• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.spc1
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• pp.136-140
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• 2005

This study was conducted to determine antioxidative enzyme activity in serum of rats as affected by soybean cultivar, extract feeding concentration and fat dietary. In all cultivars, activities of antioxidant enzymes in treatment of various fat compositions and cholesterol followed by feeding soy-extracts were higher than in nonfeeding control, however, no significant differences existed in statistically. The activities of SOD and GSHpx in serum of rats when fed with various fat dietary were higher in Geomjeongkong 1 than in Tawonkong and Hwangkeumkong. Enzyme activities of SOD and GSHpx with each fat compositions in treatment soy-extracts was no significant differences statistically. There was significant differences among cultivars and concentrations of soy-extracts in interaction effect. Also, the activities of GSHpx was significant differences among cultivars and concentrations of soy-extracts and fat composition in interaction effect.

• Journal of the Korean Society of Food Science and Nutrition
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• v.23 no.3
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• pp.429-435
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• 1994

The effect of methionine (Met) supplementation on glutathione peroxidase(GSHPx) activity in young and 14 month-old rat and mice was investigated. GSHPx activity was more enhanced by methionine supplementation in young rats when selenium (Se) was given as selenite than given in the form of selenomethione (Se-Met). However, GSHPx activity was not influenced by Met supplementation in the old rats. When diets were low in Se, the biopotency of ht eenzyme by Met was facilitated. No significant differences in GSHPx activity was observed with Met supplement in growing mice when Met was given 0.3% and 0.8% iin the diet at high levels of Se (2 ppm). The peak GSHPx in liver and kidney occurred at day 18, thereafter it decreased. Particularly, the liver GSHPx at day 18 increased 4.2 times than that at day 4 by 0.5% Met supplementation, while the unsupplemented group remained only 2.5 times increase. It is considered that in some tissues Met requirement may be met by Se-Met when rats were fed a diet suboptimal in Met. In addition, at lower levels of Se the utilization of Se is more enhanced by Met than at higher levels of dietary Se. Therefore, GSHPx activity may be influenced greatly by Met status along with dietary Se.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.4
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• pp.501-507
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• 2010

Three hundred chicks were randomly assigned among four treatments to evaluate the effect of an organic selenium supplement at various levels of dietary protein. Two levels of supplemental selenium (0 and 0.3 mg/kg) from zinc-L-selenomethionine were tested at two levels of dietary protein (normal and reduced) in a completely randomized design with $2{\times}2$ factorial layout. The experiment lasted up to 49 d of age during which all birds were exposed to $31{\pm}1^{\circ}C$. The effects of selenium or its interaction with CP on growth performance and carcass characteristics were not significant. However, feeding the reduced-CP diet decreased weight gain in the starting period and increased liver and abdominal fat weights relative to body weight. Ferric reducing ability of plasma (FRAP) was not significantly affected by dietary CP and Se or their interaction though FRAP values were numerically higher in the Se-supplemented group. Dietary CP content did not affect the activity of plasma glutathione peroxidase (GSHPx), though Se significantly elevated plasma GSHPx activity. The interaction of CP and Se was not significant for FRAP and plasma GSHPx activity.

• Journal of Life Science
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• v.7 no.1
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• pp.10-16
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• 1997

This study was designed as a part of efforts to investigate the biochemical pollutant markers for diagnosis of marine pollutions by changes in oxygen radicals and their scavenger enzymes of the flounder (Paralichthys olivaceus)in Yellow Sea of Kores. Protein contents in brian and muscle of cultured flounder in Yellow Sea were remarkably lower(30-45% and 25-45%, respectively) than those of wild flounders in Pohang(control) of East Sea. Lipid peroxide(LPO) levels in serum of cultured and wild flounders in Yellow Sea were significanltly higher (30-80% and 125-145%, respectively)than those of wild flounder in Pohang. Hydroxide radical formations and superoxide dismutase(SOD) activities in serum of cultured flounders in Yellow Sea were significantly 15-30% and 15-35% lower than those of wild flounders in Pohang, but glutathione peroxidase (GSHPx) activities in brain of cultured flounders in Yellow Sea were significantly 15-25% higher than those of wild flounders in Pohang. It is believed that significantly decreases of protein contents in brain anad muscle, remakable increases of malondialdehyde(LPO) in serum and glutathione peroxidase (GSHPx)in brain of cultured flounders of Yellow Sea may be used as a biochemical pollutant markers for diagnosis of marine pollutions.

