• Journal of the Korea Organic Resources Recycling Association
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• v.23 no.2
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• pp.28-35
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• 2015

In this study, the experiment was carried out to produce methane by applying Semi-Continuous Leachate Recirculation Anaerobic Digestion System fed with source separated food waste from school cafeteria. There were two systems and each system consisted of a bioreactor and a liquid tank. Each bioreactor had a screen near the bottom of the reactor. 2.5L of separated liquid was transferred to the liquid tank for 30min each day by using a tubing pump and the liquid from the liquid tank was pumped to the bioreactor at the upper of the bioreactor as soon as the transfer was ended. Through this circulation, the liquid having high concentration of VFAs was supplied to the top of bioreactor. At the beginning of the experiment, food waste/inoculum anaerobic sludge volume ratio was 2:8 that is 9g VS/L of OLR(Organic Loading Rate). Feeding was conducted every two weeks. Experimental results showed that the contents of moisture, combustible matter, ash were 65.91%, 32.73%, and 1.36%, respectively. Two different food waste loading were studied. The average organic loading rates were 3.51g VS/d for System A and 3.86g VS/d for System B, respectively. The average produced methane based on food waste fed to bioreactor were observed as $6.30m^3CH_4/kgVS{\cdot}d$ for system A and $4.94m^3CH_4/kgVS{\cdot}d$ for System B, respectively.

• Journal of Life Science
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• v.14 no.5
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• pp.840-846
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• 2004

A study for expression of Korean Mistletoe (KM) lectin gene (A,B) in Saccharomyces cerevisiae was done using transforming system of yeast. In order to overexpress the genes efficiently in yeast, two lectin genes (A,B) were re-cloned and modified including Kozak translation initiation sequence using PCR amplification. The constructed plasmids containing modified lectin A and B genes were transformed to S. cerevisea INVSc (MAT G, his3 $\Delta$1, leu2, trpl-289, ura3-52). The transformed cells were identified by DNA sequencing with ABI3700 system and induced with 2% of galactose for recombinant KM lectin (rKM lectin) protein. The rKM lectin A and B proteins were determinated about 29kDa size of protein by SOS-P AGE and western blotting analysis. The expressed recombinant lectin was determinated 1.24∼1.75 $\mu\textrm{g}$ per 1 mg of cytosolic soluble protein by sandwich ELISA method. Moreover the lectin genes were expressed as maximum level at 36 h after galactose induction and lectin A gene was were repressed after 48 h.

• BMB Reports
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• v.42 no.3
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• pp.154-159
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• 2009

JARID1B (jumonji AT rich interactive domain 1B) is a large nuclear protein that is highly expressed in breast cancers and is proposed to function as a repressor of gene expression. In this paper, a phage display screen using the N-terminus of JARID1B as bait identified one of the JARID1B interacting proteins, namely PcG protein (Polycomb group) hPc2. We demonstrated that the C-terminal region, including the COOH box, was required for the interaction with the N-terminus of JARID1B. In a reporter assay system, co-expression of JARID1B with hPc2 significantly enhanced the transcriptional repression. These results support a role for hPc2 acting as a transcriptional co-repressor.

• Journal of Acupuncture Research
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• v.30 no.1
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• pp.1-11
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• 2013

Objectives : The 7-zone-diagnostic system is a device for predetermining bodily locations by measuring the energy of the living body. The purpose of this study is to examine correlations between gastroscopy results and zone-5 data from the 7-zone-diagnostic system. Methods : This study was carried out with data from gastroscopy procedures. It involved 115 patients who had been diagnosed with gastritis, esophagitis, or gastric ulcers. These patients were divided into three groups according to the different patterns of zone-5 factors AA, FL2 and FL1. Group A was made up of patients for whom the red bar graph of zone-5 was higher than the normal range for factor AA. Group B was made up of patients for whom the red bar graph of zone-5 was within the normal range for factor AA. Group C was made up of patients for whom the red bar graph of zone-5 was lower than the normal range for factor AA. Group D E and F were made up of same way as group A B and C for factor FL2. Group G H and I were made up of same way as group A B and C for factor FL1. After the collection of gastroscopy results and data on zone-5 from the 7-zone-diagnostic system, the data was analyzed statistically. Results : 1. Group D had a higher ratio of medication than group E, and this result was statistically significant. 2. Group D G had a higher ratio of medication than group E G and group E H, and this result was statistically significant. 3. Group G had a higher ratio of esophagitis than group H, and this result was statistically significant. 4. A Group B H had a higher ratio of esophagitis than group B G, group C G, and group C H, and this result was statistically significant. 5. The medication group had a high score on the function index than the non-medication group, and this result was statistically significant. Conclusions : This study suggests that there is a slight correlation between gastroscopy results and zone-5 data from the 7-zone-diagnostic system. The research resulted in significant data that are helpful for diagnosing digestive system problems through the use of the 7-zone-diagnostic system.

