• 대한한방내과학회지
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• 제15권2호
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• pp.197-208
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• 1994

In order to investigate the efficacy of Siljangsan by using the experimental animals, the action of gastric juice secretion, antiulcer. transport rates in the intestine and anti-cathartic action were studied. The following results were obtained. 1. Preventive effects on ulcer induced by pylorus-ligated and Ethanol-HCI were recognized 2. Suppression effects on gastric juice secretinon, free of acidity and total acidity were recognized, but pepsin output was not recognized. 3. For the transport ability of intestine of mice, the ability of small intestine was activated and large intestine was suppressed in the group administrated 4. Anti-cathatic action of mice induced by castor oil was recognized. According to above results, effect based on oriental medical reference was consistent with the actual experimental effect.

• Biomolecules & Therapeutics
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• 제7권3호
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• pp.285-291
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• 1999

DA-7101 is a new combined formulation of cefatrizine:clavulanic acid (2:1) under development as oral abtibiotics. The general pharmacological properties of DA-7101 on central nervous, cardiovascular, gas-trointestinal and other organ systems were studied by oral administration, in vivo and in vitro. DA-7101 had no marked effects all tests studied such as general behavior, hexobarbital-induced sleeping, spontaneous activity, anticonvulsion, body temperature, acetic acid-induced writhing, rotarod performance, heart rate and blood pressure in cats, isolated ileum movement, intestinal transition, gastric juice secretion and urine volume and electrolytes in rats. But exceptionally at the highest dose of 900 mg/kg, DA-7101 increased hexobarbital-induced sleeping time, caused a slight hypotension and decreased the secretion of gastric juice. These results suggest that at the estimated clinical dose DA-7101 would not bring about any serious acute adverse effects clinically.

• Journal of Gastric Cancer
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• 제18권2호
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• pp.134-141
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• 2018

Purpose: Postoperative pancreatic fistula is a serious and fatal complication of gastrectomy for gastric cancer. Blunt trauma to the parenchyma of the pancreas can result from an assistant's forceps compressing and retracting the pancreas, which in turn may result in pancreatic juice leakage. However, no published studies have focused on blunt trauma to the pancreas during laparoscopic surgery. Our aim was to investigate the relationship between compression of the pancreas and pancreatic juice leakage in a swine model. Materials and Methods: Three female pigs were used in this study. The pancreas was gently compressed dorsally for 15 minutes laparoscopically with gauze grasped with forceps. Pancreatic juice leakage was visualized by fluorescence imaging after topical administration of chymotrypsin-activatable fluorophore in real time. Amylase concentrations in ascites collected at specified times was measured. In addition, pancreatic tissue was fixed with formalin, and the histology of the compressed sites was evaluated. Results: Fluorescence imaging enabled visualization of pancreatic juice leaking into ascites around the pancreas. Median concentrations of pancreatic amylase in ascites increased from 46 U/L preoperatively to 12,509 U/L 4 hours after compression. Histological examination of tissues obtained 4 hours after compression revealed necrotic pancreatic acinar cells extending from the surface to deep within the pancreas and infiltration of inflammatory cells. Conclusions: Pancreatic compression by the assistant's forceps can contribute to pancreatic juice leakage. These findings will help to improve the procedure for lymph node dissection around the pancreas during laparoscopic gastrectomy.

• Journal of Gastric Cancer
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• 제24권1호
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• pp.4-28
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• 2024

Liquid biopsy, a minimally invasive procedure that causes minimal pain and complication risks to patients, has been extensively studied for cancer diagnosis and treatment. Moreover, it facilitates comprehensive quantification and serial assessment of the whole-body tumor burden. Several biosources obtained through liquid biopsy have been studied as important biomarkers for establishing early diagnosis, monitoring minimal residual disease, and predicting the prognosis and response to treatment in patients with cancer. Although the clinical application of liquid biopsy in gastric cancer is not as robust as that in other cancers, biomarker studies using liquid biopsy are being actively conducted in patients with gastric cancer. Herein, we aimed to review the role of various biosources that can be obtained from patients with gastric cancer through liquid biopsies, such as blood, saliva, gastric juice, urine, stool, peritoneal lavage fluid, and ascites, by dividing them into cellular and acellular components. In addition, we reviewed previous studies on the diagnostic, prognostic, and predictive biomarkers for gastric cancer using liquid biopsy and discussed the limitations of liquid biopsy and the challenges to overcome these limitations in patients with gastric cancer.

