• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 춘계학술대회 및 국제심포지움 초록집
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• pp.6-9
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• 2005

Plant biotechnology involving genetic modification has been rather controversial. However, the major issues related to safety are being addressed by continued improvements in technology. Some of the related facts will be highlighted to set the tone for a scientific discussion on the possibilities of using the technology for crop improvement. Our main research interest is to understand the molecular regulation of shoot bud regeneration in plant tissue culture, which is essential for crop improvement by biotechnology. We have isolated and characterized some genes that are associated with adventitious shoot regeneration. These include a MADS-box cDNA (PkMADS1) from paulownia kawakamii, which regulates vegetative shoot development and in vitro shoot regeneration from leaf explants. Another gene we have characterized from petunia codesfor a cytokinin binding protein (PETCBP). Preliminary functional analysis of this gene indicated that this also affects adventitious shoot bud initiation. Also, the antisense suppression of this gene in petunia causedexcessive branching. Results from our work and selected other publications will be used to highlight the possibilities of manipulation of such genes to improve crop species.

• Geomechanics and Engineering
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• 제36권4호
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• pp.329-341
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• 2024

Retaining structures are one of the most important elements in the stabilization of excavations and slopes in various engineering projects. Mechanically stabilized earth (MSE) walls are widely used as retaining structures due to their flexibility, easy and economical construction. These benefits are especially prominent for projects built on soft and weak foundation soils, which have relatively low resistance and high compressibility. For high retaining walls on weak foundations, conventional design methods are not cost-effective. Therefore, two alternative solutions for different foundation weakness are proposed in this research: optimized multi-tiered MSE walls and single tier wall with foundation improvement. The cost optimization considers both the construction components and the land price. The results show that the optimal solution depends on several factors, including the foundation strength and more importantly, the land price. For low land price, the optimized multi-tiered wall is more economical, while for high land price (urban areas), the foundation improvement is preferable. As the foundation strength decreases, the foundation improvement becomes inevitable.

• Industrial Engineering and Management Systems
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• 제11권2호
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• pp.155-164
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• 2012

As the global market becomes more competitive, manufacturing industries face relentless pressure caused by a growing tendency of greater varieties of products, shorter manufacturing cycles and more sophisticated customer requirements. Efficient and effective supplier selection and order allocation decisions are, therefore, important decisions for a manufacturer to ensure stable material flows in a highly competitive supply chain, in particular, when customers are willing to accept products with less desirable product attributes (e.g., color, delivery date) for economic reasons. This paper attempts to solve optimally the challenging problem of supplier selection and order allocation, taking into consideration the customer flexibility for a manufacturer producing multi-products to satisfy the customers' demands in a multi period planning horizon. A new mixed integer programming model is developed to describe the behavior of the supply chain. The objective is to maximize the manufacturer's total profit subject to various operating constraints of the supply chain. Due to the complexity and non-deterministic polynomial-time (NP)-hard nature of the problem, an improved genetic approach is proposed to solve the problem optimally. This approach differs from a canonical genetic algorithm in three aspects: a new selection method to reduce the chance of premature convergence and two problem-specific repair heuristics to guarantee feasibility of the solutions. The results of applying the proposed approach to solve a set of randomly generated test problems clearly demonstrate its excellent performance. When compared with applying the canonical genetic algorithm to locate optimal solutions, the average improvement in the solution quality amounts to as high as ten percent.

• Asian-Australasian Journal of Animal Sciences
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• 제11권5호
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• pp.463-469
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• 1998

Factor analysis was applied as a multivariate statistical technique to official genetic evaluations of type classification traits for 1,265,785 Holstein cows and 10,321 sires computed from data collected between August 1982 and June 1994 in Canada. Type traits included eighteen linear descriptive traits and eight major score card traits. Principal components of the factor analysis showed that only five factors explain the information of the genetic value of linear descriptive traits for both cows and sires. Factor 1 included traits related to mammary system, like texture, median suspensory, fore attachment, fore teat placement and rear attachment height and width. Factor 2 described stature, size, chest width and pin width. These two factors had a similar pattern for both cows and sires. In constrast, Factor 3 for cows involved only bone-quality, while in addition for sires, Factor 3 included foot angle, rear legs desirability and legs set. Factor 4 for cows related to foot angle, set of rear leg and leg desirability, while Factor 4 related to loin strenth and pin setting for sires. Finally, Factor 5 included loin strength and pin setting for cows and described only pin setting for sires. Two factors only were required to describe score card traits of cows and sires. Factor 1 related to final score, feet and legs, udder traits, mammary system and dairy character, while frame/capacity and rump were described by Factor 2. Communality estimates which determine the proportion of variance of a type trait that is shared with other type traits via the common factor variant were high, the highest ${\geq}$ 80% for final score, stature, size and chest width. Pin width and pin desirability had the lowest communality, 56% and 37%. Results indicated shifts in emphasis over the twelve-year period away from udder traits and dairy character, and towards size, scale and width traits. A new system that computes fmal score from type components has been initiated.

