• Tuberculosis and Respiratory Diseases
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• v.42 no.2
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• pp.165-174
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• 1995

Background: Exercise is one of the most common precipitants of acute asthma encountered in clinical practice. The development of airflow limitation that occurs several minutes after vigorous exercise, i. g. exercise-induced bronchoconstriction(EIB), has been shown to be closely correlated with the nonspecific bronchial hyperresponsiveness, which is the hallmark of bronchial asthma. All previous reports that assessed the correlation of EIB to nonspecific bronchial hyperresponsiveness have focused on airway sensitivity($PC_{20}$) to inhaled bronchoconstrictor such as methacholine or histamine. However, maximal airway narrowing(MAN), reflecting the extent to which the airways can narrow, when being exposed to high dose of inhaled stimuli, has not been studied in relation to the degree of EIB. Methods: Fifty-six children with mild asthma(41 boys and 15 girls), aged 6 to 15 years(mean${\pm}$SD, $9.9{\pm}2.5$ years) completed this study. Subjects attended the laboratory on two consecutive days. Each subject performed the high-dose methacholine inhalation test at 4 p.m. on the first day. The dose-response curves were characterized by their position($PC_{20}$) and MAN, which was defined as maximal response plateau(MRP: when two or three data points of the highest concentrations fell within a 5% response range) or the last of the data points(when a plateau could not be measured). On the next day, exercise challenge, free running outdoors for ten minutes, was performed at 9 a.m.. $FEV_1$ was measured at graduated intervals, 3 to 10 minutes apart, until 60 minutes after exercise. Response(the maximal ${\triangle}FEV_1$ from the pre-exercise value) was classified arbitrarily into three groups; no response((-) EIB: ${\triangle}FEV_1$<10%), equivocal response ($({\pm})$EIB:10%<${\triangle}FEV_1$<20%) and definite response($({\pm})$EIB:${\triangle}FEV_1$>20%). Results: 1) When geometric mean $PC_{20}$ of the three groups were compared, $PC_{20}$ of (+) EIB group was significantly lower than that of (-)EIB group. 2) There was a close correlation between $PC_{20}$ and the severity of EIB in the whole group(r=-0.568, p<0.01). 3) Of the total 56 subjects, MRP could be measured in 36 subjects, and the MRP of these subjects correlated fairly with the severity of EIB(r=0.355, p<0.05) 4) The MAN of (+) EIB group was significantly higher than that of (-)EIB group(p<0.01). 5) The MAN correlated well with the severity of EIB in the whole group(r=0.546, p<0.01). Conclusion: The degree of MAN as well as bronchial sensitivity($PC_{20}$) to methacholine is correlated well with the severity of EIB. The results suggest that the two main components of airway hyperresponsiveness may be equally important determinants of exercise reactivity, although the mechanism may be different from each other. The present study also provides further evidence that EIB is a manifestation of the increased airway reactivity characteristic of bronchial asthma.

• Clinical and Experimental Pediatrics
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• v.53 no.1
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• pp.21-27
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• 2010

Purpose : In contrast with traditional time-cycled, pressure-limited ventilation, during volume-controlled ventilation, a nearly constant tidal volume is delivered with reducing volutrauma and the episodes of hypoxemia. The aim of this study was to compare the efficacy of pressure-regulated, volume controlled ventilation (PRVC) to Synchronized intermittent mandatory ventilation (SIMV) in VLBW infants with respiratory distress syndrome (RDS).Methods : 34 very low birth weight (VLBW) infants who had RDS were randomized to receive either PRVC or SIMV with surfactant administration : PRVC group (n=14) and SIMV group (n=20). We compared peak inspiratory pressure (PIP), duration of mechanical ventilation, and complications associated with ventilation, respectively with medical records. Results : There were no statistical differences in clinical characteristics between the groups. After surfactant administration, PIP was significantly lower during PRVC ventilation for 48hrs and accumulatevive value of decreased PIP was higher during PRVC ventilation for 24hrs (P<0.05). Duration of ventilation and incidence of complications was no significant difference. Conclusion : PRVC is the mode in which the smallest level of PIP required to deliver the preset tidal volume in VLBW infants with RDS, adaptively responding to compliance change in lung after surfactant replacement.

