• Title/Summary/Keyword: Galleria mellonella L.

Search Result 22, Processing Time 0.084 seconds

The Detection and Multiplicity of Entomopathogenic Nematodes Using Silkworms (Bombyx mori) (누에를 이용한 곤충병원성 선충의 검색 및 배양)

  • 한상미;이광길;여주홍;권해용;우순옥;이용우;백하주;한명세
    • Journal of Sericultural and Entomological Science
    • /
    • v.45 no.2
    • /
    • pp.103-107
    • /
    • 2003
  • Entomopathogenic nematodes were isolated through the investigation in soils collected from cultivated and non-cultivated fields using silkworms (Bombyx mori) and Galleria mellonella trap. The detectable rate of entomopathogenic nematodes of silkworms trap was higher than the G. mellonella trap. This study indicates the detection of entomopathogenic nematodes from soils that silkworms are sensitive superior to the G. mellonella to entomopathogenic nematodes. The steinernema, rhabditidae, and diplogatroidae strains successfully cultured on the silkworms host as well as on artificial media. Reproductivity in the living silkworm larva and pupa was 1.5 to 3.5${\times}$ 10$\^$5/ nematodes per host However, G. mellonella could be multiplied less than 5${\times}$l0$\^$5/ nematodes. The dried pupa of the silkworm following mositurize was cultured 0.5 to 2${\times}$10$\^$5/) nematodes per host. The culture methods of the steinernema, rhabditidae, and diplogatroidae strains, using silkworm powder, extracted chicken intestine, and food waste fertilizer could be applicative, but rate of reproduction was low.

Changes in the Haemolymph Metabolites, Protein, Carbohy-drate, and Free Amino Acid of Gallena mellonella L. Parasi-tized by the Pupal Parasitoid, Brachymeda lasus Walker (무늬수중다리좀벌(Brachymeda lasus Walker)에 의해 기생된 꿀벌부채명나방(Gallena mellonella L.) 번데기의 혈림프 대사물질에서 단백질, 탄수화물, 유리아미노산의 변화)

  • 이해풍;고태용;이경로
    • The Korean Journal of Zoology
    • /
    • v.33 no.2
    • /
    • pp.166-174
    • /
    • 1990
  • Gallena mellonella L. was parasitized by the pupal parasitoid, Brachymerica lasus Walker in the laboratory. Haemolymph total protein and carbohydrate concentrations from the hosts parasitized by B. lasus decreased within 1st day following parasitization. However, haemolymph total free amino adds, glycine, proline, arginine, isoleucine, phenylalanie, leucine, valine, glutamk acid, histidine, serine, and tyrosine increased and free amino acids decreased were threonine, aspartic acid, methionine, cysteine, alanine, and lysine.

  • PDF

Liquid culture of entomopathogenic nematodes

  • Park, Seon-Ho;Yu, Yeon-Su
    • 한국생물공학회:학술대회논문집
    • /
    • 2000.11a
    • /
    • pp.443-444
    • /
    • 2000
  • An in vitro liquid culture media for the cultivation of entomopathogenic nematode Steinernema carpocapsae was developed. Supplementation of whole milk powder with basal liquid culture media showed a remarkable increase in productivity compared to that without whole milk powder and the maximum nematode concentration reached about $1.5{\times}10^5/mL$ within 20 days. Five to twenty gram per liter of liver extract addition revealed highest pathogenicity against 3rd instar of Galleria mellonella which was above about 90% mortality after 48 hr.

  • PDF