• Journal of Microbiology and Biotechnology
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• v.34 no.6
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• pp.1249-1259
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• 2024

It remains to be determined whether there is a geographical distribution pattern and phylogenetic signals for the Mycena strains with seed germination of the orchid plant Gastrodia elata. This study analyzed the community composition and phylogenetics of 72 Mycena strains associated with G. elata varieties (G. elata. f. glauca and G. elata. f. viridis) using multiple gene fragments (ITS+nLSU+SSU). We found that (1) these diverse Mycena phylogenetically belong to the Basidiospore amyloid group. (2) There is a phylogenetic signal of Mycena for germination of G. elata. Those strains phylogenetically close to M. abramsii, M. polygramma, and an unclassified Mycena had significantly higher germination rates than those to M. citrinomarginata. (3) The Mycena distribution depends on geographic site and G. elata variety. Both unclassified Mycena group 1 and the M. abramsii group were dominant for the two varieties of G. elata; in contrast, the M. citrinomarginata group was dominant in G. elata f. glauca but absent in G. elata f. viridis. Our results indicate that the community composition of numerous Mycena resources in the Zhaotong area varies by geographical location and G. elata variety. Importantly, our results also indicate that Mycena's phylogenetic status is correlated with its germination rate.

• Journal of Physiology & Pathology in Korean Medicine
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• v.26 no.5
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• pp.738-744
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• 2012

Gastrodia elata Blume is used for a variety of purposes including treatment of inflammation in the Korean medicine. The present study investigated whether the G. elata extracts have the anti-inflammatory effect on lipopolysaccharide(LPS)-stimulated BV-2 microglia cells. G. elata extracts showed an anti-inflammatory effects in the morphological and nitric oxide(NO) analysis, especially in hexane extract. So we investigated the hexane extract from G. elata in the following experiments. Hexane extract significantly inhibited the secretion of NO with protein level of inducible nitric oxide synthase in LPS-stimulated BV2 microglia cells. Hexane extract also inhibited LPS-stimulated inflammatory responses involving the degradation of cytosolic inhibitory(I)-${\kappa}B{\alpha}$ and the translocation of nuclear factor(NF)-${\kappa}Bp65$ to nucleus in LPS-stimulated BV-2 microglia cells by morphological analysis. Western blot analyse confirmed that I-${\kappa}B{\alpha}$ and NF-${\kappa}Bp65$ showed a similar pattern as morphological analysis. Our results suggest that G. elata extracts, especially hexane extract, may act as a therapeutic agent for inflammatory disease in the central nervous system through a selective regulation of NO production and NF-${\kappa}B$ activation.

• Korean Journal of Environment and Ecology
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• v.21 no.5
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• pp.415-431
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• 2007

The native vascular plants, growing at the nature sabbatical application area, were classified & recorded as a total of 407 taxa, 83 families, 235 genera, 338 species, 3 subspecies, 54 varieties and 12 forms. The endangered wild plants were recorded as 2 taxa (Paeonia obovata and Lilium cernuum) and the Korean endemic plants were observed to exist in the formation of 21 taxa, 13 families, 19 genera, 15 species, 4 varieties and 2 forms including Cephalotaxus koreana, Pseudostellaria coreana, Aconitum pseudo-laeve, Clematis trichotoma, Geranium koreanum for. hirsutum, Thalictrum uchiyamai. The floristic special plants consist of 55 taxa, 35 families, 50 genera, 51 species, 4 varieties(Crypsinus hastatus, Cimicifuga heracleifolia, Paeonia obovata, Lilium cernuum and Gastrodia elata etc.). The naturalized plants comprise 10 taxa, 4 families, 10 genera, 9 species and 1 variety. The ratio of Urbanization and Naturalized Index was 3.51% and 2.46%, respectively. Life form spectra were displayed as H-R5-D4-e type.