• Journal of Nutrition and Health
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• v.27 no.9
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• pp.940-948
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• 1994

The present study was designed to determine if prenatal and postnatal Se nutriture affects Se concentration, glutathione peroxidase(GSHPx) activity and phospholipid distribution of the neonatal rat lung. Female SD rats were bred and fed a semipurified Se-deficient(0.04ppm, Se-) or a Se-adequate(0.5ppm, Se+) diet through pregnancy and lactation. On d 2 of lactation, maternal dietary Se had no significant effect on pulmonary Se concentration of pups. On d 16 of lactation, mean milk Se concentration in Se- dams was significantly lower than that in Se+ dams. Milk Se concentration was reflected on lung Se concentration and GSHPx activity of d 16 pups, which were dramatically decreased in Se- pups. In addition, pulmonary disaturated phosphatidyl choline/total phosphatidyl choline ratio was also significantly decreased in Se- pups, implying impaired function of pulmonary surfactant. These data indicate that adequate Se nutrition is important in the maturation of neonatal rat lungs.

• BMB Reports
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• v.39 no.6
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• pp.656-661
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• 2006

Cisplatin (CDDP) is a widely used anticancer drug, but at high dose, it can produce undesirable side effects such as hepatotoxicity. Because silymrin has been used to treat liver disorders, the protective effect of silymarin on CDDP -induced hepatotoxicity was evaluated in rats. Hepatotoxicity was determined by changes in serum alanine aminotransferase [ALT] and aspartate aminotransferase [AST], nitric oxide [NO] levels, albumin and calcium levels, and superoxide dismutase [SOD], glutathione peroxidase [GSHPx] activities, glutathione content, malondialdehyde [MDA] and nitric oxide [NO] levels in liver tissue of rats. Male albino rats were divided into four groups, 10 rats in each. In the control group, rats were injected i.p. with 0.2 ml of propylene glycol in saline 75/25 (v/v) for 5 consecutive days [Silymarin was dissolved in 0.2 ml of propylene glycol in saline 75/25 v/v]. The second group were injected with CDDP (7.5 mg /kg, I.P.), whereas animals in the third group were i.p. injected with silymarin at a dose of 100 mg/kg/day for 5 consecutive days. The Fourth group received a daily i.p. injection of silymarin (100 mg/kg/day for 5 days) 1 hr before a single i.p. injection of CDDP (7.5 mg/kg). CDDP hepatotoxicity was manifested biochemically by an increase in serum ALT and AST, elevation of MDA and NO in liver tissues as well as a decrease in GSH and the activities of antioxidant enzymes, including SOD, GSHPx in liver tissues. In addition, marked decrease in serum NO, albumin and calcium levels were observed. Serum ALT, AST, liver NO level, MDA was found to decreased in the combination group in comparison with the CDDP group. The activities of SOD, GSHPx, GSH and serum NO were lower in CDDP group than both the control and CDDP pretreated with silymarin groups. The results obtained suggested that silymarin significantly attenuated the hepatotoxicity as an indirect target of CDDP in an animal model of CDDP-induced nephrotoxicity.

• Korean Journal of Veterinary Research
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• v.39 no.4
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• pp.679-688
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• 1999

The combined effects of iron and selenium status on glutathione peroxidase (GSHPx) activity, cytochrome P-450 activity, and lipid peroxidation in the liver and intestinal mucosa of rats were investigated. In experiment one, four experimental groups (+Se+Fe, -Se+Fe, +Se++Fe, -Se++Fe) were manipulated for 3 weeks with intramuscular administration of irondextran (++Fe) and/or normal diet (+Fe) and deionized water (-Se) and/or selenium-supplemented deionized water (+Se). In experiment two, 2% dietary carbonyl iron (instead of the parenteral administration) was fed for 3 weeks to rats. Body weight of rats was significantly decreased in both parenterally and orally iron-overloaded groups (p<0.01), regardless of Se supplement. Serum iron was significantly increased in parenterally iron-overloaded groups but it was marginally increased in orally iron-overloaded groups. There was no significant difference in hemoglobin content among experimental groups in either experiment one or two. Total iron in the small intestine, intestinal mucosa, and livers was significantly high in both parenterally and orally iron-overloaded rats, regardless of selenium status. In the liver and intestine, GSHPx activity was significantly higher in all selenium-supplemented groups, compared to Se-deficient groups (p<0.01) and lipid peroxidation was significantly enhanced in both parenterally and orally iron-overloaded groups, compared to iron-adequate groups. There was no significant difference in cytochrome P-450 activity in the livers between groups in both experiment one and two. These results indicated that GSHPx activity in liver and intestinal mucosa was depended on selenium status, regardless of iron status, and iron-overload enhances lipid peroxidation in liver and intestinal mucosa by increasing the tissue iron content.

