• 제목/요약/키워드: Genetic stability

검색결과 393건 처리시간 0.024초

Transformation of an Alkalin Protease Overproducer, Vibrio metschnikovii Strain RH530, and Improvement of Plasmid Stability by the par Locus

  • Chung, So-Sun;Shin, Yong-Uk;Kim, Hee-Jin;JIn, Chee-Hong;Lee, Hyune-Hwan
    • Journal of Microbiology and Biotechnology
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    • 제11권2호
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    • pp.222-228
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    • 2001
  • Vibrio metschnikovii strain RH530 is a non-pathogenic, industrially-important alkaline protease producer which has been isolated from wastewater. In this paper, we report on the transformation of this strain by using the method of electroporation. A field strength of $7.5\;kVcm^{-1}$ and $25\;{\mu}F$, and using a 0.2-cm cuvette, appeared to be the optimal conditions for electroporation of the cells with the recombinant pSBCm plasmid carrying the vapK alkaline protease gene and the ColE1 replicon. Cells were subjected to osmotic shock in order to remove extracelluar DNase, and adding 200 mM of sucrose to electroporation buffer cells showed an increased transformation efficiency. Maximum efficiency of transformation was obtained at an early exponential growth phase. Using all of the conditions mentioned above, we routinely obtained a transformation efficiency of more than $10^4{({\mu}g\;plasmid\;DNA)}^{-1}$. The stability of the plasmid pSBCm in V. metschnikovii RH530 was 25% after 18h of growth (27 generations) in the medium without antibiotic selection. The insertion of the par locus to the pSBCm increased the stability of the plasmid up to 42% without selective pressure. The increase in plasmid stability was accompanied by the increase in the productivity of alkaline protease in the recombinant V. metschnikovii strain RH530. Determining optimal conditions for the transformation of the industrially-important, nonpathogenic Vibrio strain, and the improvement of plasmid stability by introducing the par locus into the high copy number plasmid vector, will allow the development of procedures involved in the genetic manipulation of this strain, particularly for its use in the production of industrial enzymes such as alkaline protease.

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효모균에서의 Plasmid 번식체계와 혼성유전자 발현 (Plasmid Propagation and Heterologous Gene Expression in Recombinant Yeast)

  • 홍억기
    • KSBB Journal
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    • 제8권2호
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    • pp.133-142
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    • 1993
  • 효모균에서의 유전자 재조합에 의한 단백절 생산에 미치는 유전학적, 환경적인 요인의 영향을 연구하였. Plasmid 안정도와 개수는 $REP^+$ 체계 에서 대단히 높은 반면, rep 체계에서는 매우 낮았다. $2{\mu}m$ circle plasmid genome을 포함하는 plasmid의 경우에 있어서. $[cir^o]$ 형 세포에서의 plasmid 안정도와 개수가 $[cir^+]$형 세포에서보다 높기때문에 $[cir^+o]$형 세포가 더 선호되는 세포였다. 유전자 발현은 plasmid 개수와 안정도에 좌우 되었다. 촉진제의 양이 유전자 발현에 매우 중요 한 역할을 했다. 유전자 발현의 촉진에 필요한 g떠actose의 농도는 0.8% 이 변 충분했다. 높은 안정 도와 개수를 갖는 plasmid의 경우 촉진속도는 매우 빨랐다. Galactose가 배양의 시작부분부터 첨가 될 때가 mid-exponential ph잃e에 첨가될 때보다 유전자 발현의 극대점에 이르는 시간이 걸었다. 상대적 촉진제의 양이 증가함에 따라 glucc잉e억제 현상은 감소되었다.

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천연색소로서 한국산 유색미 안토시아닌의 안정성 연구 (Physicochemical Stability of Anthocyanins from a Korean Pigmented Rice Variety as Natural Food Colorants)

