• Asian-Australasian Journal of Animal Sciences
• /
• 제26권6호
• /
• pp.838-844
• /
• 2013

This study was conducted to evaluate chemical composition, nitrogen-corrected true metabolizable energy (TMEn) and true amino acids digestibility of corn distillers dried grains with solubles (DDGS) produced in China. Twenty five sources of corn DDGS was collected from 8 provinces of China. A precision-fed rooster assay was used to determine TMEn and amino acids digestibility with 35 adult cecectomized roosters, in which each DDGS sample was tube fed (30 g). The average content of ash, crude protein, total amino acid, ether extract, crude fiber and neutral detergent fiber were 4.81, 27.91, 22.51, 15.22, 6.35 and 37.58%, respectively. TMEn of DDGS ranged from 1,779 to 3,071 kcal/kg and averaged 2,517 kcal/kg. Coefficient of variation for non-amino acid crude protein, ether extract, crude fiber and TMEn were 55.0, 15.7, 15.9 and 17.1%, respectively. The average true amino acid digestibility was 77.32%. Stepwise regression analysis obtained the following equation: TMEn, kcal/kg = -2,995.6+0.88${\times}$gross energy+$49.63{\times}a^*$ (BIC = 248.8; RMSE = 190.8; p<0.01). Removing gross energy from the model obtained the following equation: TMEn, kcal/kg = 57.88${\times}$ether extracts+$87.62{\times}a^*$ (BIC = 254.3, RMSE = 223.5; p<0.01). No correlation was found between color scores and lysine true digestibility (p>0.05). These results suggest that corn DDGS produced in China has a large variation in chemical composition, and gross energy and $a^*$ value can be used to generate TMEn predict equation.

• Asian-Australasian Journal of Animal Sciences
• /
• 제22권7호
• /
• pp.943-954
• /
• 2009

Diacylglycerol acyltransferase (DGAT) is a key enzyme that catalyzes the final and rate-limiting step of triglyceride synthesis. Both DGAT1 and DGAT2 genes code proteins with DGAT activity. Studies have shown DGAT1 polymorphisms associate with intramuscular fat deposition in beef cattle, but fewer associations between DGAT2 and beef cattle economic traits have been reported. The objective of this study was to investigate single nucleotide polymorphism (SNP) in intron3 of bovine DGAT2 and evaluate the associations of that with carcass, meat quality, and fat yield traits. Test animals were 157 commercial feedlot steers belonging to 3 Chinese native breeds (22 for Luxi, 24 for Jinnan, and 23 for Qinchuan), 3 cross populations (20 for Charolais${\times}$Fuzhou, 18 for Limousin ${\times}$Luxi, and 17 for Simmental${\times}$Jinan) and 1 Taurus pure breed population (16 Angus steers). In the current study, 15 SNP were discovered in intron3 and exon4 of DGAT2 at positions 65, 128, 178, 210, 241, 255, 270, 312, 328, 334, 365, 366, 371, 415, and 437 (named as their positions in PCR amplified fragments). Only 7 of them (128, 178, 241, 270, 312, 328, and 371) were analyzed, because SNP in three groups (65-128-255, 178-210-365 and 241-334-366) were in complete linkage disequilibrium within the group, and SNP 415 was a deletion and 437 was a null mutation. Frequencies for rare alleles in the 3 native breed populations were higher than in the 3 cross populations for 178 (p = 0.04), 270 (p = 0.001), 312 (p = 0.03) and 371 (p = 0.002). A general linear model was used to evaluate the associations between either SNP genotypes or allele substitutions and the measured traits. Results showed that SNP 270 had a significant association with the fat yield associated with kidney, pelvic cavity, heart, intestine, and stomach (KPHISY). Animals with genotype CC and CT for 270 had less (CC: -7.71${\pm}$3.3 kg and CT: -5.34${\pm}$2.5 kg) KPHISY than animals with genotype TT (p = 0.02). Allele C for 270 was associated with an increase of -4.26${\pm}$1.52 kg KPHISY (p = 0.006) and $-0.92{\pm}0.45%$ of retail cuts weight percentage (NMP, Retail cuts weight/slaughter body weight) (p = 0.045); allele G for 312 was associated with an increase of -5.45${\pm}$2.41 kg KPHISY (p = 0.026). An initial conclusion was that associations do exist between DGAT2 gene and carcass fat traits. Because of the small sample size of this study, it is proposed that further effort is required to validate these findings in larger populations.

