• Journal of Climate Change Research
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• v.6 no.4
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• pp.303-310
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• 2015

This study was conducted to estimate of carbon stock and greenhouse gas (GHGs) removals by tree species and forest type at Gangwon province. We used a point sampling data with permanent sample plots in national forest inventory and national emission factors. GHGs emissions was caclulated using the stock change method related to K-MRV and IPCC guidance. Total carbon stock and greenhouse gas removals were high in deciduous forest and species than in coniferous. The range of annual net greenhouse gas emissions in other deciduous species was from $-11,564.83Gg\;CO_2\;yr^{-1}$ to $-13,500.60Gg\;CO_2\;yr^{-1}$ during 3 years (2011~2013). On the other hand, coniferous forest was temporally converted to source due to reducing of growing stock in 2012. It was that growing stocks and forest area were likely to reduce by the deforestation and clear cutting. This study did not consider other carbon pools (soil and dead organic matter) due to the lack of data. This study needs to complement the activity data and emission factors, and then will find the way to calculate the greenhouse gas emissions and removals in the near future.

• Journal of Gastric Cancer
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• v.22 no.4
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• pp.348-368
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• 2022

Purpose: Chromosomal instability is a hallmark of gastric cancer (GC). It can be driven by single nucleotide variants (SNVs) in cell cycle genes. We investigated the associations between SNVs in candidate genes, PLK2, PLK3, and ATM, and GC risk and clinicopathological features. Materials and Methods: The genotyping study included 542 patients with GC and healthy controls. Generalized linear models were used for the risk and clinicopathological association analyses. Survival analysis was performed using the Kaplan-Meier method. The binding of candidate miRs was analyzed using a luciferase reporter assay. Results: The PLK2 C_rs15009-C_rs963615 haplotype was under-represented in the GC group compared to that in the control group (P_corr=0.050). Male patients with the PLK2 rs963615 CT genotype had a lower risk of GC, whereas female patients had a higher risk (P=0.023; P=0.026). The PLK2 rs963615 CT genotype was associated with the absence of vascular invasion (P=0.012). The PLK3 rs12404160 AA genotype was associated with a higher risk of GC in the male population (P=0.015). The ATM T_rs228589-A_rs189037-G_rs4585 haplotype was associated with a higher risk of GC (P<0.001). The ATM rs228589, rs189037, and rs4585 genotypes TA+AA, AG+GG, and TG+GG were associated with the absence of perineural invasion (P=0.034). In vitro analysis showed that the cancer-associated miR-23b-5p mimic specifically bound to the PLK2 rs15009 G allele (P=0.0097). Moreover, low miR-23b expression predicted longer 10-year survival (P=0.0066) in patients with GC. Conclusions: PLK2, PLK3, and ATM SNVs could potentially be helpful for the prediction of GC risk and clinicopathological features. PLK2 rs15009 affects the binding of miR-23b-5p. MiR-23b-5p expression status could serve as a prognostic marker for survival in patients with GC.

• 중국학논총
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• no.62
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• pp.85-100
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• 2019

The article purpose is 'Reading Chinese translation text as a Korean integrated education for Chinese students'. Although number of foreign students has increased rapidly to the economic growth of Korea, the influence of Korean Wave, and the popularity of Korean popular culture like K-pop at domestic universities but the problems of their curriculum have been found in many places. Korean literary education through novel text has an important place in Korean studies, but literary education is often excluded in Korean language education as a foreign language education. Chinese students already have background knowledge of Korean translation novels through Chinese novels. They can get the learning effect as the Korean language study. Second, they can compared with Korean national violence and Chinese national violence through 'Red Revolution' and understand about Korean-Chinese understanding of the times, social and cultural phenomena, Third, they are able to study the theory of literature itself. also It was the educational purpose pursued by the humanities. Chinese students develop their Korean language skills by studying the Brothers which are translated into Korean, and we can see the similarities and differences of national violence by comparing Korea's '4.19' with China's 'Cultural Revolution' After comparing people, background, dynamics of the space where they are located, we can raise awareness of the historical and social problems of both countries. It is possible to study subjects' memories of space, change of local meaning, the formation of urban space or individual space in the text in the specific space where national violence occurs. In this way, the method of learning Korean integrated education through Brothers of the Chinese translation novels makes an opportunity to look at national violence in the Korean-Chinese space of the 1960s and 1970s. It has a subjective perspective from subordination to the nationality of the modern nation-state. This is an educational effect that can be obtained through reading a Chinese translation novel as a Korean language integrated education.

