• Title/Summary/Keyword: Gamma-irradiation

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Establishment of γ-irradiation-induced Hematopoietic Tissue Damage Model in ICR Mice (ICR 마우스에서 감마선 조사로 유도된 조혈조직 손상 모델 확립)

  • Kang, Jung Ae;Rho, Jong Kook;Jang, Beom-Su;Chung, Young-Jin;Park, Sang Hyun
    • Journal of Radiation Industry
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    • v.7 no.1
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    • pp.55-59
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    • 2013
  • Ionizing radiation causes the massive generation of reactive oxygen species, resulting in cellular and tissue damage. The present study was performed to evaluate ${\gamma}$-irradiation induced cellular damage in ICR mice. The mice were divided into four groups with ten mice in each group. Group 1 served as an unexposed control group. Groups 2, 3, and 4 were exposed to 3, 5, and 7 Gy of ${\gamma}$-radiation, respectively. Five mice per group were sacrificed 1 and 7 days after ${\gamma}$-radiation. Exposure to ${\gamma}$-irradiation resulted in hematopoietic damage in a dose-dependent manner when compared with the unexposed control group, which featured a significantly decreased spleen index. However, the exposed mice showed no significant differences in their serum AST, ALT and in the histopathological change of their liver. These results suggest that ${\gamma}$-irradiation is a good tool to prepare a hematopoietic damage model. This animal model can be employed to study the hematopoietic efficacy of biologically active compounds.

Differential Modulation of Volatile Constituents in Artemisia princeps and Artemisia argyi Plants after Gamma Ray or Electron Beam Irradiation

  • Kim, Ji Hong;Cho, Eun Ju;Lee, Min Hee;Kim, Ji Eun;Chung, Byung Yeoup;Kim, Tae Hoon;Kim, Jin-Hong
    • Journal of Radiation Industry
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    • v.6 no.1
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    • pp.17-21
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    • 2012
  • The effects of gamma ray or electron beam irradiation on herbaceous medicinal plants were investigated in terms of the composition of volatile constituents using the aerial parts or leaves of Artemisia princeps Pamp. cv. Ganghwayakssuk and Artemisia argyi cvs. Namhaeyakssuk and Hwanghaessuk. The composition of volatile constituents in leaves was clearly distinguishable among the three Artemisia cultivars. However, the relative proportions of the major volatile constituents such as 1,8-cineole, ${\alpha}$-pinene, camphene, santolina triene, and artemesia triene, were similarly changed in two or three cultivars by gamma ray or electron beam irradiation. In particular, the proportion of 1,8-cineole was increased up to 1.29- to 1.71-fold in the three cultivars after irradiation with gamma ray. These results suggest that gamma ray or electron beam irradiation can be applied to modulate the composition of volatile constituents in the leaves of Artemisia plants.

Reducing Effect of Microorganism on Meat and Fish Products by Repeated γ-Irradiation at Low Dose (저선량 감마선 반복조사의 육류와 생선 중 미생물 저감효과)

  • Je, Gil-Soo;Chung, Duck-Hwa;Shim, Won-Bo
    • Journal of Food Hygiene and Safety
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    • v.30 no.1
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    • pp.92-97
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    • 2015
  • The aim of this study is to examine the removal efficiency of pathogen (Escherichia coli O157:H7 and Salmonella typhimurium) on meat and fish products (packing condition: vacuum or not and storage temperature: $4^{\circ}C$ or $-20^{\circ}C$) repeatedly exposed at low-dose gamma irradiation. In case of meat products (beef and chicken), E. coli O157:H7 was not observed at the level of 2 kGy single gamma irradiation and 0.5 kGy repeated gamma irradiation and S. Typhimurium was not observed at the level of 2 kGy single gamma irradiation and 1 kGy repeated gamma irradiation. In case of fish products, E. coli O157:H7 and S. Typhimurium were not detected at the level of 0.5 kGy single and repeated gamma irradiation. These results showed that microorganisms on fish products were more efficiently removed than those of meat products with low-dose gamma irradiation. Generally, each packing condition made no difference. However, the products (fish and meat) stored at $-20^{\circ}C$ needed more higher dose gamma irradiation than products at $4^{\circ}C$.