• 환경영향평가
• /
• 제24권4호
• /
• pp.352-366
• /
• 2015

본 연구는 병아리꽃나무 자생지의 식물상, 식생구조 및 토양과 같은 생태적 특성을 조사 및 분석하여 자생지 보전과 관리를 위한 기초 자료를 제공하는데 목적이 있다. 식물상은 57과 99속 107종 1아종 17변종 6품종 등 총 131분류군이며, 생활형은 교목(MM) 21분류군, 아교목(M) 24분류군, 관목(N) 30분류군, 지표식물(Ch) 6분류군, 반지중식물(H) 20분류군, 지중식물(G) 17분류군, 일년생식물(Th) 12분류군, 수생식물(HH) 1분류군이었다. 군락유형은 느티나무군락, 갈참나무군락, 참느릅나무군락, 까마귀베개군락, 밤나무군락, 자귀나무군락, 팽나무군락, 아까시나무군락 등 8개이다. 층위별 우점종의 경우 교목층은 느티나무, 갈참나무, 참느릅나무, 밤나무, 자귀나무, 팽나무, 아까시나무, 아교목층은 까마귀베개, 갈참나무, 느티나무, 자귀나무, 참느릅나무, 닥나무, 관목층은 병아리꽃나무로 나타났다. 종다양도 분석 결과, 종다양성지수는 1.691~2.610, 최대종다양성지수는 2.197~3.466, 균재도는 0.646~0.903, 우점도는 0.097~0.354로 나타났다. 토양 분석 결과, 평균 산도는 5.6, 평균 전기전도도 0.5ds/m, 평균 유효인산 59.1mg/kg, 평균 유기물함량 49.7%, 평균 치환성칼륨 $0.4cmol^+/kg$, 평균 치환성칼슘 $13.5cmol^+/kg$, 평균 치환성마그네슘 $3.3cmol^+/kg$으로 분석되었다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제23권11호
• /
• pp.1427-1435
• /
• 2010

The present study was conducted to examine the effect of a stair-stepped feed intake pattern on growth, feed efficiency, and meat quality of Hanwoo steers. Twenty-seven 11-month-old Hanwoo steers were randomly divided into three groups. The control group was fed according to the Korean steer feeding program, and the other two groups were fed according to an alternated feeding schedule of 3-2-4-2 months. During the first three months of the experiment, treatment group 1 (T1) and treatment group 2 (T2) were fed 20% and 30% less than the control group, respectively. For the following two months, the T1 group was fed 20% more than the control group while the T2 group was fed 20% less than the control group. In the third step, T1 and T2 groups were fed 20% and 10% less, respectively, than the control group for four months. In the last two months, T1 and T2 groups were fed 20% more than the control group. After the stair-step feeding trial, steers were fed concentrated feed ad libitum for five months. The altered feed intake pattern did not affect daily body weight gain. However, daily feed intake tended to decrease and growth efficiency tended to increase in the two treatment groups compared to the control group. Altered feed intake also affected blood metabolite levels. The serum glucose and BUN level of the T1 group increased in the first re-fed period compared to the T2 and control groups. The serum cholesterol level of the T2 group decreased in the first restricted-re-fed growth period compared to the T1 and control groups. The serum NEFA levels of the two treatment groups increased from the first restricted period compared to the controls. The serum insulin level of the T2 group increased in the last period compared to the T1 and control groups. Regarding meat yield index, the control group was significantly higher than the T2 group (p<0.05). Regarding meat yield grade, the carcass back fat thickness of the T2 group was significantly higher than the control group (p<0.05). In marbling score, the T1 group was the highest (4.9), followed by the control group (4.1) and the T2 group (4.0). These results indicate that using a stair-stepped growth pattern (T1) can contribute to improvements in growth efficiency and muscle marbling.

