• 한국가금학회지
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• 제45권2호
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• pp.109-118
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• 2018

현대양계산업은 육종, 영양, 관리기술의 향상에 힘입어 산란계의 생산성과 육계의 성장성이 크게 향상되었다. 이러한 닭의 생산성 향상을 뒷받침해 주기 위해서는 이들의 대사적 요구를 충족시킬 수 있는 건강한 간의 기능이 필요하다. 가금에서 지방산의 대부분은 간에서 생합성되며, 이는 간의 지방대사가 닭의 생육 및 산란능력 등에 매우 중요하다는 것을 의미한다. 간에서의 지방합성이 지방의 대사능력과 분비를 초과하게 되면 중성지방은 간에 축적되어 지방간(fatty liver)을 유발하기도 한다. 산란계의 경우 산란을 시작하게 되면 혈액 내 유리지방산, 지질 및 인지질 등이 급격히 증가한다. 산란계에서 산란율이 높은 닭은 난황합성이 활발하게 일어나기 때문에 지방간을 가지는 경우가 많으며, 심한 경우 간의 손상(liver-hemorrhage)에 의해 폐사로 이어질 수 있다. 따라서 닭의 간에서 지방합성과 합성된 지방의 분비와 이용은 건강한 닭의 성장과 생산성 향상에 주요한 열쇠로 판단되어 최근까지 연구된 닭의 지방대사와 지방간 억제 혹은 예방 등에 대하여 기술하였다.

• Clinical and Experimental Pediatrics
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• 제54권1호
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• pp.36-39
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• 2011

Bartter syndrome (BS) is a clinically and genetically heterogeneous inherited renal tubular disorder characterized by renal salt wasting, hypokalemic metabolic alkalosis and normotensive hyperreninemic hyperaldosteronism. There have been several case reports of BS complicated by focal segmental glomerulosclerosis (FSGS). Here, we have reported the case of a BS patient who developed FSGS and subsequent end-stage renal disease (ESRD) and provided a brief literature review. The patient presented with classic BS at 3 months of age and developed proteinuria at 7 years. Renal biopsy performed at 11 years of age revealed a FSGS perihilar variant. Hemodialysis was initiated at 11 years of age, and kidney transplantation was performed at 16 years of age. The post-transplantation course has been uneventful for more than 3 years with complete disappearance of BS without the recurrence of FSGS. Genetic study revealed a homozygous p.Trp(TGG)610Stop(TGA) mutation in the CLCNKB gene. In summary, BS may be complicated by secondary FSGS due to the adaptive response to chronic salt-losing nephropathy, and FSGS may progress to ESRD in some patients. Renal transplantation in patients with BS and ESRD results in complete remission of BS.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2007년도 Proceedings of ISRS 2007
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• pp.438-441
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• 2007

Recently, due to molecular biology and engineering technology, DNA microarray makes people watch thousands of genes and the state of variation from the tissue samples of living body. With DNA Microarray, it is possible to construct a genetic group that has similar expression patterns and grasp the progress and variation of gene. This paper practices Cluster Analysis which purposes the discovery of biological subgroup or class by using gene expression information. Hence, the purpose of this paper is to predict a new class which is unknown, open leukaemia data are used for the experiment, and MCL (Markov CLustering) algorithm is applied as an analysis method. The MCL algorithm is based on probability and graph flow theory. MCL simulates random walks on a graph using Markov matrices to determine the transition probabilities among nodes of the graph. If you look at closely to the method, first, MCL algorithm should be applied after getting the distance by using Euclidean distance, then inflation and diagonal factors which are tuning modulus should be tuned, and finally the threshold using the average of each column should be gotten to distinguish one class from another class. Our method has improved the accuracy through using the threshold, namely the average of each column. Our experimental result shows about 70% of accuracy in average compared to the class that is known before. Also, for the comparison evaluation to other algorithm, the proposed method compared to and analyzed SOM (Self-Organizing Map) clustering algorithm which is divided into neural network and hierarchical clustering. The method shows the better result when compared to hierarchical clustering. In further study, it should be studied whether there will be a similar result when the parameter of inflation gotten from our experiment is applied to other gene expression data. We are also trying to make a systematic method to improve the accuracy by regulating the factors mentioned above.