• Journal of Nutrition and Health
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• v.33 no.5
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• pp.507-516
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• 2000

The purpose of this study was to investigate the effect of vitamin C and vitamin E supplementation on the iron contents and oxidative stress of the rats. Rats were fed 18g ascorbic acid and 300IU $\alpha$-tocopherol/kg diet, respectively. Rats were sacrificed at 1, 3, 5 and 7 month of age. The blood, liver and brain were selected for the quantitation of iron and malondialdehyde(MDA) contents, glutathione peroxidase(GSHPx), superoxided dismutase(SOD) and catalase(CAT) activity. Iron and MDA contents and GSHPx activities were increased with aging. Vitamin C and Vitamin E supplementation increased iron contents of the plasma. Vitamin C raised iron contents, but vitamin E decreased iron contents of the liver. In the brain vitamin C and vitamin E did not affect the iron level. MDA levels were decreased with vitamin C and vitamin E supplementation in the erythrocyte and liver, and vitamin C supplementation elevated MDA levels in the brain. GSHPx activity was increased with vitamin C and vitamin E supplementation. SOD activities of erythroucyte and brain were not affected with age, but in the liver, SOD activity was raised with age and vitamin C supplementation. Vitamin C and vitamin E supplementation promoted CAT activity of erythroucyte and liver, and CAT activity of brain was eleveated with vitamin addition but was decreaed with vitamin E addition. Vitamin C and vitamin E decreased iron contents of blood plasma, MDA contents of plasma and liver, and CAT activity of erythrocyte. Above results indicated that iron contents and biomarkers of oxidative stress were more affected by age than antioxidant action of vitamin C and vitamin E.

• Animal Bioscience
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• v.35 no.5
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• pp.721-729
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• 2022

Objective: An experiment was conducted to determine the effects of supplementing graded concentrations of inorganic sulphur (S) without and with folic acid (FA) in maize-soybean meal diets on performance, slaughter and anti-oxidant variables, immune responses and serum protein fractions in broiler chicken. Methods: Inorganic S was supplemented at 0.05%, 0.10%, 0.15%, and 0.20% alone or in combination with FA (4 mg/kg) in basal diet (BD) containing no supplemental methionine (Met) and FA. A control group was fed with the recommended concentration of Met. Each diet was offered to 10 pens of 5 male broiler chicks (Cobb 400) and fed ad libitum from day 1 to 42. Results: The broilers fed the BD had lower body weight gain (BWG), feed efficiency (FE), higher lipid peroxidation (LP), lower activity of glutathione peroxidase (GSHPx), lower lymphocyte proliferation ratio (LPR), and reduced concentrations of total protein, albumin, and globulin in serum. Supplementation of FA and S to the BD improved the BWG (all concentrations of S) and FE (0.20% S) similar to the control group. Similarly, the combination of S and FA significantly improved the concentrations of total protein, albumin, and globulin in serum, reduced the LP and increased the activity of GSHPx and LPR. However, responses in the above parameters were related to the concentration of S in the diet. The slaughter variables and antibody titres against the Newcastle disease were not affected with the treatments. Conclusion: Based on the results, it is concluded that the combination of S (0.2%) and FA (4 mg/kg) improved the BWG and FE, similarly supplementation of these nutrients improved the concentration of protein fractions and reduced the stress (reduced LP and improved GSHPx) variables in serum and improved the cell mediated immune response (LPR) in broilers fed sub-optimal concentrations of Met in diet.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.4
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• pp.608-613
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• 1997

This study was designed to investigate the biochemical pollutant marker for diagnosis of marine pollutions by changes in oxygen radicals and their scavenger enzymes of the flounder (Pleuronichthys cornutus) in the Yellow Sea of Korea Protein contents in brain and muscle of wild flounders in the Yellow Sea were remarkably lower $(15\~45\%,\;and\;35\~45\%,\;respectively)$ than those of wild flounder in Pohang (control) of the East Sea. Lipid peroxide (LPO) levels in serum of wild flounders in the Yellow Sea were Significantly higher $(30\~70\%)$ than those of wild flounder in Pohang. Hydroxyl radical formations in serum of wild flounders in the Yellow Sea were significantly high $(15\~90\%)$ than those of wild flounders in Pohang. Superoxide dismutase (SOD) activities in serum of wild flounders in the yellow Sea were significantly lower $(20\~40\%)$ than those of wild flounders in Pohang, and glutathione peroxidase (GSHPx) activities in brain of wild flounders in the Yellow Sea were also significanlty lower $(10\~60\%)$ than those of wild flounders in Pohang. These results suggest that significantly decreases of protein contents in brain and muscle, remarkable in creases of malondialdehyde (LPD) in serum and decreases of SOD and GSHPx activities in serum and brain of wild flounders of the Yellow Sea may be used as a biochemical pollutant markers for diagnosis of marine pollutions.