• Proceedings of the Korean Society of Crop Science Conference
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• 2019.09a
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• pp.22-22
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• 2019

Green stem disorder (GSD) of soybean (Glycine max (L.) Merr.) is characterized by delayed senescence of stems with normal pod ripening and seed maturation (Hobbs, 2006). GSD complicates harvesting of soybeans by significantly increasing the difficulty in cutting the affected plants. There is also the potential for moisture in the stems to be scattered on the seed, reducing the grade and storability of the seed. Not only the cause of GSD is yet unknown, but also GSD cannot be evaluated until maturity, therefore the method to evaluate GSD in early growth stage with high sensitivity is necessary. In previous studies, it has been reported that vegetative storage protein (VSP) accumulates and the syndrome of GSD appears in soybean after depod treatment (Fischer, 1999). Soybean VSP is a storage protein which is abundant in young sink leaves and degraded during seed fill (Wittenbach, 1982). Hence, we have established a system to quantify VSP of high sensitivity by using standard protein made by genetically transformed E. coli and specific antibody against VSP, and studied the relationship between VSP and GSD, by depod experiment and drought/excess wet experiments. The result of depod experiment with the cultivar 'Yukihomare' was the same with the previous studies, VSP accumulated much more than control and the syndrome of GSD appeared in soybean in depod treatment. Drought and excess wet had different impact on GSD. Excess wet caused GSD of the cultivar 'Tachinagaha (GSD susceptible)', while drought caused a little syndrome of GSD in the cultivar 'Touhoku 129 (GSD resistant)'. The accumulation of VSP differed between the two cultivars over time. In conclusion, the accumulation of VSP came along with the emergence of GSD. Different cultivars showed different response to drought and excess wet. In the future, it is expected that the dynamics of VSP will be elucidated in detail, leading to the development of early diagnosis technology for green stem disorder and the elucidation of mechanism of soybean GSD.

• The Korea Journal of Herbology
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• v.29 no.6
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• pp.55-62
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• 2014

Objectives : The objective of this study is to determine the best numbers of steaming-drying cycles of Gastrodiae Rhizoma for both efficacy and taste. We investigated various characteristics among Gastrodiae Rhizoma samples (GSD1, GSD3, GSD5 and GSD7) through the number of steaming-drying cycles increased. Methods : Gastrodiae Rhizoma were steamed and dried at different repeated numbers. They were divided into samples (GSD1; steamed and dried once, GSD3; steamed and dried three times, GSD5; steamed and dried five times, GSD7; steamed and dried seven times) for experiment. They were extracted using water, freeze dried and powdered to analyze proximate composition, free sugar amount, functional components, antioxidant activity and sensory evaluation. Results : Proximate composition and the amount of free sugars of Gastrodiae Rhizoma did not have meaningful differences among samples. Phenolic and flavonoid content of samples were increased by increasing steaming-drying numbers. Gastrodin content had different values and GSD7 was the highest in comparison with others. Increasing steaming-drying numbers led to a increasing in radical and nitrate scavenging activity in samples. Regarding to sensory evaluation, GSD5 was selected as the best sample according to its highest hedonic score mean (5.54/7) among all samples for appearance, color, taste and overall acceptability. Conclusions : The results indicated that 5th-cycling sample was effective in views of functional components, antioxidant activity and sensory characteristics. Moreover, it was suggested that steaming-drying process improved remarkably the effects of Gastrodiae Rhizoma.

• Journal of Internet Computing and Services
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• v.8 no.5
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• pp.45-58
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• 2007

This paper presents UI4GSD (User Interface for Grid Service Development) for grid service developers that provides an efficient and friendly development environment based on Globus Toolkit 4, Normally, implementing grid service requires special expert knowledge for Grid as well as programming, Moreover, grid service development as well as testing of the deployed service in the Globus container takes a long Time, which makes the grid service implementation very inefficient, However, UI4GSD can automatically generate a grid service interface file, a build file, and grid service class files as well as a client class file, using the information supplied by developers through GUI. In UI4GSD, a grid service is developed easily based on typical five step processes with required input data fed at each step. As, a result, UI4GSD can provide an easy and convenient development environment thereby increasing the efficiency and convenience in developing grid services.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.4 no.2
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• pp.213-217
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• 2001

