• BMB Reports
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• v.35 no.3
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• pp.320-329
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• 2002

A gene, coined tay, for a thermostable DNA polymerase from the novel, extremely thermophilic bacterium Thermoanaerobacter yonseiensis was cloned and expressed in E. coli. Using a DNA polymerase homologous PCR product as a hybridization probe, tay was isolated and sequenced to consist of 2621 nucleotides that encode 872 amino acids. A database analysis showed that DNA polymerase, coined Tay, from T. yonseiensis shared a 39% to 47% identity in the amino acid sequence with those from other DNA polymerases. Tay was overexpressed in E. coli as a fusion protein with a poly-histidine tag at the C-terminus. It was purified by heat treatment, followed by a $Ni^{2+}$-chelate column. The molecular weight of purified Tay was approximately 97 kDa, as shown by SDS PAGE, and it showed high DNA polymerase activity and thermostability. However, it had no 3'$\rightarrow$5' exonuclease activity.

• Journal of Information Technology Services
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• v.15 no.1
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• pp.167-178
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• 2016

Spectrum sensing in cognitive radio (CR) has a great role in order to utilize idle spectrum opportunistically, since it is responsible for making available dynamic spectrum access efficiently. In this research area, collaboration among multiple cognitive radio users has been proposed for the betterment of detection reliability. Even though cooperation among them improves the spectrum sensing performance, some falsely reporting malicious users may degrade the performance rigorously. In this article, we have studied the detection and nullifying the harmful effects of such malicious users by applying some well known outlier detection methods based on Grubb's test, Boxplot method and Dixon's test in cooperative spectrum sensing. Initially, the performance of each technique is compared and found that Boxplot method outperforms both Grubb's and Dixon's test for the case where multiple malicious users are present. Secondly, a new algorithm based on reputation and weight is developed to identify malicious users and cancel out their negative impact in final decision making. Simulation results demonstrate that the proposed scheme effectively identifies the malicious users and suppress their harmful effects at the fusion center to decide whether the spectrum is idle.

• Journal of Pharmaceutical Investigation
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• v.17 no.4
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• pp.175-181
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• 1987

To increase the dissolution rate of furosemide, chitin and chitosan which are widely occurring biodegradable natural materials were used as drug carriers. The ground mixtures of furosemide with chitin or chitosan were prepared by grinding in a ball mill. The ground mixture showed a faster and more enhanced dissolution rate than the physical mixture or intact furosemide. The crystalline peaks of furosemide disappeared in the ground mixtures indicating the production of amorphous form. The comparison of infrared spectra of the physical mixture and the ground mixture showed an interaction such as association between the functional groups of furosemide and chitin or chitosan in the molecular level. The weight losses in TGA curves showed all the same patterns. However, the endothermic peak due to the fusion of furosemide in DTA curve disappeared in the ground mixture indicating the different thermal property. The dissolution of furosemide from ground mixtures was fast in the order of chitosan and then chitin. The co-grinding technique with chitin or chitosan provided a promising way enhancing the dissolution rate of practically insoluble drug.

• Korean Journal of Pharmacognosy
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• v.27 no.3
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• pp.224-230
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• 1996

To find pharmacologically active hybrids among the inter-order protoplast fusants of Lentinula edodes and Ginoderma lucidam the antitumor test was performed and the fusant P22 was selected among them. The hot water and alkaline extracts from the cultured mycelia of P22 were purified and separated into four fractions by DEAE-cellulose anion exchange chromatography. When a dose of 20 mg/kg/day of each fractions was injected into ICR mice by i.p., the tumor inhibition ratio of Fr. IV against solid sarcoma 180 was the higher than any other fraction. Fr. IV was a protein-bound polysaccharide which was composed of 69. 12% polysacchafide and 9.76% protein and the molecular weight of Fr. IV was $6.7{\times}10^4$ dalton.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2006.05a
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• pp.367-368
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• 2006

Today, the Artificial Intervertebral Disc (AID) is being developed by increasing the oblique of the endplate gradually. In other words, Ultra-high Molecular Weight Polyethylene (UHMWPE) which is apply to the sliding core of the AID, does not change the shape but alters the oblique of endplate. However, the unreasonable increase of degree of freedom (DOF) can result in the aggravation of the bone fusion and the initial stability and it can also lead to the increase of the concentrated force in core. For these reasons, it is necessary to develop the advanced techniques, which choose the most adequate DOF. In this study, the new optimized modeling of the sliding core and the endplate, the fatigue characteristics, the crack propagation and the formation mechanism of wearing debris was studied and the minimizing technique will be derived from this research.

