• Mycobiology
• /
• 제29권1호
• /
• pp.1-6
• /
• 2001

Nine species of genus Ganoderma collected in Korea and abroad including Ganoderma lucidum complex and G. lucidum were compared by investigating growth characteristics. In the bottle culture, the mycelial growth periods of G. lucidum from Taiwan and North America was 26 to 30 days compared to that of Korean G. lucidum, which was 30 to 32 days. Cultivation period of Taiwan and North American isolates was 30 to 32 days which were 11 to 17 days shorter than those of Korean isolates. Biological efficiency of Taiwan and North American isolates were ranged from 3.3 to 5.5%, which were apparently lower than that of Korean isolates which ranged from 6.2 to 9.4%. Korean isolates had longer stipes($15{\sim}40$ mm) and more number of pileus($4{\sim}6$/bottle) than those of Taiwan and North American isolates. The G. lucidum isolates collected from Korea will be regarded as the independent species from the G. lucidum collected from Taiwan and North America since, the G. lucidum from Korea showed much different growth characteristics in various aspects compared to the G. lucidum from Taiwan and North America.

• 한국식품영양학회지
• /
• 제7권1호
• /
• pp.45-50
• /
• 1994

This study was carried out to Investigate the effects of Panax ginseng, Ganoderma lucidum extract and crude polysaccharide of G. lucidum on the growth of lactic acid bacteria. p. ginseng extract contained 60.7% carbohydrate and 27.5% protein, whereas G. lucidum contained 35.9% carbohydrate and 46.3% protein. The total sugar and protein content of crude polysaccharide of G. lucidum were 47.2% and 15.2%, respectively. Two amino acids(hg, Trp) were detected in p. ginseng extract and 11 amino acids (hg, Trp, Ua, Lys, Ser, etc.) in C. lucidum extract. By the addition of p. ginseng, 5. lucidum extract and crude polysaccharide, the cia. p. ginseng was more effective on the growth of 1. casei an: G. lucidum was more effective on that of S. thermophilus. The effect of free amino acids on the growth of tactic acid bacteria was also examined. Arginine and lysine stimulated the growth of L. casei, whereas Lysine, serine, arginine, and glutamic acid stimulated the growth of 5. thermophilus.

• Archives of Pharmacal Research
• /
• 제19권5호
• /
• pp.423-428
• /
• 1996

Optimum conditions for hydrolysis were investigated to enhance water-solubilities of protein-bound polysaccharides in the basidiocarps of Ganoderma lucidum by treating chymotrypsin. We also attempted with Ganoderma lucidum residue remaining after extracting hot water-soluble compoents in Ganoderma lucidum. After hydrolyzing under optimum conditions (20 ppm chymotrypsin, 2% Gampderma lucidum or 6% Ganoderma lucidum residue, at pH 10 and at $ 40^{\circ}C$), the amounts of total protein and carbohydrate of hydrolysate were measured. Michaelis constant, $K_{m}$, and maximum rate, $V_{max}$, calculated by Lineweaver-Buck plot for the hydrolysis of Ganoderma lucidum were 1.73% and 0.073%/min respectively and those for hydrolysis of Ganoderma lucidum residue were 2.40% and 0.033%/min respectively. The amount of polysaccharide isolated from Ganoderma lucidum (100 g) treated with chymotrypsin was only 3.07 g, but significantly increased amount (14.34 g) of polysaccharides was isolated from Ganoderma lucidum residue (100 g) treated with chymotrypsin. The protein-bound polysaccharide was isolated from the non-hydrolyzed and hydrolyzed sample and molecular weights of the polysaccharide were measured by Sepharose CL-48 gel filtration.

• Mycobiology
• /
• 제29권2호
• /
• pp.80-84
• /
• 2001

Nine species of genus Ganoderma different in cultural characteristics each other were separated by histo-anatomical differences. Differences among the species and geographic distribution of G. lucidum were also analyzed to provide the criteria for the classification of Korean cultivation or wild type strains. Korean cultivation and wild type strains were quiet different from other species as well as Taiwan and North American G. lucidum strains in histo-anatomical traits of Ganoderma. Pore color of Korean G. lucidum strains was less brown than those of Taiwan and North American strains. Shapes of pores were round or circular in Korean strains but ellipsoidal or angular in Taiwan or North American strains. Pore numbers of Korean strains were more than those($4{\sim}6/mm$) of Taiwan or North American strains. Hardness of the pileus of Korean strains was much more than that of Taiwan or North American strains. Such characteristics of Korean strains were different from those of other species of Ganoderma. Korean G. lucidum strains could be classified into the other group because they had many different traits in growth characteristics of fruiting bodies and histo-anatomical characteristics from those of Taiwan or North American G. lucidum and other species of G. lucidum complex.

• 한국균학회지
• /
• 제24권1호통권76호
• /
• pp.67-80
• /
• 1996

Ganoderma속균은 약효가 인정되어 많이 인공 재배되어 이용되고 있으나 본 속균에 대한 기초적인 연구가 미흡한 실정이므로 본 연구에서는 영지 속균을 채집하여 분리 동정하였으며, 또한 배양적 특성과 형태적인 특징을 조사하였다. 영지속균은 경기도와 강원도 11개 장소의 참나무, 아카시나무, 오리나무 등에서와 6재배농가에서 채집되었다. 대부분의 영지는 하나의 버섯이 그루터기에서 형성되나 오리나무에서는 다수가 형성되었다. 영지속균의 균사 생장적온은 G. applanatum, G.lucidum, G. neo-japonicum에서는 $28{\sim}30^{\circ}C$이었고, G. tsugae에서는 $26^{\circ}C$이었으나 $32^{\circ}C$에서는 천천히 생장하였다. Hamda배지를 pH 6.2로 조정하였을 때 균사생장이 가창 좋았다. 6종의 공시균주에 대한 균사의 형태적인 특정으로 G. applanatum, G. lucidum, G. neo-japonicum은 staghorn를 형성하였으나 G.oregonens, G. ualeosiacum은 포도형의 가지를 가진 staghorn균사를 형성하였다. Clamp connection은 G. applanatum, G. lucidum, G. oregonense, G. valeosiacum균의 균사에서 관찰되었으나 node형을 가진 G. neo-japonioum에서는 형성되지 않았다. 후막포자는 G. applanatum, G. neo-japonicum에서 형성되었으며, cuticular cell는 G. lucidum, G. neo-japonicum, G. oregonense, G. tsugae의 균사에서 형성되었다.