• Asian-Australasian Journal of Animal Sciences
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• 제30권8호
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• pp.1074-1080
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• 2017

Objective: The growth, carcass and retail cut yield records on 1,428 Hanwoo steers obtained through progeny testing were analyzed in this study, and their heritability and genetic relationships among the traits were estimated using animal models. Methods: Two different models were compared in this study. Each model was fitted for different fixed class effects, date of slaughter for carcass traits and batch of progeny test live measurement traits, and a choice of covariates (carcass weight in Model 1 or backfat thickness in Model 2) for carcass traits. Results: The differences in body composition among individuals were deemed being unaffected by their age at slaughter, except for carcass weight and backfat thickness. Heritability estimates of body size measurements were 0.21 to 0.36. Heritability estimates of retail cut percentage were high (0.56 from Model 1 and 0.47 from Model 2). And the heritability estimates for loin muscle percentage were 0.36 from Model 1 and 0.42 from Model 2, which were high enough to consider direct selection on carcass cutability traits as effective. The genetic correlations between body size measurements and retail cut ratio (RCR) were close to zero. But, some negative genetic correlations were found with chest girths measured at yearling (Model 1) or at 24 months of age or with chest widths. Loin muscle ratio (LMR) was genetically negatively correlated with body weights or body size measurements, in general in Model 1. These relationships were low close to zero but positive in Model 2. Phenotypic correlation between cutability traits (RCR, LMR) and live body size measurements were moderate and negative in Model 1 while those in Model 2 were all close to zero. Conclusion: Therefore, the body weights or linear body measurements at an earlier age may not be the most desirable selection traits for exploitation of correlated responses to improve loin muscle or lean meat yield.

• Journal of Animal Science and Technology
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• 제44권2호
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• pp.201-206
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• 2002

본 연구는 한우개량에 필요한 기초자료를 얻기 위하여 1979년 부터 1995년 사이에 전국 한우개량단지에서 조사한 체중과 10개 부위의 체척치에 대한 자료를 가지고 유전모수 추정에 이용하였으며 얻어진 결과를 요약하면 다음과 같다. 1) 유전력의 추정치는 생시, 이유시, 12개월령 및 27개월령 체중에서 각각 0.208${\pm}$0.038, 0.457${\pm}$0.073, 0.476${\pm}$0.082 및 0.227${\pm}$0.040으로 추정되었으며 12개월령 및 27개월령의 체고, 체장 및 흉위에서 각각 0.326, 0.486, 0.462 및 0.242, 0.110, 0.170으로 추정되었다. 2) 12개월령 체중과 체척치들간의 유전상관과 표현형상관은 모두 정의 값으로 추정되었으며 그 범위는 0.223부터 0.999까지 이었다. 27개월령 체중과 체척치들간의 유전상관과 표현형상관도 모두 정의 값으로 추정되었으며 그 범위는 0.290 부터 0.083까지 이었다.

• 한국수정란이식학회지
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• 제28권2호
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• pp.79-85
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• 2013

The recent development in genetic assisted selection (combining traditional- and genome assisted selection method) and reproduction technologies will allow multiplying elite cow in Hanwoo small farm. This review describes the new context and corresponding needs for genome assisted selection schemes and how reproductive technologies can be incorporated to get more genetic gain for cow genetic improvement in Hanwoo. New improved massive phenotypes and pedigree information are being generated from commercial farm sector and these are allowing to do genetic evaluation using BLUP to get elite cows in Korea. Moreover cattle genome information can now be incorporated into breeding program. In this context, this review will discuss about combining the reproductive techniques (Multiple Ovulation Embryo Transfer; MOET) and genome assisted selection method to get more genetic gain in Hanwoo breeding program. Finally, how these technologies can be used for multiplication of elite cow in small farm was discussed.