• Clinical Nutrition Research
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• v.12 no.1
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• pp.7-20
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• 2023

Creating a complex balance between dietary composition, circadian rhythm, and the hemostasis control of energy is important for managing diseases. Therefore, we aimed to determine the interaction between cryptochrome circadian clocks 1 polymorphism and energy-adjusted dietary inflammatory index (E-DII) on high-sensitivity C-reactive protein in women with central obesity. This cross-sectional study recruited 220 Iranian women aged 18-45 with central obesity. The 147-item semi-quantitative food frequency questionnaire was used to assess the dietary intakes, and the E-DII score was calculated. Anthropometric and biochemical measurements were determined. By polymerase chain response-restricted length polymorphism method, cryptochrome circadian clocks 1 polymorphism was assigned. Participants were categorized into three groups based on the E-DII score, then categorized according to cryptochrome circadian clocks 1 genotypes. The mean and standard deviation of age, BMI, and high-sensitivity C-reactive protein (hs-CRP) were 35.61 ± 9.57 years, 30.97 ± 4.16 kg/m², and 4.82 ± 5.16 mg/dL, respectively. The interaction of the CG genotype and E-DII score had a significant association with higher hs-CRP level compared to GG genotype as the reference group (β, 1.19; 95% CI, 0.11-2.27; p value, 0.03). There was a marginally significant association between the interaction of the CC genotype and the E-DII score with higher hs-CRP level compared to the GG genotype as the reference group (β, 0.85; 95% CI, -0.15 to 1.86; p value, 0.05). There is probably positive interaction between CG, CC genotypes of cryptochrome circadian clocks 1, and E-DII score on the high-sensitivity C-reactive protein level in women with central obesity.

• Applied Biological Chemistry
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• v.9
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• pp.111-118
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• 1968

A pot experiment with $P^{32}$ was carried out to investigate the soil phosphorus availability to four leguminous forage crops and three graminaceous, of black volcanic ash soil and red one. Soil phosphorus was extracted with 6 different extractants and also fractionated in Fe, Al and Ca phosphorus. The results were: 1) Soil phosphorus availability was in decreasing order of Italian rye grass${\gg}$ soybean> cassia> corn> weeping love grass${\gg}$ Korean lespedeza> Red clover and they might be grouped into three levels by A-value, over 1000, 200-500 and below 40 $p_{2}O_{5}\;kg/ha$. 2) The amount of various available phosphorus and phosoborus fraction in the black soil was higher than that in the red soil. No difference in phosphorus availabiliy to forage crops was shown between two soils. Therefore an extractant able to draw out similar amount of phosphorus from two soil will be suitable for determining the phosphorus availability index. 3) Two extractants, one extracting 20 ppm as maximum and the other extracting 100 ppm as minimum will be recommendable for determining the availability of phosphorus; the former for red clover and Lespedeza and the latter for others. Truog method may be good for the former but no appropriate method for the latter was found in the methods used. 4) T/R ratios of legumes were negatively correlated at 5% level with % phosphorus from fertilizer (% pdF). Legumes showed below 50 of % pdF over 5 of T/R ratio and over 80 of % pdF below 5 of T/R.

• Journal for History of Mathematics
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• v.27 no.2
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• pp.127-138
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• 2014

In this paper we investigate how Newton discovered the generalized binomial theorem. Newton's binomial theorem, or binomial series can be found in Calculus text books as a special case of Taylor series. It can also be understood as a formal power series which was first conceived by Euler if convergence does not matter much. Discovered before Taylor or Euler, Newton's binomial theorem must have a good explanation of its birth and validity. Newton learned the interpolation method from Wallis' famous book ${\ll}$Arithmetica Infinitorum${\gg}$ and employed it to get the theorem. The interpolation method, which Wallis devised to find the areas under a family of curves, was by nature arithmetrical but not geometrical. Newton himself used the method as a way of finding areas under curves. He noticed certain patterns hidden in the integer binomial sequence appeared in relation with curves and then applied them to rationals, finally obtained the generalized binomial sequence and the generalized binomial theorem.