• Korean Journal of Biological Psychiatry
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• v.11 no.2
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• pp.110-116
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• 2004

Objectives:Recently, polymorphisms of several serotonin genes have been suggested to be associated with suicide, but the results are still unclear. We examined whether the T102C polymorphisms of the serotonin 2A receptor gene and the G861C polymorphisms of the serotonin 1B receptor gene were associated with suicidal behavior using drug intoxication. Methods:The subjects were 52 patients who visited emergency room with suicidal behaviors. Fifty controls were selected from healthy volunteers matched for sex and age to the suicide subjects. The polymorphisms were analyzed with TaqMan$^{(R)}$ assay using primers based on previous studies. Results:The T102C polymorphism of the serotonin 2A receptor gene showed no significant difference between the suicidal attempters and controls in both genotype and allele frequency analyses(p=0.179 and p=0.422, respectively). There was no statistically significant difference between the suicidal attempters and the controls in the G861C polymorphism of the serotonin 1B receptor gene and any significant effect of the genotype distributions or the allele frequencies was not observed(p=0.092 and p=0.987, respectively). Conclusion:These findings suggest that the T102C polymorphism in serotonin 2A receptor gene and the G861C polymorphism in serotonin 1B receptor gene are not related to the susceptibility to suicide attempts using drugs. To clarify the genetic influences of the serotonergic system on suicidal behavior, the polymorphisms of other candidate genes in the serotonergic system should be studied with larger numbers of subjects.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.27 no.1
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• pp.21-30
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• 1991

The estimation of the fish biomass density or the size of fish by means of the acoustic equipment is an important part in the quantitative assessment of fisheries resources. The precision of such estimates depend upon the target strength of fish and the accuracy to which the acoustic equipment has been calibrated. This paper examine the accuracy of the digital measurement system which is manufactured by way of trial in order to masure the target strength of fish, and calibrations of that system carry out with an ogive and a ellipsoid made of the aluminum and the epoxy, respectively. Furthermore, measurements of target strength for eight species of fish are made at 25, 50, 100 kHz. The accuracy of the digital measurement system is compared the theory with measurements on ogive and ellipsoid, and the agreement is reasonable. Result of establishments on the target strength to fish length and to fish weight regression obtained from the measurements are available to provide the methods of design for use in interpreting acoustic measurements of fish abundance on the experimented eight species.

• Proceedings of the PSK Conference
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• 2005.11a
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• pp.231-232
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• 2005

Bacteria of Shigella spp. are responsible for shigellosis in humans, a disease characterized by destruction of the colonic epithelium that is induced by the inflammatory response elicited by invasive bacteria. They use a type III secretion system injecting effector proteins into host cells to induce their entry into epithelial cells and triggers apoptosis in macrophages. We present evidence that the effector OspG is a protein kinase that binds various ubiquitinylated ubiquitin-conjugating enzymes (E2s) and blocks degradation of phospho-$I{\kappa}B{\alpha}$ induced upon entry of bacteria into epithelial cells. Transfection experiments confirmed that OspG interferes with the $NF-{\kappa}B$ activation patway by preventing phospho-$I{\kappa}B{\alpha}$ degradation, suggesting that OspG inactivates a component of the $SCF^{{\beta}-TrCP}$ ubiquitin ligase complex (E3) involved in phospho-$I{\kappa}B{\alpha}$ ubiquitination. Upon infection of ileal loops in rabbits, the ospG mutant induced a stronger inflammatory response compared with the wild-type strain, indicating that OspG down-regulates the host innate response induced by invasive bacteria.

