• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2002.11a
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• pp.87-87
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• 2002

초음파기기를 이용한 난포란의 채란 기술은 유전적으로 우수한 수정란의 다량확보가 가능함으로써 가축개량에 널리 응용할 수 있다. 그러나 초음파유도 난포란의 채란은 무엇보다도 회수율과 회수한 난포란의 질적 등급의 개선이 이루어져야 할 것이다. 본 연구는 등지방층두께, 일당증체량, 근내지방도 및 배최장근 단면적에 연관된 DNA marker가 검정된 한우로부터 FSH(FOLLTROPIN-V, Vetrepharm, Canada)를 투여하여 FSH 투여전.후의 포의 발달율과 등급별 회수율을 조사하였다. 공시동물의 발정주기에 관계없이 매주 무처리와 채란 12시간 전에 FSH를 각각 l00mg 과 200mg을 근육 주사하여 난포란의 채취를 유토하였다. 난소내 난포란의 채란은 SONOACE-600형 (Medison CO., 한국) 초음파기기를 사용하여 모니터 상에 검게 나타나는 난포를 17-gauge needle (Cook, Australia) 로써 70mmHg로 유지된 regulated vacuum pump를 이용하여 모두 채란하였으며, 소난포는 손가락 촉지를 이용하여 채란하였다. 난포의 크기는(large : $\geq$6mm, medium : 2-6mm, small $\leq$2mm) 무처리구에서 small(56.6%), medium(33.0%), large follicles(10.4%) 순으로 나타났으며, 전체 난포수는 106(8.2$\pm$3.6개) 였다. FSH 200mg 투여구에서는 medium(47.5%), small(32.5%), large follicles(20.0%) 순으로 나타났으며, 전체난포수는 40(10.0$\pm$2.2개) 였다. FSH l00mg 투여구에서는 medium(45.6%), small(43.5%), large follicles (10.9%) 순으로 나타났으며, 전체 난포는 92(7.7$\pm$2.5개) 였다. FSH 투여전 초음파 image 상으로 관찰한 난포수와 FSH 투여 후 초음파기기와 손가락 촉지를 이용하여 난포의 발달 및 난포란의 채란율은 200mg 투여 구에서는 투여 전에 4.3$\pm$0.6개였으나 투여 후에는 9.3$\pm$2.1개로 증가하였으며, 채란율은 75.0% 였다. l00mg 투여구에서도 투여 전후에 각각 5.3$\pm$1.2개 및 8.2$\pm$2.3개였으며, 채란율은 81.6%였다. 난포란의 채란율은 무처리구에서 88.7%(7.2$\pm$3.9개)로 나타났으며, 회수한 난포란의 등급은 각각 0.0%(GI), 5.3%(GII), 28.T%(GIII), 66.0%(GIV)로 나타났다. FSH 200mg 투여구의 채란율은 87.5%(8.8$\pm$2.6개)로 나타났으며, 등급별회수율은 GI(2.8%), GII(8.6%), GIII(34.3%), GIV(54.3%)으로 나타났다. 뿐만 아니라 FSH 100mg 투여구에서도 회수율은 89.1%(6.8$\pm$5.2개)였으며, 등급별 회수율은 각각 GI(8.5%), GII(13.4%), GIII(43.9%), GIV(34.2%)로 나타났다.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.26 no.11A
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• pp.1908-1924
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• 2001

Interoperability and inter-working in the various network and media environment with different technology background is very important to enlarge the opportunity of service access and to increase the competitive power of service. The ITU-T and advanced counties are planning ahead for provision of GII enabling user to access advanced global communication services supporting multimedia communication applications, embracing all modes of information. In this paper, we especially forced the heterogeneity of end user applications for multimedia networking. The heterogeneity has several technical aspects, like different medium access methods, heterogeneous coding algorithms for audio-visual data and so on. Among these elements, we have been itemized bit rate shaping algorithm on the compressed moving video. Previous manipulations of video has been done on the uncompressed signal domain. That is, compressed video should be converted to linear PCM signal. To do such a procedures, we should decode, manipulate and then encode the video to compressed signal once again. The traditional approach for processing the video signa1 has several critical weak points, requiring complexity to implement, degradation of image quality and large processing delay. The bit rate shaping algorithm proposed in this paper process the manipulation of moving video on the completely compressed domain to cope with above deficit. With this algorithms. we could realized efficient video bit rate shaping and the result of software simulation shows that this method has significant advantage than that of pixel oriented algorithms.

