• 대한본초학회지
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• 제26권4호
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• pp.155-161
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• 2011

Objective : The purpose of this study is to determine the origin and daily dose of Gardeniae Fructus in Shanghanlun. Methods : Define the origin and daily dose of Gardeniae Fructus by searching Shanhanlun and Herbology Books(本草書). And Compared these with those of current Gardeniae Fructus. Results : According to Shanhanlun, the origin of Gardeniae Fructus is Gardenia jasminoides, but G. jasminoides f. longicarpa must excluded since originally it was used for dyeing. Among the 8 prescriptions containing Gardeniae Fructus, 15 Gardeniae Fructuses for the daliy dose in Chijabekpi-tang(Zhizibopi-tang) is assumed to be 14 Gardeniae Fructuses, as it is found 14 in many other prescriptions same in other prescriptions. The quantity of 14 Gardeniae Fructuses in Shananlun is 3 Ryang(兩), which is equal to 19.5 g in the current measurement. 1 Gardeniae Fructus is equal to 1.4 g. The length of the Gardeniae Fructus in the market which is produced in korea is 22-27 mm, and the diameter is 15-19 mm, whereas in China it is 20-25 mm, and the diameter is 13-18 mm. This meets the standard requirements of the Pharmacopoeia. Conclusions : As a result from above, the origin of Gardeniae Fructus is Gardenia jasminoides, but G. jasminoides f. longicarpa must be excluded. The recommended dose in a day is 14 Gardeniae Fructuses, which is equivalent to 19.5 g, the amount generally used in Shanhanlun.

• Natural Product Sciences
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• 제19권3호
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• pp.206-214
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• 2013

The crocetin derivatives, crocin (1), gentiobiosyl glucosyl crocetin (3), and mono-gentiobiosyl crocetin (4) were isolated from the fruit of Gardenia jasminoides (Gj) and crocetin (2) from the processed fruit of Gj (PGj) by column chromatography. Their structures were determined on the basis of spectroscopic methods including IR, MS, and NMR (1D and 2D). These compounds were evaluated for their inhibition activity on melanin production in ${\alpha}$-MSH (melanocyte stimulating hormone) activated B16F10 cells. Compounds 1 - 4 reduced melanin content in a dose-dependent manner at concentrations of 20 - 60 uM. They also suppressed tyrosinase protein and m-RNA expressions dose dependently, assayed by western blot analysis, and RT-PCR experiment in B16F10 murine melanoma cells.

• Journal of Microbiology and Biotechnology
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• 제16권7호
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• pp.1084-1089
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• 2006

The fructus of Gardenia jasminoides Ellis (GF) has been widely used as a natural colorant in Asian countries, and also as a Chinese traditional medicine for its homeostatic, antiphlogistic, analgesic, and antipyretic effects. In the present study, its main component, crocin, was fermented with lactic acid bacteria (LAB) and their antihyperlipidemic activity was measured. The GF extract, fermented GF (F-GF), crocin, and fermented crocin (F-crocin) significantly inhibited the increase of serum triglyceride (TG) level in corn oil feeding-induced triglyceridemic mice, as well as that of serum TG and total and LDL cholesterol levels in Triton WR-1339-induced hyperlipidemic mice. These agents also showed hypolipidemic activity in hyperlipidemic mice induced by high fat diet for 5 weeks. F-GF and F-crocin exhibited more potent hyperlipidemic effects than GF and crocin. The results suggest that the hypolipidemic effect of GF and crocin can be synergistically activated by LAB, and that F-GF and F-crocin may improve hyperlipidemia in clinic, compared with GF and crocin.

