• Asian-Australasian Journal of Animal Sciences
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• v.32 no.11
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• pp.1789-1800
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• 2019

Objective: Although alveolar macrophages play a key role in the respiratory immunity of livestock, studies on the mechanism of differentiation and survival of alveolar macrophages are lacking. Therefore, we undertook to investigate changes in the lipid metabolism and survival rate, using 3D4/31 macrophages and Dudleya brittonii which has been used as a traditional asthma treatment. Methods: 3D4/31 macrophages were used as the in vitro porcine alveolar macrophages model. The cells were activated by exposure to phorbol 12-myristate 13-acetate (PMA). Dudleya brittonii extraction was performed with distilled water. For evaluating the cell survival rate, we performed the water-soluble tetrazolium salt cell viability assay and growth curve analysis. To confirm cell death, cell cycle and intracellular reactive oxygen species (ROS) levels were measured using flow cytometric analysis by applying fluorescence dye dichlorofluorescein diacetate and propidium iodide. Furthermore, we also evaluated cellular lipid accumulation with oil red O staining, and fatty acid synthesis related genes expression levels using quantitative polymerase chain reaction (qPCR) with SYBR green dye. Glycolysis, fatty acid oxidation, and tricarboxylic acid (TCA) cycle related gene expression levels were measured using qPCR after exposure to Dudleya brittonii extract (DB) for 12 h. Results: The ROS production and cell death were induced by PMA treatment, and exposure to DB reduced the PMA induced downregulation of cell survival. The PMA and DB treatments upregulated the lipid accumulation, with corresponding increase in the acetyl-CoA carboxylase alpha, fatty acid synthase mRNA expressions. DB-PMA co-treatment reduced the glycolysis genes expression, but increased the expressions of fatty acid oxidation and TCA cycle genes. Conclusion: This study provides new insights and directions for further research relating to the immunity of porcine respiratory system, by employing a model based on alveolar macrophages and natural materials.

• Proceedings of the Korea Water Resources Association Conference
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• 2015.05a
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• pp.26-26
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• 2015

기후변화나 인위적인 요인 등에 의해 수문 자료에 비정상성(nonstationarity)이 나타나면서 정상성 가정 하에서 수행되는 빈도해석으로는 정확한 확률수문량 산정이 어려운 실정이다. 최근 이를 보완하기 위한 비정상성 빈도해석에 대한 연구가 진행되고 있고, 이와 더불어 비정상성 지역빈도 해석에 대한 관심도 높아지고 있다. 비정상성 지역빈도해석은 대개 홍수지수법(index flood method)을 기반으로 진행되고 있는데, 홍수지수와 성장곡선(growth curve)에 시간에 따른 변화를 고려하느냐의 여부에 따라 다양한 형태의 홍수지수모형이 적용되고 있다. 본 연구는 다양한 형태의 홍수지수모형의 성능을 평가하여 비정상성 자료에 적합한 형태를 선정하는 것을 목적으로 한다. 이를 위해 위치 매개변수가 시간에 따라 변화하는 비정상성 GEV 분포(GEV100)를 모분포로 하는 지점들로 지역들을 구성하고, Monte Carlo 모의를 통해 발생시킨 자료에 여러 형태의 홍수지수모형을 적용하여 각 모형의 성능을 평가하였다. 모의실험 결과 홍수 지수는 시간에 따른 변화가 없고, 성장곡선은 시간에 따라 변화하는 형태인 홍수지수모형이 다른 형태의 모형에 비해 대체로 더 정확한 확률수문량을 산정할 수 있는 것으로 나타났다. 또한 우리나라 기상청 관할 강우 관측 지점들 중 GEV100 분포가 적합한 것으로 선정된 지점들을 하나의 지역으로 구성하여 모의실험에서 적용한 것과 동일한, 여러 형태의 홍수지수모형을 적용한 결과 모의실험 결과와 일치하게 성장곡선에만 비정상성 고려된 홍수지수모형이 상대적으로 정확한 확률강우량을 산정하는 것으로 나타났다. 따라서 GEV100 모형 기반의 비정상성 지역빈도해석을 수행하기 위해서는 성장곡선만 시간에 따라 변화하는 홍수지수모형이 적합할 것으로 판단된다.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.5
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• pp.836-840
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• 2011

In order to isolate thermophilic compost-promoting bacteria with high activity of cellulase and xylanase, spent mushroom substrates with sawdust were collected from mushroom cultivation farm, Jinju, Gyeongnam in Korea. Among of the isolates, one strain, designated SJ21 was selected by agar diffusion method. The strain SJ21 was identified as members of the Bacillus lincheniformis by biochemical characteristics using Bacillus ID kit and VITEK 2 system. Comparative 16S rDNA gene sequence analysis showed that strain SJ21 formed a distinct phylogenetic tree within the genus Bacillus and was most closely related to Bacillus subtilis with 16S rDNA gene sequence similarity of 99%. On the basis of its physiological properties, biochemical characteristics and phylogenetic distinctiveness, strain SJ21 was classified within the genus Bacillus, for which the name Bacillus sp. SJ21 is proposed. The cellulase and xylanase activity of Bacillus sp. SJ21 was slightly increased according to bacterial population from exponential phase to stationary phase in growth curve for Bacillus sp. SJ21.

