• Title/Summary/Keyword: G.729A

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Determination of Fructooligosaccharides and Raffinose in Infant Formula by High Performance Liquid Chromatography with Evaporative Light Scattering Detector (HPLC-ELSC를 이용한 조제분유 중 fructooligosaccharides 및 raffinose 분석)

  • Shin, Man-Sub;Park, Jae-Woo;Cho, Mi-Ran;Song, Sung-Ok;Kim, Chun-Sun;Choi, Chun-Bae;Lee, Seoung-Won;Lee, Ki-Woong;Chang, Chi-Hoon;Kwak, Byung-Man
    • Korean Journal of Food Science and Technology
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    • v.38 no.6
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    • pp.725-729
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    • 2006
  • A method was developed for the determination of fructooligosaccharides and raffinose contents in infant formula. The samples were extracted and analyzed by liquid chromatography equipped with carbohydrate column and evaporative light scattering detector. The mobile phase used for the gradient mode was water-acetonitrile, at a flow rate of 1.0mL/min. The method showed a mean recovery of 95-99%, the relative standard deviation obtained in the precision study was 0.774-8.982%, the quantification and detection limits were 25-50mg/L.

Changes in Quality Characteristics of Traditional Kochujang during Fermentation (재래식 고추장 숙성 중 품질특성의 변화)

  • Kim, Young-Soo;Shin, Dong-Bin;Jeong, Moon-Cheol;Oh, Hoon-Il;Kang, Tong-Sam
    • Korean Journal of Food Science and Technology
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    • v.25 no.6
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    • pp.724-729
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    • 1993
  • Quality characteristics of 3 kinds of traditional kochujang were investigated during 6 months of fermentation in order to obtain information for the industrial production of traditional kochujarg. Tested kochujang included Sunchang kochujang prepared with glutinous rice, Boeun kochujang prepared with barley, and Sachun kochujang prepared with wheat. The reducing sugar contents of Sunchang, Boeun, and Sachun kochujang increased up to 30, 90, and 150 days of fermentation, respectively, and then decreased thereafter. Ethanol contents of traditional kochujang increased up to $0.4{\sim}1.2%\;at\;120{\sim}150$ days of fermentation and decreased thereafter. Amino nitrogen content of Sunchang kochujang showed a slight increase during fermentation, however, those of Boeun and Sachun kochujang showed a rapid increase up to 150 days of fermentation. Ammoniacal nitrogen content of 100 g kochujang increased up to $50{\sim}75mg$ at 60 days of fermentation and leveled off thereafter. Capsaicin and capsanthin contents of 100 g kochujang were $15{\sim}18mg\;and\;60{\sim}180mg$ before fermentation and decreased to $12{\sim}14mg$ and $50{\sim}120mg$ at 180 days of fermentation, respectively.

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Optimum Application Level of Dicamba Herbicide for Control of Calystegia japonica in Silage Corn Field (사일리지용 옥수수 포장에서 메꽃 (Calystegia, japonica) 방제를 위한 Dicamba 액제 적정 시용수준 구명)

  • Seo, S.;Chung, E.S.;Kim, J.G.;Kang, W.S.;Kim, W.H.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.19 no.3
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    • pp.259-264
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    • 1999
  • A field experiment was carried out to determine the optimum application level of dicamba herbicide for efficient control of Calystegia japonica in silage corn field. Six treatments of application levels (0 ; control, $0.75{\ell},\;1.0{\ell},\;1.25{\ell},\;1.5{\ell},\;and\;2.0{\ell}/ha$) were arranged at National Livestock Research Institute, RDA, Suwon in 1995. The hybrid silage corn was DK 729, and dicamba herbicide was applied at 5~6 leaf stage of corn. The growth of corn was poor in control plot, and the days for tasseling and silking were delayed 1~4 days when compared with the plots of herbicide application. The control efficiency of Calystegia japonica was excellent as 74.3~94.6% in the plots of dicamba application. A slight injury of herbicide was observed at early stage of corn when applied at $1.25{\ell}$ and $1.5{\ell}$ of dicamba per ha, and severe injury was found at $2.0{\ell}$ of dicamba. Forage dry matter(DM) yield, and length of ear in the plots of dicamba treatments were significantly higher than those of control. The DM yield of control(4,866kg/ha) was only about 30% of dicamba treatment plot (14,960~16,340kg/ha). However, there was no yield difference among dicamba application level. The percentage of ear to total DM yield was ranged from 33.6 to 39.4%. With application of herbicide, the contents of crude protein and nitrogen free extract were increased, and that of crude fiber was decreased in stover. However, nutritive value of corn ear was very similar among all treatments, regardless of herbicide treatment. From the above results, it is recommended that optimum application level of dicamba herbicide was $0.75{\sim}1.0{\ell}/ha$ for efficient control of Calystegia japonica and for silage corn production without herbicide injury.

