• Title/Summary/Keyword: G134A

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성장발육에 따른 골 백서경조직의 무기질함량의 변화

  • Yoo, Kwang-Hee
    • The Journal of the Korean dental association
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    • v.12 no.4
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    • pp.269-273
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    • 1974
  • This present investigation was concerned with the content of ash, Mg, Ca, K and N'a of bone, cartilage, incisor teeth and molar teeth in rats of different weights. The results obtained were summarized as follows. 1. Cartilage is the most adequate model for the study in vivo of mechanisms concerned with normal calcification. 2. The percentage of inorganic material in cartilage rose from approx 13% of the dry weight in 5g rat to 46% in the tissue from rats weighing 134g 3. There was no marked change in the content of ash, Ca, Mg, K and Na after eruptions due to the increasing weight.

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Preparation and Release Behavior of Methoxy poly(ethylene glycol)- poly(L-lactide-co-glycolide) Wafer Containing Albumin (알부민을 함유한 메톡시 폴리(에틸렌 글리콜)- 폴리(L-락타이드-co-글리콜라이드) 웨이퍼의 제조 및 방출거동)

  • 서광수;김문석;김경자;조선행;이해방;강길선
    • Polymer(Korea)
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    • v.28 no.4
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    • pp.328-334
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    • 2004
  • A series of methoxy poly(ethylene glycol) (MPEG)-poly(L-lactide-co-glycolide) (PLGA) diblock copolymers were synthesized by ring-opening polymerization of L-lactide and glycolide with carbitol (134 g/mole) or different molecular weights of MPEG (550, 2000, and 5000 g/mole) as an initiator in presence of Sn(Oct)$_2$. The properties of diblock copolymers were characterized by using $^1$H-NMR, GPC, and XRD. After uniform mixing of block copolymers and 1% albumin bovine-fluorescein isothiocyanate(FITC-BSA) with a freeze miller, the wafers loaded FITC-BSA were fabricated by using a mold with a dimensions of 3 mm${\times}$1mm diameter. The release profiles of FITC-BSA and the pH changes of wafer were examined using pH 7.4 PBS for 30 days at 37$^{\circ}C$. The release profiles of albumin showed fast initial burst as the molecular weights of MPEG increased. As a result of this study, the release behavior of BSA was controlled with introducing MPEG in the block copolymers.

Effects of Insulin on Acetyl-CoA Carboxylase in Bovine Mammary Secretory Cells

  • Kim, W.Y.;Ha, J.K.;Baldwin, R.L.
    • Asian-Australasian Journal of Animal Sciences
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    • v.10 no.1
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    • pp.134-140
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    • 1997
  • Objectives of this study were to determine effects of insulin on acetyl-CoA carboxylase (ACC) activity and correlate this activity with relative amounts of ACC in MAC-T cells. MAC-T cells were grown in Medium 199 supplemented with fetal bovine serum (5%), cortisol ($1{\mu}g/ml$), and insulin ($1{\mu}g/ml$). At confuluence, the cells were transferred to $100mm^2$ culture dishes coated with the extracelluar matrix. After 10 h of incubation, the media were replaced with media without fetal bovine serum and the concentration of insulin was lowered to 5 ng/ml. After 24 h, the media were changed to contain the varying concentrations of insulin and incubations continued for 48 h. The addition of insulin resulted in increases in the specific activity of ACC. The maximal effects of insulin on the ACC activity occurred at concentrations of insulin, 1,000 ng/ml. In contrast, the relative change in lactate dehydrogenase (LDH) activity in response to increasing insulin concentration was minimal as compared to the effects of insulin on ACC. Transblot and enhanced chemiluminescence (ECL) analysis indicated that the increase in ACC activity in MAC-T cells caused by insulin were due to actual increases in amounts of enzyme.

METAL RELEASE FROM BRACKETS AND ARCHWIRES (BRACKET과 호선의 금속유리)

