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Optimizing In Vitro Propagation of Sophora koreensis Nakai using Statistical Analysis (다양한 통계분석 기법을 이용한 개느삼(Sophora koreensis Nakai)의 기내 증식 최적 조건 구명)

  • Jeong, Ukhan;Lee, Hwa;Park, Sanghee;Cheong, Eun Ju
    • Journal of Korean Society of Forest Science
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    • v.110 no.1
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    • pp.53-63
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    • 2021
  • Sophora koreensis Nakai is an indigenous plant in Koreawith a restricted natural range, part of which is in Gangwon province. The species is known to contain phytochemicals that have beneficial effects on human health, and it is economically important in bioindustry. Because of the limited number of plants in a small range of habitats, the mass-propagation method should be developed for use and conservation. In vitro tissue culture is a reliable method in terms of mass propagation from selected clones of the species. We investigated the optimal conditions of the medium in this process, especially focusing on the concentrations of plant growth regulators(PGRs) in the culture of stem-containing axillary buds. Three statistical methods, i.e., ANOVA, response surface method(RSM), and fuzzy clustering were used to analyze the plant growth, number of shoots induced, and shoot length with various combinations of PGRs. Results from the RSM differed from those of the other two methods; thus, the method was not suitable. ANOVA and fuzzy clustering showed similar results. However, more accurate results were obtained using fuzzy clustering because it provided a probability for each treatment. On the basis of the fuzzy clustering analysis, stem tissue produced the greatest number of shoots(11.03 per explant; 63.33%) on a medium supplemented with 5-��M 6-benzylaminopurine and 2.5-��M thidiazuron(TDZ). Proliferation of shoots(2.18 ± 0.21 cm, 63.33%) was attained on a medium supplemented with 2.5-��M BA, 2.5-��M TDZ, and 2.5-��M gibberellic acid.

Development of Estimation Equation for Minimum and Maximum DBH Using National Forest Inventory (국가산림자원조사 자료를 이용한 최저·최고 흉고직경 추정식 개발)

  • Kang, Jin-Taek;Yim, Jong-Su;Lee, Sun-Jeoung;Moon, Ga-Hyun;Ko, Chi-Ung
    • Journal of agriculture & life science
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    • v.53 no.6
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    • pp.23-33
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    • 2019
  • In accordance with a change in the management information system containing the management record and planning for the entire national forest in South Korea by an amendment of the relevant law (The national forest management planning and methods, Korea Forest Service), in this study, average, the maximum, and the minimum values for DBH were presented while only average values were required before the amendment. In this regard, there is a need for an estimation algorithm by which all the existing values for DBH established before the revision can be converted to the highest and the lowest ones. The purpose of this study is to develop an estimation equation to automatically show the minimum and the maximum values for DBH for 12 main tree species from the data in the national forest management information system. In order to develop the estimation equation for the minimum and the maximum values for DBH, there was exploited the 6,858 fixed sample plots of the fifth and the sixth national forest inventory between in 2006 and 2015. Two estimation models were applied for DBH-tree age and DHB-tree height using such growth variables as DBH, tree age, and height, to draw the estimation equation for the maximum and the minimum values for DBH. The findings showed that the most suitable model to estimate the minimum and the maximum values for DBH was Dmin=a+bD+cH, Dmax=a+bD+cH with the variables of DBH and height. Based on these optimal models, the estimation equation was devised for the minimum and the maximum values for DBH for the 12 main tree species.

Effect of Super Absorbent Polymer on Germination and Growth of Safflower and Amaranth Sprouts (고흡수성 합성고분자가 홍화 및 아마란스 새싹의 발아 및 생육에 미치는 영향)

  • Jang, Seong-Nam;Lee, Ga-Oun;Lee, Seung-man;Yun, Jae Gil;Shin, Hyunsuk;Son, Ki-Ho
    • Journal of Bio-Environment Control
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    • v.30 no.1
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    • pp.37-45
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    • 2021
  • This study was conducted to evaluate the growth characteristics, phenolic concentration and antioxidant capacity of safflower (Carthamus tinctorius L.) and amaranth (Amaranthus spp.) sprout and investigate the possibility of using super absorbent polymer (SAP) as a medium in hydroponic cultivation in a plant factory. The control was used a commercial sprout cultivation tool (19 × 14 × 9 cm, W × D × L), and a treatment (SAP) was added on the cultivation tool to compare the effect of SAP. Safflower sprouts were immersed in a distilled water at 30 ℃ for 5 hours, and then grown in a plant growth chamber. The temperature and relative humidity were maintained at 25 ± 1℃ and 70 ± 4%, respectively. The light condition was maintained at 35 ± 6 μmol·m-2·s-1 (12h). Amaranth sprouts were grown in a plant growth chamber maintained with temperature of 25 ± 2℃, relative humidity of 70 ± 5% and light condition of 188 ± 10 μmol·m-2·s-1 (16h). A physical and chemical characteristic of SAP, and a germination rate, growth characteristics and secondary metabolites were analyzed in both safflower and amaranth. There was no significant effect on SAP in a germination rate, growth and secondary metabolites of safflower compared to the control, whereas amaranth grown under SAP was higher in germination rate, dry weight, phenolic concentration, and antioxidant capacity compared to the control. As a result, this study was suggested that cultivation of sprouts using SAP would be possible in a plant factory, and further studies on SAP on plant physiological response are required.

