• Korean Journal of Ecology and Environment
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• v.36 no.3 s.104
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• pp.221-234
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• 2003

Phylogenetic studies would clarify the diversity of fishes if the morphological analysis based on plesimorphy characters combined with new genetic analysis on molecular level, inferring more accurate and objective phylogeny and the taxonomy. Current molecular phylogenetic approach using mitochondrial genome provides the framework for a new hypothesis not only inferring the relationships between ancestor descendants but raveling the intra-, interspecies variation.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.9 no.12
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• pp.4776-4798
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• 2015

The location selection of virtual machines in distributed cloud is difficult because of the physical resource distribution, allocation of multi-dimensional resources, and resource unit cost. In this study, we propose a multi-object virtual machine location selection algorithm (MOVMLSA) based on group information, doubly linked list structure and genetic algorithm. On the basis of the collaboration of multi-dimensional resources, a fitness function is designed using fuzzy logic control parameters, which can be used to optimize search space solutions. In the location selection process, an orderly information code based on group and resource information can be generated by adopting the memory mechanism of biological immune systems. This approach, along with the dominant elite strategy, enables the updating of the population. The tournament selection method is used to optimize the operator mechanisms of the single-point crossover and X-point mutation during the population selection. Such a method can be used to obtain an optimal solution for the rapid location selection of virtual machines. Experimental results show that the proposed algorithm is effective in reducing the number of used physical machines and in improving the resource utilization of physical machines. The algorithm improves the utilization degree of multi-dimensional resource synergy and reduces the comprehensive unit cost of resources.

• Journal of the Korean Society for Precision Engineering
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• v.30 no.11
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• pp.1153-1160
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• 2013

SMISMO valve configuration is now starting to draw attention of the researchers of the construction equipment industry to increase the fuel efficiency of their equipment like excavators and wheel-loaders. An open-loop control strategy for grading operation of hydraulic excavator systems using SMISMO valve configuration is investigated in this paper. Tabor's algorithm for 1 d.o.f. SMISMO system under the assumption of quasi-static operation is revealed as not adequate for multi d.o.f. system with large moment of inertia even though the motion of the system is slow. New parameters are proposed in this paper. It modifies Tabor's open-loop control strategy for the grading operation of hydraulic excavators using SMISMO valve configuration. A simulation-based parameter tuning method is also proposed. It uses GA (Genetic Algorithm) to find the best parameter values. Simulation study for a practical hydraulic excavator shows the validity of the proposed open-loop control strategy.

• Plant Biotechnology Reports
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• v.5 no.1
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• pp.1-7
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• 2011

Secondary walls have recently drawn research interest as a primary source of sugars for liquid biofuel production. Secondary walls are composed of a complex mixture of the structural polymers cellulose, hemicellulose, and lignin. A matrix of hemicellulose and lignin surrounds the cellulose component of the plant's cell wall in order to protect the cell from enzymatic attacks. Such resistance, along with the variability seen in the proportions of the major components of the mixture, presents process design and operating challenges to the bioconversion of lignocellulosic biomass to fuel. Expanding bioenergy production to the commercial scale will require a significant improvement in the growth of feedstock as well as in its quality. Plant biotechnology offers an efficient means to create "targeted" changes in the chemical and physical properties of the resulting biomass through pathway-specific manipulation of metabolisms. The successful use of the genetic engineering approach largely depends on the development of two enabling tools: (1) the discovery of regulatory genes involved in key pathways that determine the quantity and quality of the biomass, and (2) utility promoters that can drive the expression of the introduced genes in a highly controlled manner spatially and/or temporally. In this review, we summarize the current understanding of the transcriptional regulatory network that controls secondary wall biosynthesis and discuss experimental approaches to developing-xylem-specific utility promoters.

• Molecular & Cellular Toxicology
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• v.3 no.3
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• pp.151-158
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• 2007

The failure of injured axons to regenerate in the mature central nervous system (CNS) has devastating consequences for victims of spinal cord injury (SCI). Traditional strategies to treat spinal cord injured people by using drug therapy and assisting devices that can not help them to recover fully various vital functions of the spinal cord. Many researches have been focused on accomplishing re-growth and reconnection of the severed axons in the injured region. Using cell transplantation to promote neural survival or growth has had modest success in allowing injured neurons to re-grow through the area of the lesion. Strategies for successful regeneration will require tissue engineering approach. In order to persuade sufficient axons to regenerate across the lesion to bring back substantial neurological function, it is necessary to construct an efficient biocompatible bridge (cell-free or implanted with different cell lines as hybrid implant) through the injured area over which axons can grow. Therefore, in this paper, spinal cord and its injury, different strategies to help regeneration of an injured spinal cord are reviewed. In addition, different aspects of designing a biocompatible bridge and its applications and challenges surrounding these issues are also addressed. This knowledge is very important for the development and optimalization of therapies to repair the injured spinal cord.

