• 한국환경성돌연변이발암원학회지
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• 제22권4호
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• pp.215-222
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• 2002

The detection of many synthetic chemicals used in industry that may pose a genetic hazard in our environment is of great concern at present. Since these substances are not limited to the original products, and enter the environment, they have become widespread environmental pollutants, thus leading to a variety of chemicals that possibly threaten the public health. In this respect, to regulate and to evaluate the chemical hazard will be important to environment and human health. The clastogenicity of 17 synthetic chemicals was evaluated in Chinese hamster lung fibroblast cells in vitro. Two most cytotoxic chemicals, dodecyl methacrylate (CAS No. 142-90-5) and 2-ethylhexyl methacrylate (CAS No. 688-84-6), among 17 chemicals tested revealed no clastogenicity in the range of 0.0165-0.066 $\mu\textrm{g}$/$m\ell$ and 0.006-0.024 $\mu\textrm{g}$/$m\ell$ both in the presence and absence of metabolic activation system, respectively. All 17 chemicals revealed no significant induction of chromosomal aberration both in the presence and absence of metabolic activation system in this assay. From the results of chromosomal aberration assay with 17 synthetic chemicals in Chinese hamster lung cells in vitro, we did not observed positive clastogenic results in this study.

• Current Research on Agriculture and Life Sciences
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• 제23권
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• pp.43-51
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• 2005

멕시코와 네팔에서 수집한 고추 유전자원 50점과 이병성 및 저항성 대조품종에 Stemphylium solani와 S. lycopersici 의 혼합접종으로 흰별무늬병(gray leaf spot)에 대한 저항성을 검정하고, 살균제를 살포하여 회복시킨 다음 다시 Xanthomonas campestris pv. vesicatoria를 접종하여 더뎅이병에 대한 저항성을 검정하였다 더뎅이병에는 KC866, KC872, KC902, KC905이 이미 알려진 KC43, KC47, KC220, KC319, KC320, KC380 등과 함께 저항성으로 나타났다. 더뎅이병, bacterial spot)에는 KC897이 이미 저항성으로 보고된 KC177(PI163192)보다 발병이 적어 가장 우수한 것으로 나타났으며 이어서 네팔에서 도입된 KC889, KC896, KC898이 저항성으로 나타났다.

• 한국미생물·생명공학회지
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• 제37권3호
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• pp.213-218
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• 2009

The goal of this study was to develop new functional pear wine using six Asian pears (Pyrus pyrifolia, Nakai), namely Wonhwang, Niitaka, Whangkeumbae, Whasan, Gamcheonbae and Chuwhangbae. To select optimal yeast and pear, we investigated the physicochemical properties of the pear wines from fermentation of musts of six pear cultivars at $25^{\circ}C$ for 7 days by several yeasts. $11.2%{\sim}12.4%$ of ethanol from musts of 'Wonhwang', 'Whangkeumbae' and 'Whasan' were produced by Saccharomyces cerevisiae K-7 and 12.8% of ethanol was also produced from 'Niitaka' by commercial Saccharomyces cerevisiae C-2. 9.9% and 11.4% of ethanol were produced from musts of 'Gamcheonbae' and 'Chuwhangbae' by Saccharomyces cerevisiae KCTC 7904, respectively. Among several pear wines, Niitaka pear wine showed the best acceptability in the sensory evaluation, and Niitaka pear wine and Whangkeumbae pear wine showed 31.1% and 27.8% of antihypertensive angiotensin I-converting enzyme(ACE) inhibitory activity, respectively. However, the other functionalities were not detected or very low. Furthermore, Niitaka-strawberry mixed fermentation wine was showed the excellent acceptability and high antihypertensive ACE inhibitory activity of 64.9%.

• Clinical and Experimental Pediatrics
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• 제55권2호
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• pp.48-53
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• 2012

Purpose: Gaucher disease is caused by a ${\beta}$-glucocerebrosidase (GBA) deficiency. The aim of this study is to investigate the clinical and genetic characteristics according to subtypes of Gaucher disease in the Korean population. Methods: Clinical findings at diagnosis, $GBA$ mutations, and clinical courses were reviewed in 20 patients diagnosed with Gaucher disease. Results: Eleven patients were diagnosed with non-neuronopathic type, 2 with acute neuronopathic type, and 7 with chronic neuronopathic type. Most patients presented with hepatosplenomegaly, thrombocytopenia, and short stature. In the neuronopathic group, variable neurological features, such as seizure, tremor, gaze palsy, and hypotonia, were noted at age $8.7{\pm}4.3$ years. B cell lymphoma, protein-losing enteropathy, and hydrops fetalis were the atypical manifestations. Biomarkers, including chitotriosidase, acid phosphatase, and angiotensin-converting enzyme, increased at the initial evaluation and subsequently decreased with enzyme replacement treatment (ERT). The clinical findings, including hepatosplenomegaly, thrombocytopenia, and skeletal findings, improved following ERT, except for the neurological manifestations. L444P was the most common mutation in our cohort. One novel mutation, R277C, was found. Conclusion: Although the clinical outcome for Gaucher disease improved remarkably following ERT, the outcome differed according to subtype. Considering the high proportion of the neuronopathic form in the Korean population, new therapeutic strategies targeting the central nervous system are needed, with the development of a new scoring system and biomarkers representing clinical courses in a more comprehensive manner.