• Archives of Plastic Surgery
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• 제32권6호
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• pp.689-698
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• 2005

Many studies for verifying angiogenesis have been in progress, especially in the field of abnormal vascular proliferation to explain the pathogenesis and to develop a treatment of several diseases. In our previous experiments, endothelial cell proliferations were induced by DMH stimulation in vitro, and the 177 factors(142 up-regulated and 35 down-regulated factors) were identified. Among the up-regulated factors, 9 substances (EFEMP1, CTGF, CYR61, $ITG{\beta}1$, FHL2, SERPINE1, MYC, PTTG1 and MSH6) were selected, which were related to cell proliferation and showed high signal intensities. The RNA was isolated from HUVECs at the time of 0, 6, 12, 24 hours after the DMH treatment, and RNA of control group HUVECs was also isolated. Genetic information of selected molecules was used to make primer for each, and RT-PCR was performed to analyze both groups. In control and treatment groups, each substance presented variety of manifestation degree according to time differences. EFEMP1, CTGF, CYR61, $ITG{\beta}1$, FHL2 and MYC were related to abnormal vascular proliferation steadily and SERPINE1, PTTG1 and MSH6 were related secondarily. CTGF was related to both normal and abnormal proliferation, but it played a more significant role in abnormal proliferation from earlier stage. EFEMP1, CYR61, $ITG{\beta}1$, FHL2 and MYC were similar to CTGF, although the relation appeared lately. Further study should be performed to analyze the expressions and the interactions of growth factors, which could be utilized in the new therapeutic development.

• 대한한의학방제학회지
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• 제18권2호
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• pp.267-277
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• 2010

Objective : Mitochondrial myopathy, encephalopathy, lactic acidosis, and stroke like episodes (MELAS) syndrome is a progressive neurodegenerative disorder. The typical presentation of patients with MELAS syndrome includes features such as mitochondrial encephalomyopathy, lactic acidosis, and stroke like episodes. Other features, such as seizures, diabetes mellitus, hearing loss, cardiac disease, short stature, endocrinopathies, exercise intolerance, and neuropsychiatric dysfunction are clearly part of the disorder. Approximately 80% of patients with the clinical characteristics of MELAS syndrome have a genetic mutation. This progressive disorder is reported to have a high morbidity and mortality. This case report is intended to estimate clinical effects of oriental meedicinal treatment of MELAS syndrome. Methods : A 44 year old female patient diagnosed as MELSA syndrome was treated with general oriental medicinal therapy including acupuncture, moxibustion, cupping, pharmacupunture and herbal medication in 20XX in Wonkwang Medical Center Gwangju. And the changes in symptoms and signs were evaluated as time dependently. Results : Although there is currently no curable treatment and MEALS syndrome is tend to progress, our treatment showed improvement in general weakness, gait disturbance and pain in the patient. Conclusion : Our case report suggests that various oriental medicinal treatment could be effective for improvement of MELAS syndrome and may represent a new potential therapeutic approach to control the disease. It could be applied to improve general condition, prevent relapse, enhance the quality of life and reduce complaints in the patient.

• Advances in pediatric surgery
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• 제8권1호
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• pp.41-47
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• 2002

장 운동은 장근 및 점막하 신경총으로 구성된 내인성 신경계와 외인성 신경계가 합세하여 균형을 이루면서 장관이 팽창하면 상부는 수축하고 하부는 이완되어 장 내용물이 하방으로 이 동하는 운동이 연속적으로 이어지는 것이다. 신경절 세포가 없으면 내인성 신경계 억제작용 매체인 NO의 결손으로 평활근이 이완되지 않을 뿐더러 외인성 신경계 작용이 장벽에 현저히 증가되어 평소의 2-3배가 되는데 특히 adrenergic 계가 더 작용이 강하여 장벽 긴장도가 증가된 것이 무신경절 장관에서 나타나는 장 운동장애 현상의 병태생리로 설명되고 있다. 이러한 신경총의 부재는 NCC의 이동, 정착 및 성숙에 관여하는 여러 인자들의 복합적인 병적 작용 발현으로 일어나는 발생학적인 현상이며, 이러한 각종 인자들의 결함은 이와 관련된 염색체 혹은 유전자들의 변이 현상에 의한 것으로 밝혀짐에 따라 유전적인 요인들이 깊이 내재되어 있는 것이 하나씩 증명되어가고 있는 단계라고 말 할 수 있다. 지금까지는 HD와 동반되어 나타나는 이러한 현상들을 밝혀 나가고 있는 단계에 불과하며, 이러한 분자생물학적인 지식을 기초로 한다고 하여도 아직은 발병 예방이나 유전적인 치료를 고려할 수 있는 수준에는 미치지 못하는 실정이다.