• Journal of Microbiology and Biotechnology
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• v.30 no.9
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• pp.1412-1419
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• 2020

Human noroviruses (HuNoVs) are a leading cause of gastroenteritis outbreaks worldwide. However, the paucity of appropriate cell culture models for HuNoV replication has prevented developing effective anti-HuNoV therapies. In this study, first, the replication of the virus at various temperatures in different cells was compared, which showed that lowering the culture temperature from 37℃ significantly increased virus replication in Madin-Darby canine kidney (MDCK) cells. Second, the expression levels of autophagy-, immune-, and apoptosis-related genes at 30℃ and 37℃ were compared to explore factors affecting HuNoV replication. HuNoV cultured at 37℃ showed significantly increased autophagy-related genes (ATG5 and ATG7) and immune-related genes (IFNA, IFNB, ISG15, and NFKB) compared to mock. However, the virus cultured at 30℃ showed significantly decreased expression of autophagy-related genes (ATG5 and ATG7), but not significantly different major immune-related genes (IFNA, ISG15, and NFKB) compared to mock. Importantly, expression of the transcription factor FOXO1, which controls autophagy- and immune-related gene expression, was significantly lower at 30℃. Moreover, FOXO1 inhibition in temperature-optimized MDCK cells enhanced HuNoV replication, highlighting FOXO1 inhibition as an approach for successful virus replication. In the temperature-optimized cells, various HuNoV genotypes were successfully replicated, with GI.8 showing the highest replication levels followed by GII.1, GII.3, and GII.4. Furthermore, ultrastructural analysis of the infected cells revealed functional HuNoV replication at low temperature, with increased cellular apoptosis and decreased autophagic vacuoles. In conclusion, temperature-optimized MDCK cells can be used as a convenient culture model for HuNoV replication by inhibiting FOXO1 and providing adaptability to different genotypes.

• Journal of Microbiology and Biotechnology
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• v.28 no.12
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• pp.2133-2140
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• 2018

Norovirus is the most common cause of acute gastroenteritis. Its pathogenesis is poorly understood owing to the difficulty of establishing viral infection in animal models. Here, post-weaning gnotobiotic pigs were infected with human norovirus genogroup II genotype 4 (HuNoV GII.4) to investigate the pathogenesis and replication of the virus. Three groups of four pigs were infected with $1{\times}10^5$, $1{\times}10^6$, or $1{\times}10^7$ genomic equivalent (GE) copies of HuNoV GII.4. Four pigs were used as negative controls. Blood and rectal swab samples were collected after viral infection, and gross legions were examined after necropsy. Diarrhea was induced in 25% and 75% of pigs infected with $1{\times}10^6$ and $1{\times}10^7$ GE copies, respectively. Viral shedding was detected in 50%, 75%, and 50% of pigs infected with $1{\times}10^5$, $1{\times}10^6$, and $1{\times}10^7$ GE copies, respectively. Viremia was detected in 25% of pigs infected with either $1{\times}10^6$ or $1{\times}10^7$ GE copies. When gross lesions of gastroenteritis were investigated, the ileum walls of the infected pigs were thinner than those of the controls. Villi atrophy and inflammatory cell infiltration were identified in the ileum of each infected pig. Viral capsid was identified in the jejunum, ileum, colon, spleen, and mesenteric lymph node. Virus replication was newly verified in the spleen and mesenteric lymph nodes by detection of negative-sense viral RNA. In conclusion, HuNoV GII.4 could induce acute gastroenteritis and replicate in the extra-intestinal lymphoid tissues in post-weaning gnotobiotic pigs. Therefore, such pigs would be a suitable animal model for studying the pathogenesis and replication of HuNoV.

• TTA Journal
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• s.35
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• pp.19-35
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• 1994

미국에서는 정보통신분야를 미래의 성장잠재력이 가장 큰 분야라고 보고 그 하부구조인 NII(National Information Infrastructure)로서 정보고속도로(Information Superhighway)를 건설하려고 한다. 고어부통령이 앞장서 제창하고 민간부문이 뒷받침하고 있는 이 구상은 고어자신이 부에노스아이레스에서 열린 세계전기통신개발회의(WDTC) 연설에서 밝힌 GII(Global Information Infrastructure)계획과 같이 앞으로 미국뿐만 아니라 전세계적으로도 현안 과제가 될 것이라고 생각된다. 따라서 민간부문을 중심으로 추진되어야 한다는 원칙을 표방하고 있는 미국의 NII구상을 살펴봄으로써 우리나라에서 추진될 초고속통신망의 모습을 미리 그려보는데 도움을 얻고자 한다.