• Korean Journal of Pharmacognosy
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• v.40 no.3
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• pp.196-204
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• 2009

This study was carried out to investigate the in vivo and in vitro inhibitory effect of a traditional herbal complex (HC) extract prepared from a mixture of four oriental herbs (Dioscorea Rhizoma, Glycine soja Sieb. et Zucc, Bombycis corpus, Fermented Glycine soja) that have been widely used for the treatment and prevention of diabetes mellitus on hyperglycemia. The water extract of HC showed potent inhibitory effect on $\alpha$-glucosidase with $IC_{50}$ value of 1.24 mg/mL. Additionally, the ethanol extract of HC was also found to exhibit significant inhibitory effect against protein tyrosine phosphatase $1{\beta}$ ($PTP1{\beta}$), which is known as a major regulator of both insulin and leptin signaling. In the $PTP1{\beta}$ inhibitory assay, the most active n-hexane fraction obtained from the ethanol extract of HC, was identified as a mixture of fatty acid derivatives by gas chromatography-mass spectrometry (GC-MS). In high-fat diet-low dose streptozotocin (STZ)-induced diabetic rat, the water extract of HC improved the oral glucose intolerance as compared with rosiglitazone. HC also caused a marked decrease of body weight and fasting blood glucose and a significant improvement on glucose tolerance in metabolic syndrome mice model. These findings support that this traditional HC may be useful in the control of blood glucose in diabetes mellitus and metabolic syndrome.

• Korean Journal of Poultry Science
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• v.32 no.2
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• pp.125-133
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• 2005

Morphometric changes in testicular Sertoli and Leydig cells from hatching to adulthood were studied using Korean native chickens of 1, 2, 4, 6, 8, 10, 12, 14, 16, 18, 21, 24, 28, 32, 44, 52 and 64 weeks (n=13 chickens per group) of age. The objective of this study was to understand the developmental phase of the Sertoli and Leydig cells with age. Testis of chickens was fixed by whole body perfusion using a fixative containing $2.5\%$ glutaraldehyde in cacodylate buffer, processed and embedded in Epon-araldite. Using 1 Um sections stained with methylene blue-azure II, qualitative and quantitative (stereological) morphological studies were performed. The average volume of a testis of 1 week old Korean native chickens was determined as $0.148\;cm^3$ and the parameter increased linearly from 1 week to 21 weeks days $(28.86\;cm^3)$, and did not change from 21 weeks to 64 weeks. The volume density of the seminiferous tubules increased with age from $32.6\%$ at week 1 to $92.89\%$ at week 64. The volume density of the interstitium represents $67.4\%$ of the testicular parenchyma at week 1. This proportion progressively diminished during development to reach a value of $7.11\%$ at week 64. The volume density of the Leydig cells decreased almost linearly from 1 week $(4.9\%)$ to 14 weeks $(1.7\%)$ and remained unchanged thereafter. In contrast, the Sertoli cells occupied a volume density of $3.4\%$ at week 1, increased progressively up to 18 weeks of age $(10.79\%)$ and remained unchanged thereafter. The absolute volume of the Leydig and Sertoli cells per testis increased significantly from week 1 to week 21 but did not change significantly from week 24 to week 64. The number of Leydig cells per testis increased almost linearly from 1 week to 21 weeks, remained high and unchanged with advancing age. The number of Sertoli cells per testis increased gradually with age from 1 week to 14 weeks and remained unchanged thereafter.

• Asian Journal of Turfgrass Science
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• v.23 no.1
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• pp.1-8
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• 2009

This study was conducted to investigate the effect of rainfall, irrigation and fertilizer application on water qualities of pond in golf course from May in 2007 year to November in 2008 year. For survey of water quality, it was sampled at $10{\sim}12$ sites(irrigation site 2, drainage site 3, and hazard site $5{\sim}7$) of SKY72 G.C and evaluated with analysis items such as pH, electrical conductivity(EC), DO, SS, T-N, $PO_4$-P, K, Ca, Mg, Na, SAR, BOD and COD. Results obtained were summarized as follows: The value of pH, EC, DO, T-N, P04-P, K, Ca, Mg, Na and SAR was in dry season, but those of SS, BOD and COD in the rainy season. N and P concentration of pond water showed $1.2{\sim}28.8mg/L$ and $0.005{\sim}0.172mg/L$, respectively, and so it was higher than eutrophication level of lakes and marshes recommended by the Ministry of Environment in Korea. As a reault of correlation analysis, EC in the water was significantly(P<0.01) related items such as K, Ca, Mg, Na, SAR and salinity, and SS significantly(P<0.05) BOD and COD. In comparison with a corelation between respective analysis items and rainfall amount, SAR was significantly(P<0.01) positive but T-N negative(P<0.01). As compared with corelation between respective items and irrigation amount, pH was significantly(P<0.01) positive but T-N(P<0.05) and BOD(P<0.01) negative. When it was applied to N, $P_2O_5$ and $K_2O$ in golf course, N and $K_2O$ significantly affected water qualities of pond(P<0.01). These results suggested that a pond water in golf course was affected by the fertilizer application, rainfall and irrigation and EC was adequate monitoring indicator for the evaluating water quality of pond by chemicals influent.

