• Journal of the East Asian Society of Dietary Life
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• v.14 no.6
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• pp.634-639
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• 2004

An eight-week-old male ICR mouse, which was induced with acute alcohol and sub-acute alcohol poisoning condition, was administered with bohee tea(Camelia sinensis L) extract. After oral administration of bohee tea and inducement of acute alcohol poisoning condition, the mouses blood alcohol concentration became as low as that of the normal control group. Its decrease rate was 87.26%, in comparison with that of the positive control group. Moreover, its blood GOT activity decreased with a rate of 93.1 % until it reached the normal level, as opposed to that of the positive control group. In addition, the GOT activity, despite rising after the alcohol intake, decreased(p<0.05) significantly after administration of each sample and reached the normal level. The bohee tea group experienced a significant decrease in the GOT activity, compared with the A1codex group and the Drink group. The GPT activity of the Alcodex group decreased by 11 % compared with that of the positive control group. The CTP activity of the bohee tea group decreased by 8.2%, while that of the Drink group decreased by 6.5%(P<0.05). However, there were no significant differences between the results in the control group and those of the test group. The bohee tea group's hepatic ADH activity increased by 22.7% compared with that of the positive control group. On the other hand, the hepatic ADH activity of the Drink group increased by 33.6% while that of the A1codex group increased by 20.4%. On the contrary, the bohee tea extract, the hepatic ALDH did not manifest any significant difference as compared with the normal control group. However, its decrease rate was about 16.67% as compared with that of the positive control group. The Drink group, meanwhile, obtained a decrease rate of about 21.59%.

• Journal of the Korean Society of Food Science and Nutrition
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• v.28 no.3
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• pp.632-637
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• 1999

The purposes of this study is to investigate the effect of Rhus chinensis gall extract on liver function, plasma lipid composition and antioxidant system in the obese rats with high fat diet for seven weeks. Thirty two male rats of Sprague Dawely strain were divided into four groups. they are normal group (basal diet), control group(high fat diet), III group(high fat diet with Rhus chinensis gall extract 25mg/kg body weight per day) and IV group(high fat diet with Rhus chinensis gall extract 250mg/kg body weight per day). Rats in III group and IV group were administered with Rhus chinensis gall extract accordingly. Weight gains showed a tendency to decrease in rat with Rhus chinensis gall extract group but showed no differences among treatment groups. Food intake and Food efficiency ratio were not significantly different among treatment groups. Plasma total cholesterol showed a tendency to decrease in Rhus chinensis gall extract group. HDL cholesterol showed a tendency to decrease in 25mg/kg of Rhus chinensis gall extract group. However, in the 250mg/kg of Rhus chinensis gall extract group, these values showed no significant difference compared to the control group (p<0.05). GPT activities showed no significant difference among treatment groups. GOT activities showed a tendency to decrease in the groups of Rhus chinensis gall extract groups. Lipid peroxide level was significantly higher in control group than those of normal group. In Rhus chinensis gall extract groups, lipid peroxide level showed a tendency to decrease, but glutathione peroxidase activity was progressively increased. Results of our research in this paper show that Rhus chinensis gall extract might improve liver function, antioxidant system and plasma lipid composition in rats with high fat diet.

• Fisheries and Aquatic Sciences
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• v.22 no.1
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• pp.1.1-1.8
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• 2019

Five experimental diets were formulated to evaluate the effects of dietary enzymatically hydrolyzed tuna by-product on growth, non-specific immune responses, and hematology of juvenile rainbow trout (Oncorhynchus mykiss). A basal diet with 50% of fishmeal was used as control (CON) and four other diets replaced 12.5% ($TBB_{12.5}$), 25% ($TBB_{25}$), 37.5% ($TBB_{37.5}$), and 50% ($TBB_{50}$) of fish meal in the CON diet. Juvenile rainbow trout ($4.87{\pm}0.05g$) were randomly distributed into 15 tanks (50 L) and fed 3-4% of wet body weight two times a day. At the end of 7 weeks of feeding trial, weight gain, specific growth rate, feed efficiency, and protein efficiency ratio of fish fed CON diet were significantly higher than those of fish fed $TB_{50}$ diet (P < 0.05). But there were no significant differences among fish fed CON, $TBB_{12.5}$, $TBB_{25}$, and $TBB_{37.5}$ diets (P > 0.05). There were no significant differences in GPT levels among fish fed CON, $TBB_{12.5}$, $TBB_{25}$, and $TBB_{37.5}$ diets. Also, there were no significant differences in lysozyme, superoxide dismutase, glucose, and total protein levels in all experimental diet (P > 0.05). The broken-line analysis indicated that the minimum dietary level of enzymatically hydrolyzed tuna by-product to replace fishmeal could be 29.7% in rainbow trout. These results indicated that the optimum level of dietary enzymatically hydrolyzed tuna by-product could replace greater than 29.7% but less than 37.5% of fishmeal in juvenile rainbow trout diet.