• Asian-Australasian Journal of Animal Sciences
• /
• 제25권2호
• /
• pp.267-271
• /
• 2012

An experiment was conducted to evaluate the effects of one alpha-hydroxycholecalciferol ($1{\alpha}$-OH $D_3$) on growth performance, tibia quality, and skin and meat color in broilers fed calcium (Ca)- and phosphorus (P)-deficient corn-soybean meal diets. A total of 288 male Ross broilers, at 21 days of age, were randomly assigned to three treatments with eight cages per treatment. Three levels of $1{\alpha}$-OH $D_3$ (0, 5, and 10 ${\mu}g$/kg) were added to a basal diet (0.50% Ca, 0.13% non-phytate phosphorus (NPP), and 0.35% total phosphorus (tP)) without vitamin $D_3$. As a result of this study, the addition of $1{\alpha}$-OH $D_3$ increased body weight gain (p<0.001), feed intake (p = 0.007), feed efficiency (p<0.001), tibia weight (p = 0.002), length (p<0.001), breaking-strength (p = 0.012), ash (p<0.001), Ca (p<0.001), and P content (p = 0.004). Dietary $1{\alpha}$-OH $D_3$ enhanced breast meat yellowness (p = 0.015) and the length and weight of the small intestine of the broilers. Moreover, $1{\alpha}$-OH $D_3$ decreased serum Ca concentration (p = 0.074) and breast meat redness (p = 0.010). These results indicate that the $1{\alpha}$-OH $D_3$ improves growth, tibia quality, and meat color in broilers fed Ca- and P-deficient corn-soybean meal diets.

• Asian-Australasian Journal of Animal Sciences
• /
• 제29권12호
• /
• pp.1761-1767
• /
• 2016

Two experiments were conducted to evaluate effects of coated compound proteases (CC protease) on apparent total tract digestibility (ATTD) of nitrogen (N) and energy, and apparent ileal digestibility (AID) of amino acids (AA) and nutrients in diets for pigs. In Exp. 1, 12 crossbred barrows (initial body weight: $20.79{\pm}1.94kg$) were housed in individual metabolism crates and allotted into 2 treatments with 6 piglets per treatment according to weight in a randomized complete block design. The 2 diets were corn-soybean meal basal diets with (0.2 g/kg) or without CC protease supplementation. The CC protease supplementation increased (p<0.05) the digestible and metabolizable N and energy values and the digestibility and retention rate of N in the diet. The ATTD of energy and nutrients had been improved (p<0.05) in the diet supplemented with CC protease. In Exp. 2, 12 crossbred barrows (initial body weight: $20.79{\pm}1.94kg$), fitted with T-cannulas at the distal ileum, were blocked by body weight into 2 groups with 6 pigs each. The diets were the same as those in Exp. 1. The CC protease increased (p<0.05) the AID of crude protein and some essential AA including arginine, isoleucine and leucine. The AID and ATTD of energy and nutrients had been improved (p<0.05) by supplemental CC protease, but the hindgut digestibility of nutrients was unaffected. Overall, the CC protease improved the ATTD of N and energy and AID of some indispensible AA and nutrients in the corn-soybean meal diet for pigs. Therefore, the CC protease supplement could improve the utilization of protein in the corn-soybean meal diet and thus contribute to lower N excretion to the environment.

• Journal of Preventive Medicine and Public Health
• /
• 제22권1호
• /
• pp.153-161
• /
• 1989

Authors studied on the prevalence of 94 cases of pneumoconiosis who were found out through the screening test on 1,062 workers engaged in welding process at 36 shipyard in Pusan area from March 1st, 1986 to November 30th, 1986. The result were as follows; 1. Dust concentration was measured $4.49{\pm}0.54mg/m^3$ in the small scale shipyard while it was $6.25{\pm}1.08mg/m^3$ in the large one. 2. The prevalence of welder's lung was 8.9% (male:8.5%, female:12.0%) and this is consist of 4.9% suspected pneumoconiosis and 4.0% pneumoconiosis more than category 1/0. 3. The prevalence was significantly increased according to the duration of dust exposure(p<0.05), and it showed the increasing tendency by the age group. 4. The prevalence was significantly higher in the large scale shipyard than is the small scale one(p<0.01). 5. The type and shape of opacities were 71.4% of p type and 28.6% of q type, however no pneumoconiosis with r type observed in this study. 6. The main subjective symptoms were the sputum(29.8%), coughing(25.5%), shortness of breath(20.2%), fatique(6.4%), and chest pain(5.3%). In other hand, 57.4% of pneumoconiosis were asymptomatic. 7. The prevalence of pulmonary tuberculosis was radiologically 1.7% in all subjects including 5.3% in pneumoconiosis and 1.3% in no pneumoconiosis(p<0.01).