• Korean Journal of Veterinary Research
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• v.35 no.2
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• pp.307-315
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• 1995

The characterization of the MHC of domestic animals may constitute a first step towards increasing the efficiency of food production through improved disease resistance. In order to study the role of the MHC in regulating immune response it is first necessary to identify the different MHC alleles. In this research we try to investigate the possible associations between BoLA of Korean native cattles and infectious cattle disease. For this purpose we used one approach, serology. The results were summarized as follows : 1. Korean native cattle's lymphocyte reacted with alloantisera which recognized seven official BoLA allele. Korean native cattle's lymphocytes were reacted same as European breeds(especially with 673/3(W20)). 2. Korean native cattle's lymphocytes reacted with alloantisera 773/2, 673/3, 638/3, 773/3, 602/2, 639/2 and 639/3 at high reaction frequency. But alloantisera 642/1 was not expressed on Korean native cattle. If this allele, recognized by alloantisera(642/1), officially certificate In BoLA workshop it will be characterization factor of Korean native cattle. 3. According to cellular similarity index, we can presume on genetic relativity which has no family relationship.

• Journal of Korean Society of Steel Construction
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• v.22 no.2
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• pp.197-208
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• 2010

An under-tension system is frequently employed for large-span structures to reduce the deflection and member size. In this study, a microgenetic algorithm was used to find the optimum cross-section of truss structures with an undertension cable under transverse loading. Maximum deflection, allowable stress, and buckling were considered constraints. The proposed approach was verified using a 10-bar truss sample that shows good agreement with the previous results. In the numerical results, minimum-weight design of the under-tension structure was performed for various magnitudes of pretension.

• Journal of Electrical Engineering and Technology
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• v.8 no.3
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• pp.402-410
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• 2013

In this paper, a novel and robust Power System Stabilizer (PSS) is proposed as an effective approach to improve stability in electric power systems. The dynamic performance of proposed PSS has been thoroughly compared with Conventional PSS (CPSS). Both the Real Coded Genetic Algorithm (RCGA) and Particle Swarm Optimization (PSO) techniques are applied to optimum tune the parameter of both the proposed PSS and CPSS in order to damp-out power system oscillations. Due to the high sufficiency of both the RCGA and PSO techniques to solve the very non-linear objective, they have been employed for solution of the optimization problem. In order to verify the dynamic performance of these devices, different conditions of disturbance are taken into account in Single Machine Infinite Bus (SMIB) power system. Moreover, to ensure the robustness of proposed PSS in damping the power system multi-mode oscillations, a Multi Machine (MM) power system under various disturbances are considered as a test system. The results of nonlinear simulation strongly suggest that the proposed PSS significantly enhances the power system dynamic stability in both of the SMIB and MM power system as compared to CPSS.

• Genomics & Informatics
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• v.6 no.2
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• pp.84-86
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• 2008

The Tetrahymena group I intron has been shown to employ a trans-splicing reaction and has been modified to specifically target and replace human telomerase reverse transcriptase (hTERT) RNA with a suicide gene transcript, resulting in the induction of selective cytotoxicity in cancer cells that express the target RNA, in animal models as well as in cell cultures. In this study, we evaluated the target RNA specificity of trans-splicing phenomena by the group I intron in mice that were intraperitoneally inoculated with hTERT-expressing human cancer cells to validate the anti-cancer therapeutic applicability of the group I intron. To this end, an adenoviral vector that encoded for the hTERT-targeting group I intron was constructed and systemically injected into the animal. 5'-end RACE-PCR and sequencing analyses of the trans-spliced cDNA clones revealed that all of the analyzed products in the tumor tissue of the virus-infected mice resulted from reactions that were generated only with the targeted hTERT RNA. This study implies the in vivo target specificity of the trans-splicing group I intron and hence suggests that RNA replacement via a trans-splicing reaction by the group I intron is a potent anti-cancer genetic approach.

• Journal of mucopolysaccharidosis and rare diseases
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• v.2 no.1
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• pp.13-16
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• 2016

Mucolipidosis (ML) II/III are autosomal recessive diseases caused by deficiency of post-translational modification of lysosomal enzymes. The mannose-6-phosphate (M6P) residue in lysosomal enzymes synthesized by N-acetylglucosamine 1-phosphotransferase (GlcNAc-phosphotransferase) serves as recognition marker for trafficking in lysosomes. GlcNAc-phosphotransferase is encoded by GNPTAB and GNPTG. Mutations in GNPTAB cause severe ML II alpha/beta and the attenuated ML III alpha/beta. Whereas mutations in GNPTG cause the ML III gamma, the attenuated type of ML III variant. For the diagnostic approaches, increased urinary oligosaccharides excretion could be a screening test in clinically suspicious patients. To confirm the diagnosis, instead of measuring the activity of GlcNAc phosphotransferase, measuring the enzymatic activities of different lysosomal hydrolases are useful for diagnosis. The activities of several lysosomal hydrolases are decreased in fibroblasts but increased in serum of the patients. In addition, the sequence analysis of causative gene is warranted. Therefore, the confirmatory diagnosis requires a combination of clinical evaluation, biochemical and molecular genetic testing. ML II/III show complex disease manifestations with lysosomal storage as the prime cellular defect that initiates consequential organic dysfunctions. As there are no specific therapy for ML to date, understanding the molecular pathogenesis can contribute to develop new therapeutic approaches ultimately.