• Journal of Plant Biotechnology
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• 제37권4호
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• pp.425-441
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• 2010

Forages are the backbone of sustainable agriculture. They includes a wide variety of plant species ranging from grasses, such as tall fescue and bermudagrass, to herbaceous legumes, such as alfalfa and white clover. Abiotic stresses, especially salinity, drought, temperature extremes, high photon irradiance, and levels of inorganic solutes, are the limiting factors in the growth and productivity of major cultivated forage crops. Given the great complexity of forage species and the associated difficulties encountered in traditional breeding methods, the potential from molecular breeding in improving forage crops has been recognized. Plant engineering strategies for abiotic stress tolerance largely rely on the gene expression for enzymes involved in pathways leading to the synthesis of functional and structural metabolites, proteins that confer stress tolerance, or proteins in signaling and regulatory pathways. Genetic engineering allows researchers to control timing, tissue-specificity, and expression level for optimal function of the introduced genes. Thus, the use of either a constitutive or stress-inducible promoter may be useful in certain cases. In this review, we summarize the recent progress made towards the development of transgenic forage plants with improved tolerance to abiotic stresses.

• 드라이브 ㆍ 컨트롤
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• 제13권2호
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• pp.34-41
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• 2016

Unlike the commonly used On/Off solenoid, constant attraction force which is independent of plunger displacement is a considerably important characteristic to proportional solenoid of the EPPR Valve. Attraction force uniformity is mainly affected by the internal shape design parameters. Due to a number of shape design parameters, the optimal parameter values are very complex and time consuming to find by trial and error method. Much research has been conducted or are still in progress to find the optimal parameter values by applying various optimization techniques like Genetic Algorithm, Evolution Strategy, Simulated Annealing, or the Taguchi method. In this paper, the design parameters which have primary effects on the attraction force uniformity and the average attraction force are decided by main effects analysis of Design of Experiments. Optimal parameter values are derived using finite-element analysis and a neural network model.

• Journal of Plant Biotechnology
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• 제47권4호
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• pp.309-315
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• 2020

Advances in biotechnology have led to progress in crop genetic engineering to improve agricultural productivity. The use of genetically modified (GM) crops has increased, as have consumers' and regulators' concerns about the safety of GM crops to human health, and ecological biodiversity. As such, the identification of GM crops is a critical issue for developers and distributors, and their labeling is mandatory. Multiplex polymerase chain reaction (PCR) has been developed and its use validated for the detection and identification of GM crops in quarantine. Herein, we established a simultaneous detection method to identify four GM maize events. Event-specific primers were designed between the junction region of transgene and genome of four GM maize lines, namely 5307, DAS-40278-9, MON87460, and MON87427. To verify the efficiency and accuracy of the multiplex PCR we used specificity analysis, limit of detection evaluation, and mixed certified reference materials identification. The multiplex PCR method was applied to analyze 29 living, modified maize volunteers collected in South Korea in 2018 and 2019. We performed multiplex PCR analysis to identify events and confirmed the result by simplex PCR using each event-specific primer. As a result, rather than detecting each event individually, the simultaneous detection PCR method enabled the rapid analysis of 29 GM maize volunteers. Thus, the novel multiplex PCR method is applicable for living modified organism volunteer identification.

• 대한한의학회지
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• 제42권2호
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• pp.72-81
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• 2021

Objectives: We compared research characteristics of western medicine, Chinese medicine and Korean medicine on causes, mechanisms, types, treatments and prevention of psoriasis. Methods: For western medicine, "Psoriasis" was used as keyword on Pubmed, for Chinese medicine, "銀屑病" and "中医" on CNKI (China National Knowledge Infrastructure" and for Korean medicine, "건선" on OASIS. Keyword searches were done for papers and books published after 2010. For Chinese medicine, there were more in-depth searches done for "從血論 (血熱, 血瘀, 血燥)" and "陽虛症". Results: Western medicine puts an emphasis on the foci, and approaches it from molecular and genetic levels based on molecular biology; while it views psoriasis as a disease with multiple possible causes, it ultimately sees it as an inflammation that is immunity-mediated. Western medicine seeks to suppress cytokine in order to prevent and eliminate inflammation at each stage of treatment While they are effective short-term, psoriasis recurs shortly after. Chinese and Korean medicines categorize psoriasis as an internal comprehensive systemic diseases that encompasses the patient's physical and mental characteristics, and defines it as a disease that has many causes and mechanisms such as "血熱, 血瘀, 血燥" and "陽虛". They use herbal medicine, acupuncture, and lifestyle interventions to improve the overall health of the patient in addition to treating psoriasis. Treatments are effective, but it takes relatively longer to see results, and can recur. Conclusion: In order for more progress to happen on psoriasis treatment, each branch of medicine must exchange knowledge and information more frequently.