Usability assessment of thermoplastic Bolus for skin VMAT radiotherapy (피부 병변에 대한 VMAT 치료 시 열가소성 bolus의 유용성 평가: case review)

  • Kim, Min Soo;Kim, Joo Ho;Shin, Hyun Kyung;Cho, Min Seok;Park, Ga Yeon
    • The Journal of Korean Society for Radiation Therapy
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    • v.32
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    • pp.85-92
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    • 2020
  • Purpose: To find out the advantages of thermoplastic bolus compared to conventional bolus, which is mainly used in clinical practice, We evaluated Two cases in terms of dose and location reproducibility to assess Usability of thermoplastic Bolus for skin VMAT radiotherapy. Materials and Methods: Two patient's treated with left breast skin lesion were simulated using thermoplastic Bolus and planned with 2arc VMAT. the prescription dose was irradiated to 95% or more of the target volume. We evaluated The reproducibility of the bolus position by measuring the length of the air gap in the CBCT (Cone Beam CT) image. to evaluate dose reproducibility, we compared The dose distribution in the plan and CBCT and measured in vivo for patient 2. Results: The difference between the air gap in patient 1's simulation CT and the mean air gap (M1) during 10 treatments in the CBCT image was -0.42±1.24mm. In patient 2, the difference between the average air gap between the skin and the bolus (M2) during 14 treatments was -1.08±1.3mm, and the air gap between the bolus (M3) was 0.49±1.16. The difference in the dose distribution between Plan CT and CBCT was -1.38% for PTV1 D95 and 0.39% for SKIN (max) in patient 1. In patient 2, PTV1 D95 showed a difference of 0.63% and SKIN (max) -0.53%. The in vivo measurement showed a difference of -1.47% from the planned dose. Conclusion: thermoplastic Bolus is simpler and takes less time to manufacture compared to those produced by 3D printer. Also compared to conventional bolus, it has high reproducibility in the set-up side and stable results in terms of dose delivery.

A Study of Test-Retest Reliability and Interrater Reliability of the Sensory Processing Scale for Children (SPS-C) (아동감각처리척도(Sensory Processing Scale for Children; SPS-C)의 검사-재검사 신뢰도와 검사자간 신뢰도 연구)

  • Kim, Kyeong-Mi;Kim, Ga-Yeon;Lee, Seung-Jin
    • The Journal of Korean Academy of Sensory Integration
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    • v.20 no.2
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    • pp.11-21
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    • 2022
  • Objective : This study examined the test-retest reliability and interrater reliability of the Sensory Processing Scale for Children (SPS-C). Method : Senventy primary caregivers of children with sensory processing difficulties and 3 years old participated in the study. The subjects were recruited through child development centers, welfare centers, and acquaintances located in Seoul, Gyeonggi-do, Busan, and Gyeongsang-do. The test-retest reliability verification targeted 20 main caregivers of children with difficulty in sensory processing. Re-evaluation was performed within 7 to 14 days after the initial evaluation, and Pearson's correlation coefficient was used to confirm the relevance between the two time points, and the Intraclass correlation coefficient was used to confirm the degree of agreement. The interrater reliability verification was conducted with 18 primary caregivers and 18 subsidiary caregivers of children with sensory processing difficulties. Each caregiver evaluated the same child, and the Intraclass correlation coefficient was used to confirm the agreement between the two sets of caregivers. Results : The test-retest reliability was Pearson's correlation coefficient r=.914 and intraclass correlation coefficient ICC=.939, indicating a high level of relevance and agreement. The interrater reliability was an Intraclass correlation coefficient ICC=.727, which showed a moderate level of agreement, but the tactile area (ICC=.455) and proprioceptive area (ICC=.439) were not statistically significant and showed a low degree of agreement. Conclusion : Through this study, it was confirmed that the children's Sensory Processing Scale for Children (SPS-C) is a stable evaluation tool with test-retest reliability and interrater reliability verified, and it will be able to provide help in standardization studies for future clinical use.