• Mycobiology
• /
• 제44권1호
• /
• pp.1-6
• /
• 2016

Ganoderma lucidum has a long history of use as a traditional medicine in Asian countries. However, the taxonomy of Ganoderma species remains controversial, since they were initially classified on the basis of their morphological characteristics. Recently, it was proposed that G. lucidum from China be renamed as G. sichuanense or G. lingzhi. In the present study, phylogenetic analysis using the internal transcribed spacer region rDNA sequences of the Ganoderma species indicated that all strains of the Korean 'G. lucidum' clustered into one group together with G. sichuanense and G. lingzhi from China. However, strains from Europe and North American, which were regarded as true G. lucidum, were positioned in a clearly different group. In addition, the average size of the basidiospores from the Korean cultivated Yeongji strains was similar to that of G. lingzhi. Based on these results, we propose that the Korean cultivated Yeongji strains of 'G. lucidum' should be renamed as G. lingzhi.

• 한국균학회지
• /
• 제22권4호
• /
• pp.286-297
• /
• 1994

Ganoderma lucidum이 생산하는 polygalacturonase의 유용 활용 방안을 위한 최적 생산조건을 검토한 결과는 다음과 같다. Polygalacturonase 생산을 위한 최적 배양조건은 $30^{\circ}C$, pH5.5에서 14일이었다. Polygalacturonase 생산을 위한 최적 영양조건은 pectin 10 g, soluble starch 10 g, yeast extract 1 g, peptone 2 g, phenylalanine 1 g, $KH_2PO_4$ 2 g, $MgSO_4$ 0.2 g, $CaCI_2$ 50 mg, $thiamin{\cdot}HCI\;100{\mu}g$, D.W. 1000ml이었다.

• 한국식품과학회지
• /
• 제36권4호
• /
• pp.655-661
• /
• 2004

Ganoderma lucidum 균사체의 액체 배양을 통하여 균체 내 sphingolipid를 분리 정제하여 그 구조를 규명하였으며, 피부보습을 비롯한 각종의 기능성을 탐색하여 화장품 소재 및 새로운 생물 산업소재의 이용가능성을 검토한 결과 배양한 Ganoderma lucidum로부터 일련의 과정을 통하여 crude sphingolipids를 얻고, methanol침전, Dowex AG $50W-X8(H^+\;form)$ cation exchange chromatography 및 preparative thin layer chromatography를 행하여 sphingolipids를 분리하였다. 생산량은 0.4g/L였으며, 수율은 1%였다. UV/VIS spectrum, FT-IR 및 1H-NMR 분석을 행하여 구조를 해석한 결과 phytosphingosine 유도체로 판명되었다. 이 phytosphingosine 유도체의 피부에 대한 보습효과는 $500{\mu}g/mL$ 이상의 농도에서 약 20% 정도 skinmate 값이 증가하였으며, $100{\mu}/mL$ 이상의 농도에서 거칠기 감소에 유의한 결과를 나타내었다. 이상의 결과로부터 Ganoderma lucidum이 생산하는 sphingolipids는 피부보습 및 거칠기 개선에 유의성이 확인되어 화장품 소재 및 새로운 생물 산업소재로서의 이용가능성이 높다고 할 수 있다.

• 산업기술연구
• /
• 제21권A호
• /
• pp.337-341
• /
• 2001

This study was carried out to screen the effective polymeric additives preventing wall growth during mycelial submerged cultivation of Ganoderma. lucidum. Effects of additives on mycelial growth and exo-polysaccharide (EPS) production in flask culture and jar fermenter system under 3 different pH processes were investigated, and changes of mycelial morphology were also examined. From flask culture of G. lucidum with additives of different concentrations, 0.1%(w/v) polyacrylic acid was effective for EPS production. As the polyacrylic acid of 0.1%(w/v) was added in medium, wall growth of G. lucidum mycelium grown in jar fermenter system could be protected. The addition of 0.1%(w/v) polyacrylic acid to medium was also improved the mycelial growth and EPS production in the later of submerged culture G. lucidum and no changes of mycelial morphology were observed.

• 한국균학회지
• /
• 제37권2호
• /
• pp.167-172
• /
• 2009

Mycelia of Pleurotus ostreatus, Phellinus linteus, Ganoderma lucidum and Lentinus edodes were cultured in the selected cereals to generate functionally active cereals. The optimum water contents for the mycelial growth were 50%(wt/wt) for brown rice, barley and soybean and 75% for wheat and corn, respectively. P. ostreatus grew well in the most cereals while the mycelial growth of P. linteus, G. lucidum and L. edodes in soybean were siginificantly retarded. The contents of β-glucan and glucosamine in the mycelial cereals were determined. Wheat cultured with mushroom mycelia showed high ß-glucan content. Especially, wheat with G. lucidum contained the highest value of 26.16%. Soybean cultured with G. lucidum showed two-fold increase in glucosamine content with 9.63% of total mass while wheat showed 7.91%. Overall, wheat cultured with G. lucidum was the best functional cereal in terms of β-glucan and glucosamine contents.