• 산업경영시스템학회지
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• 제40권1호
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• pp.95-104
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• 2017

This paper deals with solution methods for discrete and multi-valued optimization problems. The objective function of the problem incorporates noise effects generated in case that fitness evaluation is accomplished by computer based experiments such as Monte Carlo simulation or discrete event simulation. Meta heuristics including Genetic Algorithm (GA) and Discrete Particle Swarm Optimization (DPSO) can be used to solve these simulation based multi-valued optimization problems. In applying these population based meta heuristics to simulation based optimization problem, samples size to estimate the expected fitness value of a solution and population (particle) size in a generation (step) should be carefully determined to obtain reliable solutions. Under realistic environment with restriction on available computation time, there exists trade-off between these values. In this paper, the effects of sample and population sizes are analyzed under well-known multi-modal and multi-dimensional test functions with randomly generated noise effects. From the experimental results, it is shown that the performance of DPSO is superior to that of GA. While appropriate determination of population sizes is more important than sample size in GA, appropriate determination of sample size is more important than particle size in DPSO. Especially in DPSO, the solution quality under increasing sample sizes with steps is inferior to constant or decreasing sample sizes with steps. Furthermore, the performance of DPSO is improved when OCBA (Optimal Computing Budget Allocation) is incorporated in selecting the best particle in each step. In applying OCBA in DPSO, smaller value of incremental sample size is preferred to obtain better solutions.

• 수산경영론집
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• 제40권3호
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• pp.69-88
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• 2009

This research was as part of restocking project conducted until 1986. The necessity of its economic and scientific verification on restocking project increased. The economic analysis of this project was intended to measure its effectiveness of rockfish restocking throughout certain water areas. The rockfish restocking in 2008 was carried two regional governments of Gyeongnam and Busan. 3-year average annualized performance is 3.76 million rockfish restocked and 940 million won invested. As Gyeongnam restocked 3.62 million rockfish and invested 890 million won, most of rockfish stocking accomplished in Gyeongnam. The public shipment was the 3-year average amount was about 400 tons. Each Suhyup shipment as follows; TongYong 66.6 tons, Namhae 23.6 tons, Samcheonpo 17.2 tons; Yeosu 13.3 tons, Geoje 4.7 tons, Goseong 3.1 tons, Hadong 1.0 tons. The private shipment was assessed by the interview of relevant market participations. The high percent areas of public ship were Nemaha, Goseong 90% and Geoje 80% or more. Hadong and Samcheonpo were about 50%. TongYong was 10% and the lowest. The private shipment was calculated using the percentage of private shipment surveyed. The total amount of private shipment was 4.8 billion, where 4.5 billion was TongYong, where most of private shipment is being made. The positive economic benefit occurred when the percent of restocked is over 15% in the overall catch. The percentage of restocked investigated with genetic test was 58.2%. With 58.2% ratio of restocked, there was 3.8 times economic benefit according to approximately 3.6 billion won. Economic effects were highly significant. As catch increased according restocking, joint market commission increased. Joint market commission was calculated as total restocked shipment multiplied by the ratio of commission, the calculated commissions were as follows; Hadong 5.0%, Geoje 4.8%, Goseong 4.8%, Namhae 4.8%, Samcheonpo 4.3%, Yeosu 4.3%, TongYong 4.0%. According to calculation results, annual commission fee of ￦60,000,000 was raised during 2006~2008. The fishing catch bulk for entertainment significantly increased in sea area carried with mass restock.