• Asian-Australasian Journal of Animal Sciences
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• 제27권9호
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• pp.1368-1372
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• 2014

Several studies arduously reported that gayal (Bos frontalis) is an independent bovine species. The population size is shrinking across its distribution. In Bangladesh, it is the only wild relative of domestic cattle and also a less cared animal. Their body size is much bigger than Bangladeshi native cattle and has prominent beef type characters along with the ability to adjust in any adverse environmental conditions. Human interactions and manipulation of biodiversity is affecting the habitats of gayals in recent decades. Besides, the only artificial reproduction center for gayals, Bangladesh Livestock Research Institute (BLRI), has few animals and could not carry out its long term conservation scheme due to a lack of an objective based scientific mission as well as financial support. This indicates that the current population is much more susceptible to stochastic events which might be natural catastrophes, environmental changes or mutations. Further reduction of the population size will sharply reduce genetic diversity. In our recent investigation with 80K indicine single nucleotide polymorphism chip, the $F_{IS}$ (within-population inbreeding) value was reported as $0.061{\pm}0.229$ and the observed ($0.153{\pm}0.139$) and expected ($0.148{\pm}0.143$) heterozygosities indicated a highly inbred and less diverse gayal population in Bangladesh. Prompt action is needed to tape the genetic information of this semi-domesticated bovine species with considerable sample size and try to investigate its potentials together with native zebu cattle for understanding the large phenotypic variations, improvement and conservation of this valuable creature.

• Asian-Australasian Journal of Animal Sciences
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• 제27권9호
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• pp.1263-1269
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• 2014

Comprehensive information on genetic diversity and introgression is desirable for the design of rational breed improvement and conservation programs. Despite the concerns regarding the genetic introgression of Western pig breeds into the gene pool of the Korean native pig (KNP), the level of this admixture has not yet been quantified. In the present study, we genotyped 93 animals, representing four Western pig breeds and KNP, using the porcine SNP 60K BeadChip to assess their genetic diversity and to estimate the level of admixture among the breeds. Expected heterozygosity was the lowest in Berkshire (0.31) and highest in Landrace (0.42). Population differentiation ($F_{ST}$) estimates were significantly different (p<0.000), accounting for 27% of the variability among the breeds. The evidence of inbreeding observed in KNP (0.029) and Yorkshire (0.031) may result in deficient heterozygosity. Principal components one (PC1) and two (PC2) explained approximately 35.06% and 25.20% of the variation, respectively, and placed KNP somewhat proximal to the Western pig breeds (Berkshire and Landrace). When K = 2, KNP shared a substantial proportion of ancestry with Western breeds. Similarly, when K = 3, over 86% of the KNP individuals were in the same cluster with Berkshire and Landrace. The linkage disquilbrium (LD) values at $r^2_{0.3}$, the physical distance at which LD decays below a threshold of 0.3, ranged from 72.40 kb in Landrace to 85.86 kb in Yorkshire. Based on our structure analysis, a substantial level of admixture between Western and Korean native pig breeds was observed.

• Asian-Australasian Journal of Animal Sciences
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• 제18권3호
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• pp.301-307
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• 2005

Genetic variants of Hanwoo mtDNA in the region of cytochrome oxidase subunit I, II and III complex were detected using restriction enzymes. PCR primers were designed based on the bovine mtDNA sequence, and 6 primer sets (Mt4, Mt5, Mt6, Mt7, Mt8 and Mt9) were used. A total of 20 restriction enzymes were used, and 6 restriction enzymes, which were Hinf I, Pvu II, Rsa I, Eco RI, Bgl II, and Msp I, showed genetic polymorphisms. Significant associations between genetic variants and weight traits were observed at WT15 (p<0.05) and WT18 (p<0.01) with Pvu II for Mt9, Bgl II for Mt6 and Rsa I for Mt8 segments in the region of cytochrome oxidase subunit complex. Significant associations were also observed at Mt9-Pvu II and Mt6-Bgl II segments for WT9 (p=0.01), WT12 (p=0.02), respectively. These results suggest that genetic variants of mtDNA in the region of cytochrome oxidase subunit complex may be candidate segments for improvement of animal growth as weight traits.