  • Park, Soo Byung;Rhee, Byung Tae
    • The korean journal of orthodontics
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    • v.19 no.2
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    • pp.75-84
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    • 1989
  • The purpose of this experiment was to measure the amounts of nickel and chromium released from a simulated orthodontic appliance in artificial saliva. Simulated mandibular half-arch orthodontic appliances were composed of American Iron and Steel Institute type 304 brackets, Permachrome, Elgiloy, Australian wire, T.M.A. and Nitinol. The amounts of nickel and chromium released from sample were measured with atomic absorption spectrophotometer after 3, 6, 9, 12, 15 days. The cumulative amounts of nickel released from Permachrome, Elgiloy, Australian wire and T.M.A. reached a plateau after 6 days. But the cumulative amounts of nickel released from Nitinol increased continuously. Significant amounts of both nickel and chromium were solubilized from the simulated orthodontic appliances into artificial saliva. After 15 days, total cumulative amounts of nickel were $152.15{\mu}g$, $150.27{\mu}g$, $134.74{\mu}g$, $114.67{\mu}g$ and $93.39{\mu}$ from the Elgiloy, Australian wire, Nitinol, Permachrome and T.M.A., respectively. Total cumulative amounts of chromium from Permachrome, Australian wire, Elgiloy, Nitinol and T.M.A. were $100.83{\mu}g$, $83.64{\mu}g$, $81.61{\mu}g$, $14.90{\mu}g$ and $12.43{\mu}g$, respectively. The result showed that nickel released from Elgiloy and chromium released from Permachrome were more than any others.

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Breeding of Productive Bivoltine Hybrid, CSR16${\times}$CSR17 of Silkworm Bombyx mori L.

  • Reddy, N.Mal;Basavaraja, H.K.;Kumar, N.Suresh;Joge, P.G.;Kalpana, G.V.;Dandin, S.B.;Datta, R.K.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.8 no.2
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    • pp.129-133
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    • 2004
  • The breeding work was initiated by utilizing two Japanese hybrids namely C135${\times}$N134 and N137${\times}$C146 along with two evolved Indian breeds, J14 and A24. The breed CSR16 which is characterized with marked larvae and white dumbbell cocoons was evolved from the Japanese hybrid C135${\times}$N134 by crossing with J14, while the breed CSR17 which is characterized with plain larvae and white oval cocoons was evolved from the Japanese hybrid N137${\times}$C146 by crossing with A24. The hybrid of these evolved breeds i.e., CSR16${\times}$CSR17 has shown superior over control hybrid $KA{\times}{NB_4}{D_2}$ and on par with the ruling hybrid of CSR2${\times}$CSR4. The hybrid CSR16${\times}$CSR17 was authorized during 1999 by Central Silk Board, Bangalore, Government of India for commercial exploitation during favourable months based on national level race authorization test.

A Cipadesin Limonoid and a Tirucallane Triterpene from the Fruit of Sandoricum koetjape and their Inhibitory Properties against Receptor Tyrosine Kinases

  • Rachmadhaningtiyas, Dyah Ayu;Heliawati, Leny;Hermawati, Elvira;Syah, Yana Maolana
    • Natural Product Sciences
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    • v.27 no.2
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    • pp.134-139
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    • 2021
  • A new cipadesin limonoid, i.e. 3-epi-cipadonoid C (1), and a new tirucallane triterpene, i.e. hispidol B 3-palmitate (3), have been isolated from the seeds and fruit peels extract of Sandoricum koetjape, respectively. Along with these compounds the known limonoid, cipaferen G (2), and two pentacyclic triterpenes, bryonolic (4) and bryononic (5) acids, were also isolated. The strucrures of the new compounds were elucidated by the analysis of NMR and mass spectral data. Compounds 1 - 5 were evaluated as the inhibitor of receptor tyrosine kinases (EGFR, Epidermal Growth Factor Receptor; HER2, HER4, Human Epidermal growth factor Receptor 2, -4; IGFR, Insulin-like Growth Factor Receptor; InsR, Insulin Receptor; KDR, Kinase insert Domain Receptor; PDGFRα, and PDGFRβ, Platelet-Derived Growth Factor Receptor-α and -β). The results showed only 1 and 3 that have weak activity against InsR.

Simultaneous analysis of ethylene glycol and glycolic acid in bio-specimens by GC/MS (생체시료에서 GC/MS에 의한 에틸렌글리콜 및 대사체인 글리콜산 동시분석)