Evaluation of the Usefulness of Virtual Reality Equipment for Relieving Patients' Anxiety during Whole-Body Bone Scan (전신 뼈 검사 환자의 불안감 해소를 위한 가상현실 장비의 유용성 평가)

  • Kim, Hae-Rin;Kim, Jung-Yul;Lee, Seung-Jae;Baek, Song-Ee;Kim, Jin-Gu;Kim, Ga-Yoon;Nam-Koong, Hyuk;Kang, Chun-Goo;Kim, Jae-Sam
    • The Korean Journal of Nuclear Medicine Technology
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    • v.26 no.1
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    • pp.27-32
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    • 2022
  • Purpose When performing a whole-body bone scan, many patients are experiencing psychological difficulties due to the close distance to the detector. Recently, in the medical field, there is a report that using virtual reality (VR) equipment can give pain relief to pediatric patients with weak concentration or patients receiving severe treatment through a distraction method. Therefore, in this paper, VR equipment was used to provide psychological stability to patients during nuclear medicine tests, and it is intended to evaluate whether it can be used in clinical practice. Materials and Methods As VR equipment, ALLIP Z6 VR (ALLIP, Korea) was used and the experiment was conducted after connecting to a mobile phone. The subjects were 30 patients who underwent whole-body bone examination from September 1, 2021 to September 30, 2021. After intravenous injection of 99mTc-HDP, 3 to 6 hours later, VR equipment was put on and whole body images were obtained. After the test, a survey was conducted, and a Likert scale of 5 points was used for psychological anxiety and satisfaction with VR equipment. Hypothesis verification and reliability of the survey were analyzed using SPSS Statistics 25 (IBM, Corp., Armonk, NY, USA). Results Anxiety about the existing whole-body bone test was 3.03±1.53, whereas that of anxiety after wearing VR equipment was 2.0±1.21, indicating that anxiety decreased to 34%. When regression analysis of the effect of the patient's concentration on VR equipment on anxiety about the test, the B value was 0.750 (P<0.01) and the t value was 6.181 (P<0.01). decreased and showed an influence of 75%. In addition, overall satisfaction with VR equipment was 3.76±1.28, and the intention to reuse was 66%. The Cronbach α value of the reliability coefficient of the questionnaire was 0.901. Conclusion When using VR equipment, patients' attention was dispersed, anxiety was reduced, and psychological stability was found. In the future, as VR equipment technology develops, it is thought that if the equipment can be miniaturized and the resolution of VR content images is increased, it can be used in various clinical settings if it provides more realistic stability to the patient.

Effect of potato starch on suitability for 3D printing in golden threadfin bream (Nemipterus virgatus) surimi mixture preparation (감자 전분의 첨가가 수리미 혼합물 제조에서 3D 프린팅 적합성에 미치는 영향)

  • Seo, Hun-Seo;Park, Ye-Lin;Park, Jeong-Cheol;Han, Hyeon-Su;Kang, Yoo-Seok;Choi, Ye-Hui;Kim, Su-Hyeong;Kim, Han-Ho;Jeong, So-Mi;Kang, Woo-Sin;Kim, Su-Ryong;Ryu, Si-Hyeong;Lee, Ji-Eun;Xu, Xiaotong;Lee, Ga-Hye;Ahn, Dong-Hyun
    • Journal of Applied Biological Chemistry
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    • v.64 no.4
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    • pp.413-419
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    • 2021
  • In this study, we investigated the physical properties, color values of mixtures with starch from potato in preparing a cartridge for 3D printing using golden threadfin bream (Nemipterus virgatus) surimi as raw material. The results show that the hardness and gumminess of the surimi mixture added potato starch increased but cohesiveness decreased compared to the negative control. This means that the starch makes surimi stronger texture than the negative control. After 7 days of cold storage at 4 ℃, for unheated surimi, firmness is increased but adhesiveness is decreased. And among physical properties of surimi stored after heating, hardness and gumminess were decreased compared to before storage. Lastly, after storage for 7 days, the hardness and gumminess of fried surimi decreased compared to fried surimi before storage. As a result of sensory evaluation, texture, elasticity scent of surimi stored after heating were decreased but overall favorability was similar. Compared to the after storage, result of sensory evaluation of fried surimi, result of the before storage showed no significant differences. From these results, it is suggested that potato starch, which increases overall physical strength and have little effect on preference, can be used as an additive for golden threadfin bream surimi.