• Journal of Ginseng Research
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• v.1 no.1
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• pp.59-78
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• 1976

In order to investigate the influences of Panax ginseng (white ginseng and red ginseng) on the anesthetic effect and toxic effect of alcohol, experimental studies .had been carried out with albino rabbits, mongolian dogs and mice. The anesthetic effect of alcohol was observed by measuring the induction time, .anesthetic time, recovery time and duration from the beginning of induction to , the recovery of anesthesia (total time), respectively. and toxic effect ($LD_{50}$) of alcohol was measured. In addition to these experiments, al cohol concentration in .blood, blood sugar level, serum transaminase (GOT and GPT) activities and serum alkaline phosphatase activity were measured. Also in order to study the clinical effect of alcohol in healthy students, code .substitution, response time and muscle coordination were tested. The results were obtained as follows. 1. In the rabbits and mongolian Jags, the induction time of anesthesia by the administration of alcohol was delayed by the pretreatment of ginseng but recovery time and total time of anestksia were markedly shortend. 2. The bleed alcohol concentration was decreased by the pretreatment of ginseng , but not affected in mongolian dogs. 3. The blood sugar level, serum transaminase (GOT and GPT) activities and alkaline phoshatase activity in rabbits and dogs induced by the administration of alcohol were affected by the Pretreatment of ginseng. Because those were included within normal ranges, the differnces have no remarkable significance. 4. Liver alcohol dehydrogenase activity of rabbit was increased by the treatment of ginseng, especially it was markedly increased by the treatment of red ginseng 5. The average lethal dose of alcohol to mice was increased by the pretreatment. of ginseng, especially it was markedly increased by the pretreatment of red .ginseng. 6. In the clinical experiments, the blood alcohol concentration induced by alcohol administration was not affected by the pretreatment of ginseng whereas the bleed sugar level was increased. Blood alcohol concentration and bleed sugar level were measured after three hours alcohol administration. 7. The response time of healthy students administered with alcohol was markedly shortened by the pretreatment of ginseng but the experiments on the code substitution and muscle coordination were not affected.

• Korean Journal of Rural Living Science
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• v.10 no.2
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• pp.71-77
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• 1999

This study examined the effect of the pine needle sap administration on serum lipid composition and liver antioxidant defense system in rats fed with high fat diet. Forty eight male Sprague-Dawley rats were divided into six groups. three control groups were fed with 5% lipid diet and three high fat groups were fed with 30% lipid diet. Each group was administered with the following pine needle sap respectively : C-0.0, 1.ml water, C-0.5, 0.ml pine needle sap and 0.ml water C-1.0, 1.ml pine needle sap : H-0.0, 1.ml water, H-0.5, 0.ml pine needle sap and 0.ml water H-1.0, 1.0ml pinus needle sap. After 4 weeks of experimental periods the level of serum obtained and serum lipid was measured respectively. The results were as follows 1. Significant low level was observed for food intake, weight gain, FER in the experimental group administered with the pine needle sap. 2. No difference of the concentration of serum triglyceride was observed out the experimental group with 5% fat diet, however out of the experimental group with 30% fat diet it was low in (H-1.0) group where 1.0ml of the pine needle sap was administrated. 3. In the experimental group with 5% fat diet the concentration of serum total cholesterol and LDL cholesterol was low, especially the hypocholesteromic effect in serum administered by the pine needle sap was significantly high in group C-1.0 where 1.0ml of pine needle sap was administered. In the high fat group provided with 30% fat when the pine needle sap was administered there was a tendency that concentration of total cholesterol and LDL cholesterol decreased however there was no difference significantly. 4. Serum GOT activity was relatively high in high fat diet group. However, when 1$m\ell$ of pine needle sap was administered the activity was lower than that of the control group in H-1.0 group and the activity level was similar with that of the high fat diet group in c-1.0 group. There was no difference of serum GPT activitives followed by the administered of pine needle sap among the experimental group. 5. Liver TBARS levels were high in general in high fat diet group, however it showed no difference when the pine needle sap with different concentration level was administered. The results of this study indicate that the pine needle sap administration was effective in decreasing the food intake and weight gain of the experimental animals in the high fat diet and also effective in decreasing the levels of serum triglyceride, total cholesterol and LDL cholesterol and GOT activities. However it was not effective to change the level of TBARS and GSH -Px activities of liver. Thus, it was found that the pine needle sap administration was effective for the improvement of serum lipid composition condition of the experimental animals in the high fat diet group but it was not effective in the antioxidant defense system of liver.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.27 no.1
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• pp.59-69
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• 2017