• 한국암석학회:학술대회논문집
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• 한국암석학회 1999년도 춘계학술대회
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• pp.13-17
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• 1999

• Journal of Gastric Cancer
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• 제13권4호
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• pp.199-206
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• 2013

Since the first transgastric natural orifice transluminal endoscopic surgery was described, various applications and modified procedures have been investigated. Transgastric natural orifice transluminal endoscopic surgery for periotoneoscopy, cholecystectomy, and appendectomy all seem viable in humans, but additional studies are required to demonstrate their benefits and roles in clinical practice. The submucosal tunneling method enhances the safety of peritoneal access and gastric closure and minimizes the risk of intraperitoneal leakage of gastric air and juice. Submucosal tunneling involves submucosal tumor resection and peroral endoscopic myotomy. Peroral endoscopic myotomy is a safe and effective treatment option for achalasia, and the most promising natural orifice transluminal endoscopic surgery procedure. Endoscopic full-thickness resection is a rapidly developing natural orifice transluminal endoscopic surgery procedure for the upper gastrointestinal tract and can be performed with a hybrid natural orifice transluminal endoscopic surgery technique (combining a laparoscopic approach) to overcome some limitations of pure natural orifice transluminal endoscopic surgery. Studies to identify the most appropriate role of endoscopic full-thickness resection are anticipated. In this article, I review the procedures of natural orifice transluminal endoscopic surgery associated with the upper gastrointestinal tract.

• 대한본초학회지
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• 제38권1호
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• pp.21-30
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• 2023

Objective : Gardeniae Fructus (GF) has bitter and cold nature. Thus, it has been traditionally prescribed in processed form roasted with ginger juice for patients with a weak stomach. This study investigated the effects of processed GF in tert-butyl hydroperoxide (tBHP)-treated gastric epithelial cells. Methods : Processed GF was made by applying 40% ginger juice or 10% ethanol for 24 h and then roasting at 150℃ for 5 minutes. Apoptosis was determined by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Mitochondrial membrane potential (MMP) was monitored by flow cytometry using the membrane permeable fluorescent dye Rh123. Protein expression was measured by Western blot analysis. Results : Cell viability was reduced by tBHP and restored by ethanol extract of GF (GFE). In the TUNEL assay, it was found that cell death by tBHP was due to apoptosis, and GFE had an anti-apoptotic effect. Processed GF roasted with ginger juice showed the best anti-apoptotic effect. Processed GF also inhibited MMP loss and restored tBHP-induced changes in expression levels of apoptosis-related proteins. Increased ROS production and GSH depletion after tBHP treatment were significantly reduced by processed GF. In addition, tBHP-induced activation of mitogen-activated protein kinase (MAPK) was inhibited by processed GF. Conclusion : These results demonstrate that the processed GF is able to protect gastric epithelial cells from oxidative stress-induced cell death with antiapoptotic and antioxidant activity. In addition, it shows that the processing of GF, which have been traditionally used for gastrointestinal protection, partially have scientific validity.

• 핵의학기술
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• 제14권2호
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• pp.17-20
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• 2010