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Audio Stream Delivery Using AMR(Adaptive Multi-Rate) Coder with Forward Error Correction in the Internet (인터넷 환경에서 FEC 기능이 추가된 AMR음성 부호화기를 이용한 오디오 스트림 전송)

  • 김은중;이인성
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.26 no.12A
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    • pp.2027-2035
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    • 2001
  • In this paper, we present an audio stream delivery using the AMR (Adaptive Multi-Rate) coder that was adopted by ETSI and 3GPP as a standard vocoder for next generation IMT-2000 service in which includes combined sender (FEC) and receiver reconstruction technique in the Internet. By use of the media-specific FEC scheme, the possibility to recover lost packets can be much increased due to the addition of repair data to a main data stream, by which the contents of lost packets can be recovered. The AMR codec is based on the code-excited linear predictive (CELP) coding model. So we use a frame erasure concealment for CELP-based coders. The proposed scheme is evaluated with ITU-T G.729 (CS-ACELP) coder and AMR - 12.2 kbit/s through the SNR (Signal to Noise Ratio) and the MOS (Mean Opinion Score) test. The proposed scheme provides 1.1 higher in Mean Opinion Score value and 5.61 dB higher than AMR - 12.2 kbit/s in terms of SNR in 10% packet loss, and maintains the communicab1e quality speech at frame erasure rates lop to 20%.

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Packet Loss Concealment Algorithm Based on Speech Characteristics (음성신호의 특성을 고려한 패킷 손실 은닉 알고리즘)

  • Yoon Sung-Wan;Kang Hong-Goo;Youn Dae-Hee
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.31 no.7C
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    • pp.691-699
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    • 2006
  • Despite of the in-depth effort to cantrol the variability in IP networks, quality of service (QoS) is still not guaranteed in the IP networks. Thus, it is necessary to deal with the audible artifacts caused by packet lasses. To overcame the packet loss problem, most speech coding standard have their own embedded packet loss concealment (PLC) algorithms which adapt extrapolation methods utilizing the dependency on adjacent frames. Since many low bit rate CELP coders use predictive schemes for increasing coding efficiency, however, error propagation occurs even if single packet is lost. In this paper, we propose an efficient PLC algorithm with consideration about the speech characteristics of lost frames. To design an efficient PLC algorithm, we perform several experiments on investigating the error propagation effect of lost frames of a predictive coder. And then, we summarize the impact of packet loss to the speech characteristics and analyze the importance of the encoded parameters depending on each speech classes. From the result of the experiments, we propose a new PLC algorithm that mainly focuses on reducing the error propagation time. Experimental results show that the performance is much higher than conventional extrapolation methods over various frame erasure rate (FER) conditions. Especially the difference is remarkable in high FER condition.