• Proceedings of the KWS Conference
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• 2005.06a
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• pp.370-376
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• 2005

Magnesium alloys are becoming important material for light weight car body, due to their low specific density but high specific strength. However they have a poor weldability, caused high oxidization tendency and low vapor temperature. In this study, the welding performance of magnesium alloys was investigated for automobile application. The materials were rolled magnesium alloy sheet contains Al and Zn such as AZ3l , AZ6l and AZ9l. Three types of welding process were studied, that were GTAW, Laser beam welding and FSW. To evaluate the weldability, we examined the appearance of welding bead. Also we checked bead shape and internal defects such as crack and porosity on cross section of welding bead. The mechanical property was measured for welded specimen by tensile test. For determination of the strength change by welding process, the hardness profile across the welding center was measured. For the results, the tensile properties of welded specimen were decreased obviously on all welding process. For the fusion welding process such as GTAW and laser beam welding, the surface of the welding bead was covered with oxidized magnesium dust but it was removed by simple cleaning work as wipe-out with tissue. Also under cut, that caused vaporization of base metal was occurred. for the friction stir welding, there was no oxidation, under-cut or internal defects. However it had poor weld performance, the reason was cleavage fracture occurred at plastic deformation zone. For welding of magnesium alloy, the laser beam welding process was recommended.

• Korean Journal of Agricultural Science
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• v.41 no.3
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• pp.237-243
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• 2014

Human Glucagon like peptide-1 (GLP-1) is an incretin hormone that promotes secretion of insulin. In order to eliminate the formation of the soluble aggregate, Ala19 in GLP-1 was substituted with Thr, resulting in a GLP-1 mutant GLP-1A19T. The gene synthesis of GLP-1A19T and the fusion of 6-lysine tagged ubiquitin gene were accomplished by using the overlap extension polymerase chain reaction. The ubiquitin fused GLP-1A19T (K6UbGLP-1A19T) is expressed as form of inclusion body with little formation of the soluble aggregation in recombinant E. coli. In order to produce K6UbGLP-1A19T in large amounts, fed-batch fermentation was carried out in a pH-stat feeding strategy. Maximum dry cell weight of 87.7 g/L and 20.4% of specific K6UbGLP-1A19T content were obtained. Solid-phase refolding using a cation exchanger was carried out to renature K6UbGLP-1A19T. The refolded K6UbGLP-1A19T aggregated little and was released GLP-1A19T by on-column cleavage with ubiquitin-specific protease-1. The molecular mass of GLP-1A19T showed an accurate agreement with its theoretical molecular mass.

• Journal of Electrical Engineering and Technology
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• v.3 no.2
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• pp.167-176
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• 2008

Vulnerability assessment of power systems is important so as to determine their ability to continue to provide service in case of any unforeseen catastrophic contingency such as power system component failures, communication system failures, human operator error, and natural calamity. An approach towards the development of on-line power system vulnerability assessment is by means of using an artificial neural network(ANN), which is being used successfully in many areas of power systems because of its ability to handle the fusion of multiple sources of data and information. An important consideration when applying ANN in power system vulnerability assessment is the proper selection and dimension reduction of training features. This paper aims to investigate the effect of using various feature extraction methods on the performance of ANN as well as to evaluate and compare the efficiency of the proposed feature extraction method named as neural network weight extraction. For assessing vulnerability of power systems, a vulnerability index based on power system loss is used and considered as the ANN output. To illustrate the effectiveness of ANN considering various feature extraction methods for vulnerability assessment on a large sized power system, it is verified on the IEEE 300-bus test system.

• Journal of Sensor Science and Technology
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• v.22 no.5
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• pp.364-368
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• 2013

Vibrational energy harvester based on piezoelectricity has been expected to be the dominant energy harvesting technology due to the advantages of high conversion efficiency, light weight and small size, night operation, etc. Its commercialization is just around the corner but the integration with power management electronics should be solved in advance. In this paper, therefore, fully-integrated design environment for piezoelectric energy harvesting systems is presented to assist co-design with the power management electronics. The proposed design environment is capable of analyzing the energy harvester including the package-induced damping effects and simulating the device and its power management electronics simultaneously. When the developed design environment was applied to the fabricated device, the simulated resonant frequency matched well with the experimental result with a difference of 2.97% only. Also, the complex transient response was completed in short simulation time of 3,001 seconds including the displacement distribution over the device geometry. Furthermore, a full-bridge power management circuit was modeled and simulated with the energy harvester simultaneously. Therefore the proposed, fully-integrated design environment is accurate and fast enough for the contribution on successful commercialization of piezoelectric energy harvester.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.33 no.3
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• pp.173-179
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• 2015

In this paper, object-based classification of urban areas based on a combination of information from lidar and aerial images is introduced. High resolution images are frequently used in automatic classification, making use of the spectral characteristics of the features under study. However, in urban areas, pixel-based classification can be difficult since building colors differ and the shadows of buildings can obscure building segmentation. Therefore, if the boundaries of buildings can be extracted from lidar, this information could improve the accuracy of urban area classifications. In the data processing stage, lidar data and the aerial image are co-registered into the same coordinate system, and a local maxima filter is used for the building segmentation of lidar data, which are then converted into an image containing only building information. Then, multiresolution segmentation is achieved using a scale parameter, and a color and shape factor; a compactness factor and a layer weight are implemented for the classification using a class hierarchy. Results indicate that lidar can provide useful additional data when combined with high resolution images in the object-oriented hierarchical classification of urban areas.