• Journal of agricultural medicine and community health
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• v.25 no.2
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• pp.265-273
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• 2000

Benign prostatic hyperplasia (BPH) is a highly prevalent, age-related disorder in men which place a considerable burden on health care resources worldwide. While BPH and hypertension are apparently diverse disease processes, they have some features in common(e.g. underlying etiology of the sympathetic nervous system). The purpose of this study is to estimate the prevalence of lower urinary tract symptoms using I-PSS(international prostate symptom score) and to investigate the association of hypertension with I-PSS. This study was carried out to 390 men(40~86 year old) at 3 Myun in Chungju City from July to August, 1997. Subjects answered on questionnaire for I-PSS and were checked age, education, marital status, annual income, blood pressure. The mean age of subjects was 59.8 year old. The mean of I-PSS were increased by age decades(40~49, 50~59, 60~69, 70~86) and it's scores were 4.4, 7.0, 8.2, 12.3 respectively. Strong correlations were observed between the I-PSS and the QUL(quality of life)(P=0.0001). I-PSS(mean-value) were 7.85 in hypertensive group and 8.39 in normotensive group but there was no statistically significance between the two groups(P>0.05). The proportion of mean scoring greater than 8 was 42.5%. There have been reports of association between lower urinary tract symptoms and hypertension, but there was no consistent suggestion that such an association could be casual. The need for high-quality epidemiological information and consequent increased prospects for prevention is obvious.

• Korean Journal of Agricultural Science
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• v.9 no.2
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• pp.562-568
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• 1982

These studies were carried out to investigate of detection of Fasciola egg from positive cattle faces in the intradermal reaction method by laborsaving composition of sieves and Kim's sedimental tube method. The results obtained are summerized as follows: 1. Detection method of Fasciola eggs was improved by 3 meshes(100mesh, 150mesh, 250mesh) for filtration of fecal fluid contained Fasciola eggs. 2. III type of tube in the 3 kinds of sedimental tube had the highest recovery rate of Fasciola egg. Thus, this type of tube was selected and called as Kim's sedimental tube. 3. A-II, A-III, B-III and C-III type by Kim's sedimental tube method had the recovery rates of eggs in the 1st dropping as ranged 99 to 100% and A-I, B-II, C-I and C-II as 71 to 91%. 4. All types as themselves contained the special aspects. In the these type was useful to determine the results in the urgent cases add to shorten the time of the diagnostic procedures. 5. Kim's sedimental tube method high recovery rate and convenient procedures as compared with other detection methods reported. In addition, through this method can also obatained E. P. C. value. Therefore, it is desirable that Kim's sedimental tube method be recommended by clinicians.

• Korean Journal of Plant Resources
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• v.9 no.3
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• pp.285-290
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• 1996

This experiment was conducted to investigate the variation of adhering primary and secondary rachis branches of panicle in three ecotypes at National Honam Agricultural Experiment Station in 1993. Three ecotypes. Odaebyeo and Sinunbongbyeo as early-maturing type, Cheongmyeongbyeo and Changanbyeo as medium, and Dongjinbyeo and Mangeumbyeo as late-maturing type were used. The treatment were 5 planting times from May 5 to 5 July by 15 day intervals. The number of primary rachis branch in early maturing type recorded high in between May 5 and 20 May as early transplanting. Medium and late-maturing type, however, was found to be have more primary rachis branches at the late time of June 20 transplanting than at the optimum transplanting of Honam area. The number of secondary rachis branch was high between June 5 and 20 June regardless ecotypes. The rate of secondary rachis branch per primary rachis branch was increased with later transplanting time up to June 5, but showed no differences onwards. The number of grain in primary and secondary rachis branches were low in transplanting time of May 5 regardless ecotype but high in late transplanting time by July 5. Densinty of seed sets was found to be higher in late transplanting than in early transplating;early-maturing type showed high in July 5 and 20 June in medium-late maturing type respectively.