• Journal of Advanced Navigation Technology
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• v.24 no.6
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• pp.601-606
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• 2020

In this paper, we designed a microstrip patch antenna that can be applied to the Wimax/LTE 5G system among wireless media usable in coastal ships. The substrate of the proposed antenna is FR-4 (er=4.3), the size is 22 mm × 30 mm, and it can be used in the 3.5 GHz and 5.8 GHz bands of Wimax/LTE 5G by constructing a simple structure using a microstrip patch antenna. CST Microwave Studio 2014 was used for simulation, and the gain of the simulation result is 2.41dB at 2.4 GHz and 3.96 dB at 3.5 GHz. S-Parameter also showed a result of less than -10 dB (VSWR 2:1) in the desired frequency band, and designed a small variable and a miniaturized antenna so that the antenna can be used in mobile phones or electronic devices.

• 한국IT서비스학회:학술대회논문집
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• 2002.11a
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• pp.327-333
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• 2002

현재까지 e-비즈니스와 관련된 많은 자료들과 솔루션들이 발표되었지만 기업을 위한 e-비즈니스 환경에 IT 기술을 접목시켜 기업의 내부의 IT 기술과 솔루션에 대한 연구가 활발하지 않은 듯 하다. e-비즈니스의 유형은 기업과 고객(B2C), 기업과 기업(B2B), 국가와 기업(G2B), 국가와 국가(G2G) 등으로 세분화 할 수 있으나, 가장 중심적 역할을 하는 주체는 기업 조직이라고 하겠다. 본 연구에서는 많은 e-비즈니스의 구현 기반 기술 중에서 기업 내부 측면의 e-비즈니스 구현 기반기술과 관련된 IT 기술들은 전사적 자원관리를 위한 ERP(Enterprise Resource Planning)와 지식 기반의 정보 분석을 위한 DW/CRM(Data Warehouse/ Customer Relationship Management), 기업내부의 정보 통로인 EIP(Enterprise Information Portal}, 기업내부의 이기종 어플리케이션간의 통합을 위한 EAI(Enterprise Application Integration)와 경영층의 전략적인 의사결정을 지원하기 위한 SEM(Strategy Executive Management), 확장 ERP 개념인 SCM(Supply Chain Management) 기술에 대한 개념적 이해와 기업내부의 e-비즈니스 환경을 위한 적용방안을 밝히고 각 IT별 솔루션의 분류함에 의해 기업의 e-비즈니스 시스템의 구현 방향을 제시하고자 한다.

• International Journal of Industrial Entomology and Biomaterials
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• v.2 no.2
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• pp.185-189
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• 2001

Wild type Bacilus thuringiensis subsp. israelensis and B. sphaericus toxins have been used separately as active in ingredients for bacterial insecticides to control mosquito larvae due to their comparable toxicity to chemical insecticides. Cry11B, recently cloned from B. thuringiensis subsp. jegathesan, shows higher toxicity against three major species of mosquito larvae than Cry11A, one of the major component of B. thuringiensis subsp. israelensis inclusion body. To determine whether the combination of cry11B and B. sphaericus binary toxins is as toxic as B. thuringiensis subsp. israelensis parental strain, cry11B and B. sphaericus binary toxins genes were co-expressed as an operon using cytlA promoters/STAB-SD hybrid expression system in B. thuringiensis subsp. israelensis acrystalliferous strain 4Q7. However, unexpectedly, B. sphaericus binary toxins were barely produced, whereas relatively large amount of Cry11B was produced. When this strain was grown in four different media, NB+G and Peptonized Milk produced more toxin proteins and spores per unit of media than GYS and G-Tris. Toxicity of this strain against fourth instar Culex quinquefasciatus was ranged from of 8.3 to 45.7 ng/ml, with NB+G culture being the highest, and GYS culture was the lowest.