• Title/Summary/Keyword: G.H.G

Search Result 25,195, Processing Time 0.054 seconds

Physicochemical Properties and Protective Effects of Corni fructus Treated with Pressurized-Steam against H2O2-Induced Cytotoxicity on L132 Cells (가압증숙공정에 의한 산수유의 이화학적 특성 및 과산화수소에 의해 유도된 산화적 L132 세포 사멸에 대한 보호 효과)

  • Park, Hye-Mi;Hong, Joo-Heon
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.46 no.9
    • /
    • pp.1061-1070
    • /
    • 2017
  • This study examined the physicochemical properties and protective effects of Corni fructus treated with pressurized-steam (through $121^{\circ}C$, $1.2kgf/cm^2$, 0.5 h, 1 h, 2 h, and 3 h) against $H_2O_2$-induced cytotoxicity on L132 cells. The color values of the untreated Corni fructus powder were higher than those of Corni fructus after the pressurized-steam treatment (PSC), and those of PSC improved with a decrease in treatment time. At the observation by pressurized-steam treatment for more than 2 h, the color was changed to black, and its gloss was lost. The major constituents in PSC (2 hours) were the total sugar (468.53 mg/g), reducing sugar (385.55 mg/g), and total phenol (37.32 mg/g), respectively. The main components in the free sugars of PSC (2 h) were fructose, glucose, and sucrose, at 207.72 mg/g, 219.40 mg/g, and 4.31 mg/g, respectively. The gallic acid in the phenol compounds and 5-(hydroxymethyl) furfural in the furan compounds of PSC (2 h) improved with increasing treatment time. The main components in iridoid glycoside of PSC (2 h) were morroniside, loganin, and lognic acid, which improved with decreasing treatment time. The L132 cell growth inhibition activities of all the extracts were significantly higher than that of the control. The protective effects against the $H_2O_2$-induced cytotoxicity on L132 cells of PSC (2 h) was 102.82% (at $1,000{\mu}g/mL$) higher than those of the other extracts. This suggests that Corni fructus by PSC is useful for functional food materials in the food industry.

Sensitivity of Escherichia coli to Seaweed (Ascophyllum nodosum) Phlorotannins and Terrestrial Tannins

  • Wang, Yuxi;Xu, Z.;Bach, S.J.;McAllister, T.A.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.22 no.2
    • /
    • pp.238-245
    • /
    • 2009
  • Pure culture experiments were conducted to assess the bacteriostatic and bactericidal effects of phlorotannins (PT) isolated from Ascophyllum nodosum (brown seaweed) on Escherichia coli O157:H7. In Exp. 1, one non-O157:H7 strain (25922) and three strains of E. coli O157:H7 (3081, EDL933 and E318N) were cultured in M9 medium with PT included at 0 (control), 25, 50 or $100{\mu}g/ml$ (n = 3). Bacterial growth was monitored by $OD_{600}$ at 0, 4, 6, 12 and 24 h, and by dilution plating at 0, 4, 6 and 24 h. All strains were inhibited (p<0.001) by PT to varying degrees. At 50 or $100{\mu}g/ml$, PT prevented growth of all four strains. At $25{\mu}g\;PT/ml$, growth of 25922, 3081, E318N and EDL933 was inhibited for 6, 12 and 24 h, respectively, but 25922 and 3081 resumed growth by 12 and 24 h. Direct plating confirmed bactericidal effects of PT on all four strains at $100{\mu}g/ml$, and on EDL933 and E318N at $50{\mu}g/ml$. In Exp. 2, strains 25922 and 3081 were incubated with no tannins or with $50{\mu}g/ml$ of PT, purified condensed tannins (CT) from Quebracho (Schinopsis balansaei), or purified tannic acid from Rhus semialata (Anacardiaceae) as hydrolysable tannins (HT). Strain 3081 was unaffected by HT or CT, but was completely inhibited (p<0.001) by PT at 4, 6 and 24 h. Strain 25922 was unaffected by HT, slightly inhibited by CT, and almost eradicated by PT at 4 and 6 h. Transmission electron microscopy revealed tannin-mediated alterations to bacterial cell walls. Phlorotannins from A. nodosum exhibit growth-inhibiting and bactericidal effects in vitro against the strains of E. coli O157:H7 investigated. Anti-E. coli efficacy of A. nodosum PT is superior to that of terrestrial tannins purified from Quebracho and from Rhus semialata.

High Density Cell Culture of Bifidobacterium by Optimization of Medium Composition and Culture Conditions. (배지조성 및 배양환경 최적화에 의한 Bifidobacterium의 고농도 배양)

  • 송수한;김택범;지근억;오훈일;오덕근
    • Microbiology and Biotechnology Letters
    • /
    • v.30 no.1
    • /
    • pp.63-67
    • /
    • 2002
  • Bifidobacterium strain was isolated from the feces of brast fed infants. The isolated strain was identified as Bifidobacterium longum by 16S rRNA sequence analysis and named as Bifidobacterium SH2. The MRS medium was modified to obtain high density cells of Bifidobacterium SH2. The optimal medium was determined to be 50 g/L lactose, 10 g/L beef extract, 10 g/L peptone, 5 g/L yeast extract, 7 g/L sodium acetate, 2 g/L ammonium citrate, 2 g/L disodium phosphate,1 g/L tween 80, 0.2 g/L MnSO$_4$ and 0.5 g/L L-cysteine. The pH and temperature were optimized as 5.0 and $37^{\circ}C$, respectively. Through out the optimization of medium composition and culture conditions, the dry cell weight and viable cell count were 2.5 times and 1.8 times higer than those in MRS medium, respectively.

