• Korean Journal of Microbiology
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• v.38 no.4
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• pp.239-239
• /
• 2002

• Proceedings of the Korean Society of Life Science Conference
• /
• 2003.05a
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• pp.140-140
• /
• 2003

• Proceedings of the Zoological Society Korea Conference
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• 2000.10a
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• pp.59.1-59
• /
• 2000

No Abstract, See Full Text

• Proceedings of the Botanical Society of Korea Conference
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• 2002.04a
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• pp.100-100
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• 2002

• Proceedings of the Zoological Society Korea Conference
• /
• 1997.10b
• /
• pp.75-75
• /
• 1997

No Abstract, See Full Text

• Proceedings of the PSK Conference
• /
• 2000.10a
• /
• pp.244.3-245
• /
• 2000

• Proceedings of the PSK Conference
• /
• 2005.11a
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• pp.218-218
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• 2005

• Proceedings of the PSK Conference
• /
• 2000.10a
• /
• pp.245.1-245.1
• /
• 2000

• Proceedings of the PSK Conference
• /
• 2001.10a
• /
• pp.183.1-183.1
• /
• 2001

• Journal of Microbiology and Biotechnology
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• v.18 no.2
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• pp.334-337
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• 2008

A tightly regulated gene expression system composed of a single-copy target gene under the control of a lac promoter derivative and lacI gene in a multicopy plasmid is proposed, and its ability to control the flux of a metabolic pathway is demonstrated. A model system to control the flux of acetyl-CoA to acetyl phosphate was constructed by integrating pta, a gene encoding phosphotransacetylase, under a tac promoter into the chromosome of E. coli with a pta-negative background and transforming a multicopy plasmid containing the $lacI^Q$ gene into the strain. The production rate of acetate was shown to be tightly controlled when varying the concentration of the inducer (IPTG) in he model system.