• Environmental Analysis Health and Toxicology
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• 제13권3_4호
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• pp.117-123
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• 1998

Paraquat is a useful nonselective herbicide widely used throughout the world. However, accidental or intentional ingestion of the paraquat cause fetal pulmonary injuring. But there is not suitable antidote of paraquat intoxication and therapeutic agents now be used are not effective. So, in this study we intended to evaluate the inhibitory effects of DDB(dimethyl-4,4'dimethoxy-5,6,5',6'-dimethylene dioxyphenyl-2,2'-dicarboxylate) on paraquat toxicity. DDB (100mg/kg) was administered orally to SD rats lhr after paraquat(50mg/kg) injection. After 24 hours, the biochemical parameters of blood and tissues were examined. In paraquat treated groups sGPT, sGOT, BUN, creatinine, MDA and alkaline phosphatase levels in blood and MDA, glucose-6-phosphatase activity in tissues were elevated by 2 to 5 times of normal values. However in schizandrin treated groups, sGPT, sGOT, MDA and alkaline phosphatase activity in blood and MDA and glucose-6-phosphatase activity were significantly decreased to notmal levels but not in biochemical parameters of nephrotoxicity, BUN and creatinine levels. Therefore, we concluded that schizandrin can be used as an antidote of pulmono, hepatotoxicity of paraquat.

• Environmental Analysis Health and Toxicology
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• 제13권3_4호
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• pp.125-131
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• 1998

Cisplatin is one of the most effective antitumor agents currently available for cancer chemotherapy. However its clinical use has been limited by its severe side effects, especially nephrotoxicity. To evaluate the effect of schizandrin, one of radical scavengers and constituents of Schizandra chimensis, cisplatin and schizandrin were given intraperitoneally. Protective effect of schizandrin against nephrotoxicity of cisplatin was observed when schizandrin was administerd to rats 1,24 hr after cisplatin injection. Hepatotoxicity induced by combination treatment of cisplatin and schizandrin was evaluated by measuring sGPT and sGOT. Combination treatment did not affect the levels of sGPT and sGOT. The present result indicate that schizandrin when it is given after cisplatin, may provide protection against cisplatin nephrotoxicity without hepatotoxicity.

• 한국환경보건학회지
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• 제33권3호
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• pp.202-206
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• 2007

Petasites japonicus (Compositae) is a perennial herb which has been used as treatment of antitussive, expectorant, sedatives, paralysis, diurectics in folk remedies. The pharmacological studies of this natural drug have not yet estabilished. So, we examined anti-lipid peroxidative effects and liver protective effect on $CCl_4$ induced lipid peroxidation and hepatotoxicity in rats. In vivo liver lipid, MeOH Ex. revealed significant increase of anti-lipid peroxidative effects according to concentration dependently. In chemical parameters obtained from serum analysis, MeOH Ex. as a increase of medicine concentration (0.25 g/kg, 0.5 g/kg, 1.0 g/kg), GOT, GPT, AlP decreased. In 0.5 g/kg GPT administered group, there was relative in GOT, AlP. Cholesterol decreased in 1.0 g/kg and 1.0 g/kg administered group, BUN decreased relatively in 1.0 g/kg administered group.

• 한방안이비인후피부과학회지
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• 제20권1호통권32호
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• pp.51-65
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• 2007

Objective : The present study was carried out to investigate the preventive effect of Sophorae Flos on streptozotocin - induced Diabetes mellitus. Method : Sophorae Flos was given to rats with oral administration. The experimental animals were divided into 3groups : normal group of rats, control group of Streptozotocin-induced diabetic rats, sample group with Sophorae Flos . The effect of Sophorae Flos on Streptozotocin-induced diabetes was observed by measuring the survival rate of rats, weight of rats, FER, blood glucose, each organ weight of rat, total cholesterol, HDL, GOT, GPT & creatinine. Result : Streptozotocin caused hyperglycemia and hypoinsulinemia by a selectively destroying pancreatic ${\beta}-cell$. Sophorae Flos treatment don't protected them from hyperglycemia and hypoinsulinemia. Organ weight liver, kidney, heart & spleen shows no significant changes. Sophorae Flos significantly don't recoverd the increase of several biochemical parameters such as blood glucose, total cholesterol, HDL, GOT, GPT, antioxidant & creatinine is vice versa. Conclusion : Sophorae Flos extract group did not show significant decrease than Streptozotocin control group.

