• Title/Summary/Keyword: Gas Chromatography/mass spectrometry

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Volatile Components of Oyster Mushrooms (Pleurotus sp.) Cultivated in Korea (한국산 느타리 버섯류 (Pleurotus sp.)의 휘발성 향기성분)

  • Jung, Soon-Taek;Hong, Jai-Sik
    • The Korean Journal of Mycology
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    • v.19 no.4
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    • pp.299-305
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    • 1991
  • Volatile aroma concentrates of four oyster mushrooms cultivated in Korea were obtained by the simultaneous distillation-extraction method. The volatile components were identified by the combined capillary gas chromatography-mass spectrometry and compared with the of retention data on GC of those authentic compounds. About 54 volatile compounds were identified in each of the four edible oyster mushrooms. The main compounds in Pleurotus ostreatus ASI 201 were 1-octen-3-ol, 3-octanone, 3-octanol, in Pleurotus sajor-caju, 1-octen-3-ol, 3-octanone, 1,5-octadien-3-one, 3-octanol, in Pleurotus florida-ostreatus-ostreatus, 1-octen-3-ol, n-hexanol, 3-octanone, 3-octanol, phenol, and in Pleurotus ostreatus (Ae-Neutari), 1-octen-3-ol, n-pentanal, n-hexanol, n-pentanol, 3-octanone, 3-octanol, 1,5-octadien-3-one, respectively. The mushrooms worked were rich in an alcohols and carbonyl compounds containing $C_8$ compounds. The peak area ratio of $C_8$ compounds in aroma concentrates were 56.60% in Pleurotus ostreatus ASI 201, 72.46% in Pleurotus sajor-caju, 54.84% in Pleurotus florida-ostreatus-ostreatus and 35.85% in Pleurotus ostreatus (Ae-Neutari ), respectively.

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Enhancement of Bromophenol Content in Cultivated Green Grouper (Epinephelus coioides)

  • Kim, Joo-Shin;Ma, Wing Chi Joyce;Chung, Hau Yin
    • Fisheries and Aquatic Sciences
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    • v.10 no.3
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    • pp.113-118
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    • 2007
  • Bromophenols are a group of compounds found only in marine organisms. They accumulate and give a sea-like aroma to marine animals. Cultivated fishes generally contain low concentrations of bromophenols compared to wild fishes. Feeding cultivated fishes with bromophenol-containing seaweed could increase their bromophenol content and thus improve their flavor quality. We evaluated the effect of an experimental feed on the bromophenol content of green grouper, Epinephelus coioides, during an 8-week feeding period. Green grouper individuals were divided into two groups and fed with conventional feed or experimental feed containing dried seaweed. Fish were collected biweekly for 8 weeks for proximate analyses and bromophenol content evaluations. Bromophenols were extracted, identified, and quantified by gas chromatography-mass spectrometry. Both moisture and lipid contents were generally higher in the controls; however, total weight and protein content were higher in the experimental group. Only 2,4-dibromophenol and 2,4,6-tribromophenol were detected in the samples. Throughout the 8 weeks, 2,4,6-tribromophenol concentrations were higher in the experimental group (9.20-32.3 ng/g dry wt) than in the control group (7.33-18.79 ng/g dry wt), but no significant difference in 2,4-dibromophenol concentration was detected between the two groups. The total bromophenol content reached a maximum at week 4 for the experimental feed and week 6 for the control. In short, experimental feed that incorporated bromophenol-containing seaweed increased the total bromophenol content in the green grouper.

Fumigant Activity of Essential Oils and Components of Illicium verum and Schizonepeta tenuifolia Against Botrytis cinerea and Colletotrichum gloeosporioides

  • Lee, Sun-Og;Park, Il-Kwon;Choi, Gyung-Ja;Lim, He-Kyoung;Jang, Kyong-Soo;Cho, Kwang-Yun;Shin, Sang-Cheol;Kim, Jin-Cheol
    • Journal of Microbiology and Biotechnology
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    • v.17 no.9
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    • pp.1568-1572
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    • 2007
  • To develop a natural fungicide against Botrytis cinerea and Colletotrichum gloeosporioides, a total of 25 essential oils were tested for their fumigant activity against post-harvest pathogens. The vaporous phases of oils were treated to each fungus on potato dextrose agar medium in half-plate separated Petri plates at $10\;{\mu}g$ per plate. The essential oil of Illicium verum strongly inhibited the mycelial growth of both B. cinerea and C. gloeosporioides by over 90%. On the other hand, the essential oil of Schizonepeta tenuifolia showed inhibitory activity against mycelial growth of only B. cinerea by over 90%. Gas chromatography-mass spectrometry and bioassay indicated trans-anethole in I. verum and menthone in S. tenuifolia as a major antifungal constituent. The essential oils of I. verum and S. tenuifolia and their major constituents could be used to manage post-harvest diseases caused by B. cinerea and C. gloeosporioides.