• BMB Reports
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• 제40권3호
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• pp.448-452
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• 2007

We examined the contribution of CYP2C9 and CYP2C19 genotypes and drug interactions to the phenytoin metabolism among 97 Korean epileptic patients to determine if pharmacogenetic testing could be utilized in routine clinical practice. The CYP2C9 polymorphism is a wellknown major genetic factor responsible for phenytoin metabolism. The CYP219 polymorphism, with a high incidence of variant alleles, has a minor influence on phenytoin treated Koran patients. Using a multiple regression model for evaluation of the CYP2C9 and CYP2C19 genotypes, together with other non-genetic variables, we explained 39.6% of the variance in serum phenytoin levels. Incorporation of genotyping for CYP2C9 and CYP2C19 into a clinical practice may be of some help in the determination of phenytoin dosage. However, because concurrent drug treatment is common in patients taking phenytoin and many environmental factors are likely to play a role in drug metabolism, these factors may overwhelm the relevance of CYP polymorphisms in the clinical setting. Further investigations with an approach to dose assessment that includes comprehensive interpretation of both pharmacogenetic and pharmacokinetic data along with understanding of the mechanism of drug interactions in dosage adjustment is warranted.

• Asian-Australasian Journal of Animal Sciences
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• 제15권1호
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• pp.11-15
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• 2002

The present investigation was undertaken to study the effects of non-genetic factors and association among replacement and lifetime production traits. The data on 542 Holstein Friesian cows maintained during 1975-98 at State Cattle Breeding Project, Sector III, Hisar, were utilized. The average sex-ratio, abnormal births, mortality, culling and replacement rates on total calf born and total female calf born basis were 51.62, 8.50, 17.52, 31.05, 22.78 and 51.41 per cent, respectively. The study revealed that a minimum of 4 to 5 progenies are required per cow over its lifetime to replace itself. It indicated that each cow should produce a minimum of 2 female calves during its life so as to replace herself before being lost. The least-squares means for productive herd life, longevity and lifetime production were $1439.32{\pm}87.64$ and $2419.18{\pm}8.25$ days and $11317.95{\pm}913.15kg$, respectively. The heritability estimates for all replacement traits were very low indicating that sire selection may bring no desirable change in these traits. Heritability estimates were $0.178{\pm}0.157$, $0.288{\pm}0.184$ and $0.096{\pm}0.195$ for corresponding lifetime production traits. Breeding values and ranking of sires were generated for replacement and lifetime production traits to estimate the rank correlations between these traits. Moderate desirable rank correlations were obtained between replacement rate and lifetime production traits indicating that sires proven on the basis of milk production are also expected to have better replacement rate.

• 한국해양공학회지
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• 제29권5호
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• pp.366-372
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• 2015

Ocean energy is one of the most promising renewable energy resources. In particular, South Korea is one of the countries where it is economically and technically feasible to develop tidal current power plants to use tidal current energy. In this study, based on the design code for HARP_Opt (Horizontal axis rotor performance optimizer) developed by NREL (National Renewable Energy Laboratory) in the United States, and applying the BEMT (Blade element momentum theory) and GA (Genetic algorithm), the optimal shape design and performance evaluation of the horizontal axis rotor for a 200-kW-class tidal current turbine were performed using different numbers of blades (two or three) and a pitch control method (variable pitch or fixed pitch). As a result, the VSFP (Variable Speed Fixed Pitch) turbine with three blades showed the best performance. However, the performances of four different cases did not show significant differences. Hence, it is necessary when selecting the final design to consider the structural integrity related to the fatigue, along with the economic feasibility of manufacturing the blades.

• Asian Pacific Journal of Cancer Prevention
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• 제16권12호
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• pp.4927-4935
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• 2015

Northeastern India is a major nasopharyngeal carcinoma (NPC) high risk-area although the rest of the country has very low incidence. A case-control study of 105 NPC cases and 115 controls was conducted to identify the potential risk factors for NPC development in this region. Information was collected by interviewer about socio-demographic characteristics, cigarette smoking, alcohol consumption, dietary history, occupational history, and a family history of cancer. Epstein-Barr viral load was assayed from the blood DNA by real time PCR. Associations between GSTs genotypes, cytochrome P450 family including CYP1A1, CYP2E1 and CYP2A6 polymorphisms and susceptibility to relationship between the diseases were studied using PCR-RFLP assay. Results indicate that Epstein-Barr virus load was significantly higher in patients compared to controls (p<0.0001). Furthermore, concentration of blood EBV-DNA was significantly higher in advanced stage disease (Stage III and IV) than in early stage disease (Stage I and II) (p<0.05). Presence of CYP2A6 variants that reduced the enzyme activity was significantly less frequent in cases than controls. Smoked meat consumption, exposure to smoke, living in poorly ventilated house and alcohol consumption were associated with NPC development among the population of Northeastern India. Thus, overall our study revealed that EBV viral load and genetic polymorphism of CYP2A6 along with living practices which include smoked meat consumption, exposure to smoke, living in poorly ventilated houses and alcohol consumption are the potential risk factors of NPC in north eastern region of India. Understanding of the risk factors and their role in the etiology of NPC are helpful forpreventive measures and screening.