• Journal of the Korean Society of Safety
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• v.34 no.4
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• pp.1-5
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• 2019

In the field of combined cycle power generation, thermal barrier coating(TBC) protects the super-heat-resistant alloy, which forms the core component of the gas turbine, from high temperature exposure. As the turbine inlet temperature(TIT) increases, TBC is more important and durability performance is also important when considering maintenance cost and safety. Therefore, studies have been made on the fabrication method of TBC and super-heat-resistant alloy in order to improve the performance of the TBC. In recent years, due to excellent properties such as high temperature creep resistance and high temperature strength, turbine blade material have been replaced by a single crystal superalloy, however there is a lack of research on TBC applied to single crystal superalloy. In this study, to understand the isothermal degradation performance of the TBC applied to the single crystal superalloy, isothermal exposure test was conducted at various temperature to derive the delamination life. The growth curve of thermally grown oxide(TGO) layer was predicted to evaluate the isothermal degradation performance. Also, microstructural analysis was performed by scanning electron microscope(SEM) and energy dispersive X-ray spectroscopy (EDS) to determine the effect of mixed oxide formation on the delamination life.

• Journal of the FoodService Safety
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• v.1 no.1
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• pp.27-35
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• 2020

Salt is a common seasoning agent used in various processed foods, especially in kimchi and salted seafood (jeotgal). This study was conducted to investigate the efficacy of salt with antimicrobial substances (acetic acid, garlic extract, carvacrol, nisin, thymol, and their combination (acetic acid+nisin+thymol)) on improvement of antibacterial effects of salt against foodborne pathogens. Salt (10%) was prepared using six different types of 0.2% natural antimicrobial substances. The antibacterial effect of salt combined with natural antimicrobial substances was evaluated against foodborne pathogens using the broth micro-dilution method and growth curve plotted using absorbance measurements. For the five foodborne pathogens, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of salt without antimicrobial substances as control were in the range of 24~>50,000 ㎍/mL and >50,000 ㎍/mL, respectively. Salt with nisin, thymol, or garlic extract showed strong inhibitory effects and their MIC against L. monocytogenes were 49, 12,500, and 24 ㎍/mL, respectively. In particular, salt with nisin showed inhibitory activities against Gram-positive bacteria. However, all the antimicrobial substances were less effective against Gram-negative bacteria such as E. coli O157:H7 and S. Typhimurium than Gram-positive bacteria. These results could be used for the development of salt with natural antimicrobial substances especially targeted against L. monocytogenes. This would enable the lowering of saline concentration while improving the storability of food.

• Journal of Surface Science and Engineering
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• v.51 no.6
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• pp.393-399
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• 2018

Formation behavior of aluminum anodic oxide (AAO) films on Al6061 alloy was studied in view of thickness, morphology and defects in the anodic films in 20 vol.% sulfuric acid solution at a constant current density of $40mA/cm^2$, using voltage-time curve, observation of anodized specimen colors and surface and cross-sectional morphologies of anodic films with anodization time. With increasing anodizing time, voltage for film formation increased exponentially after about 12 min and its increasing rate decreased after 25 min, followed by a rapid decrease of the voltage after about 28 min. Surface color of anodized specimen became darker with increasing anodizing time up to about 20 min, while it appeared to be brighter with increasing anodizing time after 20 min. The darkened and brightened surfaces with anodizing time are attributed to an increase in thickness of porous anodic oxide film and a chemical damage of the films due to heat generated by increased resistance of the film, respectively. Cross-sectional observation of AAO films revealed the formation of defects of crack shape at the metal/oxide interface after 15 min which prevents the growth of AAO films. Width and length of the crack-like defect increased with anodizing time up to 25 min of anodizing, and finally the outer part of AAO films was partly dissolved or detached after 30 min of anodizing, resulting in non-uniform surface structures of the AAO films.