• Biomolecules & Therapeutics
• /
• 제32권1호
• /
• pp.154-161
• /
• 2024

Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disorder that causes progressive paralysis. L-Citrulline is a nonessential neutral amino acid produced by L-arginine via nitric oxide synthase (NOS). According to previous studies, the pathogenesis of ALS entails glutamate toxicity, oxidative stress, protein misfolding, and neurofilament disruption. In addition, L-citrulline prevents neuronal cell death in brain ischemia; therefore, we investigated the change in the transport of L-citrulline under various pathological conditions in a cell line model of ALS. We examined the uptake of [¹⁴C]L-citrulline in wild-type (hSOD1wt/WT) and mutant NSC-34/ SOD1^G93A (MT) cell lines. The cell viability was determined via MTT assay. A transport study was performed to determine the uptake of [¹⁴C]L-citrulline. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis was performed to determine the expression levels of rat large neutral amino acid transported 1 (rLAT1) in ALS cell lines. Nitric oxide (NO) assay was performed using Griess reagent. L-Citrulline had a restorative effect on glutamate induced cell death, and increased [¹⁴C]L-citrulline uptake and mRNA levels of the large neutral amino acid transporter (LAT1) in the glutamate-treated ALS disease model (MT). NO levels increased significantly when MT cells were pretreated with glutamate for 24 h and restored by co-treatment with L-citrulline. Co-treatment of MT cells with L-arginine, an NO donor, increased NO levels. NSC-34 cells exposed to high glucose conditions showed a significant increase in [¹⁴C]L-citrulline uptake and LAT1 mRNA expression levels, which were restored to normal levels upon co-treatment with unlabeled L-citrulline. In contrast, exposure of the MT cell line to tumor necrosis factor alpha, lipopolysaccharides, and hypertonic condition decreased the uptake significantly which was restored to the normal level by co-treating with unlabeled L-citrulline. L-Citrulline can restore NO levels and cellular uptake in ALS-affected cells with glutamate cytotoxicity, pro-inflammatory cytokines, or other pathological states, suggesting that L-citrulline supplementation in ALS may play a key role in providing neuroprotection.

• Asian-Australasian Journal of Animal Sciences
• /
• 제18권10호
• /
• pp.1485-1491
• /
• 2005

This experiment was conducted to investigate the effects of iron from an amino acid complex (Availa-$Fe^{\circledR}$) on the iron status of neonatal and suckling piglets. A total of 24 gestating sows (Landrace${\times}$Large White) were randomly allocated to three dietary treatments. The control diet contained 80 mg $kg^{-1}$ Fe from ferrous sulfate heptahydrate ($FeSO_4$.$7H_2O$), while the two experimental diets were supplemented with an additional 120 mg $kg^{-1}$ Fe from Availa-$Fe^{(R)}$ or $FeSO_4$.$7H_2O$, respectively. The lactating sows remained the same iron treatments as gestating sows, while neonatal piglets of 24 litters born from the above sows were allotted to another three treatments. Piglets from the sows of the control treatment were fed basal diet with no supplemental Fe as control treatment, but were injected with 100 mg Fe as Fe dextran at birth. Piglets from the sows of Availa-$Fe^{(R)}$ or $FeSO_4$.$7H_2O$ treatments were supplemented with 120 mg $kg^{-1}$ iron from Availa-$Fe^{(R)}$ or $FeSO_4$.$7H_2O$, respectively. The total born alive and weaned, and the average piglets weight at birth and at weaning were not significantly affected by the sow' dietary treatments (p>0.05). Iron from Availa-$Fe^{(R)}$ did not demonstrate a statistically significant improvement in hemoglobin concentration, hematocrit and plasma iron of sows on day 90 and 105 of pregnancy and the milk iron of sows during lactation (p>0.05). Neonatal piglets in the Availa-$Fe^{(R)}$ treatment had a significantly higher hemoglobin concentration (p<0.05) and higher hematocrit and plasma iron (p>0.05) than those in the other two treatments, respectively. The hemoglobin of suckling piglets in the Availa-$Fe^{(R)}$ treatment was higher than that of piglets in $FeSO_4$.$7H_2O$ treatment on day 28 (p<0.05). The total iron binding capacity of piglets in Availa-$Fe^{(R)}$ treatment was lower than that of piglets in the control and $FeSO_4$.$7H_2O$ treatment on day 14 (p<0.05), but there was not a statistically significant difference among three treatments on day 28 (p>0.05). However, the hemoglobin and hematocrit of suckling piglets injected with Fe were higher than those of piglets in the other two treatments (p<0.05). This study indicated that the addition of 120 mg $kg^{-1}$ iron from amino acid complex into the diets improved iron status of neonatal and nursing piglets more effectively than the addition of 120 mg $kg^{-1}$ iron from $FeSO_4$.$7H_2O$, however, this improvement of the organic Fe was not sufficient to replace the Fe injection for prevention of iron-deficiency anemia.

