• 한방안이비인후피부과학회지
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• 제21권1호
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• pp.1-15
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• 2008

Objective : Skin aging is commonly observed in patients with diabetes mellitus, which can be accessed by the amount of skin collagen and matrix metalloproteinase-1 (MMP-1). In the present study, anti-skin-aging effects of Root Cortex of Paeonia Suffruticosa Andrews (PSA), which has been widely used to treat diabetes mellitus, are investigated. Methods : Streptozotocin (STZ) was intraperitoneally injected to rats to induce diabetes. Body weights, feed intake, organ weights, blood glucose, and other biochemical index are determined in both normal and diabetic rats. In order to study the effect of PSA on skin aging, the amount of skin collagen was measured in diabetic rats after PSA treatments. Also, MMP-1 synthesis in UVB-irradiated human skin fibroblasts was investigated. Results : 1. When PSA was administered to STZ-induced diabetic rats, feed intake was significantly increased and blood glucose and total cholesterol were decreased in a dose-dependent manner. However, there are no differences in individual organ weights, GOT, and GPT. 2. A decrease of skin collagen in diabetic rats was significantly suppressed when PSA was treated. 3. PSA also inhibited MMP-1 synthesis in UVB-irradiated normal human skin fibroblasts, similar to retinoid, a well-known effective anti-skin-aging substance. Conclusion: PSA suppressed a collagen decrease in diabetic rats and inhibited MMP-1 synthesis in UVB-irradiated human skin fibroblasts. Therefore, the treatment of PSA is very effective to slow down the skin aging process.

• 대한한의학회지
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• 제19권1호
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• pp.5-18
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• 1998

The purpose of this study was to determine the effects of aqua-acupuncture of Laennec N HO (Hominis placenta Cervi pontotrichum cornu Carthami semen). on trabecular area and physiological change in ovariectomized osteoporotic rats. This experiment was performed to .investigate an antiosteoporosis. effects of Laennec N HO(Hominis placenta Cervi pontotrichum cornu Carthmi semen) aqua-acupuncture on the osteoporosis induced by both ovariectomy in Rats. So rats were divided into Sham group and OVX group. OVX groups were divided into control group, Exp. I group, Exp II group and Exp III group). The Ratios of trabecular area seen in ephiphysis and diaphysis of tibia and lumbar vertebral body and physiological change were as follows. 1. In the proximal ephiphysis of tibia, Exp I II III groups were significantly increased compared to that in control group (p<0.05). 2. In the diaphysis of tibia, Exp III group were significantly increased compared to that in control group (p<0.05). 3. In the first lumbar vertebral body, Exp II group were significantly increased compared to that in control group. 4. In the cortical thickness index of tibia, Exp III III groups were significantly increased compared to that in control group. 5. Osteoca1cin was increased in the Exp II group than those of other Experiment groups and control group, and Phosphorus was decreased in the Exp I II group than that in control group, But a significant difference was not seen. GOT, GPT and Alkaline phosphatase were decreased in all Experiment groups than that in control group. But a significant difference was not seen. From the above results, it might be suggested that aqua-acupuncture Laennec N HO(Hominis placenta Cervi pontotrichum cornu Carthami semen) prevented the increase in bone turnover and the decrese in bone mass induced by OVX in rats.

• 동아시아식생활학회지
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• 제14권6호
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• pp.634-639
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• 2004

An eight-week-old male ICR mouse, which was induced with acute alcohol and sub-acute alcohol poisoning condition, was administered with bohee tea(Camelia sinensis L) extract. After oral administration of bohee tea and inducement of acute alcohol poisoning condition, the mouses blood alcohol concentration became as low as that of the normal control group. Its decrease rate was 87.26%, in comparison with that of the positive control group. Moreover, its blood GOT activity decreased with a rate of 93.1 % until it reached the normal level, as opposed to that of the positive control group. In addition, the GOT activity, despite rising after the alcohol intake, decreased(p<0.05) significantly after administration of each sample and reached the normal level. The bohee tea group experienced a significant decrease in the GOT activity, compared with the A1codex group and the Drink group. The GPT activity of the Alcodex group decreased by 11 % compared with that of the positive control group. The CTP activity of the bohee tea group decreased by 8.2%, while that of the Drink group decreased by 6.5%(P<0.05). However, there were no significant differences between the results in the control group and those of the test group. The bohee tea group's hepatic ADH activity increased by 22.7% compared with that of the positive control group. On the other hand, the hepatic ADH activity of the Drink group increased by 33.6% while that of the A1codex group increased by 20.4%. On the contrary, the bohee tea extract, the hepatic ALDH did not manifest any significant difference as compared with the normal control group. However, its decrease rate was about 16.67% as compared with that of the positive control group. The Drink group, meanwhile, obtained a decrease rate of about 21.59%.