• International Journal of Advanced Culture Technology
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• v.6 no.4
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• pp.323-330
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• 2018

The growth of the older population is expected to further increase social problems associated with population aging, such as isolation, poverty, and depression. The emerging issues associated with the older population are also expected to provide further momentum on studies about the dwelling environment as factors that ensure the health of older people as well as improve their quality of life. Therefore, approaches for explaining the issues of the older age group should be diversified using a variety of factors and appropriate analytic tools. Studies on measuring depression have principally focused on assessing an objective self-report questionnaire, usually in a highly structured, textual form which may not reflect the cognitive impairment of older adults. The aim of this study was to define and measure dwelling depression among older adults in Korea. There are two specific hypotheses in this study as follows: (a) there will be statistically significant relationships with dwelling dissatisfaction and depression, and (b) dwelling depression tools containing text and images will be, respectively, assessment tools that have a good construct with content validity and reliability. In the first experiment, to define and measure dwelling depression, 301 people over 65 years old living in single and two-person households were surveyed using a text-based dwelling depression questionnaires from September 1-30, 2017. In the second experiment, to examine whether the projective image questionnaire could serve as a suitable replacement for the text-based questionnaires, the same participants were surveyed from January 22 to February 2, 2018. The results show that depression has a close correlation with dwelling dissatisfaction. In addition, the geriatric dwelling depression index (GDDI) based on the projective image was refined. Additionally, the projective image questionnaire has a close correlation with the text-based questionnaire. Finally, through ROC curve analysis, it was found that the projective image questionnaire can accurately predict a depression group. To this end, this preliminary study examined the validity of the projective image questionnaire in older adults to make this instrument feasible for older populations and to contribute to a profound understanding of geriatric depression due to the living environment. We hope they will provide a basis for further research on psychological diagnoses using projective images.

• Korean Journal of Materials Research
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• v.29 no.7
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• pp.456-462
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• 2019

ZnO thin-films are grown on a p-Si(111) substrate by RF sputtering. The effects of growth temperature and $O_2$ mixture ratio on the ZnO films are investigated by scanning electron microscopy (SEM), X-ray diffraction (XRD), and room-temperature photoluminescence (PL) measurements. All the grown ZnO thin films show a strong preferred orientation along the c-axis, with an intense ultraviolet emission centered at 377 nm. However, when $O_2$ is mixed with the sputtering gas, the half width at half maximum (FWHM) of the XRD peak increases and the deep-level defect-related emission PL band becomes pronounced. In addition, an n-ZnO/p-Si heterojunction diode is fabricated by photolithographic processes and characterized using its current-voltage (I-V) characteristic curve and photoresponsivity. The fabricated n-ZnO/p-Si heterojunction diode exhibits typical rectifying I-V characteristics, with turn-on voltage of about 1.1 V and ideality factor of 1.7. The ratio of current density at ${\pm}3V$ of the reverse and forward bias voltage is about $5.8{\times}10^3$, which demonstrates the switching performance of the fabricated diode. The photoresponse of the diode under illumination of chopped with 40 Hz white light source shows fast response time and recovery time of 0.5 msec and 0.4 msec, respectively.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.12
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• pp.507-514
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• 2018

This study utilized latent growth curve modeling to investigate the trajectories of adolescent life satisfaction changes in middle and high school students. The effects of self-esteem, career identity, school learning activity, gender, and household earnings on life satisfaction changes were examined. Data was obtained from the Korea Child Youth Panel Survey (KYCPS), a longitudinal study following students for 7 years. Year 3-6 data was utilized. Results found that the life satisfaction trajectory resulted as a quadratic model in which individual differences were significant. Second, school learning activity used as a time variant variable had a positive significant effect on life satisfaction each year. Third, gender and self-esteem as time invariant variables had significant effects on initial levels while self-esteem had effects on the slope and quadratic change. Further implications and research issues are discussed.

• Journal of Korea Foundry Society
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• v.38 no.6
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• pp.121-131
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• 2018

The effect of additives on the castability of the AC2BS aluminum alloy, which contains 35% recycled scrap, was investigated. For the wide utilization of the recycled scrap AC2BS aluminum alloy, the research results were compared to those with the AC2B virgin alloy, which is typical Al-Si-Cu alloy system. It was confirmed that the addition of Al-5%Ti-1%B increased the ${\alpha}$-Al nucleation temperature during solidification and decreased the grain size through cooling curve and microstructural observations of the recycled alloy. It was also found that an addition of Al-10%Sr decreased the eutectic Si growth temperature during the solidification process and modified the shape of the eutectic Si of the recycled alloy. The characteristics of fluidity, shrinkage and solidification crack strength were evaluated. For the AC2BS aluminum alloy containing 35% recycled scrap, both ${\alpha}$-Al grain refinement due to Ti-B and eutectic Si modifications due to Sr contributed to the improvement of the fluidity. The macro- shrinkage ratio increased with additions of both Al-10%Sr and Al-5%Ti-1%B and the micro-shrinkage ratio increased with the addition of Al-10%Sr but decreased with the addition of Al-5%Ti-1%B. The casting characteristics of TiB and Sr-treated AC2BS aluminum alloy containing 35% recycled scrap are similar to those of AC2B virgin alloy. The improvement of the solidification crack strength of the AC2BS aluminum alloy was possible by the reduction of the grain boundary the stress concentration through the enhancement by both ${\bullet}{\cdot}$-Al refinement and eutectic Si modification. More extensive use of the AC2BS aluminum alloy containing 35% recycled scrap can be expected in the future.