• Asian-Australasian Journal of Animal Sciences
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• v.22 no.8
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• pp.1085-1090
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• 2009

There are currently five primary breeds of Chinese gamecock, the Henan, Luxi, Tulufan, Xishuangbanna andZhangzhou. Though there is historical evidence of cockfighting in China dating as far back as 2,800 years, the origin and genetic relationships of these breeds are not well understood. We used sequence variation from the mtDNA cytb gene and control region (1,697 bp) to examine the domestication history and genetic relationship of the Chinese gamecock. From 75 samples (14-16 per breed) we found 34 haplotypes, and 45 variable nucleotides. Phylogenetic reconstruction indicated multiple origins of the gamecock breeds. The breeds in the north and center of China, Tulufan, Luxi and Henan, clustered together in a haplogroup and may have the same ancestor. However the southern breeds, Zhangzhou and Xishuangbanna clustered into two isolated haplogroups, suggesting another two origins of Chinese gamecock. Meanwhile, extensive admixture was also found because samples from different breeds, more or less, were always grouped together in the same clades. Based on these results, we discuss the possibilities of multiple origins of gamecock breeds, from both ancestral gamecocks as well as other domestic chickens and red jungle fowl.

• Journal of Microbiology and Biotechnology
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• v.32 no.3
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• pp.355-364
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• 2022

From independent swab samples of the cloaca of indigenous gamecocks (CIG), anus of healthy baby goats (AHG), and vagina of goats (VG) originating from Phitsanulok, Thailand, a total of 263 isolates of lactic acid bacteria (LAB) were collected. Only three isolates, designated C707, G502, and V202, isolated from CIG, AHG, and VG, respectively, exhibited an excellent inhibitory zone diameter against foodborne pathogenic bacteria when evaluated by agar spot test. Isolates C707 and G502 were identified as Enterococcus faecium, whereas V202 was identified as Pediococcus acidilactici, based on 16S rRNA sequence analysis. When foodborne pathogenic bacteria were co-cultured with chosen LAB in mixed BHI-MRS broth at 39℃, their growth was suppressed. These LAB were found to be capable of surviving in simulated stomach conditions. Only the isolate G502 was able to survive in the conditions of simulated intestinal juice. This research suggests that selected LAB could be used as a food/feed supplement to reduce foodborne pathogenic bacteria and improve the safety of animal-based food or feed.