• IMMUNE NETWORK
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• v.2 no.4
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• pp.233-241
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• 2002

Background: Monoclonal antibodies (mAb) of rodent origin are produced with ease by hybridoma fusion technique, and have been successfully used as therapeutic reagents for humans after humanization by genetic engineering. However, utilization of these antibodies for therapeutic purpose has been limited by the fact that they act as immunogens in human body causing undesired side effects. So far, there have been several attempts to produce human mAbs for effective in vivo diagnostic or therapeutic reagents including the use of humanized xenomouse that is generated by mating knockout mice which lost Ig heavy and light chain genes by homologous recombination and transgenic mice having both human Ig heavy and light gene loci in their genome. Methods: Genomic DNA fragments of mouse Ig heavy and light chain were obtained from a mouse brain ${\lambda}$ genomic library by PCR screening and cloned into a targeting vector with ultimate goal of generating Ig knockout mouse. Results: Through PCR screening of the genomic library, three heavy chain and three light chain Ig gene fragments were identified, and restriction map of one of the heavy chain gene fragments was determined. Then heavy chain Ig gene fragments were subcloned into a targeting vector. The resulting construct was introduced into embryonic stem cells. Antibiotic selection of transfected cells is under the progress. Conclusion: Generation of xenomouse is particularly important in medical biotechnology. However, this goal is not easily achieved due to the technical difficulties as well as huge financial expenses. Although we are in the early stage of a long-term project, our results, at least, partially contribute the successful generation of humanized xenomouse in Korea.

• Journal of Digital Convergence
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• v.16 no.9
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• pp.413-419
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• 2018

Nowadays, smart phone became an important part of communication media and integrated into people's life. If callers rely on helmet-mounted display(HMD) augmented reality technique to add two-way user's facial expression, appearance, actions during the calling process, it will let callers have a visualized fantastic sensual experience. And through that method can break the limitations of vision, so research that technical problem can promote the development of visual arts, that is meaningful. This paper will choose and composite several existed technologies to set up two hypothesis, try to realize AR video calling. Through comparison and analysis to find those two hypothesis' problem, and create design solutions to solve problems. And use case study method to present two cases for prove my paper's result that is those two hypothesis can be realize in future. Use those technologies can bring more convenience and enjoyment to people's life. It can be predicted that AR video calling process can be successfully realized and will have unlimited development in future.