Objectives: The objective of this study is to evaluate the exposure of VOCs and effects of the chemicals on the nail technicians whose works in a nail shop. Methods: For four month from May to August in 2016, we measured twenty-two kinds of VOCs in ten nail shops and carried out health examinations on thirty-four workers in there. Results: The TVOC concentration in indoor air of nail shops is $0.487mg/m^3$ at a minimum and $33.236mg/m^3$ at a maximum where it consists of 70.5% of Ketones, 25.4% of Alcohols, 2.6% of Esters, 0.8% of Aldehydes and 0.7% of Aromatics. The VOCs concentration during nail art works shows an increase in average ratio 1.8 compared to the concentration of indoor air quality and also the concentration of Isopropanol rose with 3.2 of the highest ratio. The results of Spearman correlation between TVOC concentration in indoor air and environmental factor was like that has significance level of correlation(${\rho}$<0.05, r=0.682) in case of number of customers per day, but the other factors were not meaningful in correlation. Correlation between VOCs and medical check-up items was like that has positive significance level(${\rho}$<0.01, r=0.638) between isopropanol and GPT, but the others have not meaningful. The exposure level of VOCs was not exceed the criteria exposure level 1 of working environment measuring method which announced by labor ministry in all ten nail shop indoor air quality. Conclusions: In this study although it was not significant correlation between harmful substances and medical check-up items in the nail shop indoor air quality, it is necessary to do more ventilation and to install exhaust facilities because of existing high VOCs concentration in the nail shop indoor air.

• Journal of the Korean Society of Food Science and Nutrition
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• v.34 no.2
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• pp.181-189
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• 2005

The purpose of this study was to investigate the relationship between obesity indices, nutritional knowledge, nutritional intake status, and some blood parameters in obese middle-school students. Anthropometric measurement, 24-hour recall for dietary intake, blood analysis and Questionnaire response including nutritional knowledge, nutritional attitude, and self-satisfaction were conducted in 42 obese middle-school students (28 male and 14 female) participated in the nutritional camp program carried by Bucheon district public health center in Kyunggi-do. The mean age of subjects was 13.9 years. The average body weight, BMI, obesity index, WHR and percent body fat were 75.5 kg, 29.1 kg/$m^2$, 30.1, 0.89 and 33.3% in male subjects, and 67.8 kg, 27.5 kg/$m^2$, 25.3, 0.81 and 34.6% in female subjects. The average nutritional attitude, the self-satisfaction and the nutritional knowledge scores were no significant difference between male and female subjects. Average intake of energy were 85.5% of RDA in males and 98.1% of RDA in females. The percentage of energy from fat was higher in both male (29%) and female (26%) subjects than recommended level. Calcium, iron, zinc, vitamin A, vitamin B$_2$, vitamin C, niacin and folate intakes were below the RDA for them. In the results of correlation analysis, body weight, BMI and obesity index were positively correlated with systolic blood pressure and cholesterol intake. There was a negative correlation between percent body fat and nutritional knowledge. Body weight was negatively related to HDL-cholesterol, whereas it has positive relation with RBC, hemoglobin and hematocrit. BMI and obesity index were negatively related to HDL-cholesterol, but positively related to RBC, respectively. WHR showed positive relationship with serum GPT, glucose and MCV.

• Journal of Life Science
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• v.20 no.5
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• pp.782-788
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• 2010

The protective effects of a powder mixed with solid-cultured and liquid-cultured Lentinus edodes mycelia (2 : 1, w/w) (designate LED) with different doses of carbon tetrachloride ($CCl_4$) on induced hepatotoxicity in male Sprague-Dawley (SD) rats was investigated. The rats were divided into seven groups (6 rats/group) and the following substances were administered orally to each group: Vehicle (0.2 ml distilled water), Control (0.2 ml distilled water), LED (LED 100, 200, 300 and 400 mg/kg BW in 0.2 ml distilled water), and Silymarin (200 mg/Kg BW in 0.2 ml distilled water). After two weeks of daily administration, all groups except for the Vehiclegroup were subjected to abdominal injection with $CCl_4$ ($CCl_4$ : corn oil, 1 : 1 v/v; 0.5 ml/kg BW). One day later, blood and liver samples were collected to analyze biomarkers. All LED treatments elevated hepatic superoxide dismutase (SOD), catalase and glutathione peroxidase (GSH peroxidase) activities, and reduced thiobarbituric reactive substances (TBARS), tumor necrosis factor-$\alpha$ (TNF-$\alpha$), interleukin-$1{\beta}$ (IL-$1{\beta}$) and interleukin-6 (IL-6), resulting in the reduction of glutamate-oxalate transaminase (GOT), glutamate-pyruvate transaminase (GPT) and lactic acid dehydrogenase (LDH) activities in plasma. These results indicate that LED effectively protected SD rat hepatotoxicity induced by $CCl_4$ through its antioxidative activity and reduction of some cytokines. The highest efficacy was found in LED 200 mg/kg BW, showing potential as a useful material for protection from hepatotoxicity in humans.