간담도 스캔은 간세포와 담낭의 기능정도 및 담도계의 폐쇄여부를 알 수 있는 검사법이다. 또한 위부분절제술 후에 증가하는 위-담즙 역류는 담즙 역류성 위염의 주요 원인으로 알려져 있다. 본 연구는 위부분절제술 환자의 간담도 스캔에서 위-담즙역류가 관찰되어 촬영방법에 따른 역류시기와 역류지표를 알아보고자 한다. 본원 핵의학과에 2008년 10월부터 2009년 1월까지 간담도 스캔을 시행한 환자 중 위부분절제술을 한 환자 72명(남자: 52명, 여자: 20명, 연령범위: 31-77세, 평균연령: $60.5{\pm}7$세)을 대상으로 하였다. $^{99m}Tc$-mebrofenin 185 MBq/0.5 cc를 환자의 전주정맥에 주사 5분 후 바로 누운자세에서 전면상 30 kcounts를 획득하고, time setting으로 우측면상, $45^{\circ}$ 좌전사 위상을 획득하였다. 동일한 방법으로 30분, 60분, 90분, 지방식 후 120분 영상을 획득하였다. 위-담즙역류가 일어난 환자의 경우 30분 전면상, 좌전사위상에서 liver, GB, CBD의 ROI를 그리고, 이후 영상들에서 위 역류 부분의 시간 별, 자세 별 관심영역을 그렸다. 그리고 그에 따른 역류지표 구하였다. 72명의 위부분절제술 환자 중 위-담즙역류가 40명(55.6%)의 환자에서 일어났으며, 30분 영상에서 1명, 60분 영상에서 7명, 90분 영상에서 4명, 지방식 후 영상에서 28명이었다. 시간 별 위-담즙역류가 최고치는 60분 영상에서 6명, 90분 영상에서 2명, 지방식 후 영상에서 32명으로 보였다. 전면상의 역류지표 0.85-23.36%(평균: 6.53%), 좌전사위상의 역류지표 1-29.13%(평균: 8.89%)이었다. 육안평가로는 26명(65%)이 전면상보다 좌전사위상의 위-담즙역류가 더 잘 구분되어 보였다. 위부분절제술 환자의 간담도 스캔에서 위-담즙역류가 50% 이상 일어나는 것을 알 수 있고, 음식물을 섭취한 후 위-담즙역류가 가장 잘 일어나는 것을 알 수 있었다. 위-담즙역류가 일어나는 경우 장과 위의 중첩을 줄여주는 좌전사위상을 촬영하고 환자마다 그에 따른 시간대의 위-담즙 역류 지표를 제공함으로써 임상에서 간담도 스캔 목적 이외의 도움이 될 것이라 생각된다.

• Biomolecules & Therapeutics
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• 제23권5호
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• pp.458-464
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• 2015

Sennoside A (erythro) and sennoside B (threo) are dianthrone glycosides and diastereomers. We investigated their abilities to prevent the gastric lesions associated with diseases, such as, gastritis and gastric ulcer. To elucidate their gastroprotective effects, the inhibitions of $HCl{\cdot}tOH$-induced gastritis and indomethacin-induced gastric ulcers were assessed in rats. It was observed that both sennoside A and sennoside B increased prostaglandin $E_2$ ($PGE_2$) levels and inhibited $H^+/K^+$-ATPase (proton pump). In a rat model, both compounds reduced gastric juice, total acidity and increased pH, indicating that proton pump inhibition reduces gastric acid secretion. Furthermore, sennoside A and B increased $PGE_2$ in a concentration-dependent manner. In a gastric emptying and intestinal transporting rate experiment, both sennoside A and sennoside B accelerated motility. Our results thus suggest that sennoside A and sennoside B possess significant gastroprotective activities and they might be useful for the treatment of gastric disease.

• 대한약리학회지
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• 제23권2호
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• pp.87-94
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• 1987

스트레스로 인한 위궤양형성에 중추성 교감신경의 영향여부를 추구하기 위하여 norepinephrine, epinephrine, dopamine, isoproterenol 및 clonidine을 흰쥐의 뇌실내로 투여 하고 한냉 환경 $(4^{\circ}C)$에서 4시간 구속방치하여 위 분비기능의 변동과 궤양 발생 정도를 검색하여 다음과 같은 결과를 얻었다. 1. Norepinephrine, epinephrine, dopamine및 소량의 clonidine 처치로 궤양 발생이 현저하게 감소하였다. 2. Norepinephrine또는 epinephrine 처치군에서는 위액분비, 산분비 및 펩신 분비의 감소와 궤양 발생 감소가 초래되었다. 3. Dopamine혹은 소량의 clonidine 처치군에서는 궤양 발생의 감소와 위액분비 및 산분비 감소가 초래되었으나 펩신 분비는 변동 없었다. 4. Isoproterenol처치군에서는 궤양 발생과 펩신 분비는 대조군과 차이 없고, 위액분비 및 산분비의 감소만 나타났다. 5. 대량의 clonidine 투여군에서는 궤양발생, 산분비 및 펩신분비 모두 변동없이 약간의 위액분비 감소가 나타났다 이상의 결과로 보아 중추성 교감신경자극은 궤양 형성을 억압하는 작용이 있고, 이에는 교감신경성 ${\alpha}$-수용체 및 도파민성 수용체가 관여된다고 믿어지며, 이 효과는 위액분비 감소 및 산 분비 감소작용과 아울러 또 다른 요인이 관여한다고 추측된다.