• Title/Summary/Keyword: GM 작물

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Analysis of Housekeeping Genes in Mice Feeding on GM and non-GM Potatoes (해충저항성 GM감자와 non-GM감자의 Housekeeping gene 발현 분석)

  • Kweon, Mi-Ae;Heo, Jin-Chul;Cho, Hyun-Seok;Lee, Sang-Han
    • Food Science and Preservation
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    • v.15 no.4
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    • pp.562-567
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    • 2008

  • To develop human risk assessment protocols, we explored housekeeping gene and cytokine expression in mouse spleen cells using Rt-PCR. We normalized housekeeping gene expression by RT-PCR; gene expression was highly uniform in potato leafs and mouse spleen cells. We measured the expression of frequently used housekeeping genes, such as those encoding APRT, $\beta$-tubulin, Actin, Hsp 20.2, Cyclophilin, 18S RNA, Efla, Tbp, GAPDH, $\beta$-actin, Tuba2, Hprt, Cyclophlin A, Tfrc, and RPL13A in mice fed GM or non-GM potatoes. Housekeeping gene expression did not show any significant differences between GM and non-GM potato-fed mice. The murine model of potato-fed mice did not express IL-4 and IL-13 at a significant levels.

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Development of genetically modified crops based on considerations of risk assessment and management (위해성평가와 관리를 고려한 유전자변형작물 개발의 필요성)

  • Kim, Chang-Gi;Jeong, Soon-Chun;Yoon, Won-Kee;Park, Kee-Woong;Choi, Kyung-Hwa;Kim, Hwan-Mook
    • Journal of Plant Biotechnology
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    • v.36 no.4
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    • pp.360-365
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    • 2009

  • Over the last five years, we have conducted research on risk assessment of domestically developed genetically modified (GM) crops and found a number of factors which could delay risk assessment process. In this review, we described such cases and discussed the problem of transgene cassette integration, the lack of information on vectors, the poor quality control in seed production and absence of bioinformatic analysis on amino acid sequence homology before GM crop development. To solve these problems, we have suggested the introduction of the screening system of elite event before risk assessment process and quality control strategies for GM seed production. In addition, we suggested that the developers of GM crops should understand the importance of risk assessment and management for the commercialization of those crops and consider the biological and ecological characteristics of host plants. Consistent communications may need to be established between GM crop developers, risk assessors and risk managers at the initial stages of GM crop development to reduce trial-and-errors.

  • https://doi.org/10.5010/JPB.2009.36.4.360 인용 PDF KSCI

Assessment of gene flow from insect-resistant genetically modified rice (Agb0101) to non-GM rice (해충저항성 유전자변형 벼(Agb0101) 유전자 이동성 평가)

  • Oh, Sung-Dug;Yun, Doh-Won;Sohn, Soo-In;Park, Soon Ki;Chang, Ancheol
    • Korean Journal of Breeding Science
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    • v.49 no.3
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    • pp.180-189
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    • 2017

  • Genetically modified (GM) crops have been developed worldwide through the recombinant DNA technology and commercialized by global agricultural companies. Until now, GM crops have not been cultivated commercially in Korea. Commercialization of GM crops requires a compulsory assessment of environmental risk associated with the release of GM crops. This study was conducted to evaluate the frequency of pollen mediated gene flow from Bt transgenic rice (Agb0101) to japonica non-GM rice (Nakdongbyeo), indica non-GM rice (IR36), and weedy rice (R55). A total of 729,917, 596,318 and 230,635 seeds were collected from Nakdongbyeo, IR36, and R55, respectively, which were planted around Agb0101. Selection of the hybrids was determined by repeated spraying of herbicide and Cry1Ac1 immunostrip assay. Finally, the hybrids were confirmed by PCR analysis using specific primer. The hybrids were found in all non-GM rice and out-crossing ranged from 0.0005% at IR36 to 0.0027% at Nakdongbyeo. All of hybrids were located within 1.2 m distance from the Agb0101 rice plot. The meteorological elements including rainfall and temperature during rice flowering time were found to be important factors to determine rice out-crossing rate. Consideration should be taken for many factors like the meteorological elements of field and physiological condition of crop to set up the safety management guideline to prevention of GM crops gene flow.

  • https://doi.org/10.9787/KJBS.2017.49.3.180 인용