Analysis of Amygdalin of Content Prunus mume by Variety, Harvest Time, and Fermentation Conditions (품종과 수확시기 및 발효조건에 따른 매실의 아미그달린 함량에 관한 연구)

  • Son, Seok Jun;Jeong, Young Jae;Kim, Sun Young;Choi, Ji Hae;Kim, Na Young;Lee, Hyun-Sun;Bae, Jung Min;Kim, Seon-Il;Lee, Hye-Suk;Shin, Jong Sup;Han, Jin Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.46 no.6
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    • pp.721-729
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    • 2017
  • This study aimed to improve customer perception of Prunus mume through analysis of amygdalin contents according to changes in variety, harvest time, and fermentation conditions. Five Prunus mume domestic cultivars were harvested at five harvest times. We compared cyanogenic glucosides in four types of fruits on the market. For amygdalin contents in seeds and flesh of Prunus mume by variety and harvest time, seeds contained higher amygdalin contents than flesh with time. As Prunus mume ripened, both amygdalin contents in seed and flesh increased. However, the rate of increase gradually decreased. For prunasin contents in Prunus mume, we determined that the dramatic increase in amygdalin from May 3 to 19 was due to amygdalin synthesis from prunasin. Moreover, in the case of fermented Prunus mume, we observed lower amygdalin content as the sugar ratio and fermentation time increased until around 90 days, followed by a decrease. Furthermore, we analyzed alteration of organic acids in Prunus mume and fermented solution based on analysis of amygdalin content in four other market fruits. Amygdalin was detected at $252.37{\pm}2.3$, $22.01{\pm}0.31$, and $8.75{\pm}0.14mg$ per 100 g in plums, peaches, and grape seeds, respectively. In flesh of plums and peaches, amygdalin contents were detected at $84.14{\pm}0.26$ and $7.54{\pm}0.06mg$ per 100 g, respectively. These results suggest scientific improvements for consumption and breeding lines.

Serum Biomarkers for Early Detection of Hepatocellular Carcinoma Associated with HCV Infection in Egyptian Patients

  • Zekri, Abdel-Rahman;Youssef, Amira Salah El-Din;Bakr, Yasser Mabrouk;Gabr, Reham Mohamed;El-Rouby, Mahmoud Nour El-Din;Hammad, Ibtisam;Ahmed, Entsar Abd El-Monaem;Marzouk, Hanan Abd El-Haleem;Nabil, Mohammed Mahmoud;Hamed, Hanan Abd El-Hafez;Aly, Yasser Hamada Ahmed;Zachariah, Khaled S.;Esmat, Gamal
    • Asian Pacific Journal of Cancer Prevention
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    • v.16 no.3
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    • pp.1281-1287
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    • 2015
  • Background: Early detection of hepatocellular carcinoma using serological markers with better sensitivity and specificity than alpha fetoprotein (AFP) is needed. Aims: The aim of this study was to evaluate the diagnostic value of serum sICAM-1, ${\beta}$-catenin, IL-8, proteasome and sTNFR-II in early detection of HCC. Materials and Methods: Serum levels of IL-8, sICAM-1, sTNFR-II, proteasome and ${\beta}$-catenin were measured by ELISA assay in 479 serum samples from 192 patients with HCC, 96 patients with liver cirrhosis (LC), 96 patients with chronic hepatitis C (CHC) and 95 healthy controls. Results: Serum levels of proteasome, sICAM-1, ${\beta}$-catenin and ${\alpha}FP$ were significantly elevated in HCC group compared to other groups (P-value<0.001), where serum level of IL-8 was significantly elevated in the LC and HCC groups compared to CHC and control groups (P-value <0.001), while no significant difference was noticed in patients with HCC and LC (P-value=0.09). Serum level of sTNFR-II was significantly elevated in patients with LC compared to HCC, CHC and control groups (P-value <0.001); also it was significantly higher in HCC compared to CHC and control groups (P-value <0.001). ROC curve analysis of the studied markers between HCC and other groups revealed that the serum level of proteasome had sensitivity of 75.9% and specificity of 73.4% at a cut-off value of $0.32{\mu}g/ml$ with AUC 0.803 sICAM-1 at cut off value of 778ng/ml, the sensitivity was 75.8% and the specificity was 71.8% with AUC 0.776. ${\beta}$-catenin had sensitivity and specificity of 70% and 68.6% respectively at a cut off value of 8.75ng/ml with an AUC of 0.729. sTNFR-II showed sensitivity of 86.3% and specificity of 51.8% at a cut off value of 6239.5pg/ml with an AUC of 0.722. IL-8 had sensitivity of 70.4% and specificity of 52.3% at a cut off value of 51.5pg/ml with AUC 0.631. Conclusions: Our data supported the role of proteasome, sICAM-1, sTNFR-II and ${\beta}$-catenin in early detection of HCC. Also, using this panel of serological markers in combination with ${\alpha}FP$ may offer improved diagnostic performance over ${\alpha}FP$ alone in the early detection of HCC.

