• The Korean Journal of Physiology and Pharmacology
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• v.21 no.1
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• pp.133-140
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• 2017

Conflicting evidence has been obtained regarding whether transient receptor potential cation channels (TRPC) are store-operated channels (SOCs) or receptor-operated channels (ROCs). Moreover, the Ca/Na permeability ratio differs depending on whether the current-voltage (I-V) curve has a doubly rectifying shape or inward rectifying shape. To investigate the calcium permeability of TRPC4 channels, we attached GCaMP6s to TRPC4 and simultaneously measured the current and calcium signals. A TRPC4 specific activator, (-)-englerin A, induced both current and calcium fluorescence with the similar time course. Muscarinic receptor stimulator, carbachol, also induced both current and calcium fluorescence with the similar time course. By forming heteromers with TRPC4, TRPC1 significantly reduced the inward current with outward rectifying I-V curve, which also caused the decrease of calcium fluorescence intensity. These results suggest that GCaMP6s attached to TRPC4 can detect slight calcium changes near TRPC4 channels. Consequently, TRPC4-GCaMP6s can be a useful tool for testing the calcium permeability of TRPC4 channels.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.25 no.1
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• pp.31-38
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• 1980

This experiment was carried out to study the heterosis and the GCA and SCA effects for days to heading of F 1 populations produced from eight wheat cultivar diallel. Greater heterosis for early heading was exhibited under the high temperature condition than under the low temperature conditions, and same results were also observed under short day condition rather than long day conditions. There are highly significant mean for GCA and SCA at all environmental conditions. Yecora F70 was showed the greatest effect, and Bezostaya and Blueboy were exhibited less effects of CCA for early heading. Significant GCA effect was expressed at high temperature and short day condition, indicating that this condition provides a better opportunity to select the materials for earliness. Selections of combination with greater effect of SCA for early heading such as Suweon 169 \times Yecora F70 were discussed for practical utilization.