• Asian-Australasian Journal of Animal Sciences
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• v.3 no.4
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• pp.281-285
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• 1990

The present paper demonstrates the expressions and amounts of pepsinogen and prochymosin in neonate, young and adult goat's proper gastric glandular regions by the immunochemical and the immunohistochemical analyses with the anti-bovine pepsinogen serum and anti-bovine chymosin serum. Each bovine serum was demonstrated to have reactivities against corresponding goat's antigen by immunochemical analses and enzymatic activities. The anti-pepsinogen was higher in the new born animals than the maternal milk feeding one, suggesting that the maternal milk might control the pepsinogen production in the proper gastric glands. The patterns of prochymosin expression in the goats was similar to that in cattle.

• Journal of Gastric Cancer
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• v.4 no.4
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• pp.207-212
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• 2004

Purpose: Invasion and metastasis in solid tumors require the action of tumor-associated proteases. The serine protease urokinase-type plasminogen (uPA) and receptor (uPAR) appear to have a major function in these processes. Expression of the uPAR is elevated in breast and colon carcinomas, and this is often associated with invasiveness and poor prognosis. The purpose of this study was to determine whether the expression of the uPAR gene correlates with clinico-pathological parameters in human gastric carcinomas. Materials and Methods: We examined the expression of uPAR mRNA by using northern blot analysis and RT-PCR in 35 gastric carcinomas and the surrounding normal mucosa. Macroscopic and histopathological tumor findings and survival rates were obtained from the patient records and from endoscopic, surgical, and pathological reports. Results: The expression of uPAR and was higher in most neoplasms than in the corresponding normal mucosal tissue. uPAR mRNA expression in tumors correlated well with lymph-node metastasis (P<0.02) and tumor stage (P<0.01). The survival rate of patients with tumors displaying high uPAR expression levels was significantly lower (P<0.04) than that of patients without uPAR expression, but IL-8 showed only the tendency of survival difference. Conclusion: These results suggest that uPAR may be an important prognostic factor in human gastric carcinomas.