• Journal of KSNVE
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• v.11 no.3
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• pp.428-436
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• 2001

The rotating inner cylinder executes a periodic translational motion in concentric annulus while the outer one is stationary. In the study of flow-induced vibrations and relaxed instabilities, it is of interest to evaluate the fluid-dynamic forces acting on the rotating inner cylinder. In the present work, the governing equations for the confined flow are expressed as Navier-Stokes equations, including the steady and unsteady terms. The fluid parameters for steady flow generated by the rotating cylinder are determined analytically while the unsteady ones by the oscillatory motion are evaluates by a numerical method based on the spectral collocation method. In order to validate the numerical approach, the numerical results are compared wish the analytical ones given by existing theories, for simple cases where the both approaches are applicable. Good agreement was found between the results. It is found the effects of the Reynolds number, defined by rotating velocity, on the fluid-dynamic forces are important for the case of relatively low oscillatory Reynolds number, defined by oscillatory frequency : j.e., in case of $Re_\omega\gg Re_S$.

• Korean Journal of Agricultural Science
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• v.47 no.1
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• pp.173-182
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• 2020

Human Astrovirus (HuAstV), known as a waterborne virus, is a group IV positive-sense single-stranded RNA that belongs to Astroviridae. The first outbreak of HuAstV was reported in England in 1975. HuAstV can exist not only among clinical patients but also in various water environments, such as water for agriculture and vegetables. For diagnosis of HuAstV from water samples, a polymerase chain reaction (PCR) system has been developed. However, the PCR-based diagnostic method has problems in field application, such as reaction time, sensitivity and specificity. For this reason, in this study we developed the loop-mediated isothermal amplification assay (LAMP) system, aimed specifically at HuAstV. Three prepared LAMP primer sets were tested by specificity, non-specificity and sensitivity; one LAMP primer set was selected with optimum reaction temperature. The developed LAMP primer set reaction conditions were confirmed at 62℃, and detection sensitivity was 1 fg/μL. In addition, restriction enzyme HaeIII (GG/CC) was introduced to confirm that the LAMP reaction was positive. As a result, selected LAMP primer set was 100 - 1000 times more specific, rapid, and sensitive than conventional-nested PCR methods. For verification of the developed LAMP assay, twenty samples of cDNA from groundwater samples were tested. We expect that the developed LAMP assay will be used to diagnose HuAstV from various samples.

• Journal of Animal Reproduction and Biotechnology
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• v.38 no.4
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• pp.209-214
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• 2023

Background: Single nucleotide polymorphisms (SNPs) are widely used genetic markers with applications in human disease diagnostics, animal breeding, and evolutionary studies, but existing genotyping methods can be labor-intensive and costly. The aim of this study is to develop a simple and rapid method for identification of a single nucleotide change. Methods: A modified Polymerase Chain Reaction Amplification of Multiple Specific Alleles (PAMSA) and high resolution melt (HRM) analysis was performed to discriminate a bovine polymorphism in the NCAPG gene (rs109570900, 1326T > G). Results: The inclusion of tails in the primers enabled allele discrimination based on PCR product lengths, detected through agarose gel electrophoresis, successfully determining various genotypes, albeit with some time and labor intensity due to the use of relatively costly high-resolution agarose gels. Additionally, high-resolution melt (HRM) analysis with tailed primers effectively distinguished the GG genotype from the TT genotype in bovine muscle cell lines, offering a reliable way to distinguish SNP polymorphisms without the need for time-consuming AS-PCR. Conclusions: Our experiments demonstrated the importance of incorporating unique mismatched bases in the allele-specific primers to prevent cross-amplification by fragmented primers. This efficient and cost-effective method, as presented here, enables genotyping laboratories to analyze SNPs using standard real-time PCR.

• Food Science of Animal Resources
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• v.34 no.5
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• pp.692-699
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• 2014

The aim of this study was to apply the external ionic gelation using an atomizing spray device comprised of a spray gun to improve the viability of Lactobacillus plantarum DKL 109 and for its commercial use. Three coating material formulas were used to microencapsulate L. plantarum DKL 109: 2% alginate (Al), 1% alginate/1% gellan gum (Al-GG), and 1.5% alginate/3% gum arabic (Al-GA). Particle size of microcapsules was ranged from 18.2 to $23.01{\mu}m$ depending on the coating materials. Al-GA microcapsules showed the highest microencapsulation yield (98.11%) and resulted in a significant increase in survivability of probiotic in a high acid and bile environment. Encapsulation also improved the storage stability of cells. The viability of encapsulated cells remained constant after 1-mon storage at ambient temperature. The external ionic gelation method using an atomizing spray device and the Al-GA seems to be an efficient encapsulation technology for protecting probiotics in terms of scale-up potential and small microcapsule size.