• Biomolecules & Therapeutics
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• v.3 no.4
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• pp.294-300
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• 1995

DA-3002, an authentic recombinant human growth hormone(rhGH), was examined for mutagenicity in the reverse mutation test on bacteria, in the chromosomal aberration test on cultured mammalian cells and in the micronucleous test on mice. The reverse mutation test was performed by a plate incorporation method with or without a metabolic activation system(S9 Mix) using Salmonella typhimurium strain TA100, TA1535, TA98 and TA1537. DA-3002 did not significantly increase revertant colonies in any of the test strains under any conditions at dose levels ranging from 0.0125 to 0.4 IU/plate, compared with the vehicle control. In the chromosomal aberration test using cultured Chinese hamster lung(CHL) cells, DA-3002 did not increase the number of aberrant cells in the presence or absence of S9 mix at concentrations of 0.0125 IU/mι to 0.4 IU/mι, compared with the vehicle control. In the micronucleus test, male ICR mice were given DA-3002 intraperitoneally at a dose level of 20, 40 and 80 lU/kg. The incidence of bone marrow micronucleated polychromatic erythrocytes in the DA-3002 treated mice did not differ from that of the vehicle control. These results indicate that DA-3002 doesn't have mutagenic potential under the present test conditions.

• Journal of Korean Society of Water and Wastewater
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• v.30 no.6
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• pp.635-643
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• 2016

Gas hydrate (GH)-based desalination process have a potential as a novel unit desalination process. GHs are nonstoichiometric crystalline inclusion compounds formed at low temperature and a high pressure condition by water and a number of guest gas molecules. After formation, pure GHs are separated from the remaining concentrated seawater and they are dissociated into guest gas and pure water in a low temperature and a high pressure condition. The condition of GH formation is different depending on the type of guest gas. This is the reason why the guest gas is a key to success of GH desalination process. The salt rejection of GH based desalination process appeared 60.5-93%, post treatment process is needed to finally meet the product water quality. This study adopted reverse osmosis (RO) as a post treatment. However, the test about gas rejection by RO process have to be performed because the guest gas will be dissolved in a GH product (RO feed). In this research, removal potential of dissolved gas by RO process is performed using lab-scale RO system and GC/MS analysis. The relation between RO membrane characteristics and gas removal rate were analyzed based on the GC/MS measurement.

• Clinical and Experimental Pediatrics
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• v.46 no.4
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• pp.363-369
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• 2003

Purpose : Pharmacologic provocation test of growth hormone(GH) is a non-physiologic method and has several limitations for diagnosing growth hormone(GH) deficiency. Spontaneous GH release studies could be important in understanding the pathophysiology of children with poor growth but normal responses to GH provocation tests. Also, the relationship between nocturnal GH secretions and sleep patterns in short stature children is poorly understood. The aim of this study is to determine whether there are differences in sleep patterns and nocturnal GH secretory profiles between idiopathic short stature children and a normal stature group. Methods : Spontaneous nocturnal GH secretions and sleep patterns were evaluated in 12 prepubertal idiopathic short stature children with normal responses to provocation tests and 9 normal stature controls. Blood samples were taken every 30 minutes from 22:00-06:30 and sleep patterns were analyzed by polysomnography. Results : The mean GH level during sleep was significantly lower in short stature children than in controls. The peak GH level after sleep, coincident with the first slow wave sleep, was lower in the short stature group. The slow wave sleep times of short stature children were decreased compared with those of normal subjects. Conclusion : These results suggest that overnight serial GH sampling is helpful to identify short stature children with subnormal GH secretions, and sleep structure differences may be associated with decreased overnight GH secretions in short stature children.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.21 no.2
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• pp.1-18
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• 2008