Purpose: Glycogen storage disease type Ia (GSD Ia) is an autosomal recessive disorder of glycogen metabolism caused by glucose-6-phosphatase (G6Pase) deficiency. The clinical manifestations of G6Pase deficiency include growth retardation, hepatomegaly, hypoglycemia, lactic acidemia, hyperlipidemia and hyperuricemia. Many mutations of this gene have been found worldwide in various ethnic groups, establishing the molecular basis of GSD Ia. To elucidate a spectrum of the G6Pase gene mutations in Korean, we analyzed mutations in Korean patients with GSD Ia. Methods: Both alleles of 9 unrelated GSD 1a patients were studied by PCR and direct DNA sequencing methods. In all patients, GSD 1a was diagnosed by the enzyme assay for the liver biopsy specimen. Results: In Korean, the most prevalent mutation was g727t substitution in exon 5, which has been reported to cause abnormal mRNA splicing: Sixteen out of 18 alleles were found to have this mutation. In addition, we identified one novel mutation, a c611g, converting a proline to an alanine at codon 178. Conclusion: Our findings suggest that a screening for the g727t mutation by noninvasive molecular method can detect most cases of GSD Ia in Korean patients.

• Soft Computing and Machine Intelligence
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• v.1 no.1
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• pp.36-52
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• 2021

Software development organizations are globalizing their development activities increasingly due to strategic and economic gains. Global software development (GSD) is an intricate concept, and various challenges are associated with it, specifically related to the software requirement change management Process (RCM). This research aims to identify humans' related success factors (HSFs) and human-related challenges (HCHs) that could influence the RCM process in GSD organizations and propose a theoretical framework of the identified factors concerning RCM process implementation. The Systematic Literature Review (SLR) method was adopted to investigate the HSFs and HCHs. Using the SLR approach, a total of 10 SFs and 10 CHs were identified. The study also reported the critical success factors (HCSFs) and critical challenges (HCCHs) for RCM process implementation following the factors having a frequency 50% as critical. Our results reveal that five out of ten HSFs and 4 out of ten HCHs are critical for RCM process implementation in GSD. Finally, we have developed a theoretical framework based on the identified factors that indicated a relationship among the identified factors and the implementation of the RCM process in the context of GSD. We believe that the results of this research can help tackle the complications associated with the RCM in GSD environment, which is vigorous to the success and progression of GSD organizations.

• Journal of Microbiology and Biotechnology
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• v.34 no.2
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• pp.262-269
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• 2024

Panax ginseng has been widely applied as an important herb in traditional medicine to treat numerous human disorders. However, the inflammatory regulation effect of P. ginseng distillate (GSD) has not yet been fully assessed. To determine whether GSD can ameliorate inflammatory processes, a GSD was prepared using the vacuum distillation process for the first time, and the regulation effect on lipopolysaccharide-induced macrophages was assessed. The results showed that GSD effectively inhibited nitric oxide (NO) formation and activation of inducible nitric oxide synthase (iNOS) mRNA in murine macrophage cell, but not cyclooxygenase-2 production. The mRNA expression pattern of tumor necrosis factor alpha and IL-6 were also reduced by GSD. Furthermore, we confirmed that GSD exerted its anti-inflammatory effects by downregulating c-Jun NH₂-terminal kinase (JNK) phosphorylation, the extracellular signal-regulated kinase phosphorylation, and signaling pathway of nuclear factor kappa B (NF-κB). Our findings revealed that the inflammatory regulation activity of GSD could be induced by iNOS and NO formation inhibition mediated by regulation of nuclear factor kappa B and p38/JNK MAPK pathways.