  • 윤주미;조만호;한태룡;백영숙;윤혜현
    • 한국식품과학회지
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    • 제29권2호
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    • pp.211-217
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    • 1997
  • 유용한 천연색소원으로서, 한국산 유색미에서 안토시아닌을 분리하여 pH, 온도, 금속이온, 당 및 유기산에 대한 안정성을 조사하였다. 몇가지 pH 조건에서 21일간 저장하였을 때, pH 2.0와 pH 3.0에서는 안토시아닌의 반감기가 약 36일과 17일로 나타났으며 중성과 염기성 pH에서는 하루 이내에 거의 분해되는 것으로 나타나 산성조건의 식품에서는 안정하게 이용될 수 있음을 시사하였다. 온도의 영향에 대한 실험 결과, 반감기가 $95^{\circ}C$에서 7.4시간(pH 3.0)과 0.17시간(pH 9.0), $75^{\circ}C$에서 23.6시간(pH 3.0), 0.57시간(pH 9.0), $50^{\circ}C$에서는 96.3시간(pH 3.0), 1.7시간(pH 9.0)으로 산성조건에서는 열에 대해서도 비교적 안정하였다. 금속이온 첨가에 따른 농색화 효과는 1가, 2가, 3가로 갈수록 높아졌으며 농도가 10 mM보다 100 mM 첨가시 더 높은 흡광도를 보였고, 2가이상의 금속이온들은 대부분 높은 안정화 효과를 보였다. 당 농도의 경우 단당류는 0.1 M, 이당류는 0.15 M 일때가 가장 높은 농색화 효과를 보여주었으나, 저장하는 동안 fructose가 안토시아닌 분해를 가장 촉진하는 것으로 나타났고, 당 첨가군은 모두 대조군보다 낮은 색소 잔존량을 보여주었다. 유기산 용액의 pH를 3.0으로 고정하여 몇가지 유기산의 영향을 조사한 결과, citric acid가 가장 농색화 및 안정화 효과가 있었으며, tartaric acid 첨가군은 대조군보다 불안정하였다. 20,000 lux의 빛에 노출시켜 빛이 미치는 영향을 조사한 결과, 유색미 안토시아닌의 반감기가 약 14시간으로 나타나 빛에 매우 민감함을 알 수 있었다. 함량이 많은 유리아미노산으로는 glutamic Acid, lysine, arginine, aspartic acid, histidine, leucine, alanine 등으로서 일반토하젓에서의 조성과 상이하였다. 양념토하젓은 glutamic acid를 제외한 대부분의 유리아미노산이 일반토하젓에서보다 낮았다. 일반토하젓과 양념토하젓의 핵산관련물질함량은 각각 $2.64{\sim}4.82\;{\mu}mol$$1.08{\sim}1.93\;{\mu}mol$로서 제품에 따른 차이가 많았다. 일반토하젓에서는 homarine이 $18{\sim}86\;mg$ 검출되었으나 바다새우젓의 30.7%였고 trigonelline은 비슷한 수준이었다. 양념토하젓은 homarine이 흔적${\sim}39\;mg$, trigonelline $0{\sim}5\;mg$으로서 일반토하젓보다 낮았다. 일반토하젓과 양념토하젓의 TMAO와 TMA 함량은 미량으로서 바다새우젓의 20.9%와 27.7% 수준이었다. 일반토하젓과 양념토하젓에서 분석된 각성분들에 대한 질소분포는 모두 유리아미노산이 가장 중요한 함질소성분으로서 평균 65.7%와 60.8%였고, 다음은 oligopeptide류로서 17.8%와 16.6%였다. 핵산관련물질은 3.9%와 2.2%, betaine류는 1.1%와 0.4%, TMAO와 TMA는 1.2%와 1.8%, 그리고 creatine은 0.4%와 9.6%로서 엑스분질소의 회수율은 90.3%와 91.2%였다. 이상에서 분석된 토하젓의 엑스분질소와 함질소 엑스성분들을 상관분석한 결과 엑스분 질소, betaine 총량, 유리아미노산 총량 및 유리아미노산의 일종인 phenylalanine 이 토하젓의 품질지표로 활용가능성이

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과도안정도 에너지 마진 향상을 위한 TCSC 적정치의 실시간 산정 (Real-Time Estimation of TCSC Quantity for Improvement of Transient Stability Energy Margin)

  • 김수남;유석구
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2000년도 하계학술대회 논문집 A
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    • pp.242-244
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    • 2000
  • This paper presents a method for real-time estimation of TCSC quantity in order to enhance the power system transient stability energy margin using fuzzy neural network in multi-machine system. This paper has two parts, the first part is to estimate the energy margin. To set critical energy, we use the potential energy boundary surface(PEBS) method which one of the transient energy function(TEF) method. And the second is to determine the TCSC quantify and the line to be injected. In order to make training data in this step, we use genetic algorithm. The proposed method is applied to 6-bus, 7-line, 4-machine model system to show its effectiveness.

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전력계통 안정도 향상을 위한 GA적용 LQ-PSS 설계 (Design of LQ-PSS for Power System Stability Enhancement using GA)

  • 정영민;이석오;김형수;박준호;황기현;이화석
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2001년도 하계학술대회 논문집 A
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    • pp.128-130
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    • 2001
  • This paper proposes the design of LQ-PSS(linear quadratic power system stabilizer) for improving power system stability using genetic algorithm(GA). We are tuned weighting matriecs of LQ-PSS using GA. To evalute the usefulness of the proposed method. we performed the nonlinear simulation on a single machine infinite system. As results of the simulation. the proposed method shows the better control performance than CPSS(conventional power system stabilizer) in trems of settling time and damping effects.