• Journal of Acupuncture Research
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• 제18권6호
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• pp.188-205
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• 2001

Objective : The purpose of this studies was to determine the effect of Carthami semen, Bogol-Tang and Rhynchosia volubilis Lour. on changes of trabecular area and physiological metabolites in the ovariectomized osteoporotic Rats. Methods : In order to induce estrogen deficient osteoporosis, ovariectomy was done on rats. Then the Carthami semen, Bogol-Tang and Rhynchosia volubilis Lour. were orally administerd: Such indexes were measured as the changes of body weight, bone mineral density, trabecular area in tibia, and levels of osteocalcin, bone alkaline phosphatase, calcium, phosphorus, cholesterol, triglyceride, aspartate aminotransferase and alanine aminotransferase in serum. Results : 1. The change of bone mineral density in Bogol-Tang group and Rhynchosia volubilis lour. group. was significantly increased compare to control group. 2. The change of trabecular area % in epiphysis of tibia in Carthami semen group was significantly increased compare to control group. 3. The change of trabecular area % in diaphysis of tibia in Bogol-Tang group was significantly increased compare to control group. 4. The change of serum osteocalcin in Bogol-Tang group was significantly decreased compare to control group. 5. The change of serum bone alkaline phosphatase in Bogol-Tang group was significantly decreased compare to control group. 5. The change of serum bone alkaline phosphatase in Bogol-Tang group was significantly decreased compare to control group. 6. The change of1 serum calcium in Rhynchosia volubilis lour. group was significantly decreased compare to control group. 7. The change of phosphorus in Carthami semen group and Rhynchosia volubilis Lour. group was significantly decreased compare to control group. 8. The change of serum cholesterol and triglyceride of experimental groups were decreased in comparison with control group. 9. The change of serum AST(Aspartate aminotransferase : GOT) in Rhynchosia volubilis Lour. group was decreased compare to control group. 10. The change of serum ALT(Alanine aminotransferase : GPT) in Bogol-Tang group was decreased compare to control group.

• 대한지역사회영양학회지
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• 제7권2호
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• pp.219-231
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• 2002

The purpose of this study was to collect basic data on the prevention of and education about diabetes mellitus for the nutritional management of a diabetes mellitus risk group. The study which took place in Kangbukgu, Seoul, involved a diabetes mellitus risk group (DMR $\geq$ 110 mg/dL, 61), of males and females, aged 36 to 68 years, and a group of healthy people as a control group ( < 110 mg/dL, 183), using luting blood sugar (FBS) levels. The proportion of people in the abnormal range was higher in the DMR than that of control group for total cholesterol, high-density lipoproteins-cholesterol (HDL-C), total protein, glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT) and creatinine. Particularly with respect to serum protein the proportion in the DMR in abnormal range (p < 0.05) was significantly higher than that of the control group. The proportion in the DMR with a family history of disease was significantly higher than that of the control group (p < 0.01). Using body mass index (BMI), waist-hip ratio (WHR) and relative body weight (RBW), the obesity indices in the DMR was higher than that of the control group. Comparing the DMR and the control group with respect to dietary habits, it seems that the DMR had more undesirable dietary habits than the control group. When the intake of each nutrient for the DMR and the control group was compared to the Korean recommended dietary allowances (RDA), the proportion of excess intake and deficient intake in the DMR was higher than that of the control group. The DMR showed a greater undesirable dietary intake pattern as compared to that of the control group, based on the RDA. With respect to the dietary diversity score (DDS) and the mean adequacy ratio (MAR) for quality estimation of the overall flood intake, the DMR showed a feater undesirable pattern than the control group. According to the above results, the DMR tended to have more undesirable eating habits when compared to the control group. Therefore, to provide a more efficient nutritional education program for the DMR we must conduct lurker studies on eating habits, so as to provide systematic nutritional management based on theme differences between the DMR and the control group.