Objectives: Galgeunhaegitang-gamibang(GH) and Samhwangseze-gamibang(SG) has been known that they are helpful for treatment of atopic dermititis clinically, but there is no report about the effect of GH and SG. So, author aimed to investigate the effects of GH and SG on atopic dermititis of NC/Nga mice. Methods : NC/Nga mice were divide into three group : normal, control, and experimental group. Atopic dermatitis was induced in the control and experimental group by spreading DNCB. Then GH was orally administered three times in a week for 8 weeks to the experimental group and SG was spreaded two times in a day for 8 weeks to the experimental group, while the control group was given normal saline. We observed changes of clinical skin severity score, serum IgE, IL-4, IL-4, IL-5, IL-6, IgM, IgGl, $IFN-{\gamma}$ and so on. We used one-way ANOVA test statistically(p<0.01). Results : Clinical skin severities of experiment group in 13 and 16weeks were significantly decreased by 48% and 55% compared to the control group. Serum IgE, IL-4, IL-5, IL-6, IgM, IgGl levels of experimental group were singnificantly decreased compared to the control group. Serum $IFN-{\gamma}$ level of the experimental group was significantly increased against control group. mRNA expression levels of IL-4, IL-5, IL-6 and CCR3 in the skin tissues of experimental group were significantly decreased compared to the control group. In contrary, $IFN-{\gamma}$y mRNA expression level were increased compared to the control group. Histological observation of the ear and skin tissues showed that the extents of inflammation and infiltrated immune cells in the epidermis and dermis of experimental group were highly deminished compared to the control group. Judging from that $IL-1{\beta}$, $TNF-{\alpha}$, IL-6 expression of gene, the effects of inflammatory cytokine revelation were significantly decreased compared to the control group. In the model inducing COX-2 activity in RAW 264.7 cell, COX-2 activity was significantly inhibited depending on the density of GH compared to the control serum. According to cell multiplication, examination of cell toxicity showed that GH is safe at the density of 10, 50, 100mg/l and even 1000mg/l. Conclusion : Accordin to the above results, it is considered that GH and SG is effective treatment for the atopic dermatitis.

• Journal of Navigation and Port Research
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• v.32 no.10
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• pp.751-757
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• 2008

This paper made some statistical analyses on marine accidents in Korean waters using marine accident statistics. They were verified through analyses of one-way ANOVA: the difference in average number of marine accidents among Korean waters, between fishing and non-fishing vessels, and by year and by month The pairwise post hoc multiple comparisons of REGW-For GH test were additionally tested. As a result, there were significance differences among Korean waters. The difference of marine accidents by year was verified to have a statistical significance in the South and the East Sea for the fishing vessel, and in the South Sea for the non-fishing vessel. There were signifiant differences of monthly marine accidents by month in the Yellow and the East Sea for the fishing vessel only.

• Journal of Veterinary Science
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• v.21 no.1
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• pp.7.1-7.13
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• 2020

Aging triggers cellular and molecular alterations, including genomic instability and organ dysfunction, which increases the risk of disease in mammals. Recently, due to the markedly growing number of aging dogs in the world, as much as 49% in total number of pet dogs, it is necessary to improve and maintain their quality of life by understanding of the biological effects of aging. Therefore, the aim of this study was to determine specific biomarkers in aging dogs as a means of defining a set of hematological/biochemical biomarkers that influence the aging process. Blood samples were collected from younger (1-3 years) and older (7-10 years) dogs of middle/large size. The hematological/biochemistry analysis was performed to evaluate parameters significantly associated with age. Enzyme-linked immunosorbent assay was used to target growth hormone (GH)/insulin growth factor-1 (IGF-1), one of the main regulators of the aging process. Declining levels of total protein and increased levels of glucose in young dogs was observed regardless of their body size. Notably, a significantly high concentration of GH and IGF-1 in the younger dogs compared to the older dogs was found in middle/large-sized dogs. GH and IGF-1 were also found at significantly high levels in large-sized dogs compared to middle-sized dogs, suggesting a similar trend to that of elderly humans. Consequently, glucose, total protein, GH, and IGF-1 were identified as potential biomarkers for regulating the aging process in large/middle-sized dogs. These findings provide an invaluable insight into the mechanism of aging for the field of aging research.