Effects of Sensory Qualities of Ready-to-Eat Chicken Cooked by Different Culinary Methods and Gamma-irradiated at 10 kGy (조리 방법별 즉석 취식 닭고기의 조리 직후 감마선 조사가 관능적 품질에 미치는 영향)

  • Park, Kyung-Sook
    • Journal of Radiation Industry
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    • v.6 no.4
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    • pp.311-316
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    • 2012
  • The purpose of this study was to compare the sensory effects of different culinary methods such as electric pan cooking and charcoal roasting on gamma irradiated ready-to-eat chicken at 10 kGy. Evlauation of sensory properties were measured by estimating the organoleptic characteristics and physicochemical properties (TBARS, hardness and color different matters). The results showed that gamma irradiation after charcoal-roasting could more effectively moderate the decrease of sensory qualities and reduce off-flavor of ready-to-eat chicken than gamma irradiation after following electric pan cooking. These results suggest that gamma irradiation after charcoal broiling can be an effective culinary method for the preparation of ready-to-eat chicken.

Effect of γ-ray Irradiation on THMs Formation and Water Quality Characteristics in the Chlorination of Humic Acid Contaming Water (감마선 조사가 휴민산 염소화에 의한 THMs 생성능 및 수질특성에 미치는 영향)

  • Kang, Chul-ho;Lim, Hyun-woo;Jung, Sung-woon;Choi, Jong-hyuk;Kim, Jong-hoon;Choi, Yong-wook;Lee, Myun-joo
    • Journal of Korean Society on Water Environment
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    • v.26 no.6
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    • pp.1034-1039
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    • 2010
  • Variation of formation potential of THM (THMFP) by chlorination of humic acid and characteristics of water quality with ${\gamma}$-ray irradiation were investigated, which were divided into two categories by the order of ${\gamma}$-ray irradiation and chlorination in water treatment process. The group A consisted of the ${\gamma}$-ray irradiation followed by chlorination process of humic acid, and the group B consisted of the chlorination followed by ${\gamma}$-ray irradiation process. The pH, ORP, $UV_{254}$, and DOC decreased rapidly with an increase in ${\gamma}$-ray irradiation of 3 kGy. while conductivity was little changed. Maximum degradation ratio of chloroform in THMs of group A was 82%, while that in group B was 69%. No brominated THMs were detected at high irradiation (>3 kGy). We found that group A water treatment process was more effective in lowering the THMFP than that of group B.

Microstructural Changes in Orthopaedic-Grade Ultra High Molecular Weight Polyethylene (UHMWPE) according to Gamma-Irradiation Method (감마선 조사 방법에 따른 정형외과용 초고분자량 폴리에틸렌의 미세구조 변화)

  • Lee, Kwon-Yong
    • Polymer(Korea)
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    • v.34 no.5
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    • pp.454-458
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    • 2010
  • In this study, the microstructural changes in orthopaedic-grade ultra high molecular weight polyethylene (UHMWPE) were compartively investigated for six different gamma-irradiation methods. Compared with un-irradiation (UGI), conventional gamma-irradiation in air room temperature (AR) induced statistically significant increases of relative crystallinity and percent crosslinking in UHMWPE. Vacuum environment (VR) during gamma-irradiation significantly increased the percent crosslinking in UHMWPE. Vacuum extreme low temperature (V77) during gamma-irradiation induced no significant changes in both relative crystallinity and percent crosslinking of UHMWPE but the percent crosslinking of UHMWPE in VR and V77 was significantly larger than that in AR. Post-irradiation stabilization process significantly increased the relative crystallinity of UHMWPE in V77, and it also significantly increased the percent crosslinking of UHMWPE in AR and V77.

Physiological Responses of Rice by Acute and Chronic Gamma Irradiation (방사선 완·급조사에 따른 벼의 생리적 영향 평가)

  • Kim, Dong Sub;Song, Mira;Kim, Sun-Hee;Jang, Duk-Soo;Kang, Si-Yong;Kim, Jin-Baek;Kim, Sang Hoon;Ha, Bo-Keun
    • Journal of Radiation Industry
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    • v.5 no.1
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    • pp.55-62
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    • 2011
  • We investigated the physiological responses by acute and chronic gamma-irradiation in rice. The rice, Oryza sativa L. cv Dongan, plants were irradiated with 100 and 400 Gy of gamma-rays for acute and chronic irradiation, and their morphological, chlorophyll content, MDA, proline and activities of antioxidant enzymes were examined. The plant height of chronic irradiation samples were decrease with increase of a does than the control, but the number of tiller and dry weight of shoot were increased 100 Gy. Carotenoid and chrolorphyll content were decreased of all irradiated plants than non-irradiated plants. But, MDA and proline content were increased in 400 Gy both acute and chronic gamma irradiation. And the activities of antioxidant enzymes were different as gamma-irradiation patterns.