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전력시스템의 안정도 향상을 위한 GA-퍼지 전 보상기 설계 (Design of GA-Fuzzy Precompensator for Enhancement of Power System Stability)

  • 정문규;김상효;정형환;이동철
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2001년도 하계학술대회 논문집 A
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    • pp.137-139
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    • 2001
  • In this paper, we design a GA-fuzzy precompensator for enhancement of power system stability. Here, a fuzzy precompensator is designed as a fuzzy logic-based precompensation approach for Power System Stabilizer(PSS). This scheme is easily implemented simply by adding a fuzzy precompensator to an existing PSS. And we optimize the fuzzy precompensator with a genetic algorithm for complements the demerit such as the difficulty of the component selection of fuzzy controller, name1y, scaling factor, membership function and control rules. Simulation results show that the proposed control technique is superior to a conventional PSS in dynamic responses over the wide range of operating conditions and is convinced robustness and reliableness in view of structure.

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UPFC를 이용한 과도안정도 에너지마진 향상 (Improvement of Transient Stability Energy Margin by using UPFC)

  • 이승걸;김수남;유석구
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2001년도 하계학술대회 논문집 A
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    • pp.152-154
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    • 2001
  • This paper presents a method for determination of UPFC control quantity in order to enhance the power system transient stability energy margin using Genetic Algorithms in multi-machine system. We use the minimization of energy margin as the object function in GA. To set critical energy, we use the potential energy boundary surface(PEBS) method. PEBS is one of the transient energy function(TEF) method. And we used the series voltage compensator as the UPFC model. The proposed method is applied to 6-bus, 7-line, 4-machine model system to show its effectiveness.

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다기계통의 과도 안정도 향상 (An Improvement of Transient Stability of Multi-machine Power System)

  • 김수남;유석구
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 1997년도 하계학술대회 논문집 D
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    • pp.911-913
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    • 1997
  • This paper presents a method for optima] placement of series capacitors in order to improve the power system transient stability, using genetic algorithms. For the formulation, this paper considers the objective function which is the energy margin as the difference between transient energy and critical energy. The most important factor in determining an accurate critical energy is the controlling unstable equilibrium point (UEP). This paper proposes the controlling UEP methods, concurrently with the DFP(Davidon-Fletcher-Powell) method, which enables the enhancement of multi-machine analysis. The proposed method is applied to 6-bus, 7-line, 4-machine model system to show its effectiveness in determining the locations to install series capacitors and the it's size to be installed in system, simultaneously.

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Dynamics and GA-Based Stable Control for a Class of Underactuated Mechanical Systems

  • Liu, Diantong;Guo, Weiping;Yi, Jianqiang
    • International Journal of Control, Automation, and Systems
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    • 제6권1호
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    • pp.35-43
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    • 2008
  • The control of underactuated mechanical system is very complex for the loss of its control inputs. The model of underactuated mechanical systems in a potential field is built with Lagrangian method and its structural properties are analyzed in detail. A genetic algorithm (GA)based stable control approach is proposed for the class of under actuated mechanical systems. The Lyapunov stability theory and system properties are utilized to guarantee the system stability to its equilibrium. The real-valued GA is used to adjust the controller parameters to improve the system performance. This approach is applied to the underactuated double-pendulum-type overhead crane and the simulation results illustrate the complex system dynamics and the validity of the proposed control algorithm.

과도안정도 향상을 위한 직렬콘덴서의 최적화 방안 (An Optimization Method of Series Condenser for Improvement of Transient Stability)

  • 유석구;문병서;김규호
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 1996년도 하계학술대회 논문집 B
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    • pp.890-892
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    • 1996
  • This paper presents a method for optimal placement of series condenser in order to improve the power system transient stability using genetic algorithms(GAs). In applying GAs, this approach utilizes two kinds of strings, one is coded by a binary finite-length for the selection of lines to install series condenser, the other is coded by a real value for the determination of injected condenser capacitance. For the formulation. this paper considers multi-objective function which is the critical energy as decelerating energy in power systems and the total injected condenser capacitance. The proposed method is applied to 9-bus, 18-line, 3-machine model system to show its effectiveness in determining the locations to install series condenser and the series condenser capacitance to be injected, simultaneously.

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