• Journal of Life Science
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• v.30 no.8
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• pp.672-679
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• 2020

Distal myopathy is a clinically and genetically heterogeneous group of degenerative diseases of the distal muscle. Glycogen storage disease type IXD (GSD9D) is a metabolic distal myopathy characterized by muscle deficiency of phosphorylase kinase, a key regulatory enzyme in glycogen metabolism. Affected individuals may develop muscle weakness, degeneration, and cramps, as well as abnormal muscle pain and stiffness after exercise. It has been reported that mutations in the PHKA1 gene which encodes the alpha subunit of muscle phosphorylase kinase cause GSD9D. In this study, we examined a Korean GSD9D family with a c.3314T>C (p.I1105T) mutation in the PHKA1 gene. This mutation has not been previously reported in any mutation database nor was it found in 500 healthy controls. The mutation region is well conserved in various other species, and in silico analysis predicts that it is likely to be pathogenic. To date, only seven mutations in the PHKA1 gene have been documented, and this is the first report of Korean GSD9D patients. This study also describes and compares the clinical symptoms and pathological conditions of previously reported cases and these Korean patients. We believe that our findings will be useful for the molecular diagnosis of GSD9D.

• Journal of Korean Society for Geospatial Information Science
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• v.18 no.1
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• pp.135-143
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• 2010

The national base map has its value sand roles as the basic spatial information of the nation. The current national base map that is a 1/5,000 digital map, however, has failed to perform its roles as basic spatial information due to the limitations with its quality and accuracy and requires measures to complement them. Thus this study set out to suggest ways to advance the current 1/5,000 national base map, selected topography and natural features of a digital map that could be made with GSD 0.25m digital aerial photographs, and set up the optimal ways to make a digital map by conducting an experiment of making an optimal digital map with such photographs. It also analyzed the map made with GSD 0.25m digital aerial photographs for accuracy and usability. In order to establish a set of criteria of making a digital map with GSD 0.25m digital aerial photographs, the investigator carried out analyses and picked topography and natural features items, which include 9 large categories, 31 medium categories, and 509 small categories. Then an experiment of making a digital map was conducted according to the digital map making method. As a result, solid drawing was selected as the optimal way to making a digital map, and the optimal process was established. Using the research achievements, a model digital map was made with GSD 0.25mm digital aerial photographs. The map recorded about two times horizontal and vertical location accuracy than the old 1/5,000 digital map and was capable of detailed descriptions of topography and natural features. A new national base map made with GSD 0.25m digital aerial photographs will provide reliable spatial data, thus increasing the level of satisfaction among people and the level of advancement of national base maps.

• Korean Institute of Interior Design Journal
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• v.13 no.2
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• pp.192-199
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• 2004

This study is to understand the emotional tendency through out the history of architecture and interior design. In this process, a broad range of analysis of the period between Romanticism and Post Modernism will be done to collect historical data and the influence of the social situation. A thorough review and close examination of the 90's architectural trend will reveal the foundation of the concept behind GSD not just as the post-structuralism as a value system of digitalism but also makes easy to understand GSD in space design. In particular, the study will reveal how the ideas of post-structural philosophy has effected the formation of its movement as well as its influence to become the theoretical nutrition for digital architecture. The result of the study will be used as the valuable reference to define 'Emotional Design'(So call 'Gam-Sung Design = GSD) and become the foundation for further investigation on GSD space design in the near future.

• Molecules and Cells
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• v.28 no.3
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• pp.139-148
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• 2009

Cell death has been traditionally classified in apoptosis and necrosis. Apoptosis, known as programmed cell death, is an active form of cell death mechanism that is tightly regulated by multiple cellular signaling pathways and requires ATP for its appropriate process. Apoptotic death plays essential roles for successful development and maintenance of normal cellular homeostasis in mammalian. In contrast to apoptosis, necrosis is classically considered as a passive cell death process that occurs rather by accident in disastrous conditions, is not required for energy and eventually induces inflammation. Regardless of different characteristics between apoptosis and necrosis, it has been well defined that both are responsible for a wide range of human diseases. Glycogen storage disease type I (GSD-I) is a kind of human genetic disorders and is caused by the deficiency of a microsomal protein, glucose-6-phosphatase-${\alpha}$ ($G6Pase-{\alpha}$) or glucose-6-phosphate transporter (G6PT) responsible for glucose homeostasis, leading to GSD-Ia or GSD-Ib, respectively. This review summarizes cell deaths in GSD-I and mostly focuses on current knowledge of the neutrophil apoptosis in GSD-Ib based upon ER stress and redox signaling.