Effect of Pre-harvest Irradiation of UV-A and UV-B LED in Ginsenosides Content of Ginseng Sprouts (새싹 인삼의 수확 전 UV-A 및 -B LED의 조사에 의한 진세노사이드의 영향)

  • Jang, Seong-Nam;Lee, Ga-Oun;Sim, Han-Sol;Bae, Jin-Su;Lee, Ae-Ryeon;Cho, Du-Yong;Cho, Kye-Man;Son, Ki-Ho
    • Journal of Bio-Environment Control
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    • v.31 no.1
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    • pp.28-34
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    • 2022
  • This study was conducted to determine the changes in ginsenosides content according to additional UV-A, and UV-B LED irradiation before harvesting the ginseng sprouts. One-year-old ginseng seedlings (n=100) were transplanted in a tray containing a ginseng medium. The ginseng sprouts were grown for 37 days at a temperature of 20℃ (24h), a humidity of 70%, and an average light intensity of 80 µmol·m-2·s-1 (photoperiod; 24h) in a container-type plant factory. Ginseng sprouts were then transferred to a custom chamber equipped with UV-A (370 nm; 12.90 W·m-2) and UV-B (300 nm; 0.31 W·m-2) LEDs and treated for 3 days. Growth parameters and ginsenoside contents in shoot and root were conducted by harvesting on days 0 (control), 1, 2, and 3 of UV treatments, respectively. The growth parameters showed non-significant differences between the control and the UV treatments (wavelengths or the number of days). Ginsenoside contents of the shoot was highly improved by 186% in UV-A treatment compared to the control in 3 days of the treatment time. The ginsenoside contents of the roots was more improved in UV-A 1-day treatment and UV-B 3-day treatment, compared to the control by 171% and 160%, respectively. As a result of this experiment, it is thought that UV LED irradiation before harvesting can produce sprout ginseng with high ginsenoside contents in a plant factory.

Extraction and Analysis of Ganghwa Tidal Flat Channels Using TanDEM-X DEM (TanDEM-X DEM을 이용한 강화도 갯벌 조류로 추출과 분석)

  • Yun, Ga-Ram;Kim, Lyn;Kim, Nam-Yeong;Kim, Na-Gyeong;Jang, Yun-Yeong;Choi, Yeong-Jin;Lee, Seung-Kuk
    • The Journal of Engineering Geology
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    • v.32 no.3
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    • pp.411-420
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    • 2022
  • Recently, research using remote sensing has been active in various fields such as environment, science, and society. The results of research using remote sensing are not only numerical results, but also play an important role in solving and preventing social and scientific problems. The purpose of this thesis is to tell the correlation between the data provided and each data by using remote sensing technology for the tidal flat environment. The purpose of this study is to obtain high-resolution data using artificial satellites during remote sensing to find out information on tidal flat currents. Tidal flats created by erosion, sedimentation, low tide, and high tide contain information about the tidal flat slope and information about the ecosystem. Therefore, it can be considered as one of the very important studies to analyze the overall tidal flow channel. This paper creates a DEM (Digital Elevation Model) through TanDEM-X, and DEM is used as the most basic data to create a tidal channel. The research area is a tidal flat located in the middle of the west coast of Ganghwado tidal flat. By analyzing the tidal channel created, various information such as the slope direction of Ganghwado tidal flat and the shape of the tidal channel can be grasped. It is expected that the results of this study will increase the importance and necessity of using DEM data for tidal flat research in the future, and that high-quality results can be obtained.

Effect of Adding Milk on Compatibility with 3D Printing in the Preparation of a Surimi Mixture (수리미 혼합물 제조 시 우유 첨가에 따른 3D 프린팅 적합성에 미치는 영향)

  • Yoo-Seok Kang;Hye-ji Hwang;Ye-Lin Park;Hyeon-Su Han;Jeong-Cheol Park;Hun-Seo Seo;Ye-Hui Choi;Su-Hyeong Kim;Ka-Eun Woo;So-Mi Jeong;Ga-Hye lee;Dong-Hyun Ahn
    • Journal of Life Science
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    • v.33 no.5
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    • pp.391-396
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    • 2023
  • Milk is an emulsion, improving texture of surimi mixture and able to suppress off flavors and abnormal tastes. Therefore, this study aimed to identify the effective properties of milk in the preparation of a surimi mixture for 3D printing. The sensory and physical properties of surimi mixtures containing 0%, 20 wt%, and 40 wt% milk were evaluated, where the unheated surimi mixture with added milk demonstrated increased firmness and adhesiveness compared to the negative control group. In addition, the hardness, adhesiveness, gumminess, and chewiness of the mixture containing 40% milk were highest, but springiness, cohesiveness, and resilience were lowest. In the sensory evaluation, as the amount of milk increased, a fishy smell, abnormal taste and texture improved, hardness and preference increased as well. From these results, it was confirmed that a surimi mixture can be prepared with milk to improve its physical and sensory properties for 3D printing when compared to the negative control mixture. In particular, it was revealed that the physical properties and preference of the surimi mixture are best when prepared with 40% milk.