• Journal of Life Science
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• v.19 no.11
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• pp.1644-1650
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• 2009

The purpose of this study was to investigate the effects of 12-week walking exercise on body composition, physical fitness, cardiovascular function, blood lipids, GH, IGF-1and leptin in obese and normal middle school girls. Participants included 12 pairs of middle school girls who were matched for sex, age and stratified by BMI (Obese Group: BMI$\geq$25.0; Normal Group: BMI<23.0). The variables of all factors were measured in all the subjects before and after the 12-week walking exercise program. The test data were analyzed by paired t-test, and t-test using the SPSS 14.0 statistics package and the alpha level of p<0.05 was set for all tests of significance. Body composition showed a significant difference in both groups, but the variables of physical fitness, cardiovascular function, blood lipids, growth hormone, and leptin did not show a significant difference in the two groups. We conclude that regular and continuous walking exercise improved body composition, physical fitness, cardiovascular function, blood lipids, and insulin factors in both groups. Consequently, these findings suggest that walking exercise will be effective for preventing obesity and improving health-related factors in the middle school girls.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1997.10a
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• pp.277-281
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• 1997

Reliab~l~ty englneerlng IS regarded as the major and important roll for all Industry And advanced nianufactur~ng systems w~th h~gh speed and lntell~gent ha\\ulcorner been dcvelop~ng for betterment of lnachln~ng db~l~ty In th~study, Me have rystem~~ed c\, ~luat~on c r f relidb~litl for nldchlntry system We proposed the rel~ab~l~ty asvxmcnt and dci~yned and manufactured r~ildb~llty tcst-bed to eualuate rcl~dbil~ty In ddd~t~on we acquired relldb~l~ty data uslng test-bed sydem and mde database to handle relrab~l~t) data And also he not only use rel~ab~l~ty data by andlyzlng rel~ab~l~ty, but also apply design revlew method usmg analycing crltlcal unlt5 of machlnery system From th~s stud), he u ~ l l expect to gu~de md Increase the rel~ab~l~t) englneerlng in devclop~ng and procerslng phase of h~gh qualrty product

• Korean Journal of Adult Nursing
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• v.17 no.5
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• pp.772-782
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• 2005

Objectives: The purpose of this study was to investigate the relationship between Korean lifestyle characteristics and health status and to identify the variables influencing health in Korea. Methods: A cross-sectional descriptive correlational design was used to explore the lifestyle characteristics and health status of 397 Korean adults. Correlational analysis calculated the correlation between lifestyle and health status. To examine the relationship among demographic characteristics, lifestyle, and health status we used the t-test and one-way ANOVA. Stepwise multiple regression was conducted to examine the significant predictors of general health among subjects. Results: Positive correlations were seen between general health (GH) and the overall score and subscales of the Lifestyle. The stepwise regression model showed that vitality (VA), body pain (BP), nutrition, and occupation were significant variables influencing general health (GH). Conclusions: These findings provide evidence regarding the lifestyle patterns and healthstatus among Koreans. When planning intervention strategies for this population, exercise and physical activity should be principal focus areas.

• Clinical and Experimental Pediatrics
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• v.49 no.6
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• pp.665-671
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• 2006

Purpose : To study the effects of growth hormone(GH) treatment on glucose metabolism and insulin resistance in children with idiopathic short stature(ISS). Methods : Glucose and insulin concentrations were measured during oral glucose tolerance test (OGTT) before and after GH treatment(0.6-0.7 IU/kg/week) in 20 patients with ISS. Insulin resistance was assessed by homeostasis model assessment(HOMA). Results : During OGTT, the mean blood glucose level did not show any significant changes after GH treatment. However, mean blood insulin levels of fasting and 30 minutes of OGTT showed significant increases after GH treatment, accompanying significant increases of insulin resistance. There was no difference in change of glucose, insulin levels and insulin resistance before and after GH treatment between two groups of body mass indices(BMI) of 25< and >25. There also was no significant difference between two groups of with and without family histories of diabetes mellitus (DM). There was no case of newly developed impaired glucose tolerance, fasting glucose tolerance, nor newly developed DM. Conclusion : GH treatment with doses of 0.6-0.7 IU/kg/week for mean 9.6 months in patients with ISS did not show any significant changes in blood glucose levels during OGTT. However, GH treatments induced considerably higher fasting insulin levels compared to pretreatment, resulting in statistically higher insulin resistance. Higher BMI and family history of DM did not induce any significant changes in glucose, insulin level and insulin resistance after GH treatment than the other groups.