Contamination Sources of Several Potentially Hazardous Compounds Found at the Gap Stream and the Miho Stream, Two Major Tributaries of the Geum River (금강 수계 주요지류인 갑천과 미호천에서 잠정유해물질 오염원 확인 연구)

  • Lee, Jun-Bae;Lee, Jay-Jung;Cho, Yoon-Hae;Yoon, Jo-Hee;Hong, Seoun-Hwa;Lee, Dae-Hee;Lee, Dae-Hee;Cho, Young-Hwan;Shin, Ho-Sang
    • Korean Journal of Environmental Agriculture
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    • v.35 no.1
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    • pp.15-23
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    • 2016
  • BACKGROUND: Water quality is of concern to water utility operators, public health officials, and populations using the water. If any contaminant is released from a point of entry, it could be spread rapidly throughout the water stream. So the identification of the location of the points of entry and its release history are critical informations to establish the management strategy.METHODS AND RESULTS: Aniline, nonylphenol, pentachlorophenol and formaldehyde in 39 surface water samples were analysed using Gas chromatography-mass spectrometry (GC-MS) methods. Formaldehyde, aniline and nonylphenol were mainly detected in the near sites where industrial waste water and domestic sewage were discharged into stream. But pentachlorophenol was detected in the downstream samples where pulp manufacturing plants were operated.CONCLUSION: Results indicate that pentachlorophenol found in main stream of Guem river was mainly introduced from pulp manufacture industries. Otherwise, formaldehyde, aniline and nonylphenol were mainly contaminated from the industrial waste water and domestic sewage.

A Study on the Identification of Hazardous Organic Substances for Industrial Classification (업종별 유해성 유기물질의 확인에 대한 연구)

  • Park, Sun Ku;Kim, Sung Soo;Ko, O Suk;Jung, Sung Woong;Park, Jun Dae;Ryu, Seung Do;Ryu, Jae Kyun;Cho, Hyun-Woo
    • Analytical Science and Technology
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    • v.9 no.4
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    • pp.392-398
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    • 1996
  • Eleven organic chemical substances, tetrachloroethylene, ethyl benzene, p-xylene, o-xylene, isopropyl benzene, n-propyl benzene. 1,2,4-trimethyl benzene, 1,3,5-trimethyl benzene, p-isopropyl toluene. see-butyl benzene, and naphthalene, which have hazardously influenced to human, were extracted from untreated wastewater collected at 26 companies of 8 types industry in the basin of Kwangju stream. Their structures were elucidated by Gas Chromatography/Mass Spectrometry(GC/MS) and in comparison with each standard reagents.

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Determination of personal care products in aquatic environmental samples by GC/MS (GC/MS를 이용한 수질환경시료 중 personal care products의 분석)

  • Lee, In-Jung;Lee, Chul-Gu;Heo, Seong-Nam;Lee, Jae-Gwan
    • Analytical Science and Technology
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    • v.23 no.5
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    • pp.477-484
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    • 2010
  • Personal care products are a diverse group of synthetic organic chemicals such as antimicrobial compounds, UV filters and organo-phosphate flame retardants and derived from individual usages of soaps, toothpaste and cosmetics. It has been detected in municipal sewage effluent and various environmental samples such as surface water, marine, soil, sediment and aquatic biota in many countries. The occurrence of personal care products in environmental samples could negatively impact the health of the ecosystem and humans, due to persistent, long-term chronic exposure of aquatic organisms. In this study, fifteen personal care products in aquatic environmental samples were determined by gas chromatography-mass spectrometry (GC-MS) with liquidliquid extraction (LLE). Method detection limits were in the range of $0.004\sim0.273\;{\mu}g/L$. Two compounds (TCEP, TCPP) were detected in surface waters and seven compounds (triclosan, 4-MBC, EHMC, BP-3, TCEP, TPP, TBEP) were detected in sewage treatment plants (STP) influents or effluents.

A Study on the Development of the Charcoal with Low Carbon Monoxide Emission using Biomass Combustion Improver (바이오매스 조연제를 이용한 CO저감형 착화탄 개발에 대한 연구)

  • Kim, Seunghee;Lee, Yeonkyung;Lee, Junseok;Jeon, Chunghwan
    • Journal of Energy Engineering
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    • v.25 no.3
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    • pp.9-17
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    • 2016
  • In this study, a method for CO reduction using char-coal combustions was developed with lignin and glycerin as combustion improvers. The relationship between CO emission and the combustion improvers was confirmed by measuring the CO concentration. The experiment to determine the combustion characteristics was conducted using glycerin, which shows high combustibility at low temperatures, impregnated with lignin, which has a specific surface area. The combustibility, volatility, and CO concentration were measured using thermo-gravimetric analysis(TGA), and gas chromatography-mass spectrometry(GC-MS). This study presents the optimal CO reduction ratio, which occurred when the combustible material contained a 20% blend of combustion improvers. This resulted in a 20-30% CO reduction rate compared to that achieved with normal char-coal.