• 한국식품영양과학회지
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• 제29권1호
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• pp.168-173
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• 2000

The purpose of this study was to investigate the changes of microsomal mixed function oxidase (MFO) and xanthine oxidase activities (XOD) in the liver of rats exposed to microwave. Sprague-Dawley male rats weighing 200$\pm$10g body weight were randomly assigned to a normal and microwave exposed(MW) groups; microwave exposed groups were divided into two groups; microwave (MW) group and green tea(GT) gropu which were fed distilled water and green tea extracts during experimental periods, respectively. Rats were irradiated with microwave at the frequency of 2.45 GHz for 15min and rats wre sacrificed at the 4th day of the microwave irradiaton. The hemoglobin level of GT group was higher than that of the normal gropu and MW group, but the hematocrit value was not significantly different among all experimental gropus. The activity of serum GOT of MW group was significantly increased but that of GT group was similar to normal group. Activities of GPT were not significantly different among all experimental groups. Liver XOD activity was significantly increased in the microwave exposed groups but green tea normalized the XOD activity. The activity of hepatic microsomal cytochrome P450 was significantly increased in MW group compared to normal group and that of GT group was similar to that of the normal group. The activity of hepatic microsomal NADPH-cytochrome P450 reductase was also significantly increased in MW group compared to normal group, but that of GT group was similar to that fo the normal group. In conclusion, the activities of MFO and XOD were elevated by microwave irradiaton, but the activation of MFO system as well as the damage of the liver by microwave were reduced by green tea supplementation.

• 대한영양사협회학술지
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• 제12권3호
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• pp.289-298
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• 2006

We studied the effects of intake of the steamed bean powder(SB) and chungkukjang powder(CK) on blood parameters such as glucose, albumin, GOT, GPT, hematocrit, hemoglobin, total-/HDL-/LDL-cholesterol and triglyceride, and blood antioxidant nutrients such as retinol, tocopherols and carotenes in 26 college women. There were 2 groups, one group had 13 participants who consumed 30g SP and second group (13 participants) consumed 30g CK daily for 4 weeks. The study compared before with after SP/CK intake. The average age of the participants was 22.1 years, there were no significant differences between the two groups in terms of all parameters before the study. The energy intake showed no significant differences between before and after SP/CK intake. C(carbohydrate) propotion was reduced, and P(protein) and F(fat) ratio were increased after 4 week intake of SP/CK. Indices of nutritional quality(INQ) of protein(p<0.01), iron(p<0.05), zinc(p<0.05), vitamin C(p<0.05) were significantly increased after SP intake, and those of protein(p<0.05) and zinc also increased after CK intake. There were no significant differences in TG, total-/HDL-/LDL-cholesterol before and after SP/CK intake. The CK intake group showed the reduction only in retinol level in blood(p<0.05). Some students reported the improvement of the constipation and the regularity of the bowel elemination, and some students experienced the prolongation effects on one's monthly days after SP/CK intake. In this study, we could see the effect of improvement in some nutrients intake, but no effect in blood parameters by SP/CK intake for 4 weeks. That reason might be that the participants were healthy young women, or the trail period was not enough long to change.

• 대한영양사협회학술지
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• 제14권4호
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• pp.371-381
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• 2008