• 약학회지
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• 제39권1호
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• pp.14-23
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• 1995

The current study was undertaken to select the crude drug showing life prolonging effect on the starved rats(Sprague-Dawley) and elucidate the reaction mechanism there of. As distilled water extracts prepared from twenty kinds of natural drugs were administered orally to female starved rats, Bupleuri Radix demonstrated the most prominent effect with 34.6% increment in the survival time. To investigate underlying mechanism of life prolonging effect of Bupleuri Radix, the concentrations of adrenocorticosteroids(corticsterone, testosterone and aldosterone) and enzyme activities of superoxide dismutase(SOD), glutamate oxaloacetate transaminase(GOT), glutamate pyrurate transaminase(GPT) were assayed in the serums of rats starved for, 2, 4 and 6 days respectively. The results manifested much elevated values of corticosterone, aldosterone(2 days) and rather decreasing tendency afterwards, specially in the late periods of starvation(6 days). With respect to such a considerable changes according to starvation periods, Bupleur Radix restored these values much near to normal suggesting that Buplerum Radix may play a life prolonging action during starvation probably through reinforcing the homeostatic properties of corticosteroids and some enzyme activities.

• 한국미생물·생명공학회지
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• 제28권4호
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• pp.209-213
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• 2000

The cultured mycelia of Ganoderma lucidum were extracted and the extract was separated into six fractions by organic solvent fractionation. The antihepatotoxic activity of all the fractions was evaluated by measuring activities of glutamic pyruvic transaminase (GPT) and glutamic oxaloacetic transaminase (GOT). Among the fractions tested, the high-polarity fractions such as aqueous and n-butanol fractions significantly reduced activities of GPT and GOT in CCl4- and galactosamine-intoxicated rat primary hepatocytes. When intracellular synthetic activities were measured by pulsing the rate primary cultured hepatocytes with [3H]-uridine and [3H]-leucine, activities of DNA, RNA and protein. When direct toxicities of the fractions were measured against human hepatoma(SK-Hep-1), the non-polarity fractions such as n-butanol and ethyl acetate fractions showed potent direct cytotoxicities even at the concentration of 1 $\mu\textrm{g}$/ml. These data showed that Ganoderma lucidum has hepatoprotective and hepatotoxic recovery principles in its mycelia.

• Environmental Analysis Health and Toxicology
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• 제17권3호
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• pp.261-264
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• 2002

The heartwood of Caeralpinia sappan L. (Leguminosae) has been used to activate blood flow, remove blood stasis, reduce swelling and relieve pain in Korean folk medicine. In this study. the antioxidant effects of this crude drug and its hepatoprotective activity on CCl$_4$-induced liver injury in rats were evaluated. The levels of antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT) reduced by CCl$_4$treatment, were recovered by this crude drug. It is suggested that Caesalpinia sappan L. have antioxidant effect. The increased levels of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) by CCl$_4$were also recovered by treatment with this crude drug. These results can be attributed to the agent's antioxidant and membrane - stabilizing actions.

• 한국식품영양학회지
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• 제7권2호
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• pp.108-113
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• 1994

This study was carried out to evaluate the effect of Cnidi rhizoma (CR) water extract on fat accumulation In fatted rats induced by the oral high fat administration for six weeks. To accomplish this evaluation, the serum and liver tissue have been examined for enzyme activity, cortisol and insulin level. The change of liver or tissue have been observed by the light microscope. GOT GPT and LDH activities were lower than the control group. Insulin and cortisol were higher than the control group, due to the fat accumulation. The liver of the control group observed by the tight microscope appeared to the fatty liver, but CR group showed some improvement of the fatty liver Based on the above results, it was shown that it is possible to improve fat accumulation induced by high fat dietary through using the oral administration of Cnidi rhizoma water extract.

• 한국식품영양과학회지
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• 제24권1호
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• pp.127-132
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• 1995

The protective effect of jujube methanol extract on benzo(a)pyrene(B(a)P)-induced liver injury was studied in rats in vitro and in vivo. Jujube methanol extract significantly recovered the enzyme activities(GOT, GPT, LDH and ALP) and lipid contents(total cholesterol, triglyceride and HDL-cholesterol) changed by B(a)P to normal levels in vivo. in viro experiment jujube methanol extract didn't stimulate hepatocyte proliferation but significantly recovered the enzyme activities(GOT, GPT and LDH) in comparison to group Ⅱ administered B(a)P only. It was suggested that jujube methanol extract have a protective effect on liver injury by B(a)P.

• 한국식품영양과학회지
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• 제25권1호
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• pp.34-38
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• 1996