitive frequency range of human response, and in attenuating the excessive dynamic tire load to enhance road holding performance. The stability and adaptability are also showed even when the system is subject to severe road conditions, such as a pothole, an obstacle or a step input. Compared with conventional passive suspensions and the active vehicle suspension systems by using, e.g., linear fuzzy logic control, the combined PID and fuzzy control without parameters self-tuning, the new proposed control system with GA-based self-learning ability can improve vehicle ride comfort performance significantly and offer better system robustness.

• Journal of Microbiology and Biotechnology
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• v.23 no.5
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• pp.674-680
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• 2013

Yeast Dekkera/Brettanomyces bruxellensis is probably the most common contaminant in wineries and ethanol production processes. The considerable economic losses caused by this yeast, but also its ability to produce and tolerate high ethanol concentrations, make it an attractive subject for research with potential for industrial applications. Unfortunately, efforts to understand the biology of D. bruxellensis and facilitate its broader use in industry are hampered by the lack of adequate procedures for delivery of exogenous DNA into this organism. Here we describe the development of transformation protocols (spheroplast transformation, LiAc/PEG method, and electroporation) and report the first genetic transformation of yeast D. bruxellensis. A linear heterologous DNA fragment carrying the kanMX4 sequence was used for transformation, which allowed transformants to be selected on plates containing geneticin. We found the spheroplast transformation method using 1M sorbitol as osmotic stabilizer to be inappropriate because sorbitol strikingly decreases the plating efficiency of both D. bruxellensis spheroplast and intact cells. However, we managed to modify the LiAc/PEG transformation method and electroporation to accommodate D. bruxellensis transformation, achieving efficiencies of 0.6-16 and 10-20 transformants/${\mu}g$ DNA, respectively. The stability of the transformants ranged from 93.6% to 100%. All putative transformants were analyzed by Southern blot using the kanMX4 sequence as a hybridization probe, which confirmed that the transforming DNA fragment had integrated into the genome. The results of the molecular analysis were consistent with the expected illegitimate integration of a heterologous transforming fragment.

• Reproductive and Developmental Biology
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• v.37 no.1
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• pp.1-8
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• 2013

In order to investigate genetic stability and gene expression profile after cloning procedure, two groups of cloned pigs were used for swine leukocyte antigen (SLA) gene nucleotide alteration and microarray analyses. Each group was consist of cloned pigs derived from same cell line (n=3 and 4, respectively). Six SLA loci were analyzed for cDNA sequences and protein translations. In total, 16 SLA alleles were identified and there were no evidence of SLA nucleotide alteration. All SLA sequences and protein translations were identical among the each pig in the same group. On the other hand, microarray assay was performed for profiling gene expression of the cloned pigs. In total, 43,603 genes were analyzed and 2,150~4,300 reliably hybridized spots on the each chip were selected for further analysis. Even though the cloned pigs in the same group had identical genetic background, 18.6~47.3% of analyzed genes were differentially expressed in between each cloned pigs. Furthermore, on gene clustering analysis, some cloned pigs showed abnormal physiological phenotypes such as inflammation, cancer or cardiomyopathy. We assumed that individual environmental adaption, sociality and rank in the pen might have induced these different phenotypes. In conclusion, the results of the present study indicate that SLA locus genes appear to be stable following SCNT. However, gene expressions and phenotypes between cloned pigs derived from the same cell line were not identical even under the same rearing conditions.

• Journal of IKEEE
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• v.1 no.1 s.1
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• pp.19-30
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• 1997

In this paper we design the optimal satellite-tracking antenna $H{\infty}$ control system using genetic algorithms. To do this, we give gain and dynamics parameters to the weighting functions and apply genetic algorithms with reference model to the optimal determination of weighting functions and design parameter ${\gamma}$ that are given by Glover-Doyle algorithm which can design $H{\infty}$ controller in the state space. These weighting functions and design parameter ${\gamma}$ are optimized simultaneously in the search domain guaranteeing the robust stability of closed-loop system. The effectiveness of this satellite-tracking antenna $H{\infty}$ control system is verified by computer simulation.

• Structural Engineering and Mechanics
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• v.65 no.3
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• pp.303-314
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• 2018

Shear walls are a typical member under a complex stress state and have complicated mechanical properties and failure modes. The separated-elements model Genetic Evolutionary Structural Optimization (GESO), which is a combination of an elastic-plastic stress method and an optimization method, has been introduced in the literature for designing such members. Although the separated-elements model GESO method is well recognized due to its stability, feasibility, and economy, its adequacy has not been experimentally verified. This paper seeks to validate the adequacy of the separated-elements model GESO method against experimental data and demonstrate its feasibility and advantages over the traditional elastic stress method. Two types of reinforced concrete shear wall specimens, which had the location of an opening in the middle bottom and the center region, respectively, were utilized for this study. For each type, two specimens were designed using the separated-elements model GESO method and elastic stress method, respectively. All specimens were subjected to a constant vertical load and an incremental lateral load until failure. Test results indicated that the ultimate bearing capacity, failure modes, and main crack types of the shear walls designed using the two methods were similar, but the ductility indexes including the stiffness degradation, deformability, reinforcement yielding, and crack development of the specimens designed using the separated-elements model GESO method were superior to those using the elastic stress method. Additionally, the shear walls designed using the separated-elements model GESO method, had a reinforcement layout which could closely resist the actual critical stress, and thus a reduced amount of steel bars were required for such shear walls.

• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.3
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• pp.174-180
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• 2000

Cyclodextrin glucanotransferase (CGTasa) from Thermoanaerobacter sp. was adsorbed on the ion exchange resin Amberlite IRA-900. The optimum conditions for the immobilization of the CGTase were pH6.0 and 600 U CGTase/g resin, and the maximum yield of immobilization was around 63% on the basis of amount ratio of the adsorbed enzyme to intial amount in the solution. Immobilixation of CGTase shifted the optimum temperature for the enzyme to peoduce transglycosylated xylitol from 7$0^{\circ}C$ to 9$0^{\circ}C$ and improved the thermal stability of immobilized CGTase, especially after the addition of soluble starch and calcium ions. Transglycosylated xylitol was continuoncly produced using immobilized CGTase in the column type packed bed reactor, and the operating conditions for maximum yield were 10%(w/v) dextrin (13 of the dextrose equivalent) as the glycosyl donor, 10%(w/v) dextrin (13 of the dextrose equivalent) as the glycosyl donor, 10%(w/v) xylitor as the glycosyl acceptor, 20mL/h of medium fiow rate, and 6$0^{\circ}C$. The maximum yield of transglycosylated xylitol and productivity were 25% and 7.82 g.L-1.h-1, respectively. The half-life of the immobilized CGTase in a column type packed bed reactor was longer than 30 days.