In order to elucidate the effects of a lacto-ovo vegetarian diet on serum lipid levels, 91 male collegians residing in the Seoul area were recruited. The vegetarians did not smoke tobacco nor drink alcohol. Their anthropometric measurements, dietary intakes, and blood lipid concentrations were compared to age-matched omnivorous controls. The subjects were divided into four groups. The four groups were: 1) NSNDV (n=25): Non Smoking Non Drinking Vegetarians, 2) NSNDO (n=10): Non Smoking Non Drinking Omnivores, 3) SDO (n=37): Smoking, Drinking Omnivores, 4) SDM (n=11): Smoking Drinking Meat-eaters. Total serum cholesterol, HDL-cholesterol and hematocrit were analyzed. The results were as follows: Weight (p<0.05) and BMI (p<0.01) were significantly higher in SDM than NSNDV. Dietary protein (p<0.01), iron (p<0.05) and dietary lipids (p<0.01) from animals in NSNDV were significantly lower than the results in the SDM. Interestingly enough, we noted no significant differences between the four groups in terms of average total dietary protein, dietary lipids and dietary iron, total serum cholesterol, or HDL-cholesterol. Dietary fiber intake was significantly higher in NSNDV than in SDM (p<0.05). Weight, BMI, and total cholesterol were significantly positively correlated with animal protein (p<0.05) and animal lipid (p<0.01) intake. These results showed that vegetarian collegians who haven't smoked tobacco nor drunk alcohol evidenced lower body weight, BMI, and dietary animal lipid intake, but higher dietary fiber intake, than the other three groups. Additionally, intake of animal protein (p<0.001) and intake of animal lipids (p<0.001) were significantly positively correlated with body weight, BMI, and total serum cholesterol in male collegians.

• 동아시아식생활학회지
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• 제22권2호
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• pp.231-238
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• 2012

$Dioscorea$ $batatas$ Decne is a type of yam that eaten raw and used as a traditional oriental medicine in Asian countries. In this study, we evaluated the functional effects of yam water extracts on body weight levels and serum lipid concentrations in mice fed high fat diet. Mice were divided into four groups: normal diet control (ND), high fat diet control (HFD), HFD+yam extract 100 mg/kg (HFD-Y100), and HFD+yam extract 200 mg/kg (HFD-Y200). Yam extract was administrated orally to mice fed a high fat diet for 5 weeks. Treatment with yam extract significantly reduced body weight levels and energy efficiency in a dose-dependent manner in HFD-fed mice. Yam extract also attenuated serum levels of triglyceride, total cholesterol, and LDL-cholesterol, as well as organ weights of liver and abdominal adipose tissue in mice fed a high fat diet. Moreover, blood levels of glucose, insulin, and leptin significantly decreased in a dose-dependent manner upon administration of yam extract. Blood activities of GPT, GOT, and LDH were lower in the yam extract-treated groups compared to the HFD group. These results indicate that yam water extract may reduce elevated body weight and serum lipid concentrations in mice fed a high fat diet, suggesting its usefulness as a functional food for reducing body fat and hyperlipidemia.

• 동의생리병리학회지
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• 제21권6호
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• pp.1462-1469
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• 2007

This study has been carried out to understand the effect of Gangsim-tang on the hyperglycemic mice induced with Streptozotocin(STZ). The 60 mg/kg of streptozotocin(STZ) was treated into mice twice by 24 hrs interval and then 120 mg/kg STZ was treated again 3 days after the earlier treated. Control group was administered mice with 0.9％ saline(2ml/kg), and experimental groups were administered Gangsim-tang extract(GA group, 10 ㎎/㎏/day; GB group, 30 mg/kg/day) after hyperglycemic induction daily for 6 weeks. The body weight of experimental groups was lower than control. The blood glucose concentration increased continuously, reaching to 298.9 mg/dl after 6 weeks, however, experimental groups of the GA and GB groups significantly(p<0.01) decreased in the 4, 5, and 6 weeks groups. Blood glucose tolerance test was not significant between control and experimental groups. We examined the blood transaminase activities to know the effect of herbal medicine on liver function. The glutamic-oxaloacetic transaminase(GOT) activity was lower in group GB than in control. The glutamic-pyruvic transaminse(GPT) activity was lower in group GA and GB than in control. The superoxide dismutase(SOD) and catalase activities were higher in the group GA compared to control. The results of immunohistochemical study, Langerhan's islet of pancreas was destructed by treatment of STZ in the control, and a few of insulin positive cells observed in the control and experimental group. These results suggest that administration of Gangsim-tang extract to the hyperglycemic mice induced with STZ not regeneration of ${\beta}-cells$ but control of the blood glucose level.