• 한국환경성돌연변이발암원학회지
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• 제22권2호
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• pp.90-96
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• 2002

Benzene is a widespread human carcinogen, inducing leukemia and hematotoxicity. Exposure to benzene metabolites has been shown to cause genetic damage, including aneusomy and chromosome aberrations. Fluorescence in situ hybridization(FISH) procedure was used to determine if the benzene metabolite, 1, 2, 4-benzenetriol(BT), hydroquinone(HQ) and trans, trans-muconic acid(t,t-MA) induced specific chromosomal change in HL-60 cells. Treatment with BT, HQ and t,t-MA resulted in the induction of monosomy 8 and 21 in HL-60 cells in a dose-dependent manner. All of these metabolites also induced trisomy 8 and 21, but no correlation between frequencies of trisomy and concentration was found. Translocations between chromosome 8 and another unidentified chromosome ［t(8:\ulcorner)］, and between chromosome 21 and another unidentified chromosome ［t(8:21)］ were found. However, translocation between chromosome 8 and 21 ［t(8:21)］ was not found. Results indicate that the benzene metabolites, BT, HQ and t,t-MA, induce chromosome specific numerical and structural aberrations, and the fluorescence in situ hybridization (FISH) approach may be a useful and powerful technique for detection of aneuploidy.

• 대한의생명과학회지
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• 제12권3호
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• pp.209-215
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• 2006

The mutability and frequency of genetic reassortment characteristic of rotavirus and resultant antigenic changes make the rotavirus formidable challenges for control efforts such as the vaccine development. An alternative approach to overcome these difficulties in development of the rotavirus vaccine is to develop effective inhibitors of the virus infection. As an effort to achieve this, effects of glycyrrhetinic acid (GA), which is an active component of glycyrrhizin, on MA-14 cell infection were examined by employing the human rotavirus isolated from Korea, K-21. The data obtained showed that MA-104 cell infection of the K-21 rotavirus was greatly influenced by the presence of both $18{\alpha}-Ga\;and\;18{\beta}-GA$. Both types of GA have inhibited more than 60% of the rotaviral infection at the concentration of 7.68mM. This inhibition effect became much more evident at the higher concentrations of GA. However, the type of GA did not make much differences on the inhibition effect of the drug. Although GA has to be used in high concentrations to exhibit anti-viral activity and to be virostatic, a long history of safe and high dose usage of licoriece in clinical settings in the Far East makes the GA as an attractive inhibitor of the rotaviral infection.

• 생태와환경
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• 제36권3호통권104호
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• pp.221-234
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• 2003

종다양성 규명을 위한 계통분류학적 연구는 원시형질의 상동성에 근거한 기존의 형태형질과 효율적인 새로운 분자계통분류학적 연구방법이 병행된다면 좀 더 정확한 분류 및 계통을 추적하는데 객관적인 결론을 제시 할수 있을 것이다. 현재 활발히 연구되고 있는 미토콘드리아 DNA를 이용한 분자계통분류를 재검토할 뿐아니라 계통진화학상 조상과 자손과의 관계를 유추하는데 새로운 가설을 세울 수 있는 토대를 제공하는 기초자료로서 이용될 수 있다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제9권12호
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• pp.4776-4798
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• 2015

The location selection of virtual machines in distributed cloud is difficult because of the physical resource distribution, allocation of multi-dimensional resources, and resource unit cost. In this study, we propose a multi-object virtual machine location selection algorithm (MOVMLSA) based on group information, doubly linked list structure and genetic algorithm. On the basis of the collaboration of multi-dimensional resources, a fitness function is designed using fuzzy logic control parameters, which can be used to optimize search space solutions. In the location selection process, an orderly information code based on group and resource information can be generated by adopting the memory mechanism of biological immune systems. This approach, along with the dominant elite strategy, enables the updating of the population. The tournament selection method is used to optimize the operator mechanisms of the single-point crossover and X-point mutation during the population selection. Such a method can be used to obtain an optimal solution for the rapid location selection of virtual machines. Experimental results show that the proposed algorithm is effective in reducing the number of used physical machines and in improving the resource utilization of physical machines. The algorithm improves the utilization degree of multi-dimensional resource synergy and reduces the comprehensive unit cost of resources.

• 한국정밀공학회지
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• 제30권11호
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• pp.1153-1160
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• 2013

SMISMO valve configuration is now starting to draw attention of the researchers of the construction equipment industry to increase the fuel efficiency of their equipment like excavators and wheel-loaders. An open-loop control strategy for grading operation of hydraulic excavator systems using SMISMO valve configuration is investigated in this paper. Tabor's algorithm for 1 d.o.f. SMISMO system under the assumption of quasi-static operation is revealed as not adequate for multi d.o.f. system with large moment of inertia even though the motion of the system is slow. New parameters are proposed in this paper. It modifies Tabor's open-loop control strategy for the grading operation of hydraulic excavators using SMISMO valve configuration. A simulation-based parameter tuning method is also proposed. It uses GA (Genetic Algorithm) to find the best parameter values. Simulation study for a practical hydraulic excavator shows the validity of the proposed open-loop control strategy.

