• Korean Journal of Soil Science and Fertilizer
• /
• v.29 no.4
• /
• pp.411-418
• /
• 1996

This experiment was conducted to investigate the effect of electric conductivity (EC) and potassium (K) concentration level in the culture solution on the growth and the air pollution monitoring capability of Perilla frutescens Suwon 16 which was recognized as a possible bioindicator for air pollution. The plants were exposed to $NO_2$, $O_3$, $SO_2$ and $NO_2+SO_2$ within a phytotron at $25^{\circ}C$ (day) or at $20^{\circ}C$(night) with 70% of relative humidity and evaluated the effect of EC and K level on the injury of the plants, The highest dry weights were gained as 16.3 g/plant at 0.5 dS/m of EC and as 32.3 g/plant at 100 and 200mg/l of K concentration, respectively. The diffusive resistances appeared as low values at 0.5 dS/m of EC and at 50, 100mg/l of K concentration. The increasing of the visible injury with increasing the dose of air pollutants could be taken at 0.5. 1.0 ds/m of EC and at 50, 100mg/l of K. The recommendable level of EC and K of the culture solution were 0.5-1.0 dS/m of electric conductivity. 50-100mg/l of K for the plant as an air pollutant biomonitor, when the 7th or 8th leaf was developed.

• Asian Pacific Journal of Cancer Prevention
• /
• v.13 no.8
• /
• pp.3781-3789
• /
• 2012

Background: Pancreatic cancer is one of the most aggressive tumors with a dismal prognosis. The membrane cytoskeletal crosslinker Ezrin participates in several functions including cell proliferation, adhesion, motility and survival. There is increasing evidence that Ezrin is overexpressed in vast majority of malignant tumors and regulates tumor progression. However, its roles in pancreatic cancer remain elusive. Methods: Three pairs of specific Ezrin siRNAs were designed and synthetized and screened to determine the most efficient one for construction of a hairpin RNA plasmid targeting Ezrin. After transfection into the Panc-1 pancreatic cancer cell line, real-time quantitative PCR and Western blotting were performed to examine the expression of mRNA and protein. The MTT method was applied to examine the proliferation and the drug sensibility to Gemcitabine. Flow cytometry was used to assess the cycle and apoptosis, while capacity for invasion was determined with transwell chambers. Furthermore, we detected phosphorylated-Erk1/2 protein and phosphorylated-Akt protein by Western blotting. Results: Real-time quantitative PCR and Western blotting revealed that Ezrin expression was notably down-regulated at both mRNA and protein levels by RNA interference (P< 0.01). Proliferation was inhibited and drug resistance to gemcitabine was improved (P< 0.05). Flow cytometry showed that the proportion of cells in the G1/G0 phase increased (P< 0.01), and in G2/M and S phases decreased (P< 0.05), with no apparent differences in apoptosis (P> 0.05). The capacity for invasion was markedly reduced (P< 0.01). In addition, down-regulating Ezrin expression had no effect on phosphorylated-Akt protein (P>0.05), but could decrease the level of phosphorylated-Erk1/2 protein (P< 0.05). Conclusions: RNA interference of Ezrin could inhibit its expression in the pancreatic cancer cells line Panc-1, leading to a potent suppression of malignant behavior in vitro. Assessment of potential as a target for pancreatic cancer treatment is clearly warranted.

• Journal of Life Science
• /
• v.19 no.6
• /
• pp.825-831
• /
• 2009

The purpose of this study was to investigate the influences of immune function and respiratory system on aerobic exercise of 75 %HRmax intensity in an exposed acute hypobaric hypoxic environment. The subjects were 20 male college students, 10 of which were soccer players (19.6${\pm}$0.8 yr, BMI 22.3${\pm}$1.0, V02max 56.5${\pm}$4.8 mljkgjmin) and 10 general students (22.2${\pm}$2.3 yr, BMI 22.7${\pm}$2.1, $VO_{2max}$ 50.5${\pm}$6.6 ml/kg/min). Items of measurement after exercise on level and high altitude were $O_{2}$, $CO_{2}$, ventilation (VE), respiratory frequency, respiratory quotient, RBC, MCV, MCH, MCHC, Hb, Hct, reticulocyte, WBC, neutrophil, lymphocyte, monocyte, basophil, and immunoglobulin (IgA, IgD, IgG, IgM). As the result of the verifying hypothesis, these results may suggest three new findings: first, to produce hypoxic ventilatory depression not only at level land but also at 3,000 m high altitude during 30 minute aerobics exercise of 75 %HRmax intensity, second to be more excellent for soccer players in adaptation and sensibility on response of respiratory system at high altitude, and third, to change each other by regular exercise habits or altitude in the monocyte.