• The Korean Journal of Mycology
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• v.16 no.4
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• pp.247-252
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• 1988

The uptake of isolated Lyophyllum ulmarium chromosomes by Ganoderma applanatum protoplasts was induced with polyethylen $glycol+CaCl_2+glycine$. Uptake products of chromosomes by protoplasts showed micro-and macrotransgenome type. The former was slowly growing and unstable, the latter was outgrowing and stable mycelial colonies which made thick hyphae of width and segregation of mycelial colony on GCM containing benomyl. A comparison of macrotransgenome type was using isozyme analysis of esterase. The enzyme pattern of two transformants was distinct in position and quantity compared with parents.

• The Korean Journal of Mycology
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• v.17 no.4
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• pp.197-201
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• 1989

The fusion between protoplasts of Ganoderma applanatum and oidia of Lyophyllum ulmarium (Hypsizigus marmoreus) was induced with polyethylene glycol and $CaCl_2$. When transferred to Ganoderma complete medium plates, fusants showed mixed morphologies both parents. During three times subcultivation the fusants were changed similar to those of L. ulmarium type. All fusants produced oidia, clamp connections and basidiocarps similar to those of L. ulmarium. Isozyme pattern of esterase of interorder fusants showed both parental and non-parental bands. Each individual fusant did not showed both parental and non-parental bands. Each individual fusant did not show any differences in mycelial growth rate, colony morphology, esterase band pattern and basidiocarp.

• The Korean Journal of Mycology
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• v.16 no.1
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• pp.16-20
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• 1988

Auxotrophic mutants were obtained by UV-irradiation to mycelium of Ganoderma lucidum. Induction rate of auxotrophs was 5.78%. Interspecific fusion products of protoplasts were obtained by polyethylene glycol induced fusion of protoplasts from auxotrophic mutants of Ganoderma lucidum and Ganoderma applanatum. Fusion products were selected by means of the comparison with the mycelial growth rate and colony morphology. Fusion products were confirmed by mycelial morphology and esterase isozyme pattern. Some segregants were observed and fusion product produced fruit bodies.

• The Korean Journal of Mycology
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• v.17 no.3
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• pp.119-123
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• 1989

Interorder heterokaryons were obtained by polyethylene glycol induced fusion of protoplasts from auxotrophic mutants of Pleurotus ostreatus in agaricales and Ganoderma applanaturm in aphyllophorales. When transferred to MMM plates, all fusion colonies exhibited an extremely growth rate. During three times subcultivation on MCM the growth rate of fusants showed faster little by little. Seventy-five % fusion products of potoplasts showed mixed morphologies between those of P. ostreatus and G. applannatum in the first subcultivation on MCM and MGM. The phenotype of these fusants changed similar those of P. osteatus type after three times subcultivation on MCM. However, phenotype of 25% stable strains did not change on subcultivation. Hyphae of all fusion products did not form true clamp connection. All these types did not produce primordia. A comparrison of interorder somatic hybrids was made using isozyme analysis of esterase, malate dehydrogenase and peroxidase. In most cases the enzyme patterns of G. applanatum were not distinct, however, fusant showed non-parental bands.