• Korean Journal of Food Science and Technology
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• v.42 no.2
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• pp.204-209
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• 2010

This study was conducted to investigate the anti-diabetic activity of Kocat-D1, which is widely used in traditional medicine to treat diabetes in Shandong, China. Sprague Dawley rats (8 weeks of age) were separated into 4 groups: a normal control, streptozotocin (STZ)-induced diabetic rat group (DM control), Kocat-D1-1 (diabetic rat treated with 0.25 g/kg/day hot water extract), and Kocat-D1-2 (diabetic rat treated with 1 g/kg/day hot water extract). After eight weeks of treatment, the fasting blood glucose levels of the Kocat-D1-1 ($334.3{\pm}32.9\;mg/dL$) and Kocat-D1-2 group ($259.5{\pm}35.0\;mg/dL$) were significantly lower when compared to the DM control group ($451{\pm}42.6\;mg/dL$). Furthermore, the levels of glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminase (GPT), albumin and high-density lipoprotein (HDL) cholesterol in the serum of the Kocat-D1-2 group were significantly normalized when compared to the DM control group. However, significant differences were not observed between the Kocat-D1-1 group and the DM control group. Histochemical staining of the liver of the Kocat-D1-2 group revealed no fat accumulation. The insulin level was significantly upregulated in the Kocat-D1-2 group ($0.13{\pm}0.02\;ng/mL$) when compared to the DM control group ($0.05{\pm}0.04\;ng/mL$). The relative volume of $\beta$-cells in the pancreas of the Kocat-D1-2 group ($49.4{\pm}4.2%$) also increased significantly when compared to the DM control group ($12.9{\pm}7.9%$). These results suggest that Kocat-D1 exerts an anti-hyperglycemic effect through the enhancement of insulin secretion.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.4 no.2
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• pp.224-239
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• 1994

In this study we examined several subjective symptoms in an exposed group and an unexposed group to perchloroethylene. The exposed group consists to 30 workers employed in 23 dry-cleaning establishments located in Chung Cheung Do, whereas the unexposed group consists of 42 officers employed in a certain university. This study was conducted from October, 1993 to March, 1994. Also we investigated personal exposure levels and area concentration of PCE, and performed hematological and biochemical examination in blood and urine samples from the exposed group. The results are : 1. The exposed group highly complains several subjective symptoms, particularly in neuropsychiatry, eye, respiratory system and digestive system, compared with the unexposed group. But complaint rates in the exposed group are not related with PCE exposure levels. 2. PCE exposure levels for all dry-cleaning establishment varied widely, from 0.18 to 37.58ppm. The mean exposure level for centers of chain was 16.85ppm, and for local laundries was 8.83ppm, while for self-service establishment it was 3.07ppm. Eighty three percent of the workers were exposed less than the half-level(25.0ppm) of the Korea 50.0ppm standard for eight-hour PCE exposure. Seventeen percent of them exceeded the half-level of the Korea TLV. 3. Statistical correlation exists between the work load(number of operation cycles of equipment and the mass of processed textiles) and the personal exposure levels to be observed. 4. In hematological examination the values of WBC, RBC, Hgb, Hct, MCV, MCH, MCHC and PLT in the exposed group were within normal ranges. In biochemical examination(GOT, GPT, ALP, U-A and LDH) most of the items were within normal ranges. From this surveys conducted on 23 dry-cleaning establishments, the complaint rates of the exposed group to PCE in several subjective symptoms were higher than those of the unexposed group, and PCE exposure levels in all dry-cleaning establishment were below the Koera standard 50.0ppm. In hematological and biochemical examination the significant abnormalities by occupational exposure to PCE were not observed. However, the workers employed in dry-cleaning establishments are always faced with risk, becauses they are also exposed to several organic solvents used to remove stains. Therefore, various measures to improve occupational environment in dry-cleaning establishment should be considered.