The Difference in Chemokine Expression in Airway Epithelial Cells According to the Virulence of Tubercle Bacilli (결핵균 독성 여부에 따른 기도 상피세포의 Chemokine 발현에 관한 연구)

  • Kwon, O-Jung;Kim, Ho-Joong;Kim, Jung-Hee;Kim, Ho-Cheol;Suh, Gee-Young;Park, Jeong-Woong;Park, Sang-Joon;Chung, Man-Pyo;Choi, Dong-Chull;Rhee, Chong-H.
    • Tuberculosis and Respiratory Diseases
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    • v.44 no.4
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    • pp.729-741
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    • 1997
  • Background : We have recently reported that airway epithelial cells can produce RANTES and IL-8 in response to the stimulation of tubercle bacilli suggesting a certain role of airway epithelial cells in the pathogenesis of pulmonary tuberculosis. The pathogenesis of tuberculosis is determined by several factors including phagocytosis, immunological response of host, and virulence of tubercle bacilli. Interestingly, there have been reports suggesting that difference in immunological response of host according to the virulence of tubercle bacilli may be related with the pathogenesis of tuberculosis. We, therefore, studied the expressions and productions of RANTES and IL-8 in airway epithelial cells in response to tubercle bacilli(H37Rv, virulent strain and H37Ra, avirulent strain), in order to elucidate the possible pathophysiology of pulmonary tuberculosis. Methods : Peripheral blood monocytes were isolated from normal volunteers. Peripheral blood monocytes (PBM) were stimulated with LPS($10{\mu}g/ml$), H37Rv, or H37Ra($5{\times}10^5$ bacilli/well) along with normal control for 24 hours. A549 cells were stimulated with supernatants of cultured PBM for 24 hours. ELISA kit was used for the measurement of $TNF{\alpha}$ and IL-$1{\beta}$ production in supernatants of cultured PBM and for the measurement of RANTES and IL-8 in supernatants of cultured A549 cells. Northern blot analysis was used for the measurement of RANTES and IL-8 mRNA expression in cultured A549 cells. Results : $TNF{\alpha}$ and IL-$1{\beta}$ productions were increased in cultured PBM stimulated with LPS or tubercle bacilli(H37Rv or H37Ra) compared with the control. There was, however, no difference in $TNF{\alpha}$ and IL-$1{\beta}$ production between cultured PBM stimulated with H37Rv and H37Ra. RANTES and IL-8 expressions and productions were also increased in cultured A549 cells stimulated with LPS or tubercle bacilli compared with the control. RANTES and IL-8 mRNA expressions were significantly increased in cultured A549 cells stimulated with H37Ra-conditioned media(CM) compared with A549 cells stimulated with H37Rv-CM (p<0.05). However, there was no difference in RANTES and IL-8 productions between A549 cells stimulated with H37Rv-CM and H37Ra-CM. Conclusion : Airway epithelial cells can produce the potent chemokines such as RANTES and IL-8, in response to the stimulation of tubercle bacilli. These results suggest that airway epithelial cells may play a certain role in the pathogenesis of pulmonary tuberculosis. However, the role of airway epithelial cells in the pathogenesis of tuberculosis according to the virulence of tubercle bacilli was not clear in this study.

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