  • Lee, Joon-Bae;Park, Mee-Jung;Sung, Tae-Myung;Choi, Byung-Ha;You, Jae-Hoon;Shon, Shung-Kun;Paeng, Ki-Jung
    • Analytical Science and Technology
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    • v.23 no.6
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    • pp.544-550
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    • 2010
  • Mistaking pink colored thermal oil for grape wine, a victim drank the oil to death which was analyzed to contain 39% of ethylene glycol. Thermal oil could be used for heat transfer to prevent the malfunction due to the high pressure in the boiler operated at high temperature when using water. Main component of thermal oil is known to be mineral oil or ethylene glycol. From the blood and other tissue of the victim from autopsy, ethylene glycol and its metabolite were simultaneously analyzed by GC/MS after extraction under acidic condition with acetonitrile followed by derivatization with BSTFA. About 0.2 g of the specimens were pretreated with 50 uL of 0.5 M HCl solution to keep acidic condition, then dehydrated with anhydrous sodium sulfate followed by concentration under nitrogen stream. Ethylene glycol and glycolic acid concentration in blood was measured to be $2,755\;{\mu}g/mL$ and $174\;{\mu}g/mL$ respectively. In other specimen, the concentration of ethylene glycol and glycolic acid was $860\;{\mu}g/g\sim1,290\;{\mu}g/g$ and $93\;{\mu}g/g\sim134\;{\mu}g/g$. Especially, crystal appeared in kidney which was supposed xalate from the metabolite of ethylene glycol.

Object Detection Using Deep Learning Algorithm CNN

  • S. Sumahasan;Udaya Kumar Addanki;Navya Irlapati;Amulya Jonnala
    • International Journal of Computer Science & Network Security
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    • v.24 no.5
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    • pp.129-134
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    • 2024
  • Object Detection is an emerging technology in the field of Computer Vision and Image Processing that deals with detecting objects of a particular class in digital images. It has considered being one of the complicated and challenging tasks in computer vision. Earlier several machine learning-based approaches like SIFT (Scale-invariant feature transform) and HOG (Histogram of oriented gradients) are widely used to classify objects in an image. These approaches use the Support vector machine for classification. The biggest challenges with these approaches are that they are computationally intensive for use in real-time applications, and these methods do not work well with massive datasets. To overcome these challenges, we implemented a Deep Learning based approach Convolutional Neural Network (CNN) in this paper. The Proposed approach provides accurate results in detecting objects in an image by the area of object highlighted in a Bounding Box along with its accuracy.

The effect of laser energy on the preparation of iron oxide by a pulsed laser ablation in ethanol

  • Maneeratanasarn, P.;Khai, T.V.;Choi, B.G.;Shim, K.B.
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.22 no.3
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    • pp.134-138
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    • 2012
  • Recently the preparation magnetic nanoparticles by a pulsed laser ablation in liquid has gained much attention because it is easy to control experimental parameters. Iron oxide magnetic nanoparticles have been prepared by a pulsed laser ablation of ${\alpha}-Fe_2O_3$ target in ethanol at different magnitude of laser energy of 1, 20, 40 and 80 mJ/pulse. It revealed that particle size increases with increasing laser energy. It could be concluded that 40 mJ/pulse is an optimum laser energy for the preparation of iron oxide nanoparticles with uniform size distribution. The nanoparticles are homogeneously dispersed in ethanol and their stability maintained for several months.

Detection of IgG and IgM antibodies with ELISA technique in human trichomoniasis (질트리코모나스 환자에서 효소표식 면역검사법을 이용한 혈청 내 항-질트리코모나스 IgG 및 IgM 항체가의 측정)

  • 이미리;신명헌
    • Parasites, Hosts and Diseases
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    • v.28 no.1
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    • pp.25-30
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    • 1990
  • The direct wet mount examination of vaginal. secretion, widely applied for the diagnosis of Trichcmonas vaginalis infection in woman patients, is rapi4 and economical, however, the sensitivity of this technique is not so high. In this study enzyme-linked immunosorbent assay (ELISA) was employed for the detection of serum anti-T. vaginalis IgG and IsM antibodies from 30 vaginal trichomoniasis patients and 30 non-infected healthy persons. The results were as follows: 1. Serum ELISA-IgG value was $0.37{\pm}0.134(Mean{\pm}S.D.)$ in vaginal trichomoniasis patients and $0.21{\pm}0.054$ in healthy controls(P<0.005), and the sensitivity and specificity of ELISA for serum IgG antibody were 70.0% and 96.7%, respectively. 2. Serum ELISA-IgM value was $0.33{\pm}0.177 (Mean{\pm}S.D.)$ in vaginal trichomonlasls patients and $0.11{\pm}0.051$ in healthy controls (p<0.005), and the sensitivity and specificity of ELISA for serum IsM antibody were 70.0% and 96.7%, respectively. 3, The ELISA-IgG values showed a significant correlation with ELISA-IgM values(r=0.77, p<0.005) , With above results, it is assumed that ELISA is a reliable method for the diagnosis of T vaginalis infection and simultaneous measurement of serum IgG and IgM with this technique is recommended.

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