Benzene Biodegradation Using the Polyurethane Biofilter Immobilized with Stenotrophomonas maltophilia T3-c

  • Kwon, Heock-Hoi;Lee, Eun-Young;Cho, Kyung-Suk;Ryu, Hee-Wook
    • Journal of Microbiology and Biotechnology
    • /
    • v.13 no.1
    • /
    • pp.70-76
    • /
    • 2003
  • The benzene removal characteritics of the polyurethane (PU) biofilter immobilized with S. maltophilia T3-c, that could efficiently degrade benzene, was investigated. Maximum capacity to eliminate benzene was maintained at $100-110g{\cdot}m^-3{\cdot}h^-1$ when space velocity (SV) ranged from 100 to $300 h^-1$ -1/, however, it decreased sharply to $55 g{\cdot}m^-3{\cdot}h^-1^$ as SV increased to $400 h^-1$. The critical elimination capacities that guaranteed $90\%$ removal of inlet loading of the PU biofilter were determined to be 70,30, and $15 g{\cdot}m^-3{\cdot}h^-1$ at SV 100,200, and $300 h^-1$, respectively. Based on the result of a kinetic analysis of the PU biofilter, maximum benzene elimination velocity ($V_m$) was $125 g{\cdot}m^-3^\;of\;PU{\cdot}h^-1$ and saturation constant ($K_m$) was $0.22 g{\cdot}m^-3^$ of benzene ($65{\mu}{\cdot}I^-1$). This study suggests that the biofilter utilizing S. maltophilia T3-c and polyurethane is a very promising technology for effectively degrading benzene.

Surface Characteristics and Adsorption Capacity of $H_2$S on the Activated Carbon Impregnated with NaOH (NaOH로 첨착된 활성탄의 표면특성과 $H_2$S 흡착능)

  • 박병배;이석기;박영성
    • Journal of the Korean Ceramic Society
    • /
    • v.38 no.4
    • /
    • pp.319-324
    • /
    • 2001
  • 본 연구에서는 NaOH로 첨착시킨 활성탄의 표면특성변화와 H$_2$S 흡착능을 고찰하였다. 첨착시약으로 사용된 NaOH 용액의 농도는 1~8N이며, 활성탄의 입자크기는 8$\times$30mesh가 적용되었다. 실험결과는 첨착율이 0.87~5.8% 범위 내에서 증가할수록 BET 표면적은 1050$m^2$/g에서 783$m^2$/g로 감소하며, 표면산도는 0.541meq/g-AC에서 0meq/g-AC으로 감소하고, pH는 9.56에서 10.86으로 증가하는 것으로 밝혀졌다. 또한 NaOH로 첨착시킨 활성탄의 H$_2$S 평형흡착능을 보임으로써 비첨착활성탄에 비해 2~3배 높은 수준을 나타냄을 알 수 있었다.

  • PDF

Methods for Improving Hand-off of Different Generations in CDMA Mobile Communications

  • Choi, Dong-You;Park, Chang-Kyun
    • The Journal of the Acoustical Society of Korea
    • /
    • v.21 no.2E
    • /
    • pp.91-97
    • /
    • 2002
  • One of the most important things in mobile phone service is Hand-Off (H/O). Meantime soft H/O and softer H/O have been used between the same generations (2G↔2G) and there is little problem. With user's needs and the development of communication technology, the system with different generation coexists and pilot beacon and time-periodic beacon are used for H/O between generations (2.5G↔2G) to start service, but it is not economic and efficient. To improve such problems, ENHHO developed in April 2001 has recently used. Unfortunately, this method also has the defect of momentary cutoff of communication information during the procedure of H/O in case of more than 5 pilot signals. Accordingly, we suggest algorithm to improve the problem of ENHHO and examines its propriety by analyzing the results of field survey using the algorithm.

ABSTRACT RELATIVE FOURIER TRANSFORMS OVER CANONICAL HOMOGENEOUS SPACES OF SEMI-DIRECT PRODUCT GROUPS WITH ABELIAN NORMAL FACTOR

  • Farashahi, Arash Ghaani
    • Journal of the Korean Mathematical Society
    • /
    • v.54 no.1
    • /
    • pp.117-139
    • /
    • 2017
  • This paper presents a systematic study for theoretical aspects of a unified approach to the abstract relative Fourier transforms over canonical homogeneous spaces of semi-direct product groups with Abelian normal factor. Let H be a locally compact group, K be a locally compact Abelian (LCA) group, and ${\theta}:H{\rightarrow}Aut(K)$ be a continuous homomorphism. Let $G_{\theta}=H{\ltimes}_{\theta}K$ be the semi-direct product of H and K with respect to ${\theta}$ and $G_{\theta}/H$ be the canonical homogeneous space (left coset space) of $G_{\theta}$. We introduce the notions of relative dual homogeneous space and also abstract relative Fourier transform over $G_{\theta}/H$. Then we study theoretical properties of this approach.