• Journal of Plant Biology
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• v.38 no.3
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• pp.305-317
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• 1995

The bryophyte communities on the forest floor of the mixed coniferous, deciduous broad-leaf forest and the evergreen coniferous forest of cool temperate and subalpine zones in Chiri Mountains with the highest peak (Cheonwangbong 1915 m) in south-central part of the Korean Peninsula were investigated from the phytosociology viewpoint. As a result, five bryophyte communities were recognized, considering the species composition of vascular vegetation; A. Sasamorpha borealis-Abies koreana/Dicranum scoparium-Plagiothecium silvaticum community, B. Bosta minor-Abies koreana/Anomodon rugelii-Plagiomnium cuspidatum community, C. Abies koreana/Hylocomiopsis ovicarpa-Entodon rubicundus community, D. Abies koreana-Picea jezoensis/Hylocomiopsis ovicarpa-Thuidium kanedae community, E. Abies koreana-Pieea jezoensis/Hylocomium splendens-Pleurozium sehreberi community. Among these, the communities A, B, C and D were distributed in the mixed coniferous and deciduous broad-leaf forest of lower altitudes (1200 to 1700 m) and characterized by the elements of the cool temperate zone. They were confimled as synusies belonging to the Fraxino-Abietetum koreanae Song 1988. The community E was distributed in higher altitudes (1700 m to near the summit) and characterized by the species group of the holarctic elements, e.g. Hyloeomium splendens, Pleurozium sehreberi, Dieranum majus, etc. limited in subarctic; subalpine and arctic; alpine zones. The community E was confirmed as a synusie of the Abieti koreanae-Piceetum jezoensis Song 1991 belonging the Vaecinio-Pieeetea Br.-BI. 1939. Up to date many investigator have described the coniferous forest distibuted above 1200 m in Chiri mountauns as the forest vegetation of subalpine zone. However, from the results of the present study, the boundary of the mixed forest and the true subalpine coniferous forest was elucidated to be ca. 1700 m phytosociologically. Species richness was highest in the community E, and the other four communities showed a similar species richness. Among the species diversity indices, H' and D were high in the communities Band E, low in the communities A and C, and medium in the community D. The values of J' decreased with the increase in altitudes and the community E showed the lowest value. The result of Bray-Curtis ordination revealed that the diversity analysis and the division of the above two forest zones are justifiable. The taxa of the bryophyte communities were composed of 25 families, 46 genera, 73 species, 1 subspecies and 2 varieties.

• Childhood Kidney Diseases
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• v.2 no.2
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• pp.104-109
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• 1998

Purpose : The proliferative nuclear antigen Ki-67, present in all cell cycle phases except G0, is a useful marker for the detection of proliferative cells in vivo. MIB I has been found to recognize an antigen in formalin-fixed and wax-embedded material. The aim of this study was to assess the efficacy of MIB-1 expression as a marker of representing the status of mesangial cell proliferation in renal tissues. Methods : Immunohistochemical staining for Ki-67 Ag using monoclonal antibody MIB-1 (Immunotech,505) were performed in 41 renal tissuses which were obtained by percutaneous renal biopsy done between January 1994 and December 1996. Results : In both glomeruli and renal tubules, MIB-1 expression was observed only in 2 of 18 ($11.1\%$) cases of IgA nephropathy, in 2 of the 4 ($50\%$) cases of mebranoproliferative glomerulonephritis, in 4 of the 5 ($80\%$) cases of poststreptococcal glomerulonephritis. But MIB-1 expression was not detected in all cases of minimal lesion and membranous nephropathy. Renal tubules In another 7 cases of IgA nephropathy were MIB-1 positive. Conclusion : MIB-1 expression in renal tissues may relate to the cell proliferation in glomeruli and renal tubules. But the efficacy of MIB-1 expression as a marker of mesangial cell proliferation may reveal a limited value because of it's lower positive rate in IgA nephropathy.