• Title/Summary/Keyword: Fusion zone

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Ultrastructural Studies for Protoplasts and Protoplast Fusion in Streptomyces lavendulae (Streptomyces levendulae의 원형질체와 원형질체 융합에 대한 미세구조)

  • 하영칠;홍순우;유진철;임헌만
    • Korean Journal of Microbiology
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    • v.24 no.3
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    • pp.197-203
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    • 1986
  • Morphology and ultrastructure of protoplast fusion mode in Streptomyces lavendulae were studied by scanning and transmission electron microscopy. The isolated protoplasts were stable in some degree in hypertonic solution except that several protoplasts showed irregular morphology. Fusion events were occurred as follows; contact zone, fusion zone and separation zone were appeared sequentially. After formation of the separation zone, cytoplasm and DNA from both parents were mixed eventually. In the contact zone, two menbranes were still separated by electron transparent space. The contact zone changed to fusion zone by formation of fusion membrane that phospholipid molecules of two membranes were rearranged. Thereafter, nonmembraneous separation zone was formed by disappearance of fusion membrane. These changes were characterized by successive changes in typical membrane structure in fusion areas and by a progressive loss of bispherical shape.

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The effect of welding parameters on the formation of discontinuities in the laser fusion zone between Fe-Co-W sintered segment and mild steel (Fe-Co-W 소결체와 탄소강의 레이저 용융부 결함형성에 미치는 공정변수의 영향)

  • Kim S. W.;Yoon B. H.;Jung W. G.;Lee C. H.
    • Laser Solutions
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    • v.7 no.3
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    • pp.25-36
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    • 2004
  • This study was performed to clarification of the formation of weld discontinuities in the dissimilar laser fusion zone. Welding parameters were beam power of 1300, 1430, 1560, and 1700 W and travel speed of 1, 1.3, and 1.7 m/min. Most cavities in the fusion zone were observed near the tip. Cavities in the fusion zone observed to be formed and grown from pores in the tip. More cavities were formed as the beam position moves to the tip side. Small cavities were decreased but large cavities were increased when the energy input increased. W content in the fusion zone was increased with heat input and as the beam position close to the tip. In the fusion zone, W content in the dendrite boundary was increased with heat input. Considering the propagation path and fracture morphology, cracks were solidification cracking, and were initiated and propagated along the dendrite boundaries. The formation of cracks might be related with the W rich ${\mu}$ phase which was formed in the grain boundaries and dendrite boundaries.

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Microstructure and Tensile Strength Property of Arc Brazed DP steel using Cu-Sn Insert Metal (Cu-Sn 삽입금속을 이용한 DP강의 아크 브레이징 접합부의 미세조직과 인장특성)

  • Cho, Wook-Je;Cho, Young-Ho;Yun, Jung-Gil;Kang, Chung-Yun
    • Journal of Welding and Joining
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    • v.31 no.1
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    • pp.58-64
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    • 2013
  • The following results were obtained, microstructures and tensile properties in arc brazed joints of DP(dual phase) steel using Cu-5.3wt%Sn insert metal was investigated as function of brazing current. 1) The Fusion Zone was composed of ${\alpha}Fe+{\gamma}Cu$ and Cu23Sn2. The reason for the formation of these solid solutions. Despite, Fe & Cu were impossible to solid solution at room temperature. It's melting & reaction to something of insert metal & Base Metal (DP Steel) by Arc. Brazing Process has faster cooling rate then Cast Process, Supersaturated solid solution at room temperature. 2) The increase Hardness of Fusion Zone was directly proportional to the rise of welding current. Because, ${\alpha}Fe+{\gamma}Cu$ phase (higher hardness than the Cu23Sn2.(104.1Hv < 271.9Hv)) Volume fraction was Growth, due to increasing the amount of base metal melting by High current. 3) The results of tensile shear test by Brazing, All specimens happen to fracture in Fusion Zone. On the other hand, when Brazing Current increasing tend to rise tensile load. but it was very small, about 26-30% of the base metal. 4) The result of fracture analysis, The crack initiate at Triple Point for meet to Upper B.M/Under B.M/Fusion Zone. This Crack propagated to Fusion zone. So ruptured by tensile strength. The Reason to in the fusion zone fracture, Fusion zone by Brazing of hardness (strength) was very lower then the base metal (DP steel). In addition the Fusion Zone's thickness in triple point was thin than the base metal's thickness in triple point.