The Effects of Condensed Molasses Solubles(CMS) / Molasses Mixtures on Ruminal Microbial Protein Synthesis (Condensed Molasses Solubles(CMS) / 당밀 혼합물이 반추위 미생물 단백질 합성량에 미치는 영향)

  • Yeo, J.M.;Jeong, S.G.;Kim, H.S.;Ahn, B.S.;Kim, C.H.;Shin, H.T.
    • Journal of Animal Science and Technology
    • /
    • v.46 no.1
    • /
    • pp.61-68
    • /
    • 2004
  • An experiment was conducted to evaluate condensed molasses solubles(CMS, a by-product from monosodium glutamate production) as a source of nitrogen for ruminant with particular reference to its effects on microbial protein synthesis. Four non-lactating dairy cows fitted with rumen cannulas were used in a 4 ${\times}$ 4 Latin square with 14-day periods. The four treatments were (1) basal diet consisting of barley straw ad libitum and 3 kg/d of rolled barley, (2) basal diet plus 200 gld molasses and 300 g/d water, (3) basal diet plus 200 g/d molasses, 100 g/d CMS and 200 g/d water, (4) basal diet plus 200 g/d molasses, 200 g/d CMS and 100 g/d water. Ruminal pH remained at high levels and showed little variation during the day between treatments. The concentration of total and individual VFA in the rumen was similar between treatments. There was no difference in the concentration of ammonia in the rumen between treatments, although the intake of nitrogen in molassesl CMS mixture treatments was higher than that of control and molasses treatment. But there was a suggestion of an increased synthesis of microbial protein with the higher level of inclusion of CMS when the allantoin/creatinine ratio was used as an index of microbial protein production(P <0.10).

Evaluation of Houttuynia cordata and Taraxacum officinale on Growth Performance, Nutrient Digestibility, Blood Characteristics, and Fecal Microbial Shedding in Diet for Weaning Pigs

  • Yan, L.;Zhang, Z.F.;Park, J.C.;Kim, I.H.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.25 no.10
    • /
    • pp.1439-1444
    • /
    • 2012
  • A total of 144 pigs ((Landrace${\times}$Yorkshire)${\times}$Duroc)] with an average initial BW of $8.45{\pm}0.57$ kg were used in a 5-wk growth trial. Pigs were randomly allocated to 4 treatments with 9 replications per pen in a randomized complex block design. Dietary treatments included: i) CON (basal diet), ii) ANT (CON+tylosin 1 g/kg), iii) H1 (CON+H. cordata 1 g/kg) and iv) T1 (CON+T. officinale 1 g/kg). In this study, pigs fed the ANT and T1 treatment had a higher (p<0.05) average daily gain (ADG) and gain:feed (G:F) ratio than those fed CON and H1 treatment. Dietary ANT and T1 treatment led to a higher energy digestibility than the CON group. No difference (p>0.05) was observed on the growth performance and apparent total tract digestibility with H1 supplementation compared with the CON treatment. The inclusion of ANT treatment led to a higher (p<0.05) lymphocyte concentration compared with the CON treatment. Dietary supplementation of herbs did not affect (p>0.05) the blood characteristics (white blood cell (WBC), red blood cell (RBC), IgG, lymphocyte). No difference was observed on (p<0.05) fecal microbial shedding (E. coli and lactobacillus) between ANT and CON groups. Treatments H1 and T1 reduced the fecal E. coli concentration compared with the CON treatment, whereas the fecal lactobacillus concentration was not affected by the herb supplementation (p>0.05). In conclusion, the inclusion of T. officinale (1 g/kg) increased growth performance, feed efficiency, energy digestibility similarly to the antibiotic treatment. Dietary supplementation of T. officinale and H. cordata (1 g/kg) reduced the fecal E. coli concentration in weaning pigs.

A Study on the Game Analysis Methods : Focusing on the Analysis of Game Components (게임 구성요소를 중심으로 한 게임 분석 방법에 대한 고찰)

  • Nam, Ki-Teok;Yoon, Hyung-Sup
    • Journal of Korea Game Society
    • /
    • v.15 no.5
    • /
    • pp.19-28
    • /
    • 2015
  • The game industry has been seeing rapid change brought on by the competitive environment globally. As a result, game design skill is being magnified as a key to success. Because of lack of game design skills, Korean game market is in crisis recently. The game analysis is necessary to enhance game design skills. However, criteria and definition of game analysis is not clear and it is difficult to use for game development. This paper will suggest H-G model which has two factors that are 'human role' and 'the scope of game analysis'. This model segments 9 types of game analysis method. This study finds the limitation of leading researches and tries to improve it mapping the major leading researches to H-G model. This paper would help to make the game analysis be integrated and systemic research as regards 9 types of H-G model.