A Survey on Benzo(a)pyrene Contamination in Amomum Tsao-ko Fruit of Medicinal Herbs (유통 한약재 초과(草果) 중 벤조피렌 오염실태 조사)

  • Whang, Kyoung-Hwa;Yeom, Mi-Sook;Lee, Hee-Jeong;Jo, A-Reum;Choi, Eun-Jeong;Heo, Myong-Je;Kwon, Mun-Ju
    • Korean Journal of Pharmacognosy
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    • v.51 no.2
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    • pp.146-150
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    • 2020
  • Amomum Tsao-ko used as a traditional oriental herbal medicine, is indigenous to several Asia countries. This study was carried out to investigate the contamination by Benzo(a)pyrene in Amomum Tsao-ko Fruit of Medicinal Herbs. 20 samples of Amomum Tsao-ko Fruit were evaluated for the Benzo(a)pyrene contamination. They were analyzed for Benzo(a)pyrene using high-performance liquid chromatogrphy(HPLC)-fluorescence detection and the positive samples were confirmed using gas chromatography tandem mass spectrometry. The levels of Benzo(a)pyrene were from 9.2 to 95.5 ㎍/kg and the average was 40.6 ㎍/kg. There are no Benzo(a)pyrene standards for Amomum Tsao-ko Fruit of Medicinal Herbs. These data will be used as a basic data for the future legislation on the regulation and control of benzo(a)pyrene of Amomum Tsao-ko Fruit of Medicinal Herbs.

Analysis of Biogenic Amines Content and the Main Volatile Flavor Compounds in Black Raspberry Wine Using Traditional Yeast (토종효모를 이용한 복분자 발효주의 바이오제닉아민 함량 및 향기성분 분석)

  • Yoon, Hae-Hoon;Son, Rak-Ho;Ryu, Eun-Hye;Jung, Ji-Hye
    • Korean journal of food and cookery science
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    • v.31 no.3
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    • pp.296-303
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    • 2015
  • We investigate black raspberry (Rubus occidentalis) wine made using traditional yeast (Saccharomyces cerevisiae A8, B6, GBY2, GBY3) and S. cerevisiae Fermivin (FM), which is widely used in wine manufacturing, and analyze the biogenic amine content and the volatile flavor compounds. Black raspberries were separately inoculated with yeast up to $1{\times}10^9CFU/kg$, followed by incubation at $25^{\circ}C$ for 7 days. FM produced the highest alcohol content, however the final fermentation characteristics of the wine made using four different yeasts were similar. S. cerevisiae A8 had a large biogenic amine (BA) content, specifically tryptamine, thus we excluded this yeast from fermentation. S. cerevisiae GBY3 was selected for black raspberry wine fermentation as a result of sensory evaluation. The volatile flavor compounds of two wines (S. cerevisiae GBY3 and FM) were analyzed by gas chromatography and mass spectrometry. 37 compounds in the samples were separated, and several ester compounds were identified in greater amounts in the wine made with S. cerevisiae GBY3 than in the wine made with FM. A greater amount of the major compound, ethyl benzoate, giving the sweet and fruity flavor, was identified in wine made with S. cerevisiae GBY3 than in the wine made with FM. In conclusion, S. cerevisiae GBY3 was confirmed to produce no major BAs and a better flavored wine. These results give new leads in the production of high quality wine.

Thermal Changes of Aroma Components in Soybean Pastes (Doenjang) (된장 가열조리 시 생성되는 향기성분 변화)

  • Lee, Seung-Joo;Ahn, Bo-Mi
    • Korean Journal of Food Science and Technology
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    • v.40 no.3
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    • pp.271-276
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    • 2008
  • In this study, volatile compounds were isolated from traditional and commercial fermented soybean pastes according to different heating temperatures (room temperature, $50^{\circ}C$, $100^{\circ}C$) using headspace-solid phase microextraction (HS-SPME). The compounds were then analyzed by gas chromatography-mass spectrometry (GC-MS). A total of 51 volatile components, including 18 esters, 3 alcohols, 6 acids, 8 pyrazines, 5 volatile phenols, 6 aldehydes, and 5 miscellaneous compounds, were identified. Esters and acids such as ethyl hexadecanoate, acetic acid, and 2/3-methyl butanoic acid were the largest groups among the quantified volatiles. By applying principal component analyses to the GCMS data sets, differences were observed in the volatile components of the soybean pastes as to the different heating temperatures. A large variation was shown between the volatile components of the traditional and commercial soybean pastes by increasing the heating temperature. Commercial samples had significantly higher levels of longer chain ethyl esters, aldehydes, and thermal degradation products such as maltol and 2-acetyl pyrrole, while traditional samples showed higher concentrations of acids and pyrazines.