Effects of Gamma Irradiation on Pigments of Beef (감마선 조사가 한우육의 색소에 미치는 영향)

  • Yook, Hong-Sun;Lee, Ju-Woon;Lee, Kyong-Haeng;Kim, Sung;Byun, Myung-Woo
    • Korean Journal of Food Science and Technology
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    • v.30 no.5
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    • pp.1184-1188
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    • 1998
  • Effects of gamma irradiation on pigments of beef was investigated by determination of the Hunter's color values and pigments such as myoglobin, metmyoglobin and oxymyoglobin after gamma irradiation up to 5 kGy in air or vacuum package for 7 days. 'L' and 'b' values increased but 'a' value decreased with the elapse of the storage period. But, 'L', 'a' and 'b' values of irradiated beef were lower than non-irradiated beef. Myoglobin decreased but oxymyoglobin and metmyoglobin increased with the elapse of the storage period. Myoglobin decreased but oxymyoglobin and metmyoglobin increased with gamma irradiation up to 5 kGy. In regard to packaging methods, vacuum package did not affect pigment of the beef as did air package.

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Effect of Electron Beam and ${\gamma}$-Ray Irradiation on the Curing of Epoxy Resin

  • Kang, Phil-Hyun;Park, Jong-Seok;Nho, Young-Chang
    • Macromolecular Research
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    • v.10 no.6
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    • pp.332-338
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    • 2002
  • The effect of an electron beam and ${\gamma}$-ray irradiation on the curing of epoxy resins was investigated. Diglycidyl ether of bisphenol A (DGEBA) and diglycidyl ether of bisphenol F (DGEBF) as epoxy resin were used. The epoxy resins containing 1.0-3.() wt% of triarylsulphonium hexafluoroantimonate(TASHFA) and triarylsulphonium hexafluorophosphate(TASHFP) as initiator were irradiated under nitrogen at room temperature with different dosage of EB and ${\gamma}$-rays from a Co$^{60}$ u source. The chemical and mechanical characteristics of irradiated epoxy resins were compared after curing of EB and ${\gamma}$-ray irradiation. The thermal properties of cured epoxy were investigated using dynamic mechanical thermal analysis. The chemical structures of cured epoxy were characterized using near infrared spectroscopy. Mechanical properties such as flexural strength, modulus were measured. The gel fraction of DGEBA with ${\gamma}$-ray was higher than that of the epoxy with EB at the same dose. Young's modulus of the sample irradiated by ${\gamma}$-ray is higher than that of sample cured by EB. From the result of strain at yield, it was found that the epoxy cured by ${\gamma}$-ray had a higher stiff property compared with the irradiated by EB.

Effects of ${\gamma}$-Irradiation on Immunological Activities of ${\beta}$-Glucan

  • Kim, Jae-Hun;Sung, Nak-Yun;Byun, Eui-Hong;Kwon, Sun-Kyu;Song, Beom-Seok;Choi, Jong-Il;Yoon, Yohan;Kim, Jin-Kyu;Byun, Myung-Woo;Lee, Ju-Woon
    • Food Science and Biotechnology
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    • v.18 no.5
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    • pp.1305-1309
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    • 2009
  • This study evaluated the effects of $\gamma$-irradiation on immunomodulating properties and structural changes of ${\beta}$-glucan. ${\beta}$-Glucan solutions (10 mg/mL) were ${\gamma}$-irradiated at 10, 30, and 50 kGy. Splenocyte proliferation and cytokine (interferon-${\gamma}$ and interlukin-2) productions by ${\gamma}$-irradiated ${\beta}$-glucan were evaluated in in vivo and in vitro, and structural changes of ${\beta}$-glucan were also determined after ${\gamma}$-irradiation. ${\gamma}$-Irradiation on ${\beta}$-glucan at 50 kGy enhanced splenocyte proliferation and cytokine productions, (p<0.05) and cleft glycosidic bonds of ${\beta}$-glucan resulting in lower the molecular weight. These results indicate that the use of ${\gamma}$-irradiation on ${\beta}$-glucan may be useful for improving its immunological activity by lowering the molecular weight of ${\beta}$-glucan.