• Plant Biotechnology Reports
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• 제5권1호
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• pp.1-7
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• 2011

Secondary walls have recently drawn research interest as a primary source of sugars for liquid biofuel production. Secondary walls are composed of a complex mixture of the structural polymers cellulose, hemicellulose, and lignin. A matrix of hemicellulose and lignin surrounds the cellulose component of the plant's cell wall in order to protect the cell from enzymatic attacks. Such resistance, along with the variability seen in the proportions of the major components of the mixture, presents process design and operating challenges to the bioconversion of lignocellulosic biomass to fuel. Expanding bioenergy production to the commercial scale will require a significant improvement in the growth of feedstock as well as in its quality. Plant biotechnology offers an efficient means to create "targeted" changes in the chemical and physical properties of the resulting biomass through pathway-specific manipulation of metabolisms. The successful use of the genetic engineering approach largely depends on the development of two enabling tools: (1) the discovery of regulatory genes involved in key pathways that determine the quantity and quality of the biomass, and (2) utility promoters that can drive the expression of the introduced genes in a highly controlled manner spatially and/or temporally. In this review, we summarize the current understanding of the transcriptional regulatory network that controls secondary wall biosynthesis and discuss experimental approaches to developing-xylem-specific utility promoters.

• Molecular & Cellular Toxicology
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• 제3권3호
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• pp.151-158
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• 2007

The failure of injured axons to regenerate in the mature central nervous system (CNS) has devastating consequences for victims of spinal cord injury (SCI). Traditional strategies to treat spinal cord injured people by using drug therapy and assisting devices that can not help them to recover fully various vital functions of the spinal cord. Many researches have been focused on accomplishing re-growth and reconnection of the severed axons in the injured region. Using cell transplantation to promote neural survival or growth has had modest success in allowing injured neurons to re-grow through the area of the lesion. Strategies for successful regeneration will require tissue engineering approach. In order to persuade sufficient axons to regenerate across the lesion to bring back substantial neurological function, it is necessary to construct an efficient biocompatible bridge (cell-free or implanted with different cell lines as hybrid implant) through the injured area over which axons can grow. Therefore, in this paper, spinal cord and its injury, different strategies to help regeneration of an injured spinal cord are reviewed. In addition, different aspects of designing a biocompatible bridge and its applications and challenges surrounding these issues are also addressed. This knowledge is very important for the development and optimalization of therapies to repair the injured spinal cord.

• 대한수의학회지
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• 제35권2호
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• pp.307-315
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• 1995

The characterization of the MHC of domestic animals may constitute a first step towards increasing the efficiency of food production through improved disease resistance. In order to study the role of the MHC in regulating immune response it is first necessary to identify the different MHC alleles. In this research we try to investigate the possible associations between BoLA of Korean native cattles and infectious cattle disease. For this purpose we used one approach, serology. The results were summarized as follows : 1. Korean native cattle's lymphocyte reacted with alloantisera which recognized seven official BoLA allele. Korean native cattle's lymphocytes were reacted same as European breeds(especially with 673/3(W20)). 2. Korean native cattle's lymphocytes reacted with alloantisera 773/2, 673/3, 638/3, 773/3, 602/2, 639/2 and 639/3 at high reaction frequency. But alloantisera 642/1 was not expressed on Korean native cattle. If this allele, recognized by alloantisera(642/1), officially certificate In BoLA workshop it will be characterization factor of Korean native cattle. 3. According to cellular similarity index, we can presume on genetic relativity which has no family relationship.

• 한국강구조학회 논문집
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• 제22권2호
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• pp.197-208
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• 2010

언더텐션이란 상부에서의 하중을 하부 케이블의 인장력을 이용하여 그 하중을 양 단부로 전달하는 시스템을 말하며 처짐과 부재 크기를 제어하기 위해 장스팬 구조물에 주로 적용된다. 하지만 수많은 단면 중에서 여러 조건을 만족하며 최소의 물량, 즉 최대의 경제성에 부합하는 최적의 단면을 찾는 것은 매우 어려운 일이다. 이에 본 연구에서는 마이크로 유전알고리즘을 이용하여 언더텐션 형태의 트러스 구조물에 단면 최적화 연구를 수행하였으며 처짐과 허용응력 그리고 좌굴이 제약조건으로 고려되었다. 최적화 검증 예제로 자주 사용되는 10-bar 트러스 예제의 이전 연구 결과와의 비교를 통하여 개발된 프로그램을 검증하였다. 이를 바탕으로 프리텐션 크기를 변수로 설정하여 언더텐션 구조물의 최적 설계를 수행하였다. 본 연구에서 제시된 최적설계 그래프를 통하여 설계자가 일정 조건을 만족하는 최적의 단면을 쉽게 선택할 수 있을 것으로 사료된다.

• Journal of Electrical Engineering and Technology
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• 제8권3호
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• pp.402-410
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• 2013

In this paper, a novel and robust Power System Stabilizer (PSS) is proposed as an effective approach to improve stability in electric power systems. The dynamic performance of proposed PSS has been thoroughly compared with Conventional PSS (CPSS). Both the Real Coded Genetic Algorithm (RCGA) and Particle Swarm Optimization (PSO) techniques are applied to optimum tune the parameter of both the proposed PSS and CPSS in order to damp-out power system oscillations. Due to the high sufficiency of both the RCGA and PSO techniques to solve the very non-linear objective, they have been employed for solution of the optimization problem. In order to verify the dynamic performance of these devices, different conditions of disturbance are taken into account in Single Machine Infinite Bus (SMIB) power system. Moreover, to ensure the robustness of proposed PSS in damping the power system multi-mode oscillations, a Multi Machine (MM) power system under various disturbances are considered as a test system. The results of nonlinear simulation strongly suggest that the proposed PSS significantly enhances the power system dynamic stability in both of the SMIB and MM power system as compared to CPSS.