Microstructure and Mechanical Properties of Gas Metal Arc Brazed Joint of DP Steel with Cu-Si Filler Metal (Cu-Si 삽입금속을 이용한 DP강의 MIG 아크 브레이징 접합부의 미세조직과 기계적 성질)

  • Cho, Wook-Je;Yoon, Tae-Jin;Kwak, Sung-Yun;Lee, Jae-Hyeong;Kang, Chung-Yun
    • Journal of Welding and Joining
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    • v.34 no.5
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    • pp.70-76
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    • 2016
  • In this study, Microstructure and tensile properties in arc brazed joints of 1000MPa grade DP steel using Cu-Si insert metal were investigated. The fusion zone was composed of Cu phase which solidified a little Fe and Si. The former phase formed due to dilute the edge of base material by arc, although Fe was not solid solution in Cu at the room temperature. Cu3Si particles formed by crystallization at $1100^{\circ}C$ during faster cooling. After the tensile shear test, there are no differences between the brazed joint efficiencies. The maximum joint efficient was about 37% compared to strength of base metal. It is better than that of arc brazed joint of DP steel using Cu-Sn filler metal. Fracture position of all brazing conditions was in the fusion zone. Crack initiation occurred at three junction point which was a stress singularity point of upper sheet, lower sheet and the fusion zone. And then crack propagated across the fusion zone. The reason why the fracture occurred at fusion zone was that the hardness of fusion zone was lower than that of base material and heat affected zone. The correlation among maximum load and hardness of fusion zone and EST at fractured position was $R^2=0.9338$. Therefore, this means that hardness and EST can have great impact on maximum load.

Mechanism of Hot Cracking in High Strength Al Welds (고강도 알루미늄합금 용접부의 고온균열 Mechanism)

  • 이창희;조성석
    • Journal of Welding and Joining
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    • v.14 no.3
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    • pp.93-104
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    • 1996
  • This study evaluated relative hot cracking susceptibility of commercial aluminum alloy welds, and then suggested possible mechanisms operated in the weld fusion zone and in the heat affected zone based on the observed cracking morphologies, fractography and microstructural features. The fusion zone solidification cracking was found to be mainly due to a microsegregation of Cu, Si, and Mg in grain boundaries, while liquation cracking in the HAZ was by the incipient melting of the segregated grain boundaries and the consitutional liquation of large aging precipitates and intermetallic compounds in the partially melted zone adjacent to the fusion line which experienced a rapid thermal excursion during welding.

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Effect of Hot-stamping on Microstructures and Tensile Properties of Al-Si Coated Boron Steel Welds with Laser Source (Al-Si 도금된 보론강 레이저 소스에 따른 레이저 용접부의 미세조직과 기계적 성질에 미치는 핫스탬핑 처리의 영향)

  • Oh, Myeong-Hwan;Kong, Jong-Pan;Kwon, Min-Suck;Kang, Chung-Yun
    • Journal of Welding and Joining
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    • v.31 no.6
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    • pp.96-106
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    • 2013
  • In this study, the effect of laser source($CO_2$ and Nd:YAG) on the microstructure and tensile properties of laser welded Al-Si coated boron steel(1.2mmt) was investigated with before and after hot-stamping. In case of as welds condition, fracture occurred in base metal unrelated to the laser source. It could be explained that tensile strength of fusion zone composed of martensite and bainite is higher than that of base metal that contains a lot of ferrite despite dilution of Al and Si from coating layer to fusion zone. In case of hot-stamping condition, the fracture occurred in fusion zone irrelevant to laser source and the tensile strength was lower than hot stamped base metal. In the $CO_2$ laser welds, $Fe_3$(Al,Si) formed near the bond line was transformed into ferrite during hot-stamping. Therefore tensile strength of bond line is lower than that of base metal and center of fusion zone and the fracture occurred in the bond line. On the other hand, in the Nd:YAG laser welds, the higher concentration of Al formed the ferrite in the fusion zone during hot-stamping treatment. Also, the thickness of centerline was thinner than that of base metal. Therefore, it is considered that fracture occurred in centerline of fusion zone due to effect of concentration stress, and it leaded to a lower tensile strength and elongation.

Effect of Coating Thickness on Microstructures and Tensile Properties in Yb:YAG Disk Laser Welds of Al-Si Coated Boron Steel (Al-Si 용융 도금된 보론강의 Yb:YAG 디스크 레이저 용접부의 미세조직과 인장성질에 미치는 도금두께의 영향)

  • Cao, Wei-Ye;Kong, Jong-Pan;Ahn, Yong-Nam;Kim, Cheol-Hee;Kang, Chung-Yun
    • Journal of Welding and Joining
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    • v.31 no.3
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    • pp.66-75
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    • 2013
  • In this study, the effect of coating thickness($20{\mu}m$ and $30{\mu}m$) on microstructure and tensile properties in Yb:YAG disk laser welds of Al-Si-coated boron steel (1.2mmt) was investigated. In the case of as welds, the quantity of ferrite was found to be higher in base metal than that in HAZ (Heat Affected Zone) and fusion zone, indicating, fracture occurrs in base metal, and the fracture position is unrelated to the coating thickness. Furthermore, yield strength, tensile strength of base metal and welded specimens showed similar behavior whereas elongation was decreased. On the other hand, base metal and HAZ showed existence of martensite after heat treatment, the fusion zone indicated the presence of full ferrite or austenite and ferrite during heat treatment ($900^{\circ}C$, 5min), After water cooling, austenite was transformed to martensite, and the quantity of ferrite in fusion zone was higher as compared with in base metal, resulting in sharply decrease of yield strength, tensile strength and elongation, which leads to fracture occured at fusion zone. In particular, results showed that because the concentration of Al was higher in 30um coating layer specimen than that of 20um coating specimen, after heat treatment, producing a higher quantity of ferrite was higher after heat treatment in the fusion zone; howevers, it leads to a lower tensile property.