• 한국자원식물학회지
• /
• 제13권3호
• /
• pp.227-242
• /
• 2000

음나무 자생지의 보존과 복원을 위한 연구의 일환으로 개체목 단위로 산재하여 서식하는 것으로 알려진 음나무의 군집을 탐색하고, 인위적 간섭이 적고 보전상태가 양호한 강원도 흥정산, 발왕산, 가리왕산 지역을 대상으로 지형, 토양특성 등의 물리적 환경과 식생구조를 조사하였다. 1. 음나무 자생지의 입지환경 조사 결과 분포범위는 해발고 780~1,300m의 북서, 북동 및 북향을 중심으로 경사가 심한 산복-사면에 위치하였으며, 토양 산도는 5.81($\pm$0.05)로 비교적 중성에 가까운 토양이며, 양이온치환용량(C.E.C.)은 평균 22.30($\pm$8.1)(me/100g), 유기물함량 8.63($\pm$0.48)%로 비옥한 토양이였다. 2. 음나무의 평균 연륜 생장량은 1.60~2.41mm/year으로 발왕산이 2.41mm/year로 가장 높고, 가리왕산 1.98mm/year, 흥정산 1.60mm/year 순으로 생장량을 나타냈다. 초기 20년간의 생장량은 평균 생장량 보다 컸으나, 그 후는 계속적으로 감소하는 경향을 나타냈다. 3. TWINSPAN에 의한 군집의 분리는 지역간의 군집분리에 중요하게 작용하였으며, 음나무림의 평균 상대우점치 (MIV)는 흥정산지역 24.55%, 가리왕산지역 29.25% 그리고 발왕산지역 38.28%로 나타났으며, 종다양도는 전체적으로 1.3124~1.3992 범위를 보여 대체적으로 3개 지역간에 큰 차이는 보이지 않았다. 4. 음나무 층위별 분포형(Morista's index)은 교목층, 아교목층 및 관목층이 각각 0.9629, 2.9570, 20.5419로 나타나 교목층은 임의 분포하는 경향을 보였으나 아교목층과 관목층은 집중분포하는 것으로 나타났다. 5. 종다양성 분석 결과 상대우점도의 범위가 0.1476~0.2065으로 몇몇 종에 의한 단순림을 이루지 않고 다수의 종에 의해 혼효되어 자생하고 있으며, 유사도지수는 64.31~64.54% 범위로 3개 조사지역간에 식생의 연속성이 있는 것으로 판단되었다. 6. 조사지의 상대우점치를 이용한 종간 상관관계를 조사한 결과 음나무는 사스레나무, 물참대, 까치박달나무 그리고 들메나무와는 동일한 생태적 지위를 갖는 것으로 나타났으며, 노린재나무 1%, 쪽동백, 고로쇠 나무, 나래회나무, 느릅나무는 5%수준의 부의 상관관계를 보여 생태적 지위가 다른 것으로 나타났다. 7. 직경급 분포에 의한 개체군의 생육 동태를 예측한 결과 흥정산 지역은 정규분포를 보여 앞으로 지속적인 유지가 가능하며, 발왕산의 경우에도 소경목에서 대경목에 이르기까지 골고루 출현하여 음나무림의 지속적인 유지가 가능할 것으로 생각되나 가리왕산의 경우는 치수나 어린 개체가 출현이 되지 않고 중경급과 높은 직경급의 개체의 밀도도 높지 않아 앞으로 이 집단에서는 음나무 집단의 지속적인 유지가 어려울 것으로 생각된다.