• TTA Journal
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• s.36
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• pp.13-32
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• 1994

미국에서는 정보통신분야를 미래의 성장잠재력이 가장 큰 분야라고 보고 그 하부구조인 NII(National Information Infrastructure)로서 정보고속도로(Information Superhighway)를 건설하려고 한다. 고어부통령이 앞장서 제창하고 민간부문이 뒷받침하고 있는 이 구상은 고어자신이 부에노스아이레스에서 열린 세계전기통신개발회의(WDTC) 연설에서 밝힌 GII(Global Information Infrastructure)계획과 같이 앞으로 미국뿐만 아니라 전세계적으로도 현안 과제가 될 것이라고 생각된다. 따라서 민간부문을 중심으로 추진되어야 한다는 원칙을 표방하고 있는 미국의 NII구상을 살펴봄으로써 우리나라에서 추진될 초고속통신망의 모습을 미리 그려보는데 도움을 얻고자 한다.

• Proceedings of the Korean Society of Food Hygiene and Safety Conference
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• 2006.05a
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• pp.73-96
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• 2006

$\square$ A nationwide surveillance including 17 provincial labs has been established $\square$ Noroviruses are involved in recent viral gastroenteritis outbreaks and sproradic viral gastroenteritis cases in Korea $\square$ Analysis of RDRP and capsid regions of norovirus strains shows that various genotypes were circulated in Korea $\square$ Early detection of outbreaks cases (2004 Jeju-cause of epidemic) $\square$ New variant or emerging strain detection: variant GII4 and GIIb $\square$ Detection of Sapoviruses from AGE patients in 2003-4

• 정보화사회
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• s.100
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• pp.37-39
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• 1996

초고속정보통신망은 정보 서비스의 다양화, 정보통신의 대량화 및 정보서비스의 고속화에 대한 요구에 따라 대두된 정보 고속도로(Information Superhighway)의 개념이다. 정보고속도로 구축사업은 세계적인 정보화 추세에 따른 국가정보화의 하부구조(NII: National Information infrastructure)를 구축하는 것으로 미국에서는 초고속정보통신망 구축사업(GII: Global Information Infastructure)을 추진하고 있으며, 일본에서는 신사회 자본건설, 유럽연합에서는 고속행정통신망, 싱가폴에서는 IT-2000이라는 계획 아래 추진되고 있다.

• Korean Journal of Food Science and Technology
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• v.16 no.3
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• pp.322-328
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• 1984

These experiments were conducted to purify the glucoamylase produced by Rhizopus oryzae. Two forms of glucoamylase (GI and GII) from Phizopus oryzae were purified by $(NH_2)_2SO_4$ fractionation, acetone fractionation and successive column chromatography on DEAE-cellulose and CM-cellulose. The specific activities of GI and GII toward soluble starch were 157.6 U/㎎. protein (37.5 fold of crude extract), and 164.7 U/㎎. protein (39.2 fold of curde extract), respectively, and the yields of them were 4.3% and 3.8%, respectively. The two purified enzymes have shown a single band by polyacrylamide disc gel electrophoresis and SDS-polyacrylamide gel electrophoresis. The protein bands of their electrophoresis gel were revealed to have glucoamylase activity by iodine staining and were proved to be glycoprotein by periodic acid Schiff's staining.

• Journal of Audiology & Otology
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• v.25 no.2
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• pp.65-71
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• 2021

Background and Objectives: In masking level difference (MLD), the masked detection threshold for a signal is determined as a function of the relative interaural differences between the signal and the masker. Study 1 analyzed the results of school-aged children with good school performance in the MLD test, and study 2 compared their results with those of a group of children with poor academic performance. Subjects and Methods: Study 1 was conducted with 47 school-aged children with good academic performance (GI) and study 2 was carried out with 32 school-aged children with poor academic performance (GII). The inclusion criteria adopted for both studies were hearing thresholds within normal limits in basic audiological evaluation. Study 1 also considered normal performance in the central auditory processing test battery and absence of auditory complaints and/or of attention, language or speech issues. The MLD test was administered with a pure pulsatile tone of 500 Hz, in a binaural mode and intensity of 50 dBSL, using a CD player and audiometer. Results: In study 1, no significant correlation was observed, considering the influence of the variables age and sex in relation to the results obtained in homophase (SoNo), antiphase (SπNo) and MLD threshold conditions. The final mean MLD threshold was 13.66 dB. In study 2, the variables did not influence the test performance either. There was a significant difference between test results in SπNo conditions of the two groups, while no differences were found both in SoNo conditions and the final result of MLD. Conclusions: In study 1, the cut-off criterion of school-aged children in the MLD test was 9.3 dB. The variables (sex and age) did not interfere with the MLD results. In study 2, school performance did not differ in the MLD results. GII group showed inferior results than GI group, only in SπNo condition.