Simulation of an X-ray Fresnel Zone Plate with Nonideal Factors

  • Chen, Jie;Fan, Quanping;Wang, Junhua;Yuan, Dengpeng;Wei, Lai;Zhang, Qiangqiang;Liao, Junsheng;Xu, Min
    • Current Optics and Photonics
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    • v.4 no.1
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    • pp.9-15
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    • 2020
  • Fresnel zone plates have been widely used in many applications, such as x-ray telescopes, microfluorescence, and microimaging. To obtain an x-ray Fresnel zone plate, many fabrication methods, such as electron-beam etching, ion-beam etching and chemical etching, have been developed. Fresnel zone plates fabricated by these methods will inevitably lead to some nonideal factors, which have an impact on the focusing characteristics of the zone plate. In this paper, the influences of these nonideal factors on the focusing characteristics of the zone plate are studied systematically, by numerical simulations based on scalar diffraction theory. The influence of the thickness of a Fresnel zone plate on the absolute focusing efficiency is calculated for a given incident x-ray's wavelength. The diffraction efficiency and size of the focal spot are calculated for different incline angles of the groove. The simulations of zone plates without struts, with regular struts, and with random struts are carried out, to study the effects of struts on the focusing characteristics of a zone plate. When a Fresnel zone plate is used to focus an ultrashort x-ray pulse, the effect of zone-plate structure on the final pulse duration is also discussed.

Anastomosis Types and Hyphal Interactions among Different Location and Field Isolates of Rhizoctonia solani AG-1(IA), R. oryzae and R. oryzae-sativae (Rhizoctonia solani AG-1(IA), R. oryzae, R. oryzae-sativae의 다른 지역(地域) 및 포장분리(圃場分離) 균주(菌株)들간의 균사융합형(菌絲融合型)과 균사상호작용(菌絲相互作用))

  • Kim, Wan-Gyu
    • The Korean Journal of Mycology
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    • v.21 no.3
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    • pp.188-194
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    • 1993
  • Anastomosis types and hyphal interactions in culture among different location and field isolates of Rhizoctonia solani AG-1(IA), R. oryzae and R. oryzae-sativae were examined. In the pairings of R. solani AG-1(IA) isolates, cytoplasmic fusion only occurred in the self-anastomoses, and non-cytoplasmic fusion occurred in the other combinations. In the pairings of R. oryzae isolates, cytoplasmic fusion occurred in six combinations between different location isolates and in two combinations between different field isolates from the same locations as well as in the self-anastomoses. In that case, four isolates of the fungus reciprocally made the cytoplasmic fusion. In the pairings of R. oryzae-sativae isolates, only non-cytoplasmic fusion occurred among the different location and field isolates, in which cytoplasmic fusion also occurred in the self-anastomoses. When non-cytoplasmic fusion isolates(NCFIs) of R. solani AG-1(IA) were opposed on PDA, a killing zone developed between the NCFls paired after incubation. The killing zone also developed between the NCFls of R. oryzae paired. No killing zone developed between the cytoplasmic fusion isolates(CFIs) of R. oryzae, in which mycelia of the CFIs intermingled with each other without formation of any demarcation line. An entangled zone instead of the killing zone developed between the NCFIs of R. oryzae-sativae.

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Hardness Distribution and Microstructures of Electric Resistance Spot Welded 1GPa Grade Dual Phase Steel (1GPa급 DP강 전기저항점용접부의 경도분포와 미세조직의 상관관계)

  • Na, Hye-Sung;Kong, Jong-Pan;Han, Tae-Kyo;Chin, Kwang-Geun;Kang, Chung-Yun
    • Journal of Welding and Joining
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    • v.30 no.2
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    • pp.76-80
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    • 2012
  • In this study, the effect of the welding current on the hardness characteristics and microstructure in the resistance spot welding of 1GPa grade cold-rolled DP steel was investigated, Also, correlation between the hardness and microstructure was discussed. In spite of the change in the welding current, the hardness distributions near weld was similar. the hardness in the HAZ and the fusion zone was higher than that of the base metal and the hardness in the fusion zone was variated with the location. Especially, the hardness of HAZ adjacent to the base metal showed maximum value, and softening zone in the base metal adjacent to HAZ was found. With the increasing of welding current, there were no difference in maximum hardness and average hardness in the fusion zone were, but the hardness of the softening zone reduced. The difference in the hardness in each location of weld due to grain size of prior austenite. The softening of the base metal occurred by tempering of the martensite.