• Journal of Microbiology and Biotechnology
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• 제1권2호
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• pp.142-149
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• 1991

Gardenia callus was induced in MS medium containing $10{\;}{\mu}M$ of 2,4 diphenoxy acetic acid (2,4-D), $1{\;}{\mu}M$ kinetin, and 3% sucrose in the dark. $B_5$ medium was identified to be the most adequate medium for cell growth. Indole-3-acetic acid (IAA) was better growth regulator than 2,4-D not only for cell growth but slso for carotenoid production. Ligt also played a critical role on synthesis of carotenoid. Gardenia cells grown in $B_5$ medium could utilize a polysaccharide, soluble starch, as a carbon source. The cell growth was stimulated in $B_5$ medium fortified with 0.2% yeast extract. The optimum pH for cell growth was 5.7. High density cultures can be maintained by increasing inoculum size and medium concentration accordingly. Specific growth rate and mass doubling time were 0.095 $day^{-1}$ and 7.3 days, respectively. The cell immobilized in alginate tends to formulate more enlarged vacuoles containing yellow pigment compared with those of suspended cell. Carotenoid content of immobilized cell was about $264.4{\;}{\mu}g/g$ fresh weight (F.W.) corresponding twice of the content of suspended cell ($112.08{\;}{\mu}g/g$ F.W.). The color of gardenia cell was shifted from yellow to red when carbohydrase-secreting fungus, Trichoderma reesei, was co-cultivated with gardenia cells.

• KSBB Journal
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• 제19권6호
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• pp.437-440
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• 2004

치자 열매 추출물로부터 미백제 개발을 위해 항산화, 항균, 항암 등 생리활성을 가지는 치자로부터 tyrosinase 효소활성 저해와 B16F10 melanoma cell을 이용한 melanogenesis 억제 효과를 실험하였다. Tyrosine을 기질로 사용하여 ethanol, hexane, chloroform, ethyl acetate 용매 및 증류수 추출물에 대한 tyrosinase 효소 저해활성을 실험한 결과 에탄올 추출물 ($81.5{\pm}1.6\%$)이 증류수 추출물 ($62.7{\pm}0.4\%$)보다 더 우수한 효과를 보여주었다. Ethyl acetate 분획물의 tyrosinase 효소활성 저해율은 다른 유기용매 분획물보다 현저히 우수한 $99.7{\pm}0.1\%$나타내었으며, 현재 tyrosinase 저해제로 개발된 arbutin ($97.8{\pm}1.2\%$)과 유사한 저해활성을 보였다. B16F10 melanoma cell을 이용한 melanogenesis 억제 효과에서도 에탄올 추출물이 증류수 추출물보다 우수한 억제활성을 보여 주었다. Ethyl acetate 분획물을 사용하였을 경우, melanogenesis 억제 효과가 다른 분획물보다 현저히 우수하였다. 이상의 실험결과는 치자 추출물의 미백원료로서 사용 가능성을 보여주었다.

• 대한본초학회지
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• 제32권2호
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• pp.97-105
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• 2017

Objectives : Gardeniae Fructus is a ripe fruit of Gardenia jasminoides Ellis, which has been used as traditional medicines for anti-inflammatory, diuretic, antipyretic, and antibacterial activity. The aim of this study was to compare of Gardeniae Fructus in South Korea collected during three years according to the standards in monographs of the Korean Pharmacopoeia Eleventh edition (KP11). Methods : 30 items of Gardeniae Fructus from two cultivation regions were classified into dried(n=15) & steamed (n=15) and tested according to the standards in monographs of the KP11. Gardeniae Fructus was carried out identification(comparison of colors, thin layer chromatography), heavy metals, residual pesticides, total ash, and assay registered at KP11. Add to we tested loss on dry, contents of ethanol-soluble extracts, and HPLC profiling. Results : In TLC chromatogram of identification test, the spot of gardenoside and geniposide were observed at $R_f$ value of about 0.3 and 0.5. Heavy metals and residual pesticides met the requirements of the standards for all samples. The results of total ash of each samples are measured maximum 4.87 %. According to HPLC for assay, the samples contain 4.80~6.10 % of geniposide and 0.45~1.83 % of gardenoside. Conclusion : We have verified the current specification standard of Gardeniae Fructus and standard that is not set. By the results, it is proposed a new draft of loss on drying and confirmed the content of gardenoside revised. HPLC standard chromatogram of Gardeniae Fructus is proposed. We hope that it will help the standardization of Gardeniae Fructus.